Reviewed, UniProtKB/Swiss-Prot Q9Y8B5 (MPPB_LENED)

Names and origin

Protein names

Recommended name:
    Mitochondrial-processing peptidase subunit beta
    EC=3.4.24.64
Alternative name(s):
    Beta-MPP

Gene names

Name:

mppB

Organism

Lentinula edodes (Shiitake mushroom) (Lentinus edodes)

Taxonomic identifier

5353 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Basidiomycota › Agaricomycotina › Homobasidiomycetes › Agaricomycetidae › Agaricales › Tricholomataceae › Lentinula

Protein attributes

Sequence length	466 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cleaves presequences (transit peptides) from mitochondrial protein precursors.
Catalytic activity	Release of N-terminal transit peptides from precursor proteins imported into the mitochondrion, typically with Arg in position P2.
Cofactor	Divalent cations By similarity.
Subunit structure	Heterodimer of alpha and beta subunits By similarity.
Subcellular location	Mitochondrion matrix By similarity.
Sequence similarities	Belongs to the peptidase M16 family.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential
	Chain	? – 466		Mitochondrial-processing peptidase subunit beta		PRO_0000026781
Sites
	Active site	76	1	Proton acceptor By similarity
	Metal binding	73	1	Zinc By similarity
	Metal binding	77	1	Zinc By similarity
	Metal binding	153	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9Y8B5-1 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: CE3E6E797A1C29D6	FASTA	466	51,163
	10 20 30 40 50 60 MLGRVLKSAA RSQRGLRSFA TTTNLGPFTE ISTLSNGLTV ATESQPHAQT ATVGVWIDAG 70 80 90 100 110 120 SRAETDKTNG TAHFLEHMAF KGTGRRSQHA LELEVENIGA HLNAYTSREQ TVYYAKSFSK 130 140 150 160 170 180 DVPVAVDIIS DILQNSKLES GAIERERDVI LREQQEVDKQ LEEVVFDHLH AVAFQGQPLG 190 200 210 220 230 240 RTILGPKNNI LSIQRDDLAS YIQTNYTADR MVLVGTGGVD HQSLVKLAEK HFSSLPVSAN 250 260 270 280 290 300 PLALGRLSSE RKPTFVGSEA RIRDDELPTA HVAIAVEGVG WSSPDYFPMM VMQSIFGNWD 310 320 330 340 350 360 RSLGASSLLS SRLSHIISSN SLANSFMSFS TSYSDTGLWG IYLVSENLMN LDDTLHFTLK 370 380 390 400 410 420 EWTRMSIAPT EGEVERAKSQ LKAGLLLSLD GTTAVAEDIG RQIVTSGKRM TPAQIENAVD 430 440 450 460 AVSVDDIKRV AQKYLWDKDF ALAAFGNIDG LKDYGRIRND MSSMLY

References

[1]	"Cloning and characterization of the gene encoding beta subunit of mitochondrial processing peptidase from the basidiomycete Lentinula edodes." Zhang M., Xie W., Leung G.S., Deane E.E., Kwan H.S. Gene 206:23-27(1998) [PubMed: 9461410] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF146393 Genomic DNA. Translation: AAD37722.1.
PIR	JC6525.
3D structure databases
HSSP	HSSP built from PDB template 1HR6 based on UniProtKB P10507.
ModBase	Search...
Protein family/group databases
MEROPS	M16.003. M16.980.
Enzyme and pathway databases
BRENDA	3.4.24.64. 260981.
Family and domain databases
InterPro	IPR011237. Pept_M16_core. IPR011765. Pept_M16_N. IPR001431. Pept_M16_Zn_BS. IPR007863. Peptidase_M16_C. [Graphical view]
Gene3D	G3DSA:3.30.830.10. Pept_M16_core. 2 hits.
Pfam	PF00675. Peptidase_M16. 1 hit. PF05193. Peptidase_M16_C. 1 hit. [Graphical view]
PROSITE	PS00143. INSULINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MPPB_LENED

Accession

Primary (citable) accession number: Q9Y8B5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 11, 2003
Last sequence update:	November 1, 1999
Last modified:	June 16, 2009
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)