Reviewed, UniProtKB/Swiss-Prot Q9Y834 (PGLR1_PENOL)

Names and origin

Protein names

Recommended name:
    Polygalacturonase 1
      Short name=PG 1
    EC=3.2.1.15
Alternative name(s):
    Pectinase 1

Gene names

Name:

PG1

Organism

Penicillium olsonii

Taxonomic identifier

99116 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Penicillium

Protein attributes

Sequence length	370 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Random hydrolysis of (1->4)-alpha-D-galactosiduronic linkages in pectate and other galacturonans.
Subcellular location	Secreted.
Sequence similarities	Belongs to the glycosyl hydrolase 28 family. Contains 5 PbH1 repeats.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Secreted
Domain	Repeat Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	polygalacturonase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Potential
	Chain	19 – 370	352	Polygalacturonase 1		PRO_0000024792
Sites
	Active site	209	1	Proton donor By similarity
	Active site	231	1	By similarity
Amino acid modifications
	Glycosylation	248	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9Y834-1 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: 35F97F807C09DC1A	FASTA	370	38,063
	10 20 30 40 50 60 MRTSILSMLA LGAAAVSAAP AAAAAPAELV ERGSSCTFTS ASAAKAGKKS CSSIVLDNIK 70 80 90 100 110 120 VPAGETLDLS NLKSGTKVTF KGETTFGYKE WKGPLIRFSG KNIEVNGASG HVINGGGASW 130 140 150 160 170 180 WDGKGTNGGK TKPKFFYAHS LDDSTITGLN VKNTPVQGFS VQADNLVLDH ITIDNTDGDK 190 200 210 220 230 240 TNGGHNTDAF DVGESTYITI SNANIKNQDD CLAINSGENI IFTGGTCSGG HGLSIGSVGG 250 260 270 280 290 300 RDDNTVKNVT ISDSTVSNSD NGIRIKTIYK AKGEVADVTF SNIELSNIAK YGIVIEQDYE 310 320 330 340 350 360 NGSPTGKPTT GVPITGLTVE KVTGSVKSSG TDVYILCGSG SCSDWTWSGN KVSGGKTSSK 370 CKNVPSGASC

References

[1]	"Cloning and targeted disruption of two polygalacturonase genes in Penicillium olsonii." Wagner F., Kusserow H., Schaefer W. FEMS Microbiol. Lett. 186:293-299(2000) [PubMed: 10802187] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ243521 Genomic DNA. Translation: CAB46908.1.
3D structure databases
HSSP	HSSP built from PDB template 1IB4 based on UniProtKB O74213.
ModBase	Search...
Protein family/group databases
CAZy	GH28. Glycoside Hydrolase Family 28.
Enzyme and pathway databases
BRENDA	3.2.1.15. 276075.
Family and domain databases
InterPro	IPR000743. Glyco_hydro_28. IPR006626. PbH1. IPR012334. Pectin_lyas_fold. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
Pfam	PF00295. Glyco_hydro_28. 1 hit. [Graphical view]
SMART	SM00710. PbH1. 5 hits. [Graphical view]
PROSITE	PS00502. POLYGALACTURONASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PGLR1_PENOL

Accession

Primary (citable) accession number: Q9Y834

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1999
Last modified:	June 16, 2009
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)