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Q9Y8G7

- C505_FUSOX

UniProt

Q9Y8G7 - C505_FUSOX

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Protein

Bifunctional P-450:NADPH-P450 reductase

Gene
CYP505
Organism
Fusarium oxysporum (Fusarium vascular wilt)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Functions as a fatty acid monooxygenase. Shows highest activity toward fatty acids with a chain length of 12-14 carbons. The reductase domain is required for electron transfer from NADP to cytochrome P450.1 Publication

Catalytic activityi

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.
NADPH + n oxidized hemoprotein = NADP+ + n reduced hemoprotein.

Cofactori

Binds 1 FAD By similarity.
Binds 1 FMN By similarity.
Heme group By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi407 – 4071Iron (heme axial ligand) By similarity

GO - Molecular functioni

  1. aromatase activity Source: UniProtKB-EC
  2. FMN binding Source: InterPro
  3. heme binding Source: InterPro
  4. iron ion binding Source: InterPro
  5. NADPH-hemoprotein reductase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Electron transport, Transport

Keywords - Ligandi

FAD, Flavoprotein, FMN, Heme, Iron, Metal-binding, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional P-450:NADPH-P450 reductase
Alternative name(s):
Cytochrome P450foxy
Fatty acid omega-hydroxylase
Including the following 2 domains:
Cytochrome P450 505 (EC:1.14.14.1)
NADPH--cytochrome P450 reductase (EC:1.6.2.4)
Gene namesi
Name:CYP505
OrganismiFusarium oxysporum (Fusarium vascular wilt)
Taxonomic identifieri5507 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesHypocreomycetidaeHypocrealesNectriaceaeFusariumFusarium oxysporum species complex

Subcellular locationi

Membrane; Peripheral membrane protein 2 Publications

GO - Cellular componenti

  1. membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10661066Bifunctional P-450:NADPH-P450 reductasePRO_0000052210Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ9Y8G7.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini500 – 641142Flavodoxin-likeAdd
BLAST
Domaini676 – 904229FAD-binding FR-typeAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 480480Cytochrome P450Add
BLAST
Regioni481 – 1066586NADPH-P-450 reductaseAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the cytochrome P450 family.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
1.20.990.10. 1 hit.
3.40.50.360. 1 hit.
InterProiIPR023206. Bifunctional_P450_P450_red.
IPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR008254. Flavodoxin/NO_synth.
IPR029039. Flavoprotein-like.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
PF00067. p450. 1 hit.
[Graphical view]
PIRSFiPIRSF000209. Bifunctional_P450_P450R. 1 hit.
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
SSF52218. SSF52218. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
PS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9Y8G7-1 [UniParc]FASTAAdd to Basket

« Hide

MAESVPIPEP PGYPLIGNLG EFTSNPLSDL NRLADTYGPI FRLRLGAKAP     50
IFVSSNSLIN EVCDEKRFKK TLKSVLSQVR EGVHDGLFTA FEDEPNWGKA 100
HRILVPAFGP LSIRGMFPEM HDIATQLCMK FARHGPRTPI DTSDNFTRLA 150
LDTLALCAMD FRFYSYYKEE LHPFIEAMGD FLTESGNRNR RPPFAPNFLY 200
RAANEKFYGD IALMKSVADE VVAARKASPS DRKDLLAAML NGVDPQTGEK 250
LSDENITNQL ITFLIAGHET TSGTLSFAMY QLLKNPEAYS KVQKEVDEVV 300
GRGPVLVEHL TKLPYISAVL RETLRLNSPI TAFGLEAIDD TFLGGKYLVK 350
KGEIVTALLS RGHVDPVVYG NDADKFIPER MLDDEFARLN KEYPNCWKPF 400
GNGKRACIGR PFAWQESLLA MVVLFQNFNF TMTDPNYALE IKQTLTIKPD 450
HFYINATLRH GMTPTELEHV LAGNGATSSS THNIKAAANL DAKAGSGKPM 500
AIFYGSNSGT CEALANRLAS DAPSHGFSAT TVGPLDQAKQ NLPEDRPVVI 550
VTASYEGQPP SNAAHFIKWM EDLDGNDMEK VSYAVFACGH HDWVETFHRI 600
PKLVDSTLEK RGGTRLVPMG SADAATSDMF SDFEAWEDIV LWPGLKEKYK 650
ISDEESGGQK GLLVEVSTPR KTSLRQDVEE ALVVAEKTLT KSGPAKKHIE 700
IQLPSAMTYK AGDYLAILPL NPKSTVARVF RRFSLAWDSF LKIQSEGPTT 750
LPTNVAISAF DVFSAYVELS QPATKRNILA LAEATEDKDT IQELERLAGD 800
AYQAEISPKR VSVLDLLEKF PAVALPISSY LAMLPPMRVR QYSISSSPFA 850
DPSKLTLTYS LLDAPSLSGQ GRHVGVATNF LSHLTAGDKL HVSVRASSEA 900
FHLPSDAEKT PIICVAAGTG LAPLRGFIQE RAAMLAAGRT LAPALLFFGC 950
RNPEIDDLYA EEFERWEKMG AVDVRRAYSR ATDKSEGCKY VQDRVYHDRA 1000
DVFKVWDQGA KVFICGSREI GKAVEDVCVR LAIEKAQQNG RDVTEEMARA 1050
WFERSRNERF ATDVFD 1066
Length:1,066
Mass (Da):117,926
Last modified:November 1, 1999 - v1
Checksum:i6B8123698C223DBA
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB030037 Genomic DNA. Translation: BAA82526.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB030037 Genomic DNA. Translation: BAA82526.1 .

3D structure databases

ProteinModelPortali Q9Y8G7.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 1.10.630.10. 1 hit.
1.20.990.10. 1 hit.
3.40.50.360. 1 hit.
InterProi IPR023206. Bifunctional_P450_P450_red.
IPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR008254. Flavodoxin/NO_synth.
IPR029039. Flavoprotein-like.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view ]
Pfami PF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
PF00067. p450. 1 hit.
[Graphical view ]
PIRSFi PIRSF000209. Bifunctional_P450_P450R. 1 hit.
PRINTSi PR00463. EP450I.
PR00385. P450.
SUPFAMi SSF48264. SSF48264. 1 hit.
SSF52218. SSF52218. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
PS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Fusarium oxysporum fatty-acid subterminal hydroxylase (CYP505) is a membrane-bound eukaryotic counterpart of Bacillus megaterium cytochrome P450BM3."
    Kitazume T., Takaya N., Nakayama N., Shoun H.
    J. Biol. Chem. 275:39734-39740(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 82-99; 353-359; 362-380; 518-538; 616-626 AND 1005-1009, SUBCELLULAR LOCATION.
    Strain: MT-811.
  2. "Cytochrome P450foxy, a catalytically self-sufficient fatty acid hydroxylase of the fungus Fusarium oxysporum."
    Nakayama N., Takemae A., Shoun H.
    J. Biochem. 119:435-440(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
    Strain: MT-811.

Entry informationi

Entry nameiC505_FUSOX
AccessioniPrimary (citable) accession number: Q9Y8G7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2002
Last sequence update: November 1, 1999
Last modified: July 9, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing, Multifunctional enzyme

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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