Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Tyrosine--tRNA ligase

Gene

tyrS

Organism
Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr).UniRule annotation

Catalytic activityi

ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei39TyrosineUniRule annotation1
Binding sitei49ATPCurated1
Binding sitei52ATPCurated1
Binding sitei165TyrosineUniRule annotation1
Binding sitei169TyrosineUniRule annotation1
Binding sitei172TyrosineUniRule annotation1
Binding sitei187TyrosineUniRule annotation1
Binding sitei241ATPCurated1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi6.1.1.1. 171.

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosine--tRNA ligaseUniRule annotation (EC:6.1.1.1UniRule annotation)
Alternative name(s):
Tyrosyl-tRNA synthetaseUniRule annotation
Short name:
TyrRSUniRule annotation
Gene namesi
Name:tyrSUniRule annotation
Ordered Locus Names:APE_2074.1
OrganismiAeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1)
Taxonomic identifieri272557 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiDesulfurococcalesDesulfurococcaceaeAeropyrum
Proteomesi
  • UP000002518 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002402621 – 364Tyrosine--tRNA ligaseAdd BLAST364

Interactioni

Subunit structurei

Homodimer.UniRule annotation1 Publication

Protein-protein interaction databases

STRINGi272557.APE_2074.1.

Structurei

Secondary structure

1364
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi5 – 14Combined sources10
Beta strandi18 – 21Combined sources4
Helixi23 – 32Combined sources10
Beta strandi37 – 42Combined sources6
Helixi51 – 64Combined sources14
Beta strandi68 – 73Combined sources6
Helixi75 – 80Combined sources6
Helixi83 – 86Combined sources4
Helixi88 – 104Combined sources17
Helixi109 – 111Combined sources3
Beta strandi113 – 116Combined sources4
Helixi117 – 121Combined sources5
Helixi124 – 135Combined sources12
Helixi139 – 143Combined sources5
Helixi155 – 157Combined sources3
Helixi161 – 175Combined sources15
Beta strandi179 – 184Combined sources6
Helixi185 – 187Combined sources3
Helixi188 – 198Combined sources11
Turni199 – 202Combined sources4
Beta strandi207 – 211Combined sources5
Beta strandi241 – 243Combined sources3
Helixi244 – 246Combined sources3
Helixi254 – 262Combined sources9
Helixi275 – 282Combined sources8
Turni283 – 286Combined sources4
Beta strandi305 – 308Combined sources4
Helixi309 – 317Combined sources9
Helixi323 – 346Combined sources24
Helixi349 – 360Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2CYAX-ray2.20A1-364[»]
ProteinModelPortaliQ9YA64.
SMRiQ9YA64.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9YA64.

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi238 – 242"KMSKS" region5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. TyrS type 4 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01886. Archaea.
COG0162. LUCA.
HOGENOMiHOG000228238.
KOiK01866.
OMAiFSKLIYP.

Family and domain databases

CDDicd00805. TyrRS_core. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_02009. Tyr_tRNA_synth_type4. 1 hit.
InterProiIPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002307. Tyr-tRNA-ligase.
IPR023678. Tyr-tRNA-ligase_4.
IPR023617. Tyr-tRNA-ligase_arc/euk-type.
[Graphical view]
PfamiPF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PIRSFiPIRSF006588. TyrRS_arch_euk. 1 hit.
PRINTSiPR01040. TRNASYNTHTYR.
TIGRFAMsiTIGR00234. tyrS. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9YA64-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVRVDVEERF NRIARNTVEI VTEEELKGLL ASGARIKGYI GYEPSGVAHI
60 70 80 90 100
GWLVWMYKVK DLVEAGVDFS VLEATWHAYI NDKLGGDMDL IRAAARIVRR
110 120 130 140 150
VMEAAGVPVE RVRFVDAEEL ASDKDYWGLV IRVAKRASLA RVRRALTIMG
160 170 180 190 200
RRAEEAEVDA SKLIYPLMQV SDIFYMDLDI ALGGMDQRKA HMLARDVAEK
210 220 230 240 250
LGRKKPVAIH TPIISSLQGP GRMEASQGEI DDVLAEVKMS KSKPETAVFV
260 270 280 290 300
VDSDDDIRRK IRKAYCPAKQ VQGNPVLEIA RYILFARDGF TLRVDRPAKY
310 320 330 340 350
GGPVEYTSYE ELERDYTDGR LHPLDLKNAV AESLIEVVRP IRGAVLGDPA
360
MKRALEAIEG KVTR
Length:364
Mass (Da):40,765
Last modified:November 1, 1999 - v1
Checksum:i3C9ABE0E3BE7EC6B
GO

Sequence cautioni

The sequence BAA81085 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000002 Genomic DNA. Translation: BAA81085.2. Different initiation.
PIRiE72512.

Genome annotation databases

EnsemblBacteriaiBAA81085; BAA81085; APE_2074.1.
KEGGiape:APE_2074.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000002 Genomic DNA. Translation: BAA81085.2. Different initiation.
PIRiE72512.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2CYAX-ray2.20A1-364[»]
ProteinModelPortaliQ9YA64.
SMRiQ9YA64.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272557.APE_2074.1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA81085; BAA81085; APE_2074.1.
KEGGiape:APE_2074.1.

Phylogenomic databases

eggNOGiarCOG01886. Archaea.
COG0162. LUCA.
HOGENOMiHOG000228238.
KOiK01866.
OMAiFSKLIYP.

Enzyme and pathway databases

BRENDAi6.1.1.1. 171.

Miscellaneous databases

EvolutionaryTraceiQ9YA64.

Family and domain databases

CDDicd00805. TyrRS_core. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_02009. Tyr_tRNA_synth_type4. 1 hit.
InterProiIPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002307. Tyr-tRNA-ligase.
IPR023678. Tyr-tRNA-ligase_4.
IPR023617. Tyr-tRNA-ligase_arc/euk-type.
[Graphical view]
PfamiPF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PIRSFiPIRSF006588. TyrRS_arch_euk. 1 hit.
PRINTSiPR01040. TRNASYNTHTYR.
TIGRFAMsiTIGR00234. tyrS. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSYY_AERPE
AccessioniPrimary (citable) accession number: Q9YA64
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: November 1, 1999
Last modified: November 30, 2016
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.