Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q9Y8G7 (C505_FUSOX) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Bifunctional P-450:NADPH-P450 reductase
Alternative name(s):
Cytochrome P450foxy
Fatty acid omega-hydroxylase

Including the following 2 domains:

  1. Cytochrome P450 505
    EC=1.14.14.1
  2. NADPH--cytochrome P450 reductase
    EC=1.6.2.4
Gene names
Name:CYP505
OrganismFusarium oxysporum (Fusarium vascular wilt)
Taxonomic identifier5507 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesHypocreomycetidaeHypocrealesNectriaceaeFusariumFusarium oxysporum species complex

Protein attributes

Sequence length1066 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Functions as a fatty acid monooxygenase. Shows highest activity toward fatty acids with a chain length of 12-14 carbons. The reductase domain is required for electron transfer from NADP to cytochrome P450. Ref.2

Catalytic activity

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

NADPH + n oxidized hemoprotein = NADP+ + n reduced hemoprotein.

Cofactor

Binds 1 FAD By similarity.

Binds 1 FMN By similarity.

Heme group By similarity.

Subcellular location

Membrane; Peripheral membrane protein Ref.1 Ref.2.

Sequence similarities

In the N-terminal section; belongs to the cytochrome P450 family.

Contains 1 FAD-binding FR-type domain.

Contains 1 flavodoxin-like domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10661066Bifunctional P-450:NADPH-P450 reductase
PRO_0000052210

Regions

Domain500 – 641142Flavodoxin-like
Domain676 – 904229FAD-binding FR-type
Region1 – 480480Cytochrome P450
Region481 – 1066586NADPH-P-450 reductase

Sites

Metal binding4071Iron (heme axial ligand) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9Y8G7 [UniParc].

Last modified November 1, 1999. Version 1.
Checksum: 6B8123698C223DBA

FASTA1,066117,926
        10         20         30         40         50         60 
MAESVPIPEP PGYPLIGNLG EFTSNPLSDL NRLADTYGPI FRLRLGAKAP IFVSSNSLIN 

        70         80         90        100        110        120 
EVCDEKRFKK TLKSVLSQVR EGVHDGLFTA FEDEPNWGKA HRILVPAFGP LSIRGMFPEM 

       130        140        150        160        170        180 
HDIATQLCMK FARHGPRTPI DTSDNFTRLA LDTLALCAMD FRFYSYYKEE LHPFIEAMGD 

       190        200        210        220        230        240 
FLTESGNRNR RPPFAPNFLY RAANEKFYGD IALMKSVADE VVAARKASPS DRKDLLAAML 

       250        260        270        280        290        300 
NGVDPQTGEK LSDENITNQL ITFLIAGHET TSGTLSFAMY QLLKNPEAYS KVQKEVDEVV 

       310        320        330        340        350        360 
GRGPVLVEHL TKLPYISAVL RETLRLNSPI TAFGLEAIDD TFLGGKYLVK KGEIVTALLS 

       370        380        390        400        410        420 
RGHVDPVVYG NDADKFIPER MLDDEFARLN KEYPNCWKPF GNGKRACIGR PFAWQESLLA 

       430        440        450        460        470        480 
MVVLFQNFNF TMTDPNYALE IKQTLTIKPD HFYINATLRH GMTPTELEHV LAGNGATSSS 

       490        500        510        520        530        540 
THNIKAAANL DAKAGSGKPM AIFYGSNSGT CEALANRLAS DAPSHGFSAT TVGPLDQAKQ 

       550        560        570        580        590        600 
NLPEDRPVVI VTASYEGQPP SNAAHFIKWM EDLDGNDMEK VSYAVFACGH HDWVETFHRI 

       610        620        630        640        650        660 
PKLVDSTLEK RGGTRLVPMG SADAATSDMF SDFEAWEDIV LWPGLKEKYK ISDEESGGQK 

       670        680        690        700        710        720 
GLLVEVSTPR KTSLRQDVEE ALVVAEKTLT KSGPAKKHIE IQLPSAMTYK AGDYLAILPL 

       730        740        750        760        770        780 
NPKSTVARVF RRFSLAWDSF LKIQSEGPTT LPTNVAISAF DVFSAYVELS QPATKRNILA 

       790        800        810        820        830        840 
LAEATEDKDT IQELERLAGD AYQAEISPKR VSVLDLLEKF PAVALPISSY LAMLPPMRVR 

       850        860        870        880        890        900 
QYSISSSPFA DPSKLTLTYS LLDAPSLSGQ GRHVGVATNF LSHLTAGDKL HVSVRASSEA 

       910        920        930        940        950        960 
FHLPSDAEKT PIICVAAGTG LAPLRGFIQE RAAMLAAGRT LAPALLFFGC RNPEIDDLYA 

       970        980        990       1000       1010       1020 
EEFERWEKMG AVDVRRAYSR ATDKSEGCKY VQDRVYHDRA DVFKVWDQGA KVFICGSREI 

      1030       1040       1050       1060 
GKAVEDVCVR LAIEKAQQNG RDVTEEMARA WFERSRNERF ATDVFD 

« Hide

References

[1]"Fusarium oxysporum fatty-acid subterminal hydroxylase (CYP505) is a membrane-bound eukaryotic counterpart of Bacillus megaterium cytochrome P450BM3."
Kitazume T., Takaya N., Nakayama N., Shoun H.
J. Biol. Chem. 275:39734-39740(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 82-99; 353-359; 362-380; 518-538; 616-626 AND 1005-1009, SUBCELLULAR LOCATION.
Strain: MT-811.
[2]"Cytochrome P450foxy, a catalytically self-sufficient fatty acid hydroxylase of the fungus Fusarium oxysporum."
Nakayama N., Takemae A., Shoun H.
J. Biochem. 119:435-440(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
Strain: MT-811.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB030037 Genomic DNA. Translation: BAA82526.1.

3D structure databases

ProteinModelPortalQ9Y8G7.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D1.10.630.10. 1 hit.
1.20.990.10. 1 hit.
3.40.50.360. 1 hit.
InterProIPR023206. Bifunctional_P450_P450_red.
IPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR008254. Flavodoxin/NO_synth.
IPR029039. Flavoprotein-like.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
PF00067. p450. 1 hit.
[Graphical view]
PIRSFPIRSF000209. Bifunctional_P450_P450R. 1 hit.
PRINTSPR00463. EP450I.
PR00385. P450.
SUPFAMSSF48264. SSF48264. 1 hit.
SSF52218. SSF52218. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEPS00086. CYTOCHROME_P450. 1 hit.
PS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameC505_FUSOX
AccessionPrimary (citable) accession number: Q9Y8G7
Entry history
Integrated into UniProtKB/Swiss-Prot: August 30, 2002
Last sequence update: November 1, 1999
Last modified: July 9, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families