Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Homocitrate synthase, mitochondrial

Gene

lys4

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Acetyl-CoA + H2O + 2-oxoglutarate = (R)-2-hydroxybutane-1,2,4-tricarboxylate + CoA.

Pathwayi: L-lysine biosynthesis via AAA pathway

This protein is involved in step 1 of the subpathway that synthesizes L-alpha-aminoadipate from 2-oxoglutarate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Homocitrate synthase, mitochondrial (lys4)
  2. Homocitrate dehydratase, mitochondrial (SPBP4H10.15)
  3. Homoaconitase, mitochondrial (lys2)
  4. Homoisocitrate dehydrogenase (lys12)
  5. no protein annotated in this organism
This subpathway is part of the pathway L-lysine biosynthesis via AAA pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-alpha-aminoadipate from 2-oxoglutarate, the pathway L-lysine biosynthesis via AAA pathway and in Amino-acid biosynthesis.

GO - Molecular functioni

  • homocitrate synthase activity Source: PomBase

GO - Biological processi

  • lysine biosynthetic process Source: PomBase
  • lysine biosynthetic process via aminoadipic acid Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Lysine biosynthesis

Enzyme and pathway databases

BRENDAi2.3.3.14. 5613.
UniPathwayiUPA00033; UER00028.

Names & Taxonomyi

Protein namesi
Recommended name:
Homocitrate synthase, mitochondrial (EC:2.3.3.14)
Gene namesi
Name:lys4
ORF Names:SPBC1105.02c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC1105.02c.
PomBaseiSPBC1105.02c. lys4.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: PomBase
  • mitochondrion Source: PomBase
  • nucleus Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 418Homocitrate synthase, mitochondrialPRO_0000001049
Transit peptidei1 – ?MitochondrionSequence analysis

Proteomic databases

MaxQBiQ9Y823.

Interactioni

Protein-protein interaction databases

BioGridi276365. 44 interactions.
MINTiMINT-4721174.

Structurei

Secondary structure

1
418
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi8 – 103Combined sources
Helixi25 – 295Combined sources
Beta strandi36 – 394Combined sources
Turni41 – 433Combined sources
Helixi44 – 474Combined sources
Helixi55 – 6814Combined sources
Beta strandi71 – 755Combined sources
Helixi82 – 9211Combined sources
Beta strandi97 – 10610Combined sources
Helixi108 – 1169Combined sources
Beta strandi120 – 1278Combined sources
Beta strandi130 – 1367Combined sources
Helixi143 – 15614Combined sources
Turni157 – 1593Combined sources
Beta strandi161 – 1688Combined sources
Helixi169 – 1713Combined sources
Helixi174 – 18714Combined sources
Beta strandi190 – 1967Combined sources
Helixi203 – 21614Combined sources
Beta strandi218 – 2269Combined sources
Helixi232 – 24110Combined sources
Beta strandi246 – 2505Combined sources
Helixi251 – 2533Combined sources
Helixi263 – 27311Combined sources
Helixi275 – 2817Combined sources
Helixi284 – 2863Combined sources
Helixi287 – 29711Combined sources
Turni307 – 3093Combined sources
Turni311 – 3144Combined sources
Beta strandi315 – 3173Combined sources
Helixi320 – 3245Combined sources
Beta strandi325 – 3273Combined sources
Helixi329 – 3313Combined sources
Helixi337 – 3404Combined sources
Beta strandi346 – 3505Combined sources
Helixi355 – 36410Combined sources
Turni374 – 3763Combined sources
Turni379 – 3835Combined sources
Helixi394 – 3996Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3IVSX-ray2.24A/B1-418[»]
3IVTX-ray2.67A/B1-418[»]
3IVUX-ray2.72A/B1-418[»]
3MI3X-ray2.38A/B1-418[»]
ProteinModelPortaliQ9Y823.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9Y823.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

