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Q9Y8G7

- C505_FUSOX

UniProt

Q9Y8G7 - C505_FUSOX

Protein

Bifunctional P-450:NADPH-P450 reductase

Gene

CYP505

Organism
Fusarium oxysporum (Fusarium vascular wilt)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 87 (01 Oct 2014)
      Sequence version 1 (01 Nov 1999)
      Previous versions | rss
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    Functioni

    Functions as a fatty acid monooxygenase. Shows highest activity toward fatty acids with a chain length of 12-14 carbons. The reductase domain is required for electron transfer from NADP to cytochrome P450.1 Publication

    Catalytic activityi

    RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.
    NADPH + n oxidized hemoprotein = NADP+ + n reduced hemoprotein.

    Cofactori

    Binds 1 FAD.By similarity
    Binds 1 FMN.By similarity
    Heme group.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi407 – 4071Iron (heme axial ligand)By similarity

    GO - Molecular functioni

    1. aromatase activity Source: UniProtKB-EC
    2. FMN binding Source: InterPro
    3. heme binding Source: InterPro
    4. iron ion binding Source: InterPro
    5. NADPH-hemoprotein reductase activity Source: UniProtKB-EC

    Keywords - Molecular functioni

    Monooxygenase, Oxidoreductase

    Keywords - Biological processi

    Electron transport, Transport

    Keywords - Ligandi

    FAD, Flavoprotein, FMN, Heme, Iron, Metal-binding, NADP

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Bifunctional P-450:NADPH-P450 reductase
    Alternative name(s):
    Cytochrome P450foxy
    Fatty acid omega-hydroxylase
    Including the following 2 domains:
    Cytochrome P450 505 (EC:1.14.14.1)
    NADPH--cytochrome P450 reductase (EC:1.6.2.4)
    Gene namesi
    Name:CYP505
    OrganismiFusarium oxysporum (Fusarium vascular wilt)
    Taxonomic identifieri5507 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesHypocreomycetidaeHypocrealesNectriaceaeFusariumFusarium oxysporum species complex

    Subcellular locationi

    Membrane 2 Publications; Peripheral membrane protein 2 Publications

    GO - Cellular componenti

    1. membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10661066Bifunctional P-450:NADPH-P450 reductasePRO_0000052210Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliQ9Y8G7.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini500 – 641142Flavodoxin-likePROSITE-ProRule annotationAdd
    BLAST
    Domaini676 – 904229FAD-binding FR-typePROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 480480Cytochrome P450Add
    BLAST
    Regioni481 – 1066586NADPH-P-450 reductaseAdd
    BLAST

    Sequence similaritiesi

    In the N-terminal section; belongs to the cytochrome P450 family.Curated
    Contains 1 FAD-binding FR-type domain.PROSITE-ProRule annotation
    Contains 1 flavodoxin-like domain.PROSITE-ProRule annotation

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    1.20.990.10. 1 hit.
    3.40.50.360. 1 hit.
    InterProiIPR023206. Bifunctional_P450_P450_red.
    IPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    IPR003097. FAD-binding_1.
    IPR017927. Fd_Rdtase_FAD-bd.
    IPR008254. Flavodoxin/NO_synth.
    IPR029039. Flavoprotein-like.
    IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
    IPR001433. OxRdtase_FAD/NAD-bd.
    IPR017938. Riboflavin_synthase-like_b-brl.
    [Graphical view]
    PfamiPF00667. FAD_binding_1. 1 hit.
    PF00258. Flavodoxin_1. 1 hit.
    PF00175. NAD_binding_1. 1 hit.
    PF00067. p450. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000209. Bifunctional_P450_P450R. 1 hit.
    PRINTSiPR00463. EP450I.
    PR00385. P450.
    SUPFAMiSSF48264. SSF48264. 1 hit.
    SSF52218. SSF52218. 1 hit.
    SSF63380. SSF63380. 1 hit.
    PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
    PS51384. FAD_FR. 1 hit.
    PS50902. FLAVODOXIN_LIKE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9Y8G7-1 [UniParc]FASTAAdd to Basket

