Skip Header

Contribute Send feedback
Read comments (0) or add your own

Reviewed, UniProtKB/Swiss-Prot Q9Y7W4 (BUR1_CANAL)

Last modified January 19, 2010. Version 56. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Serine/threonine-protein kinase BUR1
    EC=2.7.11.22
    EC=2.7.11.23
Gene names
Name: CRK1
Synonyms: BUR1
ORF Names: CaO19.3523/3524, CaO19.11006
OrganismCandida albicans (Yeast)
Taxonomic identifier5476 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesmitosporic SaccharomycetalesCandida

Protein attributes

Sequence length746 AA.
Sequence statusComplete.
Protein existenceInferred from homology.

General annotation (Comments)

Function

Serine/threonine-protein kinase involved in transcription regulation. Phosphorylates the UBC2/RAD6 ubiquitin-conjugating enzyme (E2), leading to monoubiquitination of histone H2B and the silencing of telomeric-associated genes. Also required for histone H3 methylation. Necessary for the recovery from pheromone-induced growth arrest in the cell cycle G1 phase By similarity. Required for pseudohyphal growth and virulence in mice. Ref.1

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

Subcellular location

Nucleus By similarity.

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.

Contains 1 protein kinase domain.

Sequence caution

The sequence EAK95842.1 differs from that shown. Reason: Frameshift at position 364. Produces 2 separate ORFs.

The sequence EAK95843.1 differs from that shown. Reason: Frameshift at position 364. Produces 2 separate ORFs.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 746746Serine/threonine-protein kinase BUR1
PRO_0000085679

Regions

Domain44 – 349306Protein kinase
Nucleotide binding50 – 589ATP By similarity

Sites

Active site1791Proton acceptor By similarity
Binding site731ATP By similarity

Natural variations

Natural variant681S → L in allele CaO19.3523/3524.
Natural variant1011K → Q in allele CaO19.3523/3524.
Natural variant1891Q → H in allele CaO19.3523/3524.
Natural variant4121Missing in allele CaO19.3523/3524.
Natural variant4631S → L in allele CaO19.3523/3524.
Natural variant5051S → L in allele CaO19.3523/3524.
Natural variant5161V → I in allele CaO19.3523/3524.
Natural variant5291S → L in allele CaO19.3523/3524.
Natural variant5781R → G in allele CaO19.3523/3524.
Natural variant649 – 6513SLS → LLL in allele CaO19.3523/3524.

Experimental info

Sequence conflict249 – 2502LW → QR in AAD25159. Ref.1
Sequence conflict4931A → G in AAD25159. Ref.1
Sequence conflict5061K → Q in AAD25159. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q9Y7W4-1 [UniParc].

Last modified January 10, 2006. Version 2.
Checksum: 2E5B86F5EA624D85

FASTA74683,396
        10         20         30         40         50         60 
MSVIAGHHVP RSNDQRQYDT PSVPINIAPD SEGHIHEMSR LKDYEVIEKL GQGTFGVVQK 

        70         80         90        100        110        120 
AKSKKDGSLV AIKQLINHSA KEGFPITAMR EITILKQLNH KNILTIQDMI FEEPKMSNRT 

       130        140        150        160        170        180 
DIITMRGSFY TVTPYMSSDL VGLLENPKIK LELGQIKCIM QQLLKGIQYV HNQKFLHRDI 

       190        200        210        220        230        240 
KAANILIGQD GVLKIADFGL ARIYHGNVPR LGMGPGGGEK AYTGLVVTRW YRPPEILLGE 

       250        260        270        280        290        300 
RKYTTAVDLW GIGCVFAELF TGKPILVGKS DSHQAQIVFE LVGSPLTWTD AAKLPNKNEY 

       310        320        330        340        350        360 
SCGLACKRSL EAKFASIMPT EAIDLLSGLL TLDPFKRLNA LDALNHKFFS TDPLPLLPTQ 

       370        380        390        400        410        420 
MPKFEESHEI DKERFKKLKD KEQAVSELKP PTEIRYDNHS ESRYNADHST FGGGGVGGKE 

       430        440        450        460        470        480 
TSFSSGKSDY IDHYEPRARR DHYEPRIRND NKDSNDVRGE FESATRQEQR RRDIQNRLDA 

       490        500        510        520        530        540 
GGMDTYIPKT TTAKLREHSG TESLSKKYDN YQPINVSRGS KSPSPSKLSS ISQSKADLIS 

       550        560        570        580        590        600 
KPSAPKVASR ESSLERKQVS NGIRTTTDVE PPRARARRPT DMFGRPLTSN STQAQPTRNK 

       610        620        630        640        650        660 
SVERPKDLEK PTNGVTEDRN KKPVLEEKKE VVKPNLAIPK IKKSSSLVSL SSRSSTTPVI 

       670        680        690        700        710        720 
SNPSKVTKRA ASSVTPPVLP KKPKISKTSS ESEVSDLEED SDFTGENATV FERFMALEQL 

       730        740 
QKSPVYKRII NEKMRFEKLS GGHKSM 

« Hide

References

« Hide 'large scale' references
[1]"Crk1, a novel Cdc2-related protein kinase, is required for hyphal development and virulence in Candida albicans."
Chen J., Zhou S., Wang Q., Chen X., Pan T., Liu H.
Mol. Cell. Biol. 20:8696-8708(2000) [PubMed: 11073971] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
[2]"The diploid genome sequence of Candida albicans."
Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B., Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W., Scherer S.
Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004) [PubMed: 15123810] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: SC5314.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U92261 Genomic DNA. Translation: AAD25159.1.
AACQ01000094 Genomic DNA. Translation: EAK95843.1. Frameshift.
AACQ01000094 Genomic DNA. Translation: EAK95842.1. Frameshift.
AACQ01000093 Genomic DNA. Translation: EAK95905.1.
RefSeqXP_714882.1.
XP_714883.1.
XP_714945.1.

3D structure databases

SMRQ9Y7W4. Positions 40-355.
ModBaseSearch...

Genome annotation databases

GeneID3643350.
3643413.
3643414.
KEGGcal:CaO19.11006.
cal:CaO19.3523.
cal:CaO19.3524.

Organism-specific databases

CGDCAL0073553. CRK1.

Phylogenomic databases

OrthoDBEOG96T4J6.
PhylomeDBQ9Y7W4.

Enzyme and pathway databases

BRENDA2.7.11.22. 1124.
2.7.11.23. 1124.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_cat_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR017442. Se/Thr_prot_kinase-like_dom.
IPR008271. Ser/Thr_prot_kinase_AS.
IPR002290. Ser/Thr_prot_kinase_dom.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBUR1_CANAL
AccessionPrimary (citable) accession number: Q9Y7W4
Secondary accession number(s): Q59ZJ5, Q59ZJ6, Q59ZR0
Entry history
Integrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: January 10, 2006
Last modified: January 19, 2010
This is version 56 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectFPAP (Fungal Proteome Annotation Project)

Relevant documents

Candida albicans

Candida albicans: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents