Reviewed, UniProtKB/Swiss-Prot Q9Y933 (GUAA_AERPE)

Names and origin

Protein names

Recommended name:
    GMP synthase [glutamine-hydrolyzing]
    EC=6.3.5.2
Alternative name(s):
    Glutamine amidotransferase
    GMP synthetase

Gene names

Name:	guaA
Ordered Locus Names:	APE_2452

Organism

Aeropyrum pernix [Complete proteome] [HAMAP]

Taxonomic identifier

56636 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Desulfurococcales › Desulfurococcaceae › Aeropyrum

Protein attributes

Sequence length	512 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the synthesis of GMP from XMP By similarity. HAMAP MF_00344
Catalytic activity	ATP + xanthosine 5'-phosphate + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate. HAMAP MF_00344
Pathway	Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1. HAMAP MF_00344
Sequence similarities	Contains 1 glutamine amidotransferase type-1 domain. Contains 1 GMP-binding domain.

Ontologies

Keywords
Biological process	GMP biosynthesis Purine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	GMP biosynthetic process Inferred from electronic annotation. Source: HAMAP NAD biosynthetic process Inferred from electronic annotation. Source: InterPro glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP GMP synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 512	512	GMP synthase [glutamine-hydrolyzing] HAMAP MF_00344		PRO_0000140215
Regions
	Domain	9 – 199	191	Glutamine amidotransferase type-1
	Domain	230 – 388	159	GMP-binding
	Nucleotide binding	226 – 232	7	ATP By similarity
Sites
	Active site	87	1	Nucleophile By similarity
	Active site	173	1	By similarity
	Active site	175	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9Y933-1 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: 7F5DE439B4C87368	FASTA	512	55,735
	10 20 30 40 50 60 MAKTLDSPGV LVVDFGGQYA HLIARRIREL GVYSEIVPAT SLDALGEALP RAAGVVLSGG 70 80 90 100 110 120 PGSVWGSRHD EAAAMVLQLG KPVLGICYGH QLLAKVLGGE VGRSPLPEFG PTEVEVLDYG 130 140 150 160 170 180 ILLNGLPSRF KVWMSHYDAV LRPPGEAKVL ARTPGSPVAA MELWGRVFGV QWHPEVRHTQ 190 200 210 220 230 240 YGREVLDNWL SLVGAPRTWR PGDMVSELVE SVKKEVGDAL AVAAVSGGVD STVAALIAKK 250 260 270 280 290 300 AIGSRLYPVF IDHGLHPEGE VERVVKLLSR LGLEPVMVDA GEEFLAALEG VGDPEEKRRV 310 320 330 340 350 360 VGRVYAEVLE RAARDIGAEY LVQGTIYPDV IESGARPGAD TIKTHHNVGG LPKDMRLKLV 370 380 390 400 410 420 EPLRYFYKDE VRLLAEKLGV PRELIWKQPV PGPGLAVRVE GPITREKLRI VRRADAIVRE 430 440 450 460 470 480 EVEAAGLGGK LWQYFAVLTA SMATGVRGDS RSYGYVVAVR AVESVDAMTA KPAELPWWLL 490 500 510 ERIARRITSE IPEVVRVVYD ITSKPPSTIE WE

References

[1]

"Complete genome sequence of an aerobic hyper-thermophilic crenarchaeon, Aeropyrum pernix K1." Kawarabayasi Y., Hino Y., Horikawa H., Yamazaki S., Haikawa Y., Jin-no K., Takahashi M., Sekine M., Baba S., Ankai A., Kosugi H., Hosoyama A., Fukui S., Nagai Y., Nishijima K., Nakazawa H., Takamiya M., Masuda S. , Funahashi T., Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Kubota K., Nakamura Y., Nomura N., Sako Y., Kikuchi H. DNA Res. 6:83-101(1999) [PubMed: 10382966] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000002 Genomic DNA. Translation: BAA81467.1.
PIR	C72476.
RefSeq	NP_148620.1.
3D structure databases
SMR	Q9Y933. Positions 8-200, 10-512.
ModBase	Search...
Genome annotation databases
GeneID	1445429.
GenomeReviews	Gene locus APE_2452 in contig BA000002_GR.
KEGG	ape:APE_2452.
NMPDR	fig|272557.1.peg.1728.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG391775.
OMA	MKLVEPL.
Enzyme and pathway databases
BioCyc	APER272557:APE2452-MONOMER.
BRENDA	6.3.5.2. 256344.
Family and domain databases
HAMAP	MF_00344. GMP_synthase. [Tree]
InterPro	IPR006220. Anth_synthII. IPR001317. CarbamoylP_synth_GATase_dom. IPR011702. GATASE. IPR017926. GATASE_1. IPR000991. GATase_class1_C. IPR001674. GMP_synth_C. IPR004739. GMP_synth_N. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF00117. GATase. 1 hit. PF00958. GMP_synt_C. 1 hit. [Graphical view]
PRINTS	PR00097. ANTSNTHASEII. PR00099. CPSGATASE. PR00096. GATASE.
TIGRFAMs	TIGR00884. guaA_Cterm. 1 hit. TIGR00888. guaA_Nterm. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUAA_AERPE

Accession

Primary (citable) accession number: Q9Y933

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	November 1, 1999
Last modified:	February 9, 2010
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)