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Protein

Homocitrate synthase, mitochondrial

Gene

lys4

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Acetyl-CoA + H2O + 2-oxoglutarate = (R)-2-hydroxybutane-1,2,4-tricarboxylate + CoA.

Pathwayi: L-lysine biosynthesis via AAA pathway

This protein is involved in step 1 of the subpathway that synthesizes L-alpha-aminoadipate from 2-oxoglutarate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Homocitrate synthase, mitochondrial (lys4)
  2. Homocitrate dehydratase, mitochondrial (SPBP4H10.15)
  3. Homoaconitase, mitochondrial (lys2)
  4. Homoisocitrate dehydrogenase (lys12)
  5. no protein annotated in this organism
This subpathway is part of the pathway L-lysine biosynthesis via AAA pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-alpha-aminoadipate from 2-oxoglutarate, the pathway L-lysine biosynthesis via AAA pathway and in Amino-acid biosynthesis.

GO - Molecular functioni

  • homocitrate synthase activity Source: PomBase

GO - Biological processi

  • lysine biosynthetic process Source: PomBase
  • lysine biosynthetic process via aminoadipic acid Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Lysine biosynthesis

Enzyme and pathway databases

BRENDAi2.3.3.14. 5613.
UniPathwayiUPA00033; UER00028.

Names & Taxonomyi

Protein namesi
Recommended name:
Homocitrate synthase, mitochondrial (EC:2.3.3.14)
Gene namesi
Name:lys4
ORF Names:SPBC1105.02c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC1105.02c.
PomBaseiSPBC1105.02c. lys4.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: PomBase
  • mitochondrion Source: PomBase
  • nucleus Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000001049? – 418Homocitrate synthase, mitochondrial
Transit peptidei1 – ?MitochondrionSequence analysis

Proteomic databases

MaxQBiQ9Y823.
PRIDEiQ9Y823.

Interactioni

Protein-protein interaction databases

BioGridi276365. 44 interactors.
MINTiMINT-4721174.

Structurei

Secondary structure

1418
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi8 – 10Combined sources3
Helixi25 – 29Combined sources5
Beta strandi36 – 39Combined sources4
Turni41 – 43Combined sources3
Helixi44 – 47Combined sources4
Helixi55 – 68Combined sources14
Beta strandi71 – 75Combined sources5
Helixi82 – 92Combined sources11
Beta strandi97 – 106Combined sources10
Helixi108 – 116Combined sources9
Beta strandi120 – 127Combined sources8
Beta strandi130 – 136Combined sources7
Helixi143 – 156Combined sources14
Turni157 – 159Combined sources3
Beta strandi161 – 168Combined sources8
Helixi169 – 171Combined sources3
Helixi174 – 187Combined sources14
Beta strandi190 – 196Combined sources7
Helixi203 – 216Combined sources14
Beta strandi218 – 226Combined sources9
Helixi232 – 241Combined sources10
Beta strandi246 – 250Combined sources5
Helixi251 – 253Combined sources3
Helixi263 – 273Combined sources11
Helixi275 – 281Combined sources7
Helixi284 – 286Combined sources3
Helixi287 – 297Combined sources11
Turni307 – 309Combined sources3
Turni311 – 314Combined sources4
Beta strandi315 – 317Combined sources3
Helixi320 – 324Combined sources5
Beta strandi325 – 327Combined sources3
Helixi329 – 331Combined sources3
Helixi337 – 340Combined sources4
Beta strandi346 – 350Combined sources5
Helixi355 – 364Combined sources10
Turni374 – 376Combined sources3
Turni379 – 383Combined sources5
Helixi394 – 399Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3IVSX-ray2.24A/B1-418[»]
3IVTX-ray2.67A/B1-418[»]
3IVUX-ray2.72A/B1-418[»]
3MI3X-ray2.38A/B1-418[»]
ProteinModelPortaliQ9Y823.
SMRiQ9Y823.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9Y823.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini35 – 288Pyruvate carboxyltransferasePROSITE-ProRule annotationAdd BLAST254

Sequence similaritiesi

Contains 1 pyruvate carboxyltransferase domain.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

