Reviewed, UniProtKB/Swiss-Prot Q9YAI1 (NADE_AERPE)

Names and origin

Protein names

Recommended name:
    Probable NH(3)-dependent NAD(+) synthetase
    EC=6.3.1.5

Gene names

Name:	nadE
Ordered Locus Names:	APE_1958.1

Organism

Aeropyrum pernix [Complete proteome] [HAMAP]

Taxonomic identifier

56636 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Desulfurococcales › Desulfurococcaceae › Aeropyrum

Protein attributes

Sequence length	286 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193
Sequence similarities	Belongs to the NAD synthetase family.

Ontologies

Keywords
Ligand	ATP-binding NAD Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 286	286	Probable NH(3)-dependent NAD(+) synthetase HAMAP MF_00193		PRO_0000152220
Regions
	Nucleotide binding	43 – 50	8	ATP By similarity
Sites
	Active site	45	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9YAI1-1 [UniParc]. Last modified July 10, 2007. Version 3. Checksum: 0AAC3C82688B1CE0	FASTA	286	31,771
	10 20 30 40 50 60 MAVNYKVSLD DVVDIDYNGV RQAITQFLRK YLEASGASGY VLGVSGGVDS SLALALAVDA 70 80 90 100 110 120 VGSGRVTALI MPDREVTPER DVEDALRLVR SFGVEHAVID ISPIVMVYIS ALPIFEDEEK 130 140 150 160 170 180 DRVPVGNLRA RIRANILYYY ANKLGKLVLG TGDRSEYLIG YFTKYGDAAC DVAPLTVLYK 190 200 210 220 230 240 SQVRRLAELI GVPRDIAYKP SSPRLWKGHE AEAELGLSYN EIDVILYSRF DLKIPWEEIP 250 260 270 280 RATGLERAKV ERVRLLHEAS SHKRSPPASP DLGEIKKHYK QHAGKK

References

[1]

"Complete genome sequence of an aerobic hyper-thermophilic crenarchaeon, Aeropyrum pernix K1." Kawarabayasi Y., Hino Y., Horikawa H., Yamazaki S., Haikawa Y., Jin-no K., Takahashi M., Sekine M., Baba S., Ankai A., Kosugi H., Hosoyama A., Fukui S., Nagai Y., Nishijima K., Nakazawa H., Takamiya M., Masuda S. , Funahashi T., Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Kubota K., Nakamura Y., Nomura N., Sako Y., Kikuchi H. DNA Res. 6:83-101(1999) [PubMed: 10382966] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000002 Genomic DNA. Translation: BAA80968.2.
PIR	H72497.
RefSeq	NP_148287.2.
3D structure databases
HSSP	HSSP built from PDB template 1IH8 based on UniProtKB P08164.
ModBase	Search...
Genome annotation databases
GeneID	1446370.
GenomeReviews	Gene locus APE_1958.1 in contig BA000002_GR.
KEGG	ape:APE_1958.1.
NMPDR	fig|272557.1.peg.1395.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9YAI1.
Enzyme and pathway databases
BRENDA	6.3.1.5. 256344.
Family and domain databases
HAMAP	MF_00193. [Tree]
InterPro	IPR003694. NAD_synthase. [Graphical view]
Pfam	PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00552. nadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADE_AERPE

Accession

Primary (citable) accession number: Q9YAI1

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	July 10, 2007
Last modified:	January 20, 2009
This is version 49 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)