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Protein

Elongation factor 1-alpha

Gene

tuf

Organism
Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi13 – 208GTPUniRule annotation
Nucleotide bindingi90 – 945GTPUniRule annotation
Nucleotide bindingi152 – 1554GTPUniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Elongation factor

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciAPER272557:GJD6-1246-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor 1-alphaUniRule annotation
Short name:
EF-1-alphaUniRule annotation
Alternative name(s):
Elongation factor TuUniRule annotation
Short name:
EF-TuUniRule annotation
Gene namesi
Name:tufUniRule annotation
Ordered Locus Names:APE_1844
OrganismiAeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1)
Taxonomic identifieri272557 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiDesulfurococcalesDesulfurococcaceaeAeropyrum
Proteomesi
  • UP000002518 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 437437Elongation factor 1-alphaPRO_0000090974Add
BLAST

Proteomic databases

PRIDEiQ9YAV0.

Interactioni

Protein-protein interaction databases

DIPiDIP-59399N.
STRINGi272557.APE_1844.

Structurei

Secondary structure

1
437
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi6 – 127Combined sources
Helixi19 – 2911Combined sources
Helixi35 – 4713Combined sources
Helixi51 – 533Combined sources
Helixi56 – 594Combined sources
Helixi63 – 686Combined sources
Beta strandi75 – 806Combined sources
Beta strandi85 – 906Combined sources
Helixi95 – 973Combined sources
Helixi98 – 1069Combined sources
Beta strandi109 – 1168Combined sources
Helixi121 – 1255Combined sources
Helixi131 – 14111Combined sources
Beta strandi146 – 1527Combined sources
Turni157 – 1615Combined sources
Helixi163 – 17917Combined sources
Helixi184 – 1863Combined sources
Beta strandi189 – 1913Combined sources
Turni194 – 1963Combined sources
Turni198 – 2003Combined sources
Helixi215 – 2206Combined sources
Beta strandi234 – 2363Combined sources
Beta strandi238 – 2436Combined sources
Turni244 – 2463Combined sources
Beta strandi247 – 2537Combined sources
Beta strandi264 – 2685Combined sources
Turni269 – 2713Combined sources
Beta strandi272 – 28110Combined sources
Beta strandi284 – 2896Combined sources
Beta strandi294 – 3007Combined sources
Helixi304 – 3063Combined sources
Beta strandi312 – 3187Combined sources
Beta strandi322 – 33211Combined sources
Beta strandi346 – 3494Combined sources
Beta strandi352 – 36514Combined sources
Turni367 – 3693Combined sources
Beta strandi372 – 3765Combined sources
Beta strandi385 – 39410Combined sources
Turni401 – 4033Combined sources
Helixi405 – 4073Combined sources
Beta strandi408 – 4147Combined sources
Beta strandi417 – 42913Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3VMFX-ray2.30A1-437[»]
3WXMX-ray2.30A/C/E/G1-437[»]
4CXGelectron microscopy8.70A1-437[»]
4CXHelectron microscopy8.90A1-437[»]
ProteinModelPortaliQ9YAV0.
SMRiQ9YAV0. Positions 4-430.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 229226tr-type GAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni13 – 208G1By similarity
Regioni69 – 735G2By similarity
Regioni90 – 934G3By similarity
Regioni152 – 1554G4By similarity
Regioni193 – 1953G5By similarity

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01561. Archaea.
COG5256. LUCA.
HOGENOMiHOG000229291.
KOiK03231.
OMAiPASTIFH.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00118_A. EF_Tu_A.
InterProiIPR004161. EFTu-like_2.
IPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
IPR004539. Transl_elong_EF1A_euk/arc.
IPR004160. Transl_elong_EFTu/EF1A_C.
[Graphical view]
PfamiPF03144. GTP_EFTU_D2. 1 hit.
PF03143. GTP_EFTU_D3. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00483. EF-1_alpha. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9YAV0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEKPHMNLV VIGHVDHGKS TLVGHLLYRL GYIEEKKLKE LEEQAKSRGK
60 70 80 90 100
ESFKFAWILD KMKEERERGI TIDLTFMKFE TKKYVFTIID APGHRDFVKN
110 120 130 140 150
MITGASQADA AILVVSARKG EFEAGMSTEG QTREHLLLAR TMGIEQIIVA
160 170 180 190 200
VNKMDAPDVN YDQKRYEFVV SVLKKFMKGL GYQVDKIPFI PVSAWKGDNL
210 220 230 240 250
IERSPNMPWY NGPTLVEALD QLQPPAKPVD KPLRIPVQNV YSIPGAGTVP
260 270 280 290 300
VGRVETGVLR VGDKVVFMPP GVVGEVRSIE MHYQQLQQAE PGDNIGFAVR
310 320 330 340 350
GVSKSDIKRG DVAGHLDKPP TVAEEFEARI FVIWHPSAIT VGYTPVIHVH
360 370 380 390 400
TASVSSRIIE IKAKLDPKTG QVVEQNPQFL KAGDAAIVRF KPVKPLVVEK
410 420 430
FSEIPQLGRF AMRDMNRTVG IGIVTDVKPA KVDIKAK
Length:437
Mass (Da):48,663
Last modified:November 1, 1999 - v1
Checksum:iD6AE87FEB8AD003C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000002 Genomic DNA. Translation: BAA80848.1.
PIRiC72570.

Genome annotation databases

EnsemblBacteriaiBAA80848; BAA80848; APE_1844.
KEGGiape:APE_1844.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000002 Genomic DNA. Translation: BAA80848.1.
PIRiC72570.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3VMFX-ray2.30A1-437[»]
3WXMX-ray2.30A/C/E/G1-437[»]
4CXGelectron microscopy8.70A1-437[»]
4CXHelectron microscopy8.90A1-437[»]
ProteinModelPortaliQ9YAV0.
SMRiQ9YAV0. Positions 4-430.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-59399N.
STRINGi272557.APE_1844.

Proteomic databases

PRIDEiQ9YAV0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA80848; BAA80848; APE_1844.
KEGGiape:APE_1844.

Phylogenomic databases

eggNOGiarCOG01561. Archaea.
COG5256. LUCA.
HOGENOMiHOG000229291.
KOiK03231.
OMAiPASTIFH.

Enzyme and pathway databases

BioCyciAPER272557:GJD6-1246-MONOMER.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00118_A. EF_Tu_A.
InterProiIPR004161. EFTu-like_2.
IPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
IPR004539. Transl_elong_EF1A_euk/arc.
IPR004160. Transl_elong_EFTu/EF1A_C.
[Graphical view]
PfamiPF03144. GTP_EFTU_D2. 1 hit.
PF03143. GTP_EFTU_D3. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00483. EF-1_alpha. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1.

Entry informationi

Entry nameiEF1A_AERPE
AccessioniPrimary (citable) accession number: Q9YAV0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1999
Last modified: July 6, 2016
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.