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Q9YAQ8

- MTAP_AERPE

UniProt

Q9YAQ8 - MTAP_AERPE

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Protein

S-methyl-5'-thioadenosine phosphorylase

Gene

mtnP

Organism
Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the reversible phosphorylation of S-methyl-5'-thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S-adenosylmethionine. Has broad substrate specificity with 6-aminopurine nucleosides as preferred substrates.UniRule annotation

Catalytic activityi

S-methyl-5'-thioadenosine + phosphate = adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei20 – 201PhosphateUniRule annotation
Sitei176 – 1761Important for substrate specificityUniRule annotation
Binding sitei195 – 1951Substrate; via amide nitrogenUniRule annotation
Binding sitei196 – 1961PhosphateUniRule annotation
Sitei230 – 2301Important for substrate specificityUniRule annotation

GO - Molecular functioni

  1. phosphorylase activity Source: InterPro
  2. S-methyl-5-thioadenosine phosphorylase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. L-methionine biosynthetic process from methylthioadenosine Source: UniProtKB-HAMAP
  2. purine ribonucleoside salvage Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Purine salvage

Enzyme and pathway databases

BioCyciAPER272557:GJD6-1277-MONOMER.
UniPathwayiUPA00904; UER00873.

Names & Taxonomyi

Protein namesi
Recommended name:
S-methyl-5'-thioadenosine phosphorylaseUniRule annotation (EC:2.4.2.28UniRule annotation)
Alternative name(s):
5'-methylthioadenosine phosphorylaseUniRule annotation
Short name:
MTA phosphorylaseUniRule annotation
Short name:
MTAPUniRule annotation
Gene namesi
Name:mtnPUniRule annotation
Ordered Locus Names:APE_1885
OrganismiAeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1)
Taxonomic identifieri272557 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiDesulfurococcalesDesulfurococcaceaeAeropyrum
ProteomesiUP000002518: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 275275S-methyl-5'-thioadenosine phosphorylasePRO_0000415102Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi143 ↔ 210
Disulfide bondi205 ↔ 266
Disulfide bondi264 ↔ 267

Keywords - PTMi

Disulfide bond

Interactioni

Subunit structurei

Homohexamer. Dimer of a homotrimer.UniRule annotation

Protein-protein interaction databases

STRINGi272557.APE_1885.

Structurei

Secondary structure

1
275
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi5 – 73Combined sources
Beta strandi13 – 186Combined sources
Helixi20 – 223Combined sources
Turni25 – 273Combined sources
Beta strandi29 – 368Combined sources
Beta strandi47 – 526Combined sources
Beta strandi55 – 617Combined sources
Turni62 – 676Combined sources
Helixi71 – 733Combined sources
Helixi76 – 8611Combined sources
Beta strandi90 – 10112Combined sources
Beta strandi114 – 1185Combined sources
Beta strandi130 – 1323Combined sources
Helixi144 – 15714Combined sources
Beta strandi161 – 1633Combined sources
Beta strandi166 – 1705Combined sources
Helixi178 – 1869Combined sources
Beta strandi191 – 1966Combined sources
Helixi197 – 20610Combined sources
Beta strandi210 – 21910Combined sources
Beta strandi223 – 2264Combined sources
Helixi230 – 25122Combined sources
Helixi252 – 2543Combined sources
Helixi261 – 2633Combined sources
Turni265 – 2684Combined sources
Helixi269 – 2713Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1WTAX-ray1.78A1-275[»]
ProteinModelPortaliQ9YAQ8.
SMRiQ9YAQ8. Positions 3-275.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9YAQ8.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni62 – 632Phosphate bindingUniRule annotation
Regioni95 – 962Phosphate bindingUniRule annotation
Regioni219 – 2213Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the PNP/MTAP phosphorylase family. MTAP subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0005.
HOGENOMiHOG000228987.
KOiK00772.
OMAiCEAQLCY.

