Reviewed, UniProtKB/Swiss-Prot Q9Y9X3 (SYA_AERPE)

Names and origin

Protein names

Recommended name:
    Alanyl-tRNA synthetase
    EC=6.1.1.7
Alternative name(s):
    Alanine--tRNA ligase
      Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	APE_2166

Organism

Aeropyrum pernix [Complete proteome] [HAMAP]

Taxonomic identifier

56636 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Desulfurococcales › Desulfurococcaceae › Aeropyrum

Protein attributes

Sequence length	900 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala). HAMAP MF_00036
Subcellular location	Cytoplasm. HAMAP MF_00036
Domain	The C-terminal C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding RNA-binding tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP alanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 900	900	Alanyl-tRNA synthetase HAMAP MF_00036		PRO_0000075259

Sequences

	Sequence		Length	Mass (Da)
	Q9Y9X3-1 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: 4602BC9B249C4C04	FASTA	900	102,333
	10 20 30 40 50 60 MGFVRKEGAR CREAFWTLNP EFSEPQDTPC VEYWFDKVKS VSGMSVGEAR EAFLSFFEKH 70 80 90 100 110 120 GHTRVPPRPV VARWREDLYL TIASIVVFQP HVTSGLVPPP ANPLVISQPS IRLEDIDNVG 130 140 150 160 170 180 ITIGRHLTSF EMAAHHAFNY PDRQVYWKEE TVRLAFEFFT QVLGIPPELI VFKESWWEGG 190 200 210 220 230 240 GNAGPSFEVA VGGLELATLV FMKYRVVDGR YEEIPLKIVD TGYGVERLAW FTQKTPTAFH 250 260 270 280 290 300 AIYGSLVDDF RRMLGVEKPD ENVMWAAFRV AGFLDPEDPE SLRNYYQTVA SLAGGDVETV 310 320 330 340 350 360 RSILTREARL YSVLDHTKTI ALMLGDGIVP SNSGEGYLAR LVVRRALRQL SLLNAEVPLV 370 380 390 400 410 420 DLVERQARFW MRDFPQLKEN LDYILDAVSL EEERFRDVLR KARSIVEREL KRKKRLGVDD 430 440 450 460 470 480 LIRLYDSMGV PPEIAAEIAA SRGAPVEVPH NFYSLVAARH RGPEKIRGYG FDETGLPRDV 490 500 510 520 530 540 EEWARRFGET RRVFHEDPYA KAWKARVLGV KGRYLVADST IFYPTGGGQI HDTGVIRVNN 550 560 570 580 590 600 QQYRIIDVQK VGDAIVHVAE REIAAEPGDE VWMEIDWERR YSIMRHHTVT HVLIAAARRV 610 620 630 640 650 660 LGRHAWQAGA EKTEEKGRLD ITHHRPLTRD DIEKLENVVN QVIRERRRVW EDMVDKNEAE 670 680 690 700 710 720 EKYGFTIYQG GVPMEKRLRL VFVEDWDVEA CFGTHVRNTG EIGGFKIISY SRIQDGVVRL 730 740 750 760 770 780 EYVAGDRVAI YARELEERLA RIGDAVKAPR GQEEARVKGL IASLEQARED LKRYRDYWVK 790 800 810 820 830 840 TIEEAYISRA RRVNGVKVLA VESLEKDRRT VQEILRKLTS RHEDLIAALV VENEGNTQVE 850 860 870 880 890 900 IAAGPKAAEK VDLGALVKIV IKKVGGRGGG RGSYASLRVE GRLSADKVEE LLADALENVL

References

[1]

"Complete genome sequence of an aerobic hyper-thermophilic crenarchaeon, Aeropyrum pernix K1." Kawarabayasi Y., Hino Y., Horikawa H., Yamazaki S., Haikawa Y., Jin-no K., Takahashi M., Sekine M., Baba S., Ankai A., Kosugi H., Hosoyama A., Fukui S., Nagai Y., Nishijima K., Nakazawa H., Takamiya M., Masuda S. , Funahashi T., Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Kubota K., Nakamura Y., Nomura N., Sako Y., Kikuchi H. DNA Res. 6:83-101(1999) [PubMed: 10382966] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000002 Genomic DNA. Translation: BAA81177.1.
PIR	A72524.
RefSeq	NP_148431.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1445236.
GenomeReviews	Gene locus APE_2166 in contig BA000002_GR.
KEGG	ape:APE_2166.
NMPDR	fig|272557.1.peg.1539.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9Y9X3.
OMA	TLMFTNS.
Enzyme and pathway databases
BRENDA	6.1.1.7. 256344.
Family and domain databases
HAMAP	MF_00036. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR018165. Ala-tRNA-synth_IIc_cons-reg. IPR018164. Ala-tRNA-synth_IIc_N. IPR003156. Pesterase_DHHA1. IPR012947. tRNA_SAD. [Graphical view]
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
TIGRFAMs	TIGR00344. alaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYA_AERPE

Accession

Primary (citable) accession number: Q9Y9X3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1999
Last modified:	November 3, 2009
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)