HOGENOMiHOG000046858.
InParanoidiQ9Y823.
KOiK01655.
OMAiASRTDMD.
OrthoDBiEOG76HQ9T.
PhylomeDBiQ9Y823.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR002034. AIPM/Hcit_synth_CS.
IPR013785. Aldolase_TIM.
IPR011872. Homocitrate_synth_fun/arc.
IPR000891. PYR_CT.
[Graphical view]
PfamiPF00682. HMGL-like. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02146. LysS_fung_arch. 1 hit.
PROSITEiPS00815. AIPM_HOMOCIT_SYNTH_1. 1 hit.
PS00816. AIPM_HOMOCIT_SYNTH_2. 1 hit.
PS50991. PYR_CT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9Y823-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVSEANGTE TIKPPMNGNP YGPNPSDFLS RVNNFSIIES TLREGEQFAN
60 70 80 90 100
AFFDTEKKIQ IAKALDNFGV DYIELTSPVA SEQSRQDCEA ICKLGLKCKI
110 120 130 140 150
LTHIRCHMDD ARVAVETGVD GVDVVIGTSQ YLRKYSHGKD MTYIIDSATE
160 170 180 190 200
VINFVKSKGI EVRFSSEDSF RSDLVDLLSL YKAVDKIGVN RVGIADTVGC
210 220 230 240 250
ATPRQVYDLI RTLRGVVSCD IECHFHNDTG MAIANAYCAL EAGATHIDTS
260 270 280 290 300
ILGIGERNGI TPLGALLARM YVTDREYITH KYKLNQLREL ENLVADAVEV
310 320 330 340 350
QIPFNNYITG MCAFTHKAGI HAKAILANPS TYEILKPEDF GMSRYVHVGS
360 370 380 390 400
RLTGWNAIKS RAEQLNLHLT DAQAKELTVR IKKLADVRTL AMDDVDRVLR
410
EYHADLSDAD RITKEASA
Length:418
Mass (Da):46,293
Last modified:November 1, 1999 - v1
Checksum:i55213523E4F46CDE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAB50965.1.
PIRiT39279.
RefSeqiNP_596458.1. NM_001022377.2.

Genome annotation databases

EnsemblFungiiSPBC1105.02c.1; SPBC1105.02c.1:pep; SPBC1105.02c.
GeneIDi2539815.
KEGGispo:SPBC1105.02c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAB50965.1.
PIRiT39279.
RefSeqiNP_596458.1. NM_001022377.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3IVSX-ray2.24A/B1-418[»]
3IVTX-ray2.67A/B1-418[»]
3IVUX-ray2.72A/B1-418[»]
3MI3X-ray2.38A/B1-418[»]
ProteinModelPortaliQ9Y823.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi276365. 44 interactions.
MINTiMINT-4721174.

Proteomic databases

MaxQBiQ9Y823.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPBC1105.02c.1; SPBC1105.02c.1:pep; SPBC1105.02c.
GeneIDi2539815.
KEGGispo:SPBC1105.02c.

Organism-specific databases

EuPathDBiFungiDB:SPBC1105.02c.
PomBaseiSPBC1105.02c. lys4.

Phylogenomic databases

HOGENOMiHOG000046858.
InParanoidiQ9Y823.
KOiK01655.
OMAiASRTDMD.
OrthoDBiEOG76HQ9T.
PhylomeDBiQ9Y823.

Enzyme and pathway databases

UniPathwayiUPA00033; UER00028.
BRENDAi2.3.3.14. 5613.

Miscellaneous databases

EvolutionaryTraceiQ9Y823.
NextBioi20800964.
PROiQ9Y823.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR002034. AIPM/Hcit_synth_CS.
IPR013785. Aldolase_TIM.
IPR011872. Homocitrate_synth_fun/arc.
IPR000891. PYR_CT.
[Graphical view]
PfamiPF00682. HMGL-like. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02146. LysS_fung_arch. 1 hit.
PROSITEiPS00815. AIPM_HOMOCIT_SYNTH_1. 1 hit.
PS00816. AIPM_HOMOCIT_SYNTH_2. 1 hit.
PS50991. PYR_CT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.

Entry informationi

Entry nameiHOSM_SCHPO
AccessioniPrimary (citable) accession number: Q9Y823
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: November 1, 1999
Last modified: November 11, 2015
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.