Q9Y865 (AGALB_ASPNG) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable alpha-galactosidase B
EC=3.2.1.22

Alternative name(s):
Melibiase B

Gene names

Name:

aglB

Organism

Aspergillus niger

Taxonomic identifier

5061 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus

Protein attributes

Sequence length	443 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Hydrolyzes a variety of simple alpha-D-galactoside as well as more complex molecules such as oligosaccharides and polysaccharides By similarity.
Catalytic activity	Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids.
Subcellular location	Secreted Probable.
Induction	Expression on xylan is very high, whereas elevated expression levels are also detected on galactose and xylose. Ref.1
Sequence similarities	Belongs to the glycosyl hydrolase 27 family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cation binding Inferred from electronic annotation. Source: InterPro raffinose alpha-galactosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 16	16	Potential
	Chain	17 – 443	427	Probable alpha-galactosidase B		PRO_0000393218
Regions
	Region	221 – 225	5	Substrate binding By similarity
Sites
	Active site	149	1	Nucleophile By similarity
	Active site	243	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	79	1	N-linked (GlcNAc...) Potential
	Glycosylation	156	1	N-linked (GlcNAc...) Potential
	Glycosylation	176	1	N-linked (GlcNAc...) Potential
	Glycosylation	181	1	N-linked (GlcNAc...) Potential
	Glycosylation	212	1	N-linked (GlcNAc...) Potential
	Glycosylation	232	1	N-linked (GlcNAc...) Potential
	Glycosylation	282	1	N-linked (GlcNAc...) Potential
	Disulfide bond	39 ↔ 71		By similarity
	Disulfide bond	121 ↔ 151		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9Y865 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: 187B7401084D4D99	FASTA	443	48,784
	10 20 30 40 50 60 MRWLLTSSAL LVPAAALVRP DGVGLTPALG WNSWNAYSCD IDADKIVTAA NEVVNLGLKD 70 80 90 100 110 120 LGYEYINIDD CWSVKSGRNT TTKRIIPDPD KFPNGISGVA DQVHALGLKL GIYSSAGLTT 130 140 150 160 170 180 CAGYPASLGY EEIDAQSFAE WGIDYLKYDN CGVPTNLTDQ YTYCVPDSTD GSNYPNGTCV 190 200 210 220 230 240 NLTDAAPQGY DWATSTTAKR YQRMRDALLS VNRTILYSLC DWGQADVNAW GNATGNSWRM 250 260 270 280 290 300 SGDITATWSR IAEIANENSF LMNYANFWGY PDPDMLEVGN GNLTLPENRA HFALWAMMKA 310 320 330 340 350 360 PLIIGTPLDS IDTSHLTILS NKPLLTFHQD AVIGRPAYPY KWGYNPDWTF DPEHPAEYWS 370 380 390 400 410 420 GPTSSGEVFV LMLNSEGEVK TRSAVWEEVP ELKDRGTKKN SKEKKGFKVT DAWTGKDLGC 430 440 VKDKYEVKLQ AHDVAVLVVG GQC

References

[1]

"Differential expression of three alpha-galactosidase genes and a single beta-galactosidase gene from Aspergillus niger." de Vries R.P., van den Broeck H.C., Dekkers E., Manzanares P., de Graaff L.H., Visser J. Appl. Environ. Microbiol. 65:2453-2460(1999) [PubMed: 10347026] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION. Strain: ATCC 9029 / NRRL 3 / CBS 120.49 / DSM 2466 / N400.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y18586 Genomic DNA. Translation: CAB46229.1.
3D structure databases
HSSP	HSSP built from PDB template 1UAS based on UniProtKB Q9FXT4.
ProteinModelPortal	Q9Y865.
SMR	Q9Y865. Positions 17-443.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9Y865.
Protein family/group databases
CAZy	GH27. Glycoside Hydrolase Family 27.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR013780. Glyco_hydro_13_b. IPR002241. Glyco_hydro_27. IPR000111. Glyco_hydro_GHD. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 2 hits. G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit.
Pfam	PF02065. Melibiase. 1 hit. [Graphical view]
PRINTS	PR00740. GLHYDRLASE27.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00512. ALPHA_GALACTOSIDASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AGALB_ASPNG

Accession

Primary (citable) accession number: Q9Y865

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 23, 2010
Last sequence update:	November 1, 1999
Last modified:	July 27, 2011
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program