Reviewed, UniProtKB/Swiss-Prot Q9Y9I6 (SYFA_AERPE)

Names and origin

Protein names

Recommended name:
    Phenylalanyl-tRNA synthetase alpha chain
    EC=6.1.1.20
Alternative name(s):
    Phenylalanine--tRNA ligase alpha chain
      Short name=PheRS

Gene names

Name:	pheS
Ordered Locus Names:	APE_2302.1

Organism

Aeropyrum pernix [Complete proteome] [HAMAP]

Taxonomic identifier

56636 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Desulfurococcales › Desulfurococcaceae › Aeropyrum

Protein attributes

Sequence length	488 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe). HAMAP MF_00282
Cofactor	Binds 2 magnesium ions per tetramer By similarity.
Subunit structure	Tetramer of two alpha and two beta chains By similarity.
Subcellular location	Cytoplasm. HAMAP MF_00282
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 2 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phenylalanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP phenylalanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 488	488	Phenylalanyl-tRNA synthetase alpha chain HAMAP MF_00282		PRO_0000126806

Sequences

	Sequence		Length	Mass (Da)
	Q9Y9I6-1 [UniParc]. Last modified December 8, 2000. Version 2. Checksum: D2E284563C89457B	FASTA	488	55,084
	10 20 30 40 50 60 MEVTLSQGEY RLLKLMAERG FREGTLEEAS KILGVDKSSL ASLSNLLAEK GVVEVEERVE 70 80 90 100 110 120 EHYVLTERGR DALERGLPEE KLVLFLAGRG GEASVEEVRR ALGEEAGIAL GQAARKGLVV 130 140 150 160 170 180 IAGGVVRLAV DQAEALKTIT PLKKLLENVA SGSKPTVGDE LLREALSRGL IRREARRSIV 190 200 210 220 230 240 LRLKVNPAEA LARARVEAAV LTRDMLKSGE WRRLRFKPYN VKAEPPRVLP ARRHFLAEFI 250 260 270 280 290 300 ERLRDILREL GFREVRGPLV ELELFNFDVL FQAQDHPARE IHDSLWIKSP RRGDLSGYSD 310 320 330 340 350 360 LVERVASVHE RGWKYRWSPE VASRYILRSQ TTAVSARILA TRPNPPARFF TVGKVFRSDA 370 380 390 400 410 420 VGPTRLPEFH QLDGIEGDEG YTFRDLLGRL DEIASMLGLK LKFKPAYFPF TEPSVEGYVK 430 440 450 460 470 480 LPNGRWLELF GAGMFRPEVL EAVGVDYPVG AWGFGIERLA MAFYGVSDIR KLYTRNVDEV REMRVRWL

References

[1]

"Complete genome sequence of an aerobic hyper-thermophilic crenarchaeon, Aeropyrum pernix K1." Kawarabayasi Y., Hino Y., Horikawa H., Yamazaki S., Haikawa Y., Jin-no K., Takahashi M., Sekine M., Baba S., Ankai A., Kosugi H., Hosoyama A., Fukui S., Nagai Y., Nishijima K., Nakazawa H., Takamiya M., Masuda S. , Funahashi T., Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Kubota K., Nakamura Y., Nomura N., Sako Y., Kikuchi H. DNA Res. 6:83-101(1999) [PubMed: 10382966] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000002 Genomic DNA. Translation: BAA81314.2.
PIR	B72457.
RefSeq	NP_148525.2.
3D structure databases
HSSP	HSSP built from PDB template 1JJC based on UniProtKB P27001.
ModBase	Search...
Genome annotation databases
GeneID	1445333.
GenomeReviews	Gene locus APE_2302.1 in contig BA000002_GR.
KEGG	ape:APE_2302.1.
NMPDR	fig|272557.1.peg.1633.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9Y9I6.
OMA	FHQLDGI.
Enzyme and pathway databases
BRENDA	6.1.1.20. 256344.
Family and domain databases
HAMAP	MF_00282. [Tree]
InterPro	IPR006195. aa-tRNA-synth_II_cons-reg. IPR004529. Phe-tRNA-synth_IIc_asu. IPR002319. Phenylalanyl-tRNA_Synthase_acu. [Graphical view]
PANTHER	PTHR11538. tRNA-synt_2d. 1 hit.
Pfam	PF01409. tRNA-synt_2d. 1 hit. [Graphical view]
ProDom	PD006327. Wing_hlx_tran_reg. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00468. pheS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYFA_AERPE

Accession

Primary (citable) accession number: Q9Y9I6

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 8, 2000
Last sequence update:	December 8, 2000
Last modified:	November 3, 2009
This is version 63 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)