Reviewed, UniProtKB/Swiss-Prot Q9Y9G0 (SYP_AERPE)

Names and origin

Protein names

Recommended name:
    Prolyl-tRNA synthetase
    EC=6.1.1.15
Alternative name(s):
    Proline--tRNA ligase
      Short name=ProRS

Gene names

Name:	proS
Ordered Locus Names:	APE_2328

Organism

Aeropyrum pernix [Complete proteome] [HAMAP]

Taxonomic identifier

56636 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Desulfurococcales › Desulfurococcaceae › Aeropyrum

Protein attributes

Sequence length	485 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro) By similarity.
Catalytic activity	ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro). HAMAP MF_01571
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Domain	Consists of three domains: the N-terminal catalytic domain, the anticodon-binding domain and the C-terminal extension By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	prolyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP proline-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 485	485	Prolyl-tRNA synthetase HAMAP MF_01571		PRO_0000249155

Sequences

	Sequence		Length	Mass (Da)
	Q9Y9G0-1 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: 8A451465E1C26CC5	FASTA	485	56,185
	10 20 30 40 50 60 MLGPPSREKW SSDFPRWFDW VIETAEVYDY GRYPVKGMGV WMPYGFQIRR RVLEVVRGLL 70 80 90 100 110 120 DSTGHEEVLF PLLIPEHLLR RESEHIRGFE GEVYWVTHGG REELDVKLAL RPTSETSITY 130 140 150 160 170 180 METFWIKSYR QLPKKYYQVV SIFRYETKAT RPMIRLREVT TFKEAHTVHE SFEDAERQVL 190 200 210 220 230 240 EAIEVYKAIF DRLLIPYVIS KRPEWDKFAG ALYTIAFDTI MPDGRALQIG TVHHLGQSFT 250 260 270 280 290 300 RAFDFRIQMR DERLDHPWQT SYGVSDRVVA SLIAVHGDDR GLVIPPSVAP IQVVVIPITP 310 320 330 340 350 360 GDEEKRGKVL TYTAKAAEAL EKAGLRVHVD DREWERPGAK FYYWEAKGVP IRVEIGLREA 370 380 390 400 410 420 EQDTLTIARR DTLEKTEVPL GEAGNRIREL MAQIESSMRE RAKSFFGERL LRTESLEEAR 430 440 450 460 470 480 DWVEGRRGIA EIPWCGRESC GLEMEERVNG KVLGTPWPEE PVEEGKRCPL CGRPAVAWIR LAKTY

References

[1]

"Complete genome sequence of an aerobic hyper-thermophilic crenarchaeon, Aeropyrum pernix K1." Kawarabayasi Y., Hino Y., Horikawa H., Yamazaki S., Haikawa Y., Jin-no K., Takahashi M., Sekine M., Baba S., Ankai A., Kosugi H., Hosoyama A., Fukui S., Nagai Y., Nishijima K., Nakazawa H., Takamiya M., Masuda S. , Funahashi T., Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Kubota K., Nakamura Y., Nomura N., Sako Y., Kikuchi H. DNA Res. 6:83-101(1999) [PubMed: 10382966] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000002 Genomic DNA. Translation: BAA81340.1.
PIR	D72460.
RefSeq	NP_148542.1.
3D structure databases
HSSP	HSSP built from PDB template 1NJ2 based on UniProtKB O26708.
ModBase	Search...
Genome annotation databases
GeneID	1445351.
GenomeReviews	Gene locus APE_2328 in contig BA000002_GR.
KEGG	ape:APE_2328.
NMPDR	fig|272557.1.peg.1650.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9Y9G0.
OMA	VCGREAK.
Enzyme and pathway databases
BRENDA	6.1.1.15. 256344.
Family and domain databases
HAMAP	MF_01571. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-reg. IPR006195. aa-tRNA-synth_II_cons-reg. IPR004154. Anticodon_bd. IPR002316. Pro-tRNA-synth_IIa_cons-reg. IPR004499. Pro-tRNA-synth_IIa_pro-type. IPR016061. Pro-tRNA_synth_II_C. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit. G3DSA:3.30.110.30. Pro-tRNA-synth_II_C_arc/euk. 1 hit.
PANTHER	PTHR11451:SF6. ProS_fam_I. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF09180. ProRS-C_1. 1 hit. PF00587. tRNA-synt_2b. 1 hit. [Graphical view]
PRINTS	PR01046. TRNASYNTHPRO.
TIGRFAMs	TIGR00408. proS_fam_I. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYP_AERPE

Accession

Primary (citable) accession number: Q9Y9G0

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	November 1, 1999
Last modified:	November 3, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)