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Protein

Myosin-1

Gene

myo1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Type-I myosin implicated in the organization of the actin cytoskeleton. Required for proper actin cytoskeleton polarization. At the cell cortex, assembles in patch-like structures together with proteins from the actin-polymerizing machinery and promotes actin assembly. Functions as actin nucleation-promoting factor (NPF) for the Arp2/3 complex. Contributes to proper septation by transporting vesicles containing septal material to the division site and is involved in the formation of sterol-rich membrane domains at the cell division site. Required also for mating.3 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi17 – 24ATPSequence analysis8
Nucleotide bindingi133 – 140ATPSequence analysis8

GO - Molecular functioni

  • actin-dependent ATPase activity Source: PomBase
  • actin filament binding Source: PomBase
  • ATP binding Source: UniProtKB-KW
  • microfilament motor activity Source: PomBase
  • phospholipid binding Source: PomBase

GO - Biological processi

  • actin cortical patch assembly Source: PomBase
  • actin cytoskeleton organization Source: PomBase
  • actin nucleation Source: PomBase
  • endocytosis Source: PomBase
  • establishment or maintenance of cell polarity regulating cell shape Source: PomBase
  • plasma membrane raft distribution Source: PomBase
  • positive regulation of actin nucleation Source: PomBase

Keywordsi

Molecular functionActin-binding, Hydrolase, Motor protein, Myosin
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Myosin-1
Alternative name(s):
Class I unconventional myosin
Type I myosin
Gene namesi
Name:myo1
ORF Names:SPBC146.13c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC146.13c.
PomBaseiSPBC146.13c. myo1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001234791 – 1217Myosin-1Add BLAST1217

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei361Phosphoserine1 Publication1
Modified residuei363Phosphotyrosine1 Publication1
Modified residuei742Phosphoserine1 Publication1
Modified residuei782Phosphoserine1 Publication1
Modified residuei1211Phosphoserine1 Publication1

Post-translational modificationi

Phosphorylation of the TEDS site (Ser-361) is required for the polarization of the actin cytoskeleton. Phosphorylation probably activates the myosin-I ATPase activity (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9Y7Z8.
PRIDEiQ9Y7Z8.

PTM databases

iPTMnetiQ9Y7Z8.

Interactioni

Subunit structurei

Interacts with cam2. Interacts (via SH3 domain) with vrp1.2 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • actin filament binding Source: PomBase

Protein-protein interaction databases

BioGridi276214. 24 interactors.
IntActiQ9Y7Z8. 10 interactors.
MINTiMINT-4720405.
STRINGi4896.SPBC146.13c.1.

Structurei

3D structure databases

ProteinModelPortaliQ9Y7Z8.
SMRiQ9Y7Z8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini40 – 720Myosin motorPROSITE-ProRule annotationAdd BLAST681
Domaini724 – 744IQ 1Add BLAST21
Domaini745 – 770IQ 2Add BLAST26
Domaini778 – 964TH1PROSITE-ProRule annotationAdd BLAST187
Domaini1106 – 1165SH3PROSITE-ProRule annotationAdd BLAST60

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni409 – 491Actin-bindingBy similarityAdd BLAST83

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi963 – 1102Pro-richAdd BLAST140
Compositional biasi1045 – 1050Poly-Thr6

Domaini

The myosin motor domain displays actin-stimulated ATPase activity and generates a mechanochemical force.By similarity
The tail domain participates in molecular interactions that specify the role of the motor domain (By similarity). It is composed of several tail homology (TH) domains, namely a putative phospholipid-binding myosin tail domain (also named TH1), an Ala- and Pro-rich domain (TH2), followed by an SH3 domain and a C-terminal acidic domain (TH3).By similarity

Sequence similaritiesi

Keywords - Domaini

Repeat, SH3 domain

Phylogenomic databases

HOGENOMiHOG000260265.
InParanoidiQ9Y7Z8.
KOiK10356.
OMAiKENWGPW.
OrthoDBiEOG092C0QJU.
PhylomeDBiQ9Y7Z8.

