Reviewed, UniProtKB/Swiss-Prot Q9Y9E6 (LPLA_AERPE)

Names and origin

Protein names

Recommended name:
    Putative lipoate-protein ligase A
      Short name=Lipoate--protein ligase
    EC=2.7.7.63

Gene names

Name:	lplA
Synonyms:	lipB
Ordered Locus Names:	APE_2341

Organism

Aeropyrum pernix [Complete proteome] [HAMAP]

Taxonomic identifier

56636 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Desulfurococcales › Desulfurococcaceae › Aeropyrum

Protein attributes

Sequence length	264 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes both the ATP-dependent activation of exogenously supplied lipoate to lipoyl-AMP and the transfer of the activated lipoyl onto the lipoyl domains of lipoate-dependent enzymes By similarity.
Catalytic activity	ATP + lipoate = diphosphate + lipoyl-AMP. Lipoyl-AMP + protein = protein N(6)-(lipoyl)lysine + AMP.
Pathway	Protein modification; protein lipoylation via exogenous pathway; protein N(6)-(lipoyl)lysine from lipoic acid: step 1/2. Protein modification; protein lipoylation via exogenous pathway; protein N(6)-(lipoyl)lysine from lipoic acid: step 2/2.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Miscellaneous	In the transfer reaction, the free carboxyl group of lipoic acid is attached via an amide linkage to the epsilon-amino group of a specific lysine residue of lipoyl domains of lipoate-dependent enzymes By similarity.
Sequence similarities	Belongs to the lplA family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein modification process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW transferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 264	264	Putative lipoate-protein ligase A		PRO_0000062902
Regions
	Nucleotide binding	73 – 76	4	ATP By similarity
Sites
	Binding site	68	1	ATP By similarity
	Binding site	130	1	ATP By similarity
	Binding site	130	1	Lipoate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9Y9E6-1 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: 1814419916955924	FASTA	264	28,890
	10 20 30 40 50 60 MILKTTGGSP HFNIALEEAL LEESAEHGIA IARLWVNPDS IIVGYTSDVG REVNIEQARA 70 80 90 100 110 120 EGVPVVRRIS GGGAVFHDLG NMNVSVYIPR RLGVDEAYAL VTSIILKTLH RLGIEGRVEN 130 140 150 160 170 180 GNDVAVGPWK VSGSAAAIRA RATLAHATLL LTTDPSRIRR LVIPQLHRVE RGEVTPVKYN 190 200 210 220 230 240 PNSLERITGE RMEVWQAARL LEESVKHALG EPENVGRDIL QEAVIRAREL CKTKYSQKGF 250 260 WSPLGLGACR EPQVAPMTSP SYVL

References

[1]

"Complete genome sequence of an aerobic hyper-thermophilic crenarchaeon, Aeropyrum pernix K1." Kawarabayasi Y., Hino Y., Horikawa H., Yamazaki S., Haikawa Y., Jin-no K., Takahashi M., Sekine M., Baba S., Ankai A., Kosugi H., Hosoyama A., Fukui S., Nagai Y., Nishijima K., Nakazawa H., Takamiya M., Masuda S. , Funahashi T., Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Kubota K., Nakamura Y., Nomura N., Sako Y., Kikuchi H. DNA Res. 6:83-101(1999) [PubMed: 10382966] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000002 Genomic DNA. Translation: BAA81354.1.
PIR	B72462.
RefSeq	NP_148553.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1445362.
GenomeReviews	Gene locus APE_2341 in contig BA000002_GR.
KEGG	ape:APE_2341.
NMPDR	fig|272557.1.peg.1661.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9Y9E6.
OMA	Q9Y9E6. NSLERIT.
Enzyme and pathway databases
BRENDA	2.7.7.63. 256344.
Family and domain databases
InterPro	IPR004143. BPL_LipA_LipB. [Graphical view]
Pfam	PF03099. BPL_LipA_LipB. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LPLA_AERPE

Accession

Primary (citable) accession number: Q9Y9E6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1999
Last modified:	June 16, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)