Q9Y7W4 (BUR1_CANAL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine/threonine-protein kinase BUR1
EC=2.7.11.22
EC=2.7.11.23

Gene names

Name:	CRK1
Synonyms:	BUR1
ORF Names:	CaO19.11006, CaO19.3523/3524

Organism

Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast) [Reference proteome]

Taxonomic identifier

237561 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › mitosporic Saccharomycetales › Candida › Candida albicans

Protein attributes

Sequence length	746 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Serine/threonine-protein kinase involved in transcription regulation. Phosphorylates the UBC2/RAD6 ubiquitin-conjugating enzyme (E2), leading to monoubiquitination of histone H2B and the silencing of telomeric-associated genes. Also required for histone H3 methylation. Necessary for the recovery from pheromone-induced growth arrest in the cell cycle G1 phase By similarity. Required for pseudohyphal growth and virulence in mice. Ref.1
Catalytic activity	ATP + a protein = ADP + a phosphoprotein. ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. Contains 1 protein kinase domain.
Sequence caution	The sequence EAK95842.1 differs from that shown. Reason: Frameshift at position 364. Produces 2 separate ORFs. The sequence EAK95843.1 differs from that shown. Reason: Frameshift at position 364. Produces 2 separate ORFs.

Ontologies

Keywords
Cellular component	Nucleus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell cycle Inferred from electronic annotation. Source: GOC cellular response to pH Inferred from mutant phenotype PubMed 12114968. Source: CGD filamentous growth of a population of unicellular organisms in response to pH Inferred from mutant phenotype PubMed 12114968. Source: CGD flocculation Inferred from mutant phenotype PubMed 12114968. Source: CGD negative regulation of filamentous growth of a population of unicellular organisms in response to pH Inferred from mutant phenotype PubMed 12114968. Source: CGD negative regulation of flocculation Inferred from mutant phenotype PubMed 12114968. Source: CGD pathogenesis Inferred from mutant phenotype Ref.1. Source: CGD positive regulation of filamentous growth of a population of unicellular organisms Inferred from mutant phenotype Ref.1. Source: CGD
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW MAP kinase activity Inferred from sequence or structural similarity PubMed 12114967. Source: CGD RNA polymerase II carboxy-terminal domain kinase activity Inferred from electronic annotation. Source: EC cyclin-dependent protein serine/threonine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 746	746	Serine/threonine-protein kinase BUR1		PRO_0000085679
Regions
	Domain	44 – 349	306	Protein kinase
	Nucleotide binding	50 – 58	9	ATP By similarity
Sites
	Active site	179	1	Proton acceptor By similarity
	Binding site	73	1	ATP By similarity
Natural variations
	Natural variant	68	1	S → L in allele CaO19.3523/3524.
	Natural variant	101	1	K → Q in allele CaO19.3523/3524.
	Natural variant	189	1	Q → H in allele CaO19.3523/3524.
	Natural variant	412	1	Missing in allele CaO19.3523/3524.
	Natural variant	463	1	S → L in allele CaO19.3523/3524.
	Natural variant	505	1	S → L in allele CaO19.3523/3524.
	Natural variant	516	1	V → I in allele CaO19.3523/3524.
	Natural variant	529	1	S → L in allele CaO19.3523/3524.
	Natural variant	578	1	R → G in allele CaO19.3523/3524.
	Natural variant	649 – 651	3	SLS → LLL in allele CaO19.3523/3524.
Experimental info
	Sequence conflict	249 – 250	2	LW → QR in AAD25159. Ref.1
	Sequence conflict	493	1	A → G in AAD25159. Ref.1
	Sequence conflict	506	1	K → Q in AAD25159. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9Y7W4 [UniParc]. Last modified January 10, 2006. Version 2. Checksum: 2E5B86F5EA624D85	FASTA	746	83,396
	10 20 30 40 50 60 MSVIAGHHVP RSNDQRQYDT PSVPINIAPD SEGHIHEMSR LKDYEVIEKL GQGTFGVVQK 70 80 90 100 110 120 AKSKKDGSLV AIKQLINHSA KEGFPITAMR EITILKQLNH KNILTIQDMI FEEPKMSNRT 130 140 150 160 170 180 DIITMRGSFY TVTPYMSSDL VGLLENPKIK LELGQIKCIM QQLLKGIQYV HNQKFLHRDI 190 200 210 220 230 240 KAANILIGQD GVLKIADFGL ARIYHGNVPR LGMGPGGGEK AYTGLVVTRW YRPPEILLGE 250 260 270 280 290 300 RKYTTAVDLW GIGCVFAELF TGKPILVGKS DSHQAQIVFE LVGSPLTWTD AAKLPNKNEY 310 320 330 340 350 360 SCGLACKRSL EAKFASIMPT EAIDLLSGLL TLDPFKRLNA LDALNHKFFS TDPLPLLPTQ 370 380 390 400 410 420 MPKFEESHEI DKERFKKLKD KEQAVSELKP PTEIRYDNHS ESRYNADHST FGGGGVGGKE 430 440 450 460 470 480 TSFSSGKSDY IDHYEPRARR DHYEPRIRND NKDSNDVRGE FESATRQEQR RRDIQNRLDA 490 500 510 520 530 540 GGMDTYIPKT TTAKLREHSG TESLSKKYDN YQPINVSRGS KSPSPSKLSS ISQSKADLIS 550 560 570 580 590 600 KPSAPKVASR ESSLERKQVS NGIRTTTDVE PPRARARRPT DMFGRPLTSN STQAQPTRNK 610 620 630 640 650 660 SVERPKDLEK PTNGVTEDRN KKPVLEEKKE VVKPNLAIPK IKKSSSLVSL SSRSSTTPVI 670 680 690 700 710 720 SNPSKVTKRA ASSVTPPVLP KKPKISKTSS ESEVSDLEED SDFTGENATV FERFMALEQL 730 740 QKSPVYKRII NEKMRFEKLS GGHKSM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Crk1, a novel Cdc2-related protein kinase, is required for hyphal development and virulence in Candida albicans." Chen J., Zhou S., Wang Q., Chen X., Pan T., Liu H. Mol. Cell. Biol. 20:8696-8708(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
[2]	"The diploid genome sequence of Candida albicans." Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B., Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W., Scherer S. Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SC5314 / ATCC MYA-2876.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U92261 Genomic DNA. Translation: AAD25159.1. AACQ01000094 Genomic DNA. Translation: EAK95843.1. Frameshift. AACQ01000094 Genomic DNA. Translation: EAK95842.1. Frameshift. AACQ01000093 Genomic DNA. Translation: EAK95905.1.
RefSeq	XP_714882.1. XM_709789.1. XP_714883.1. XM_709790.1. XP_714945.1. XM_709852.1.
3D structure databases
ProteinModelPortal	Q9Y7W4.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-1173580.
STRING	5476.CAL0005839.
Proteomic databases
PRIDE	Q9Y7W4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3643350. 3643413. 3643414.
KEGG	cal:CaO19.11006. cal:CaO19.3523. cal:CaO19.3524.
Organism-specific databases
CGD	CAL0073553. CRK1.
Phylogenomic databases
eggNOG	COG0515.
KO	K15562.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR002290. Ser/Thr_dual-sp_kinase_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BUR1_CANAL

Accession

Primary (citable) accession number: Q9Y7W4
Secondary accession number(s): Q59ZJ5, Q59ZJ6, Q59ZR0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 10, 2006
Last sequence update:	January 10, 2006
Last modified:	May 1, 2013
This is version 81 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program