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Protein

Tyrosine--tRNA ligase

Gene

tyrS

Organism
Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr).UniRule annotation

Catalytic activityi

ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei39 – 391TyrosineUniRule annotation
Binding sitei49 – 491ATPCurated
Binding sitei52 – 521ATPCurated
Binding sitei165 – 1651TyrosineUniRule annotation
Binding sitei169 – 1691TyrosineUniRule annotation
Binding sitei172 – 1721TyrosineUniRule annotation
Binding sitei187 – 1871TyrosineUniRule annotation
Binding sitei241 – 2411ATPCurated

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciAPER272557:GJD6-1400-MONOMER.
BRENDAi6.1.1.1. 171.

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosine--tRNA ligaseUniRule annotation (EC:6.1.1.1UniRule annotation)
Alternative name(s):
Tyrosyl-tRNA synthetaseUniRule annotation
Short name:
TyrRSUniRule annotation
Gene namesi
Name:tyrSUniRule annotation
Ordered Locus Names:APE_2074.1
OrganismiAeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1)
Taxonomic identifieri272557 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiDesulfurococcalesDesulfurococcaceaeAeropyrum
Proteomesi
  • UP000002518 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 364364Tyrosine--tRNA ligasePRO_0000240262Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation1 Publication

Protein-protein interaction databases

STRINGi272557.APE_2074.1.

Structurei

Secondary structure

1
364
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi5 – 1410Combined sources
Beta strandi18 – 214Combined sources
Helixi23 – 3210Combined sources
Beta strandi37 – 426Combined sources
Helixi51 – 6414Combined sources
Beta strandi68 – 736Combined sources
Helixi75 – 806Combined sources
Helixi83 – 864Combined sources
Helixi88 – 10417Combined sources
Helixi109 – 1113Combined sources
Beta strandi113 – 1164Combined sources
Helixi117 – 1215Combined sources
Helixi124 – 13512Combined sources
Helixi139 – 1435Combined sources
Helixi155 – 1573Combined sources
Helixi161 – 17515Combined sources
Beta strandi179 – 1846Combined sources
Helixi185 – 1873Combined sources
Helixi188 – 19811Combined sources
Turni199 – 2024Combined sources
Beta strandi207 – 2115Combined sources
Beta strandi241 – 2433Combined sources
Helixi244 – 2463Combined sources
Helixi254 – 2629Combined sources
Helixi275 – 2828Combined sources
Turni283 – 2864Combined sources
Beta strandi305 – 3084Combined sources
Helixi309 – 3179Combined sources
Helixi323 – 34624Combined sources
Helixi349 – 36012Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2CYAX-ray2.20A1-364[»]
ProteinModelPortaliQ9YA64.
SMRiQ9YA64. Positions 4-361.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9YA64.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi238 – 2425"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. TyrS type 4 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01886. Archaea.
COG0162. LUCA.
HOGENOMiHOG000228238.
KOiK01866.
OMAiFSKLIYP.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_02009. Tyr_tRNA_synth_type4. 1 hit.
InterProiIPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002307. Tyr-tRNA-ligase.
IPR023678. Tyr-tRNA-ligase_4.
IPR023617. Tyr-tRNA-ligase_arc/euk-type.
[Graphical view]
PfamiPF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PIRSFiPIRSF006588. TyrRS_arch_euk. 1 hit.
PRINTSiPR01040. TRNASYNTHTYR.
TIGRFAMsiTIGR00234. tyrS. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9YA64-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVRVDVEERF NRIARNTVEI VTEEELKGLL ASGARIKGYI GYEPSGVAHI
60 70 80 90 100
GWLVWMYKVK DLVEAGVDFS VLEATWHAYI NDKLGGDMDL IRAAARIVRR
110 120 130 140 150
VMEAAGVPVE RVRFVDAEEL ASDKDYWGLV IRVAKRASLA RVRRALTIMG
160 170 180 190 200
RRAEEAEVDA SKLIYPLMQV SDIFYMDLDI ALGGMDQRKA HMLARDVAEK
210 220 230 240 250
LGRKKPVAIH TPIISSLQGP GRMEASQGEI DDVLAEVKMS KSKPETAVFV
260 270 280 290 300
VDSDDDIRRK IRKAYCPAKQ VQGNPVLEIA RYILFARDGF TLRVDRPAKY
310 320 330 340 350
GGPVEYTSYE ELERDYTDGR LHPLDLKNAV AESLIEVVRP IRGAVLGDPA
360
MKRALEAIEG KVTR
Length:364
Mass (Da):40,765
Last modified:November 1, 1999 - v1
Checksum:i3C9ABE0E3BE7EC6B
GO

Sequence cautioni

The sequence BAA81085 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000002 Genomic DNA. Translation: BAA81085.2. Different initiation.
PIRiE72512.

Genome annotation databases

EnsemblBacteriaiBAA81085; BAA81085; APE_2074.1.
KEGGiape:APE_2074.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000002 Genomic DNA. Translation: BAA81085.2. Different initiation.
PIRiE72512.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2CYAX-ray2.20A1-364[»]
ProteinModelPortaliQ9YA64.
SMRiQ9YA64. Positions 4-361.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272557.APE_2074.1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA81085; BAA81085; APE_2074.1.
KEGGiape:APE_2074.1.

Phylogenomic databases

eggNOGiarCOG01886. Archaea.
COG0162. LUCA.
HOGENOMiHOG000228238.
KOiK01866.
OMAiFSKLIYP.

Enzyme and pathway databases

BioCyciAPER272557:GJD6-1400-MONOMER.
BRENDAi6.1.1.1. 171.

Miscellaneous databases

EvolutionaryTraceiQ9YA64.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_02009. Tyr_tRNA_synth_type4. 1 hit.
InterProiIPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002307. Tyr-tRNA-ligase.
IPR023678. Tyr-tRNA-ligase_4.
IPR023617. Tyr-tRNA-ligase_arc/euk-type.
[Graphical view]
PfamiPF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PIRSFiPIRSF006588. TyrRS_arch_euk. 1 hit.
PRINTSiPR01040. TRNASYNTHTYR.
TIGRFAMsiTIGR00234. tyrS. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSYY_AERPE
AccessioniPrimary (citable) accession number: Q9YA64
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: November 1, 1999
Last modified: September 7, 2016
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.