Q9Y935 (AMPA_AERPE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable cytosol aminopeptidase
EC=3.4.11.1

Alternative name(s):
Leucine aminopeptidase
Short name=LAP
EC=3.4.11.10
Leucyl aminopeptidase

Gene names

Name:	pepA
Ordered Locus Names:	APE_2450.1

Organism

Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1) [Complete proteome] [HAMAP]

Taxonomic identifier

272557 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Desulfurococcales › Desulfurococcaceae › Aeropyrum

Protein attributes

Sequence length	492 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides By similarity. HAMAP MF_00181
Catalytic activity	Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low. HAMAP MF_00181 Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.
Cofactor	Binds 2 manganese ions per subunit By similarity. HAMAP MF_00181
Subcellular location	Cytoplasm By similarity HAMAP MF_00181.
Sequence similarities	Belongs to the peptidase M17 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Manganese Metal-binding
Molecular function	Aminopeptidase Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: InterPro metalloexopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 492	492	Probable cytosol aminopeptidase HAMAP MF_00181		PRO_0000165822
Sites
	Active site	260	1	Potential
	Active site	334	1	Potential
	Metal binding	248	1	Manganese 2 By similarity
	Metal binding	253	1	Manganese 1 By similarity
	Metal binding	253	1	Manganese 2 By similarity
	Metal binding	271	1	Manganese 2 By similarity
	Metal binding	330	1	Manganese 1 By similarity
	Metal binding	332	1	Manganese 1 By similarity
	Metal binding	332	1	Manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9Y935 [UniParc]. Last modified July 10, 2007. Version 2. Checksum: BA7A346C04D16260	FASTA	492	52,387
	10 20 30 40 50 60 MVLYTRPPRL SVSRETFFGR QTPVVIPVFK EGDSIKLPEG LPQDLESLLK ESYNSKAVSP 70 80 90 100 110 120 EPGSVAKLPY RGGLVVTAGC GAPGDLEGVR RGFAAASRQV VDSFEEAQLY LVGLDTVSST 130 140 150 160 170 180 EAVIGALLGA YRLEEFKNTR KRKLQQLWVY GGEPRLDYAQ AVAEGVYLAR DIANAPPHRL 190 200 210 220 230 240 PPAKLAAAVE DLFSKFDNVD VEVFSYDRLL EEGFGGIVSV GMGSEEKPRL IIIKYRGGAG 250 260 270 280 290 300 EPIALVGKAV VFDSGGINLK PSQGMTLMRA DKAGGAAVVG AMWTAARLGI KASIIGLIPA 310 320 330 340 350 360 VINVPSGSSY LPSDVIRMWD GTMVEITNTD AEGRLILADA ISYAAKQLGA KTVIDLATLT 370 380 390 400 410 420 GAIVVALGPL IAGLFTRSDG LARAFEEASR TTGEKIWRMP MEDEYAKSLT QPAQAGEIVN 430 440 450 460 470 480 AAQRYGGAIF GALFLERFVH GKEFAHLDIA GPGIAFEAGS LAPPYWPEKG MAPGYGVRLL 490 IEVLSRMARG GG

References

[1]

"Complete genome sequence of an aerobic hyper-thermophilic crenarchaeon, Aeropyrum pernix K1." Kawarabayasi Y., Hino Y., Horikawa H., Yamazaki S., Haikawa Y., Jin-no K., Takahashi M., Sekine M., Baba S., Ankai A., Kosugi H., Hosoyama A., Fukui S., Nagai Y., Nishijima K., Nakazawa H., Takamiya M., Masuda S. , Funahashi T., Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Kubota K., Nakamura Y., Nomura N., Sako Y., Kikuchi H. DNA Res. 6:83-101(1999) [PubMed: 10382966] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000002 Genomic DNA. Translation: BAA81465.2.
PIR	A72476.
RefSeq	NP_148619.2. NC_000854.2.
3D structure databases
ProteinModelPortal	Q9Y935.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1445428.
GenomeReviews	Gene locus APE_2450.1 in contig BA000002_GR.
KEGG	ape:APE_2450.1.
NMPDR	fig|272557.1.peg.1727.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG742580.
Enzyme and pathway databases
BioCyc	APER272557:APE2450-MONOMER.
Family and domain databases
HAMAP	MF_00181. Cytosol_peptidase_M17. [Tree]
InterPro	IPR011356. Peptidase_M17. IPR000819. Peptidase_M17_C. IPR023042. Peptidase_M17_cytosol_amino. IPR008283. Peptidase_M17_N. [Graphical view]
KO	K01255.
PANTHER	PTHR11963:SF3. Peptidase_M17. 1 hit.
Pfam	PF00883. Peptidase_M17. 1 hit. PF02789. Peptidase_M17_N. 1 hit. [Graphical view]
PRINTS	PR00481. LAMNOPPTDASE.
PROSITE	PS00631. CYTOSOL_AP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPA_AERPE

Accession

Primary (citable) accession number: Q9Y935

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	July 10, 2007
Last modified:	November 16, 2011
This is version 69 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program