Q9Y7Y8 (SYQ_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable glutamine--tRNA ligase
EC=6.1.1.18

Alternative name(s):
Glutaminyl-tRNA synthetase
Short name=GlnRS

Gene names

Name:	qrs1
ORF Names:	SPBC342.02

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › Schizosaccharomyces pombe

Protein attributes

Sequence length	811 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-glutaminyl-tRNA(Gln).
Subcellular location	Cytoplasm Ref.2.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cytoplasmic translation Non-traceable author statement. Source: PomBase glutaminyl-tRNA aminoacylation Inferred by curator. Source: PomBase
Cellular_component	cytosol Inferred from direct assay Ref.2. Source: PomBase
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamine-tRNA ligase activity Inferred from sequence or structural similarity. Source: PomBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 811	811	Probable glutamine--tRNA ligase		PRO_0000195865
Regions
	Motif	269 – 279	11	"HIGH" region
	Motif	502 – 506	5	"KMSKS" region
Sites
	Binding site	505	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9Y7Y8 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: AA346369990F94CE	FASTA	811	92,073
	10 20 30 40 50 60 MDQDYEELRA KFTKIGLNET TVKDTLKNKK LSSSLNKVIE ETNVGSSGCD RTIGNLLFTL 70 80 90 100 110 120 ANASLKQKDP KSNAHEAFIA SKIVSGDLKT NLQVNAAITY CKDKDTIDES EFDKETGVGV 130 140 150 160 170 180 VLTPEQIEQL VGDYVAENKS KILEQRYQLL NPSASALRQH ALLKWAPQLE VKQTLDRKFL 190 200 210 220 230 240 ELLGPKTEQD AAAGKKKGAK AKNSKQKTVD SGKAKEQKIV SEQSKKYNMF EEGFLAKLHK 250 260 270 280 290 300 PGGNTQLIPE RMKEHLQATG GGVVTRFPPE PNGYLHIGHS KAIAVNFGFA RYHNGVCYLR 310 320 330 340 350 360 FDDTNPEAEE ERYFESIKDL VAWLGFQPYK ITYSSDYFDK LYELAEELIK RDKAYVCHCT 370 380 390 400 410 420 DAEIKKARGG EERGPRYACV HRDRPIEESL LEFRNMRDGK YQPKEAILRM KQDLSDGNPQ 430 440 450 460 470 480 MWDLIAYRVL NSPHPRTGDK WKIYPTYDFT HCLVDSFENI SHSLCTTEFI LSRVSYEWLC 490 500 510 520 530 540 NALEVYCPAQ REYGRLNVVG TLMSKRKIMK LVKEGYVHGW NDPRLYTLVA LRRRGVPPGA 550 560 570 580 590 600 ILEFVSEVGV TTAVSNIEVA RFENCVRKFL ENSVPRLMFL PDPIKVTLEN LDDSYREQIE 610 620 630 640 650 660 IPFNPKDPSM GSRSAFLTKH IYIDRSDFRE EASSDFFRLT LGQPVGLFRA SHPVVAKRVV 670 680 690 700 710 720 KNDEGEPIEI IAEYDASSSK KPKTFIQWVS RDKESNSPVL IAETRLFNNL FKCDNPAALK 730 740 750 760 770 780 EQELAAQLNP ESEVVLKNSI IEPGIYDLIK SAPWPKTDSS AGVDKAENPE SVRFQAMRVG 790 800 810 YFCLDEDTKK PNHLVLNRIV SLREDSAKNK N

References

[1]

"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.

[2]

"ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe." Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M. Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU329671 Genomic DNA. Translation: CAB46772.1.
PIR	T40275.
RefSeq	NP_596745.1. NM_001023765.2.
3D structure databases
ProteinModelPortal	Q9Y7Y8.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-4720158.
STRING	4896.SPBC342.02-1.
Proteomic databases
PaxDb	Q9Y7Y8.
PRIDE	Q9Y7Y8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPBC342.02.1; SPBC342.02.1:pep; SPBC342.02.
GeneID	2540978.
KEGG	spo:SPBC342.02.
Organism-specific databases
PomBase	SPBC342.02.
Phylogenomic databases
eggNOG	COG0008.
HOGENOM	HOG000259233.
KO	K01886.
OMA	KEVCRFQ.
OrthoDB	EOG4FBN23.
Family and domain databases
Gene3D	1.10.1160.10. 1 hit. 2.40.240.10. 2 hits. 3.40.50.620. 2 hits.
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR004514. Gln-tRNA-synth_Ib. IPR007638. Gln-tRNA-synth_Ib_RNA-bd_2. IPR007639. Gln-tRNA-synth_Ib_RNA-bd_N. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd. IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl. IPR011035. Ribosomal_L25/Gln-tRNA_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
PANTHER	PTHR10119. PTHR10119. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. PF03950. tRNA-synt_1c_C. 1 hit. PF04558. tRNA_synt_1c_R1. 1 hit. PF04557. tRNA_synt_1c_R2. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF50715. Ribosomal_L25rel. 1 hit.
TIGRFAMs	TIGR00440. glnS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20802093.

Entry information

Entry name

SYQ_SCHPO

Accession

Primary (citable) accession number: Q9Y7Y8

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	November 1, 1999
Last modified:	May 29, 2013
This is version 97 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program