Reviewed, UniProtKB/Swiss-Prot Q9Y8K0 (MTRE_METBF)

Names and origin

Protein names

Recommended name:
    Tetrahydromethanopterin S-methyltransferase subunit E
    EC=2.1.1.86
Alternative name(s):
    N5-methyltetrahydromethanopterin--coenzyme M methyltransferase subunit E

Gene names

Name:	mtrE
Ordered Locus Names:	Mbar_A1262

Organism

Methanosarcina barkeri (strain Fusaro / DSM 804) [Complete proteome] [HAMAP]

Taxonomic identifier

269797 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length	302 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Part of a complex that catalyzes the formation of methyl-coenzyme M and tetrahydromethanopterin from coenzyme M and methyl-tetrahydromethanopterin. This is an energy-conserving, sodium-ion translocating step By similarity.
Catalytic activity	5-methyl-5,6,7,8-tetrahydromethanopterin + 2-mercaptoethanesulfonate = 5,6,7,8-tetrahydromethanopterin + 2-(methylthio)ethanesulfonate. HAMAP MF_01098
Pathway	One-carbon metabolism; methanogenesis from carbone dioxide; methyl-CoM from 5,10-methylene-H(4)MPT: step 2/2. HAMAP MF_01098
Subunit structure	The complex is composed of 8 subunits; mtrA, mtrB, mtrC, mtrD, mtrE, mtrF, mtrG and mtrH By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein Potential.
Sequence similarities	Belongs to the mtrE family.

Ontologies

Keywords
Biological process	Methanogenesis One-carbon metabolism
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methanogenesis Inferred from electronic annotation. Source: UniProtKB-KW sodium ion transport Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell vesicle membrane Inferred from electronic annotation. Source: InterPro
Molecular function	tetrahydromethanopterin S-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 302	302	Tetrahydromethanopterin S-methyltransferase subunit E HAMAP MF_01098		PRO_0000147537
Regions
	Transmembrane	3 – 23	21	Potential
	Transmembrane	86 – 106	21	Potential
	Transmembrane	132 – 152	21	Potential
	Transmembrane	155 – 175	21	Potential
	Transmembrane	233 – 253	21	Potential
	Transmembrane	259 – 279	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9Y8K0-1 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: 78BE4AE4E0A9A182	FASTA	302	32,507
	10 20 30 40 50 60 MEPLISMGVL ALIGVAATIA GASEDLESDI GSQSNPNSQV QLAPQMMFPH RIFNKAISGE 70 80 90 100 110 120 PPSNALMCSI GAAVATVLIS EFTMSPLFAL VFGSLIAACV HATFAVTSTM GRCASQSRFK 130 140 150 160 170 180 QPIYLDMIRS HITPIMGYAF ITTFCILVVS YLMTVVLGHP FPLTMLAFIW GITIGAIGSS 190 200 210 220 230 240 TGDVHYGAER EFQQFEFGSG LNASNSGNIV RYAESGLRDG FDNSWFCAKF GGPVTGLAFG 250 260 270 280 290 300 MTVFLGSWIT TIFDPAKGLG WLSVIAGIVI VFILIIWNWK MEVYARKAYG PYKEDKTEEA SA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The energy conserving methyltetrahydromethanopterin:coenzyme M methyltransferase complex from methanogenic archaea: function of the subunit MtrH." Hippler B., Thauer R.K. FEBS Lett. 449:165-168(1999) [PubMed: 10338124] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The Methanosarcina barkeri genome: comparative analysis with Methanosarcina acetivorans and Methanosarcina mazei reveals extensive rearrangement within methanosarcinal genomes." Maeder D.L., Anderson I., Brettin T.S., Bruce D.C., Gilna P., Han C.S., Lapidus A., Metcalf W.W., Saunders E., Tapia R., Sowers K.R. J. Bacteriol. 188:7922-7931(2006) [PubMed: 16980466] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ132817 Genomic DNA. Translation: CAB41638.1. CP000099 Genomic DNA. Translation: AAZ70225.1.
RefSeq	YP_304805.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3627862.
GenomeReviews	Gene locus Mbar_A1262 in contig CP000099_GR.
KEGG	mba:Mbar_A1262.
NMPDR	fig|269797.3.peg.1268.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9Y8K0.
OMA	Q9Y8K0. SNPNSQV.
Enzyme and pathway databases
BioCyc	MBAR269797:MBAR_A1262-MON.
Family and domain databases
HAMAP	MF_01098. [Tree]
InterPro	IPR005780. MeTrfase_E. [Graphical view]
Pfam	PF04206. MtrE. 1 hit. [Graphical view]
PIRSF	PIRSF016509. MtrE. 1 hit.
TIGRFAMs	TIGR01113. mtrE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MTRE_METBF

Accession

Primary (citable) accession number: Q9Y8K0
Secondary accession number(s): Q46D17

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	November 1, 1999
Last modified:	June 16, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)