Reviewed, UniProtKB/Swiss-Prot Q9Y9D8 (PYRE_AERPE)

Names and origin

Protein names

Recommended name:
    Orotate phosphoribosyltransferase
      Short name=OPRT
      Short name=OPRTase
    EC=2.4.2.10

Gene names

Name:	pyrE
Ordered Locus Names:	APE_2349.1

Organism

Aeropyrum pernix [Complete proteome] [HAMAP]

Taxonomic identifier

56636 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Desulfurococcales › Desulfurococcaceae › Aeropyrum

Protein attributes

Sequence length	186 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) By similarity. HAMAP MF_01208
Catalytic activity	Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP MF_01208
Cofactor	Magnesium By similarity. HAMAP MF_01208
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2. HAMAP MF_01208
Subunit structure	Homodimer By similarity. HAMAP MF_01208
Sequence similarities	Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Ligand	Magnesium
Molecular function	Glycosyltransferase Transferase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	magnesium ion binding Inferred from electronic annotation. Source: HAMAP orotate phosphoribosyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 186

186

Orotate phosphoribosyltransferase HAMAP MF_01208

PRO_0000110776

Regions

Region

121 – 129

9

5-phosphoribose 1-diphosphate binding By similarity

Sites

Binding site

96

1

5-phosphoribose 1-diphosphate; shared with dimeric partner By similarity

Binding site

100

1

5-phosphoribose 1-diphosphate; shared with dimeric partner By similarity

Binding site

102

1

5-phosphoribose 1-diphosphate; shared with dimeric partner By similarity

Binding site

125

1

Orotate By similarity

Binding site

153

1

Orotate By similarity

Secondary structure

1

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186

Helix Strand Turn

Details...

Helix

10 – 16

7

Beta strand

19 – 26

8

Beta strand

32 – 37

6

Helix

39 – 42

4

Helix

46 – 66

21

Beta strand

68 – 73

6

Turn

74 – 77

4

Helix

78 – 88

11

Beta strand

92 – 95

4

Beta strand

114 – 127

14

Helix

128 – 139

12

Beta strand

143 – 152

10

Helix

157 – 162

6

Turn

163 – 165

3

Beta strand

167 – 173

7

Helix

174 – 180

7

Sequences

	Sequence		Length	Mass (Da)
	Q9Y9D8-1 [UniParc]. Last modified July 10, 2007. Version 2. Checksum: A05A8636B3A22B91	FASTA	186	19,638
	10 20 30 40 50 60 MSHEELAEVL AKVLKKRGAV LRGDFVLSSG RRSSVYIDMR RLLGDESSYS VALDLLLEVG 70 80 90 100 110 120 GQDLARSSAV IGVATGGLPW AAMLALRLSK PLGYVRPERK GHGTLSQVEG DPPKGRVVVV 130 140 150 160 170 180 DDVATTGTSI AKSIEVLRSN GYTVGTALVL VDRGEGAGEL LARMGVRLVS VATLKTILEK LGWGGE

References

[1]

"Complete genome sequence of an aerobic hyper-thermophilic crenarchaeon, Aeropyrum pernix K1." Kawarabayasi Y., Hino Y., Horikawa H., Yamazaki S., Haikawa Y., Jin-no K., Takahashi M., Sekine M., Baba S., Ankai A., Kosugi H., Hosoyama A., Fukui S., Nagai Y., Nishijima K., Nakazawa H., Takamiya M., Masuda S. , Funahashi T., Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Kubota K., Nakamura Y., Nomura N., Sako Y., Kikuchi H. DNA Res. 6:83-101(1999) [PubMed: 10382966] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

BA000002 Genomic DNA. Translation: BAA81362.2.

PIR

B72463.

RefSeq

NP_148558.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2YZK	X-ray	1.80	A/B/C/D	10-186	[»]

ModBase

Search...

Genome annotation databases

GeneID

1445367.

GenomeReviews

Gene locus APE_2349.1 in contig BA000002_GR.

KEGG

ape:APE_2349.1.

NMPDR

fig|272557.1.peg.1666.

Organism-specific databases

CMR

Search...

Phylogenomic databases

HOGENOM

HBG404341.

Enzyme and pathway databases

BioCyc

APER272557:APE2349-MONOMER.

BRENDA

2.4.2.10. 256344.

Family and domain databases

HAMAP

MF_01208_A. PyrE_A.
[Tree]

InterPro

IPR004467. Or_phspho_trans.
IPR000836. PRibTrfase.
[Graphical view]

Pfam

PF00156. Pribosyltran. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00336. pyrE. 1 hit.

PROSITE

PS00103. PUR_PYR_PR_TRANSFER. False negative.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

PYRE_AERPE

Accession

Primary (citable) accession number: Q9Y9D8

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 6, 2002
Last sequence update:	July 10, 2007
Last modified:	February 9, 2010
This is version 52 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)