Glutamate dehydrogenase - Pyrobaculum islandicum

Preferred order of sections

Names and origin

Protein names	Recommended name: Glutamate dehydrogenase PIRNR PIRNR000185
Organism	Pyrobaculum islandicum EMBL BAA77715.1
Taxonomic identifier	2277 [NCBI]
Taxonomic lineage	Archaea › Crenarchaeota › Thermoprotei › Thermoproteales › Thermoproteaceae › Pyrobaculum

Protein attributes

Sequence length	421 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Sequence similarities

Belongs to the Glu/Leu/Phe/Val dehydrogenases family. RuleBase RU004417 PIRNR PIRNR000185

Ontologies

Keywords
Ligand	NAD PDB 1V9L Nucleotide-binding PDB 1V9L
Molecular function	Oxidoreductase PIRNR PIRNR000185
Technical term	3D-structure PDB 1V9L
Gene Ontology (GO)
Biological process	cellular amino acid metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW oxidoreductase activity, acting on the CH-NH2 group of donors, NAD or NADP as acceptor Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Regions

Nucleotide binding

220 – 221

2

NAD PDB 1V9L

Sites

Active site

105

1

By similarity PIRSR PIRSR000185-1

Binding site

189

1

NAD PDB 1V9L

Binding site

216

1

NAD PDB 1V9L

Binding site

241

1

NAD PDB 1V9L

Binding site

299

1

NAD PDB 1V9L

Binding site

322

1

NAD PDB 1V9L

Binding site

347

1

NAD PDB 1V9L

Sequences

Sequence

Length

Mass (Da)

Tools

Q9Y8I4 [UniParc].

Last modified November 1, 1999. Version 1.
Checksum: 06B2C23176FE7548
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FASTA

421

46,906

        10         20         30         40         50         60 
MERTGFLEYV LNYVKKGVEL GGFPEDFYKI LSRPRRVLIV NIPVRLDGGG FEVFEGYRVQ 

        70         80         90        100        110        120 
HCDVLGPYKG GVRFHPEVTL ADDVALAILM TLKNSLAGLP YGGAKGAVRV DPKKLSQREL 

       130        140        150        160        170        180 
EELSRGYARA IAPLIGDVVD IPAPDVGTNA QIMAWMVDEY SKIKGYNVPG VFTSKPPELW 

       190        200        210        220        230        240 
GNPVREYATG FGVAVATREM AKKLWGGIEG KTVAIQGMGN VGRWTAYWLE KMGAKVIAVS 

       250        260        270        280        290        300 
DINGVAYRKE GLNVELIQKN KGLTGPALVE LFTTKDNAEF VKNPDAIFKL DVDIFVPAAI 

       310        320        330        340        350        360 
ENVIRGDNAG LVKARLVVEG ANGPTTPEAE RILYERGVVV VPDILANAGG VIMSYLEWVE 

       370        380        390        400        410        420 
NLQWYIWDEE ETRKRLENIM VNNVERVYKR WQREKGWTMR DAAIVTALER IYNAMKIRGW 


I

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References

[1]	"The NAD-dependent glutamate dehydrogenase from the hyperthermophilic archaeon Pyrobaculum islandicum: cloning, sequencing, and expression of the enzyme gene(1)." Kujo C., Sakuraba H., Nunoura N., Ohshima T. Biochim. Biophys. Acta 1434:365-371(1999) [PubMed: 10525154] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[2]	"The first crystal structure of hyperthermostable NAD-dependent glutamate dehydrogenase from Pyrobaculum islandicum." Bhuiya M.W., Sakuraba H., Ohshima T., Imagawa T., Katunuma N., Tsuge H. J. Mol. Biol. 345:325-337(2005) [PubMed: 15571725] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.80 ANGSTROMS) IN COMPLEX WITH NAD.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AB027194 Genomic DNA. Translation: BAA77715.1.

PIR

T43790.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1V9L	X-ray	2.80	A/B/C/D/E/F	1-421	[»]

ProteinModelPortal

Q9Y8I4.

SMR

Q9Y8I4. Positions 4-421.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR006095. Glu/Leu/Phe/Val_DH.
IPR006096. Glu/Leu/Phe/Val_DH_C.
IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom.
IPR014362. Glu_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view]

Gene3D

G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.

Pfam

PF00208. ELFV_dehydrog. 1 hit.
PF02812. ELFV_dehydrog_N. 1 hit.
[Graphical view]

PIRSF

PIRSF000185. Glu_DH. 1 hit.

PRINTS

PR00082. GLFDHDRGNASE.

SMART

SM00839. ELFV_dehydrog. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

Q9Y8I4_PYRIS

Accession

Primary (citable) accession number: Q9Y8I4

Entry history

Integrated into UniProtKB/TrEMBL:	November 1, 1999
Last sequence update:	November 1, 1999
Last modified:	November 16, 2011
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)