Reviewed, UniProtKB/Swiss-Prot Q9YAS4 (HMDH_AERPE)

Names and origin

Protein names

Recommended name:
    3-hydroxy-3-methylglutaryl-coenzyme A reductase
      Short name=HMG-CoA reductase
    EC=1.1.1.34

Gene names

Name:	hmgA
Ordered Locus Names:	APE_1869

Organism

Aeropyrum pernix [Complete proteome] [HAMAP]

Taxonomic identifier

56636 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Desulfurococcales › Desulfurococcaceae › Aeropyrum

Protein attributes

Sequence length	421 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Converts HMG-CoA to mevalonate By similarity.
Catalytic activity	(R)-mevalonate + CoA + 2 NADP+ = (S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH.
Pathway	Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 3/3.
Sequence similarities	Belongs to the HMG-CoA reductase family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	coenzyme A metabolic process Inferred from electronic annotation. Source: InterPro isoprenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: InterPro
Molecular function	NADP or NADPH binding Inferred from electronic annotation. Source: InterPro hydroxymethylglutaryl-CoA reductase (NADPH) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 421	421	3-hydroxy-3-methylglutaryl-coenzyme A reductase		PRO_0000114458
Sites
	Active site	109	1	Charge relay system By similarity
	Active site	240	1	Charge relay system By similarity
	Active site	315	1	Charge relay system By similarity
	Active site	410	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9YAS4-1 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: 9F67AF57FA651822	FASTA	421	44,625
	10 20 30 40 50 60 MGSSSGQKPR RLEDLVDKLA SGSLSHSRLE KELGNANEAA LVRRLYLERL TGASLSSVAS 70 80 90 100 110 120 TILDFQELYG RNIENPIGAV QVPVGVAGPL RINGDYARGD FYIPLATTEG ALVASVNRGA 130 140 150 160 170 180 KAITLSGGAR AKVIKDGMTR APLLWTPSVY EAHRLAMWVE DRIEDLRSVV AGVTRHGRLQ 190 200 210 220 230 240 HIYPYIIGNL VWLRLSFSTG DAMGMNMVTI SSDRICRYIE ENYDGDAKCI ALSGNMCTDK 250 260 270 280 290 300 KPAAINKILG RGKYVVAEAV IKGEVVKNVL KTTPQNINLV NVTKNLLGSA AAGSHSFNAH 310 320 330 340 350 360 FANIIAAIFI ATGQDAAQVV ESSMGYTWTE VRGEDLYISV TLPSLEVGTV GGGTRLPTQR 370 380 390 400 410 420 ELLALLGVAG GGNPPGSNAL KLAEIIASAV LAGELNLLSA IAAGQLARAH ELLGRGGLKI S

References

[1]

"Complete genome sequence of an aerobic hyper-thermophilic crenarchaeon, Aeropyrum pernix K1." Kawarabayasi Y., Hino Y., Horikawa H., Yamazaki S., Haikawa Y., Jin-no K., Takahashi M., Sekine M., Baba S., Ankai A., Kosugi H., Hosoyama A., Fukui S., Nagai Y., Nishijima K., Nakazawa H., Takamiya M., Masuda S. , Funahashi T., Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Kubota K., Nakamura Y., Nomura N., Sako Y., Kikuchi H. DNA Res. 6:83-101(1999) [PubMed: 10382966] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000002 Genomic DNA. Translation: BAA80874.1.
PIR	E72573.
RefSeq	NP_148225.1.
3D structure databases
SMR	Q9YAS4. Positions 12-406.
ModBase	Search...
Genome annotation databases
GeneID	1446303.
GenomeReviews	Gene locus APE_1869 in contig BA000002_GR.
KEGG	ape:APE_1869.
NMPDR	fig|272557.1.peg.1333.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG604528.
OMA	AHFANII.
Enzyme and pathway databases
BioCyc	APER272557:APE1869-MONOMER.
BRENDA	1.1.1.34. 256344.
Family and domain databases
InterPro	IPR002202. HMG_CoA_Rdtase_cat. IPR004554. HMG_CoA_Rdtase_I_cat. IPR009023. HMG_CoA_Rdtase_NAD(P)-bd. IPR009029. HMG_CoA_Rdtase_sub-bd. [Graphical view]
Gene3D	G3DSA:3.90.770.10. HMG-CoA_red. 1 hit.
PANTHER	PTHR10572. HMG-CoA_red. 1 hit.
Pfam	PF00368. HMG-CoA_red. 1 hit. [Graphical view]
PRINTS	PR00071. HMGCOARDTASE.
TIGRFAMs	TIGR00533. HMG_CoA_R_NADP. 1 hit.
PROSITE	PS00066. HMG_COA_REDUCTASE_1. 1 hit. PS00318. HMG_COA_REDUCTASE_2. 1 hit. PS01192. HMG_COA_REDUCTASE_3. False negative. PS50065. HMG_COA_REDUCTASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HMDH_AERPE

Accession

Primary (citable) accession number: Q9YAS4

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	November 1, 1999
Last modified:	February 9, 2010
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)