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    MAESVPIPEP PGYPLIGNLG EFTSNPLSDL NRLADTYGPI FRLRLGAKAP     50
    IFVSSNSLIN EVCDEKRFKK TLKSVLSQVR EGVHDGLFTA FEDEPNWGKA 100
    HRILVPAFGP LSIRGMFPEM HDIATQLCMK FARHGPRTPI DTSDNFTRLA 150
    LDTLALCAMD FRFYSYYKEE LHPFIEAMGD FLTESGNRNR RPPFAPNFLY 200
    RAANEKFYGD IALMKSVADE VVAARKASPS DRKDLLAAML NGVDPQTGEK 250
    LSDENITNQL ITFLIAGHET TSGTLSFAMY QLLKNPEAYS KVQKEVDEVV 300
    GRGPVLVEHL TKLPYISAVL RETLRLNSPI TAFGLEAIDD TFLGGKYLVK 350
    KGEIVTALLS RGHVDPVVYG NDADKFIPER MLDDEFARLN KEYPNCWKPF 400
    GNGKRACIGR PFAWQESLLA MVVLFQNFNF TMTDPNYALE IKQTLTIKPD 450
    HFYINATLRH GMTPTELEHV LAGNGATSSS THNIKAAANL DAKAGSGKPM 500
    AIFYGSNSGT CEALANRLAS DAPSHGFSAT TVGPLDQAKQ NLPEDRPVVI 550
    VTASYEGQPP SNAAHFIKWM EDLDGNDMEK VSYAVFACGH HDWVETFHRI 600
    PKLVDSTLEK RGGTRLVPMG SADAATSDMF SDFEAWEDIV LWPGLKEKYK 650
    ISDEESGGQK GLLVEVSTPR KTSLRQDVEE ALVVAEKTLT KSGPAKKHIE 700
    IQLPSAMTYK AGDYLAILPL NPKSTVARVF RRFSLAWDSF LKIQSEGPTT 750
    LPTNVAISAF DVFSAYVELS QPATKRNILA LAEATEDKDT IQELERLAGD 800
    AYQAEISPKR VSVLDLLEKF PAVALPISSY LAMLPPMRVR QYSISSSPFA 850
    DPSKLTLTYS LLDAPSLSGQ GRHVGVATNF LSHLTAGDKL HVSVRASSEA 900
    FHLPSDAEKT PIICVAAGTG LAPLRGFIQE RAAMLAAGRT LAPALLFFGC 950
    RNPEIDDLYA EEFERWEKMG AVDVRRAYSR ATDKSEGCKY VQDRVYHDRA 1000
    DVFKVWDQGA KVFICGSREI GKAVEDVCVR LAIEKAQQNG RDVTEEMARA 1050
    WFERSRNERF ATDVFD 1066
    Length:1,066
    Mass (Da):117,926
    Last modified:November 1, 1999 - v1
    Checksum:i6B8123698C223DBA
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB030037 Genomic DNA. Translation: BAA82526.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB030037 Genomic DNA. Translation: BAA82526.1 .

    3D structure databases

    ProteinModelPortali Q9Y8G7.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 1.10.630.10. 1 hit.
    1.20.990.10. 1 hit.
    3.40.50.360. 1 hit.
    InterProi IPR023206. Bifunctional_P450_P450_red.
    IPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    IPR003097. FAD-binding_1.
    IPR017927. Fd_Rdtase_FAD-bd.
    IPR008254. Flavodoxin/NO_synth.
    IPR029039. Flavoprotein-like.
    IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
    IPR001433. OxRdtase_FAD/NAD-bd.
    IPR017938. Riboflavin_synthase-like_b-brl.
    [Graphical view ]
    Pfami PF00667. FAD_binding_1. 1 hit.
    PF00258. Flavodoxin_1. 1 hit.
    PF00175. NAD_binding_1. 1 hit.
    PF00067. p450. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000209. Bifunctional_P450_P450R. 1 hit.
    PRINTSi PR00463. EP450I.
    PR00385. P450.
    SUPFAMi SSF48264. SSF48264. 1 hit.
    SSF52218. SSF52218. 1 hit.
    SSF63380. SSF63380. 1 hit.
    PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
    PS51384. FAD_FR. 1 hit.
    PS50902. FLAVODOXIN_LIKE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Fusarium oxysporum fatty-acid subterminal hydroxylase (CYP505) is a membrane-bound eukaryotic counterpart of Bacillus megaterium cytochrome P450BM3."
      Kitazume T., Takaya N., Nakayama N., Shoun H.
      J. Biol. Chem. 275:39734-39740(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 82-99; 353-359; 362-380; 518-538; 616-626 AND 1005-1009, SUBCELLULAR LOCATION.
      Strain: MT-811.
    2. "Cytochrome P450foxy, a catalytically self-sufficient fatty acid hydroxylase of the fungus Fusarium oxysporum."
      Nakayama N., Takemae A., Shoun H.
      J. Biochem. 119:435-440(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
      Strain: MT-811.

    Entry informationi

    Entry nameiC505_FUSOX
    AccessioniPrimary (citable) accession number: Q9Y8G7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 30, 2002
    Last sequence update: November 1, 1999
    Last modified: October 1, 2014
    This is version 87 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing, Multifunctional enzyme

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3