Reviewed, UniProtKB/Swiss-Prot Q9Y8K5 (MTRF_METBF)

Names and origin

Protein names

Recommended name:
    Tetrahydromethanopterin S-methyltransferase subunit F
    EC=2.1.1.86
Alternative name(s):
    N5-methyltetrahydromethanopterin--coenzyme M methyltransferase subunit F

Gene names

Name:	mtrF
Ordered Locus Names:	Mbar_A1257

Organism

Methanosarcina barkeri (strain Fusaro / DSM 804) [Complete proteome] [HAMAP]

Taxonomic identifier

269797 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length	73 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Part of a complex that catalyzes the formation of methyl-coenzyme M and tetrahydromethanopterin from coenzyme M and methyl-tetrahydromethanopterin. This is an energy-conserving, sodium-ion translocating step By similarity.
Catalytic activity	5-methyl-5,6,7,8-tetrahydromethanopterin + 2-mercaptoethanesulfonate = 5,6,7,8-tetrahydromethanopterin + 2-(methylthio)ethanesulfonate. HAMAP MF_01099
Pathway	One-carbon metabolism; methanogenesis from carbone dioxide; methyl-CoM from 5,10-methylene-H(4)MPT: step 2/2. HAMAP MF_01099
Subunit structure	The complex is composed of 8 subunits; mtrA, mtrB, mtrC, mtrD, mtrE, mtrF, mtrG and mtrH By similarity.
Subcellular location	Cell membrane; Single-pass membrane protein Potential.
Sequence similarities	Belongs to the mtrF family.

Ontologies

Keywords
Biological process	Methanogenesis One-carbon metabolism
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methanogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	tetrahydromethanopterin S-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 73	73	Tetrahydromethanopterin S-methyltransferase subunit F HAMAP MF_01099		PRO_0000147547
Regions
	Transmembrane	52 – 72	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9Y8K5-1 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: C74C43B6B0310038	FASTA	73	7,978
	10 20 30 40 50 60 MKVAEEYDKG VPMMLAPQMG AIDATVESIR YRAQLIARNQ KLDSGVAATG MIGFAAGFLF 70 SLLMVIVLPL LFW

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The energy conserving methyltetrahydromethanopterin:coenzyme M methyltransferase complex from methanogenic archaea: function of the subunit MtrH." Hippler B., Thauer R.K. FEBS Lett. 449:165-168(1999) [PubMed: 10338124] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The Methanosarcina barkeri genome: comparative analysis with Methanosarcina acetivorans and Methanosarcina mazei reveals extensive rearrangement within methanosarcinal genomes." Maeder D.L., Anderson I., Brettin T.S., Bruce D.C., Gilna P., Han C.S., Lapidus A., Metcalf W.W., Saunders E., Tapia R., Sowers K.R. J. Bacteriol. 188:7922-7931(2006) [PubMed: 16980466] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ132817 Genomic DNA. Translation: CAB41643.1. CP000099 Genomic DNA. Translation: AAZ70220.1.
RefSeq	YP_304800.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3627857.
GenomeReviews	Gene locus Mbar_A1257 in contig CP000099_GR.
KEGG	mba:Mbar_A1257.
NMPDR	fig|269797.3.peg.1263.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9Y8K5.
OMA	Q9Y8K5. ILARTNK.
Enzyme and pathway databases
BioCyc	MBAR269797:MBAR_A1257-MON.
Family and domain databases
HAMAP	MF_01099. [Tree]
InterPro	IPR011307. MeTrfase_F. IPR013347. MeTrfase_F_su. [Graphical view]
Pfam	PF09472. MtrF. 1 hit. [Graphical view]
PIRSF	PIRSF006523. MtrF. 1 hit.
TIGRFAMs	TIGR02507. MtrF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MTRF_METBF

Accession

Primary (citable) accession number: Q9Y8K5
Secondary accession number(s): Q46D22

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	November 1, 1999
Last modified:	June 16, 2009
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)