Family and domain databases

CDDicd01378. MYSc_Myo1. 1 hit.
cd11858. SH3_Myosin-I_fungi. 1 hit.
InterProiView protein in InterPro
IPR035535. Fungal_myosin-I_SH3.
IPR001609. Myosin_head_motor_dom.
IPR010926. Myosin_TH1.
IPR036072. MYSc_Myo1.
IPR027417. P-loop_NTPase.
IPR036028. SH3-like_dom_sf.
IPR001452. SH3_domain.
PfamiView protein in Pfam
PF00063. Myosin_head. 1 hit.
PF06017. Myosin_TH1. 1 hit.
PF00018. SH3_1. 1 hit.
PRINTSiPR00193. MYOSINHEAVY.
SMARTiView protein in SMART
SM00242. MYSc. 1 hit.
SM00326. SH3. 1 hit.
SUPFAMiSSF50044. SSF50044. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiView protein in PROSITE
PS51456. MYOSIN_MOTOR. 1 hit.
PS50002. SH3. 1 hit.
PS51757. TH1. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9Y7Z8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAILKRTNRA KAATAAAPNS TGKSNGIKKA VYTSTRKKTV GVDDLTLLSK
60 70 80 90 100
ITDEEINKNL ELRFRNGEIY TYIGHVLISV NPFRDLGIYT MDILKSYQGK
110 120 130 140 150
NRLETSPHVY AIAENAYYQM KSYHENQCII ISGESGAGKT EAAKRIMQYI
160 170 180 190 200
THVSKSVGTE IERVSEIILA TNPLLESFGC AKTLRNNNSS RHGKYLEMIF
210 220 230 240 250
NSGGVPVGAK ITNYLLEKNR IVNQVRNERN FHIFYQFTKS APQKYRDTYG
260 270 280 290 300
IQGPENYVYT SACQCLSVDG ISDEKDFQGT MNAMKVIGIT EPEQDEIFRM
310 320 330 340 350
LSIILWLGNI QFQEGQDGGS VISDKSITEF LGYLIGVPVA AIERALTIRI
360 370 380 390 400
MQTQHGARRG SVYEVPLNPT QALAVRDALS MAIYNCLFDW IVERVNKALV
410 420 430 440 450
TSDNSVSNSI GILDIYGFEI FENNSFEQLC INYVNEKLQQ IFIELTLKTE
460 470 480 490 500
QEEYVREQIA WTPIKYFNNK VVCDLIESKR PPGLFAAMND AIATAHADSA
510 520 530 540 550
AADSAFAQRL NFLSSNPHFE QRQNQFIVKH YAGDVTYSIT GMTDKNKDQL
560 570 580 590 600
ATDILNLIHS SNNEFMKSIF PVAEESNSRR RPPTAGDRIK TSANDLVETL
610 620 630 640 650
MKCQPSYIRT IKPNQTKSPN DYDQQMVLHQ IKYLGLQENI RIRRAGFAYR
660 670 680 690 700
QAFDTFAQRF AVLSGKTSYA GEYTWQGDDK SACEQILKDT NIPSSEYQMG
710 720 730 740 750
TSKVFIKNPE TLFALEDMRD KFWDTMATRI QRAWRSYVRR RSEAAACIQK
760 770 780 790 800
LWNRNKVNME LERVRNEGTK LLQGKKQRRR YSILGSRKFY GDYLSASKPN
810 820 830 840 850
GTLWNTCGLS QNDHVIFSMR CEVLVHKLGR TSKPSPRQLV LTKKNLYLVI
860 870 880 890 900
TKIVDQKLTQ QVEKKFAVSS IDSVGLTNLQ DDWVAIRNKS SQNGDMFLRC
910 920 930 940 950
FFKTEFITTL KRINRNIQVI VGPTIQYCRK PGKVQTVKTA KDETTKDYDY
960 970 980 990 1000
YKSGTIHVGT GLPPTSKSKP FPRLATGGST AAARGPRPVV QNKPAATKPV
1010 1020 1030 1040 1050
SMPAAKSKPA PMANPVSTAQ QTQNRPPAPA MQARPNTTQA AAPVTSTTTT
1060 1070 1080 1090 1100
IKQATTVSAS KPAPSTVTSA ASSPSNISKP SAPVANNVSK PSAVPPPPPP
1110 1120 1130 1140 1150
PPAEVEKKDL YLALYDFAGR SPNEMTIKKD EIIEIVQKEP SGWWLALKNG
1160 1170 1180 1190 1200
AEGWVPATYV TEYKGSTPQT TASSTNVAAQ ANNNASPAEV NNLAGSLADA
1210
LRMRASAVRG SDEEEDW
Length:1,217
Mass (Da):135,710
Last modified:November 1, 1999 - v1
Checksum:i23851048FF5DEF0A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAB46766.1.
PIRiT39427.
RefSeqiNP_595402.1. NM_001021309.2.

Genome annotation databases

EnsemblFungiiSPBC146.13c.1; SPBC146.13c.1:pep; SPBC146.13c.
GeneIDi2539659.
KEGGispo:SPBC146.13c.

Similar proteinsi

Entry informationi

Entry nameiMYO1_SCHPO
AccessioniPrimary (citable) accession number: Q9Y7Z8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 2005
Last sequence update: November 1, 1999
Last modified: November 22, 2017
This is version 138 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families