Q9Y8K5 (MTRF_METBF) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tetrahydromethanopterin S-methyltransferase subunit F
EC=2.1.1.86

Alternative name(s):
N5-methyltetrahydromethanopterin--coenzyme M methyltransferase subunit F

Gene names

Name:	mtrF
Ordered Locus Names:	Mbar_A1257

Organism

Methanosarcina barkeri (strain Fusaro / DSM 804) [Complete proteome] [HAMAP]

Taxonomic identifier

269797 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length	73 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Part of a complex that catalyzes the formation of methyl-coenzyme M and tetrahydromethanopterin from coenzyme M and methyl-tetrahydromethanopterin. This is an energy-conserving, sodium-ion translocating step By similarity. HAMAP MF_01099
Catalytic activity	5-methyl-5,6,7,8-tetrahydromethanopterin + 2-mercaptoethanesulfonate = 5,6,7,8-tetrahydromethanopterin + 2-(methylthio)ethanesulfonate. HAMAP MF_01099
Pathway	One-carbon metabolism; methanogenesis from CO(2); methyl-coenzyme M from 5,10-methylene-5,6,7,8-tetrahydromethanopterin: step 2/2. HAMAP MF_01099
Subunit structure	The complex is composed of 8 subunits; mtrA, mtrB, mtrC, mtrD, mtrE, mtrF, mtrG and mtrH By similarity.
Subcellular location	Cell membrane; Single-pass membrane protein Potential HAMAP MF_01099.
Sequence similarities	Belongs to the mtrF family.

Ontologies

Keywords
Biological process	Methanogenesis One-carbon metabolism
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methanogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	tetrahydromethanopterin S-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 73	73	Tetrahydromethanopterin S-methyltransferase subunit F HAMAP MF_01099		PRO_0000147547
Regions
	Transmembrane	52 – 72	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9Y8K5 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: C74C43B6B0310038	FASTA	73	7,978
	10 20 30 40 50 60 MKVAEEYDKG VPMMLAPQMG AIDATVESIR YRAQLIARNQ KLDSGVAATG MIGFAAGFLF 70 SLLMVIVLPL LFW

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The energy conserving methyltetrahydromethanopterin:coenzyme M methyltransferase complex from methanogenic archaea: function of the subunit MtrH." Hippler B., Thauer R.K. FEBS Lett. 449:165-168(1999) [PubMed: 10338124] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The Methanosarcina barkeri genome: comparative analysis with Methanosarcina acetivorans and Methanosarcina mazei reveals extensive rearrangement within methanosarcinal genomes." Maeder D.L., Anderson I., Brettin T.S., Bruce D.C., Gilna P., Han C.S., Lapidus A., Metcalf W.W., Saunders E., Tapia R., Sowers K.R. J. Bacteriol. 188:7922-7931(2006) [PubMed: 16980466] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Fusaro / DSM 804.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ132817 Genomic DNA. Translation: CAB41643.1. CP000099 Genomic DNA. Translation: AAZ70220.1.
RefSeq	YP_304800.1. NC_007355.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q9Y8K5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3627857.
GenomeReviews	Gene locus Mbar_A1257 in contig CP000099_GR.
KEGG	mba:Mbar_A1257.
NMPDR	fig|269797.3.peg.1263.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG12738.
HOGENOM	HBG542040.
OMA	KLESGIM.
ProtClustDB	PRK13275.
Enzyme and pathway databases
BioCyc	MBAR269797:MBAR_A1257-MONOMER.
Family and domain databases
HAMAP	MF_01099. MtrF. [Tree]
InterPro	IPR011307. MeTrfase_F. IPR013347. MeTrfase_F_su. [Graphical view]
KO	K00582.
Pfam	PF09472. MtrF. 1 hit. [Graphical view]
PIRSF	PIRSF006523. MtrF. 1 hit.
TIGRFAMs	TIGR02507. MtrF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MTRF_METBF

Accession

Primary (citable) accession number: Q9Y8K5
Secondary accession number(s): Q46D22

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	November 1, 1999
Last modified:	November 16, 2011
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program