Reviewed, UniProtKB/Swiss-Prot Q9Y8G7 (C505_FUSOX)

Names and origin

Protein names

Recommended name:
    Bifunctional P-450:NADPH-P450 reductase
Alternative name(s):
    Fatty acid omega-hydroxylase
    P450foxy
Including the following 2 domains:
    1- Recommended name:
            Cytochrome P450 505
              EC=1.14.14.1
    2- Recommended name:
            NADPH--cytochrome P450 reductase
              EC=1.6.2.4

Gene names

Name:

CYP505

Organism

Fusarium oxysporum

Taxonomic identifier

5507 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Hypocreomycetidae › Hypocreales › mitosporic Hypocreales › Fusarium › Fusarium oxysporum species complex

Protein attributes

Sequence length	1066 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Functions as a fatty acid monooxygenase. Shows highest activity toward fatty acids with a chain length of 12-14 carbons. The reductase domain is required for electron transfer from NADP to cytochrome P450.
Catalytic activity	RH + reduced flavoprotein + O(2) = ROH + oxidized flavoprotein + H(2)O. NADPH + n oxidized hemoprotein = NADP(+) + n reduced hemoprotein.
Cofactor	Binds 1 FAD By similarity. Binds 1 FMN By similarity. Heme group By similarity.
Subcellular location	Membrane; Peripheral membrane protein.
Sequence similarities	In the N-terminal section; belongs to the cytochrome P450 family. Contains 1 FAD-binding FR-type domain. Contains 1 flavodoxin-like domain.

Ontologies

Keywords
Biological process	Electron transport Transport
Cellular component	Membrane
Ligand	FAD FMN Flavoprotein Heme Iron Metal-binding NADP
Molecular function	Monooxygenase Oxidoreductase
Technical term	Direct protein sequencing Multifunctional enzyme
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NADPH-hemoprotein reductase activity Inferred from electronic annotation. Source: EC unspecific monooxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1066	1066	Bifunctional P-450:NADPH-P450 reductase		PRO_0000052210
Regions
	Domain	500 – 641	142	Flavodoxin-like
	Domain	676 – 904	229	FAD-binding FR-type
	Region	1 – 480	480	Cytochrome P450
	Region	481 – 1066	586	NADPH-P-450 reductase
Sites
	Metal binding	407	1	Iron (heme axial ligand) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9Y8G7-1 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: 6B8123698C223DBA	FASTA	1,066	117,926
	10 20 30 40 50 60 MAESVPIPEP PGYPLIGNLG EFTSNPLSDL NRLADTYGPI FRLRLGAKAP IFVSSNSLIN 70 80 90 100 110 120 EVCDEKRFKK TLKSVLSQVR EGVHDGLFTA FEDEPNWGKA HRILVPAFGP LSIRGMFPEM 130 140 150 160 170 180 HDIATQLCMK FARHGPRTPI DTSDNFTRLA LDTLALCAMD FRFYSYYKEE LHPFIEAMGD 190 200 210 220 230 240 FLTESGNRNR RPPFAPNFLY RAANEKFYGD IALMKSVADE VVAARKASPS DRKDLLAAML 250 260 270 280 290 300 NGVDPQTGEK LSDENITNQL ITFLIAGHET TSGTLSFAMY QLLKNPEAYS KVQKEVDEVV 310 320 330 340 350 360 GRGPVLVEHL TKLPYISAVL RETLRLNSPI TAFGLEAIDD TFLGGKYLVK KGEIVTALLS 370 380 390 400 410 420 RGHVDPVVYG NDADKFIPER MLDDEFARLN KEYPNCWKPF GNGKRACIGR PFAWQESLLA 430 440 450 460 470 480 MVVLFQNFNF TMTDPNYALE IKQTLTIKPD HFYINATLRH GMTPTELEHV LAGNGATSSS 490 500 510 520 530 540 THNIKAAANL DAKAGSGKPM AIFYGSNSGT CEALANRLAS DAPSHGFSAT TVGPLDQAKQ 550 560 570 580 590 600 NLPEDRPVVI VTASYEGQPP SNAAHFIKWM EDLDGNDMEK VSYAVFACGH HDWVETFHRI 610 620 630 640 650 660 PKLVDSTLEK RGGTRLVPMG SADAATSDMF SDFEAWEDIV LWPGLKEKYK ISDEESGGQK 670 680 690 700 710 720 GLLVEVSTPR KTSLRQDVEE ALVVAEKTLT KSGPAKKHIE IQLPSAMTYK AGDYLAILPL 730 740 750 760 770 780 NPKSTVARVF RRFSLAWDSF LKIQSEGPTT LPTNVAISAF DVFSAYVELS QPATKRNILA 790 800 810 820 830 840 LAEATEDKDT IQELERLAGD AYQAEISPKR VSVLDLLEKF PAVALPISSY LAMLPPMRVR 850 860 870 880 890 900 QYSISSSPFA DPSKLTLTYS LLDAPSLSGQ GRHVGVATNF LSHLTAGDKL HVSVRASSEA 910 920 930 940 950 960 FHLPSDAEKT PIICVAAGTG LAPLRGFIQE RAAMLAAGRT LAPALLFFGC RNPEIDDLYA 970 980 990 1000 1010 1020 EEFERWEKMG AVDVRRAYSR ATDKSEGCKY VQDRVYHDRA DVFKVWDQGA KVFICGSREI 1030 1040 1050 1060 GKAVEDVCVR LAIEKAQQNG RDVTEEMARA WFERSRNERF ATDVFD

References

[1]

"Fusarium oxysporum fatty-acid subterminal hydroxylase (CYP505) is a membrane-bound eukaryotic counterpart of Bacillus megaterium cytochrome P450BM3." Kitazume T., Takaya N., Nakayama N., Shoun H. J. Biol. Chem. 275:39734-39740(2000) [PubMed: 10995755] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 82-99; 353-359; 362-380; 518-538; 616-626 AND 1005-1009, SUBCELLULAR LOCATION. Strain: MT-811.

[2]

"Cytochrome P450foxy, a catalytically self-sufficient fatty acid hydroxylase of the fungus Fusarium oxysporum." Nakayama N., Takemae A., Shoun H. J. Biochem. 119:435-440(1996) [PubMed: 8830036] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION. Strain: MT-811.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB030037 Genomic DNA. Translation: BAA82526.1.
3D structure databases
HSSP	HSSP built from PDB template 1JPZ based on UniProtKB P14779.
ModBase	Search...
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR002401. Cyt_P450_E_grp-I. IPR003097. FAD-binding_1. IPR001094. Flavdoxin_like. IPR008254. Flavodoxin/NO_synth. IPR001709. FPN_cyt_redctse. IPR015702. NADPH_Cyt_Red. IPR001433. OxRdtase_FAD/NAD_bd. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19384:SF17. NADPH_Cyt_Red. 1 hit.
Pfam	PF00667. FAD_binding_1. 1 hit. PF00258. Flavodoxin_1. 1 hit. PF00175. NAD_binding_1. 1 hit. PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00369. FLAVODOXIN. PR00371. FPNCR. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. PS51384. FAD_FR. 1 hit. PS50902. FLAVODOXIN_LIKE. 1 hit. [Graphical view]
BLOCKS	Search...
ProtoNet	Search...

Entry information

Entry name

C505_FUSOX

Accession

Primary (citable) accession number: Q9Y8G7

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2002
Last sequence update:	November 1, 1999
Last modified:	November 4, 2008
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)