HOGENOMiHOG000046858.
InParanoidiQ9Y823.
KOiK01655.
OMAiASRTDMD.
OrthoDBiEOG092C2OYT.
PhylomeDBiQ9Y823.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR002034. AIPM/Hcit_synth_CS.
IPR013785. Aldolase_TIM.
IPR011872. Homocitrate_synth_fun/arc.
IPR000891. PYR_CT.
[Graphical view]
PfamiPF00682. HMGL-like. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02146. LysS_fung_arch. 1 hit.
PROSITEiPS00815. AIPM_HOMOCIT_SYNTH_1. 1 hit.
PS00816. AIPM_HOMOCIT_SYNTH_2. 1 hit.
PS50991. PYR_CT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9Y823-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVSEANGTE TIKPPMNGNP YGPNPSDFLS RVNNFSIIES TLREGEQFAN
60 70 80 90 100
AFFDTEKKIQ IAKALDNFGV DYIELTSPVA SEQSRQDCEA ICKLGLKCKI
110 120 130 140 150
LTHIRCHMDD ARVAVETGVD GVDVVIGTSQ YLRKYSHGKD MTYIIDSATE
160 170 180 190 200
VINFVKSKGI EVRFSSEDSF RSDLVDLLSL YKAVDKIGVN RVGIADTVGC
210 220 230 240 250
ATPRQVYDLI RTLRGVVSCD IECHFHNDTG MAIANAYCAL EAGATHIDTS
260 270 280 290 300
ILGIGERNGI TPLGALLARM YVTDREYITH KYKLNQLREL ENLVADAVEV
310 320 330 340 350
QIPFNNYITG MCAFTHKAGI HAKAILANPS TYEILKPEDF GMSRYVHVGS
360 370 380 390 400
RLTGWNAIKS RAEQLNLHLT DAQAKELTVR IKKLADVRTL AMDDVDRVLR
410
EYHADLSDAD RITKEASA
Length:418
Mass (Da):46,293
Last modified:November 1, 1999 - v1
Checksum:i55213523E4F46CDE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAB50965.1.
PIRiT39279.
RefSeqiNP_596458.1. NM_001022377.2.

Genome annotation databases

EnsemblFungiiSPBC1105.02c.1; SPBC1105.02c.1:pep; SPBC1105.02c.
GeneIDi2539815.
KEGGispo:SPBC1105.02c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAB50965.1.
PIRiT39279.
RefSeqiNP_596458.1. NM_001022377.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3IVSX-ray2.24A/B1-418[»]
3IVTX-ray2.67A/B1-418[»]
3IVUX-ray2.72A/B1-418[»]
3MI3X-ray2.38A/B1-418[»]
ProteinModelPortaliQ9Y823.
SMRiQ9Y823.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi276365. 44 interactors.
MINTiMINT-4721174.

Proteomic databases

MaxQBiQ9Y823.
PRIDEiQ9Y823.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPBC1105.02c.1; SPBC1105.02c.1:pep; SPBC1105.02c.
GeneIDi2539815.
KEGGispo:SPBC1105.02c.

Organism-specific databases

EuPathDBiFungiDB:SPBC1105.02c.
PomBaseiSPBC1105.02c. lys4.

Phylogenomic databases

HOGENOMiHOG000046858.
InParanoidiQ9Y823.
KOiK01655.
OMAiASRTDMD.
OrthoDBiEOG092C2OYT.
PhylomeDBiQ9Y823.

Enzyme and pathway databases

UniPathwayiUPA00033; UER00028.
BRENDAi2.3.3.14. 5613.

Miscellaneous databases

EvolutionaryTraceiQ9Y823.
PROiQ9Y823.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR002034. AIPM/Hcit_synth_CS.
IPR013785. Aldolase_TIM.
IPR011872. Homocitrate_synth_fun/arc.
IPR000891. PYR_CT.
[Graphical view]
PfamiPF00682. HMGL-like. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02146. LysS_fung_arch. 1 hit.
PROSITEiPS00815. AIPM_HOMOCIT_SYNTH_1. 1 hit.
PS00816. AIPM_HOMOCIT_SYNTH_2. 1 hit.
PS50991. PYR_CT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHOSM_SCHPO
AccessioniPrimary (citable) accession number: Q9Y823
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: November 1, 1999
Last modified: November 30, 2016
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.