Family and domain databases

Gene3Di3.40.50.1580. 1 hit.
HAMAPiMF_01963. MTAP.
InterProiIPR010044. MTAP.
IPR000845. Nucleoside_phosphorylase_d.
IPR001369. PNP/MTAP.
IPR018099. Purine_phosphorylase-2_CS.
[Graphical view]
PANTHERiPTHR11904. PTHR11904. 1 hit.
PfamiPF01048. PNP_UDP_1. 1 hit.
[Graphical view]
SUPFAMiSSF53167. SSF53167. 1 hit.
TIGRFAMsiTIGR01694. MTAP. 1 hit.
PROSITEiPS01240. PNP_MTAP_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9YAQ8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MFEITRPPGV RAHVGVIGGS GLYDPGIVEN PVEVKVSTPY GNPSDFIVVG
60 70 80 90 100
DVAGVKVAFL PRHGRGHRIP PHAINYRANI WALKALGVKW VISVSAVGSL
110 120 130 140 150
REDYRPGDFV VPDQFIDMTK NRRHYTFYDG PVTVHVSMAD PFCEDLRQRL
160 170 180 190 200
IDSGRRLGYT VHERGTYVCI EGPRFSTRAE SRVWKDVFKA DIIGMTLVPE
210 220 230 240 250
INLACEAQLC YATLAMVTDY DVWADRPVTA EEVERVMISN VERARRMLYD
260 270
VIPKLAGEPE LERCSCCRAL DTAAI
Length:275
Mass (Da):30,737
Last modified:November 1, 1999 - v1
Checksum:i86CC11C1FB2DB091
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000002 Genomic DNA. Translation: BAA80890.1.
PIRiE72575.
RefSeqiNP_148238.1. NC_000854.2.
WP_010866659.1. NC_000854.2.

Genome annotation databases

EnsemblBacteriaiBAA80890; BAA80890; APE_1885.
GeneIDi1446318.
KEGGiape:APE_1885.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000002 Genomic DNA. Translation: BAA80890.1 .
PIRi E72575.
RefSeqi NP_148238.1. NC_000854.2.
WP_010866659.1. NC_000854.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1WTA X-ray 1.78 A 1-275 [» ]
ProteinModelPortali Q9YAQ8.
SMRi Q9YAQ8. Positions 3-275.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 272557.APE_1885.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAA80890 ; BAA80890 ; APE_1885 .
GeneIDi 1446318.
KEGGi ape:APE_1885.

Phylogenomic databases

eggNOGi COG0005.
HOGENOMi HOG000228987.
KOi K00772.
OMAi CEAQLCY.

Enzyme and pathway databases

UniPathwayi UPA00904 ; UER00873 .
BioCyci APER272557:GJD6-1277-MONOMER.

Miscellaneous databases

EvolutionaryTracei Q9YAQ8.

Family and domain databases

Gene3Di 3.40.50.1580. 1 hit.
HAMAPi MF_01963. MTAP.
InterProi IPR010044. MTAP.
IPR000845. Nucleoside_phosphorylase_d.
IPR001369. PNP/MTAP.
IPR018099. Purine_phosphorylase-2_CS.
[Graphical view ]
PANTHERi PTHR11904. PTHR11904. 1 hit.
Pfami PF01048. PNP_UDP_1. 1 hit.
[Graphical view ]
SUPFAMi SSF53167. SSF53167. 1 hit.
TIGRFAMsi TIGR01694. MTAP. 1 hit.
PROSITEi PS01240. PNP_MTAP_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1.
  2. "Crystal structure of 5'-deoxy-5'-methylthioadenosine from Aeropyrum pernix (R32 form)."
    Tsunoda M., Murakami Y., Nakamura K.T.
    Submitted (NOV-2004) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (1.78 ANGSTROMS) IN COMPLEX WITH PHOSPHATE.

Entry informationi

Entry nameiMTAP_AERPE
AccessioniPrimary (citable) accession number: Q9YAQ8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 25, 2012
Last sequence update: November 1, 1999
Last modified: November 26, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3