Reviewed, UniProtKB/Swiss-Prot Q9Y806 (ERV2_SCHPO)

Names and origin

Protein names

Recommended name:
    FAD-linked sulfhydryl oxidase erv2
    EC=1.8.3.2

Gene names

Name:	erv2
ORF Names:	SPBC146.04

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	192 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	FAD-dependent sulfhydryl oxidase that catalyzes disulfide bond formation in the endoplasmic reticulum lumen By similarity.
Catalytic activity	4 R'C(R)SH + O2 = 2 R'C(R)S-S(R)CR' + 2 H2O.
Cofactor	FAD By similarity.
Subcellular location	Endoplasmic reticulum membrane; Single-pass type III membrane protein; Lumenal side By similarity. Cytoplasm. Nucleus Ref.2.
Sequence similarities	Contains 1 ERV/ALR sulfhydryl oxidase domain.

Ontologies

Keywords
Cellular component	Cytoplasm Endoplasmic reticulum Membrane Nucleus
Domain	Signal-anchor Transmembrane
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
PTM	Disulfide bond
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytosol Ref.2 Inferred from direct assay. Source: GeneDB_SPombe endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Ref.2 Inferred from direct assay. Source: GeneDB_SPombe
Molecular function	thiol oxidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 192	192	FAD-linked sulfhydryl oxidase erv2		PRO_0000339122
Regions
	Topological domain	1 – 8	8	Cytoplasmic Potential
	Transmembrane	9 – 29	21	Signal-anchor for type II/III membrane protein Potential
	Topological domain	30 – 192	163	Lumenal Potential
	Domain	61 – 162	102	ERV/ALR sulfhydryl oxidase
	Region	66 – 74	9	FAD-binding By similarity
	Region	141 – 162	22	FAD-binding By similarity
Amino acid modifications
	Disulfide bond	138 ↔ 155		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9Y806-1 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: A14DCB3F731AB47B	FASTA	192	22,389
	10 20 30 40 50 60 MILNRRIQVI LPTLLILSFI IWIFHSVMVD KDWRLFMPEI KSLPDREGQG RKPIEMMSTK 70 80 90 100 110 120 HDNNTNNLMV NAYWKLIHTV VSNYPNRPTL DERDILRHYL FSSAITMPCG EYSVELQKIL 130 140 150 160 170 180 DVHPPQTSSR KAATTWACKV HNQLNEKMNQ PKTSCDGFNE RYVIGSPTYR ESEAENVPER 190 VQVINEDHDY SG

References

[1]

"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.

[2]

"ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe." Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M. Nat. Biotechnol. 24:841-847(2006) [PubMed: 16823372] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU329671 Genomic DNA. Translation: CAB46757.1.
PIR	T39418.
RefSeq	NP_595393.1.
3D structure databases
HSSP	HSSP built from PDB template 1JR8 based on UniProtKB Q12284.
SMR	Q9Y806. Positions 69-165.
ModBase	Search...
Genome annotation databases
GeneID	2539721.
GenomeReviews	Gene locus erv2 in contig CU329671_GR.
KEGG	spo:SPBC146.04.
NMPDR	fig|4896.1.peg.1259.
Organism-specific databases
GeneDB_Spombe	SPBC146.04.
Phylogenomic databases
eggNOG	fuNOG10324.
OrthoDB	EOG9D80NX.
PhylomeDB	Q9Y806.
Gene expression databases
ArrayExpress	Q9Y806.
Family and domain databases
InterPro	IPR017905. ERV/ALR_sulphydryl_oxidase. IPR006863. Evr1_Alr. [Graphical view]
Gene3D	G3DSA:1.20.120.310. Evr1_Alr. 1 hit.
Pfam	PF04777. Evr1_Alr. 1 hit. [Graphical view]
PROSITE	PS51324. ERV_ALR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ERV2_SCHPO

Accession

Primary (citable) accession number: Q9Y806

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 10, 2008
Last sequence update:	November 1, 1999
Last modified:	February 9, 2010
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)