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Protein

Orotate phosphoribosyltransferase

Gene

pyrE

Organism
Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP).UniRule annotation

Catalytic activityi

Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: UMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes UMP from orotate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Orotate phosphoribosyltransferase (pyrE)
  2. Orotidine 5'-phosphate decarboxylase (pyrF)
This subpathway is part of the pathway UMP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes UMP from orotate, the pathway UMP biosynthesis via de novo pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei96 – 9615-phosphoribose 1-diphosphate; shared with dimeric partnerUniRule annotation
Binding sitei100 – 10015-phosphoribose 1-diphosphate; shared with dimeric partnerUniRule annotation
Binding sitei102 – 10215-phosphoribose 1-diphosphate; shared with dimeric partnerUniRule annotation
Binding sitei125 – 1251OrotateUniRule annotation
Binding sitei153 – 1531OrotateUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Pyrimidine biosynthesis

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BioCyciAPER272557:GJD6-1586-MONOMER.
UniPathwayiUPA00070; UER00119.

Names & Taxonomyi

Protein namesi
Recommended name:
Orotate phosphoribosyltransferaseUniRule annotation (EC:2.4.2.10UniRule annotation)
Short name:
OPRTUniRule annotation
Short name:
OPRTaseUniRule annotation
Gene namesi
Name:pyrEUniRule annotation
Ordered Locus Names:APE_2349.1
OrganismiAeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1)
Taxonomic identifieri272557 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiDesulfurococcalesDesulfurococcaceaeAeropyrum
Proteomesi
  • UP000002518 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 186186Orotate phosphoribosyltransferasePRO_0000110776Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi272557.APE_2349.1.

Structurei

Secondary structure

1
186
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi10 – 167Combined sources
Beta strandi19 – 268Combined sources
Beta strandi32 – 376Combined sources
Helixi39 – 424Combined sources
Helixi46 – 6621Combined sources
Beta strandi68 – 736Combined sources
Turni74 – 774Combined sources
Helixi78 – 8811Combined sources
Beta strandi92 – 954Combined sources
Beta strandi114 – 12714Combined sources
Helixi128 – 13912Combined sources
Beta strandi143 – 15210Combined sources
Helixi157 – 1626Combined sources
Turni163 – 1653Combined sources
Beta strandi167 – 1737Combined sources
Helixi174 – 1807Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2YZKX-ray1.80A/B/C/D10-186[»]
ProteinModelPortaliQ9Y9D8.
SMRiQ9Y9D8. Positions 9-185.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9Y9D8.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni121 – 12995-phosphoribose 1-diphosphate bindingUniRule annotation

Sequence similaritiesi

Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00029. Archaea.
COG0461. LUCA.
HOGENOMiHOG000037976.
KOiK00762.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
HAMAPiMF_01208. PyrE.
InterProiIPR023031. OPRT.
IPR004467. Or_phspho_trans_dom.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR00336. pyrE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9Y9D8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSHEELAEVL AKVLKKRGAV LRGDFVLSSG RRSSVYIDMR RLLGDESSYS
60 70 80 90 100
VALDLLLEVG GQDLARSSAV IGVATGGLPW AAMLALRLSK PLGYVRPERK
110 120 130 140 150
GHGTLSQVEG DPPKGRVVVV DDVATTGTSI AKSIEVLRSN GYTVGTALVL
160 170 180
VDRGEGAGEL LARMGVRLVS VATLKTILEK LGWGGE
Length:186
Mass (Da):19,638
Last modified:July 10, 2007 - v2
Checksum:iA05A8636B3A22B91
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000002 Genomic DNA. Translation: BAA81362.2.
PIRiB72463.

Genome annotation databases

EnsemblBacteriaiBAA81362; BAA81362; APE_2349.1.
KEGGiape:APE_2349.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000002 Genomic DNA. Translation: BAA81362.2.
PIRiB72463.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2YZKX-ray1.80A/B/C/D10-186[»]
ProteinModelPortaliQ9Y9D8.
SMRiQ9Y9D8. Positions 9-185.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272557.APE_2349.1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA81362; BAA81362; APE_2349.1.
KEGGiape:APE_2349.1.

Phylogenomic databases

eggNOGiarCOG00029. Archaea.
COG0461. LUCA.
HOGENOMiHOG000037976.
KOiK00762.

Enzyme and pathway databases

UniPathwayiUPA00070; UER00119.
BioCyciAPER272557:GJD6-1586-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ9Y9D8.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
HAMAPiMF_01208. PyrE.
InterProiIPR023031. OPRT.
IPR004467. Or_phspho_trans_dom.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR00336. pyrE. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1.

Entry informationi

Entry nameiPYRE_AERPE
AccessioniPrimary (citable) accession number: Q9Y9D8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 6, 2002
Last sequence update: July 10, 2007
Last modified: November 11, 2015
This is version 86 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.