Serine/threonine-protein kinase BUR1 - Candida albicans (Yeast)

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Reviewed, UniProtKB/Swiss-Prot Q9Y7W4 (BUR1_CANAL)

Last modified January 19, 2010. Version 56. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Serine/threonine-protein kinase BUR1
    EC=2.7.11.22
    EC=2.7.11.23

Gene names

Name:	CRK1
Synonyms:	BUR1
ORF Names:	CaO19.3523/3524, CaO19.11006

Organism

Candida albicans (Yeast)

Taxonomic identifier

5476 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › mitosporic Saccharomycetales › Candida

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Protein attributes

Sequence length	746 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

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General annotation (Comments)

Function	Serine/threonine-protein kinase involved in transcription regulation. Phosphorylates the UBC2/RAD6 ubiquitin-conjugating enzyme (E2), leading to monoubiquitination of histone H2B and the silencing of telomeric-associated genes. Also required for histone H3 methylation. Necessary for the recovery from pheromone-induced growth arrest in the cell cycle G1 phase By similarity. Required for pseudohyphal growth and virulence in mice. Ref.1
Catalytic activity	ATP + a protein = ADP + a phosphoprotein. ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. Contains 1 protein kinase domain.
Sequence caution	The sequence EAK95842.1 differs from that shown. Reason: Frameshift at position 364. Produces 2 separate ORFs. The sequence EAK95843.1 differs from that shown. Reason: Frameshift at position 364. Produces 2 separate ORFs.

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Ontologies

Keywords
Cellular component	Nucleus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA polymerase II carboxy-terminal domain kinase activity Inferred from electronic annotation. Source: EC cyclin-dependent protein kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 746

746

Serine/threonine-protein kinase BUR1

PRO_0000085679

Regions

Domain

44 – 349

306

Protein kinase

Nucleotide binding

50 – 58

ATP By similarity

Sites

Active site

179

Proton acceptor By similarity

Binding site

ATP By similarity

Natural variations

Natural variant

S → L in allele CaO19.3523/3524.

Natural variant

101

K → Q in allele CaO19.3523/3524.

Natural variant

189

Q → H in allele CaO19.3523/3524.

Natural variant

412

Missing in allele CaO19.3523/3524.

Natural variant

463

S → L in allele CaO19.3523/3524.

Natural variant

505

S → L in allele CaO19.3523/3524.

Natural variant

516

V → I in allele CaO19.3523/3524.

Natural variant

529

S → L in allele CaO19.3523/3524.

Natural variant

578

R → G in allele CaO19.3523/3524.

Natural variant

649 – 651

SLS → LLL in allele CaO19.3523/3524.

Experimental info

Sequence conflict

249 – 250

LW → QR in AAD25159. Ref.1

Sequence conflict

493

A → G in AAD25159. Ref.1

Sequence conflict

506

K → Q in AAD25159. Ref.1

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Sequences

Sequence

Length

Mass (Da)

Tools

Q9Y7W4-1 [UniParc].

Last modified January 10, 2006. Version 2.
Checksum: 2E5B86F5EA624D85
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FASTA

746

83,396

        10         20         30         40         50         60 
MSVIAGHHVP RSNDQRQYDT PSVPINIAPD SEGHIHEMSR LKDYEVIEKL GQGTFGVVQK 

        70         80         90        100        110        120 
AKSKKDGSLV AIKQLINHSA KEGFPITAMR EITILKQLNH KNILTIQDMI FEEPKMSNRT 

       130        140        150        160        170        180 
DIITMRGSFY TVTPYMSSDL VGLLENPKIK LELGQIKCIM QQLLKGIQYV HNQKFLHRDI 

       190        200        210        220        230        240 
KAANILIGQD GVLKIADFGL ARIYHGNVPR LGMGPGGGEK AYTGLVVTRW YRPPEILLGE 

       250        260        270        280        290        300 
RKYTTAVDLW GIGCVFAELF TGKPILVGKS DSHQAQIVFE LVGSPLTWTD AAKLPNKNEY 

       310        320        330        340        350        360 
SCGLACKRSL EAKFASIMPT EAIDLLSGLL TLDPFKRLNA LDALNHKFFS TDPLPLLPTQ 

       370        380        390        400        410        420 
MPKFEESHEI DKERFKKLKD KEQAVSELKP PTEIRYDNHS ESRYNADHST FGGGGVGGKE 

       430        440        450        460        470        480 
TSFSSGKSDY IDHYEPRARR DHYEPRIRND NKDSNDVRGE FESATRQEQR RRDIQNRLDA 

       490        500        510        520        530        540 
GGMDTYIPKT TTAKLREHSG TESLSKKYDN YQPINVSRGS KSPSPSKLSS ISQSKADLIS 

       550        560        570        580        590        600 
KPSAPKVASR ESSLERKQVS NGIRTTTDVE PPRARARRPT DMFGRPLTSN STQAQPTRNK 

       610        620        630        640        650        660 
SVERPKDLEK PTNGVTEDRN KKPVLEEKKE VVKPNLAIPK IKKSSSLVSL SSRSSTTPVI 

       670        680        690        700        710        720 
SNPSKVTKRA ASSVTPPVLP KKPKISKTSS ESEVSDLEED SDFTGENATV FERFMALEQL 

       730        740 
QKSPVYKRII NEKMRFEKLS GGHKSM

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Crk1, a novel Cdc2-related protein kinase, is required for hyphal development and virulence in Candida albicans." Chen J., Zhou S., Wang Q., Chen X., Pan T., Liu H. Mol. Cell. Biol. 20:8696-8708(2000) [PubMed: 11073971] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
[2]	"The diploid genome sequence of Candida albicans." Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B., Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W., Scherer S. Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004) [PubMed: 15123810] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SC5314.

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Cross-references

Sequence databases
EMBL GenBank DDBJ	U92261 Genomic DNA. Translation: AAD25159.1. AACQ01000094 Genomic DNA. Translation: EAK95843.1. Frameshift. AACQ01000094 Genomic DNA. Translation: EAK95842.1. Frameshift. AACQ01000093 Genomic DNA. Translation: EAK95905.1.
RefSeq	XP_714882.1. XP_714883.1. XP_714945.1.
3D structure databases
SMR	Q9Y7W4. Positions 40-355.
ModBase	Search...
Genome annotation databases
GeneID	3643350. 3643413. 3643414.
KEGG	cal:CaO19.11006. cal:CaO19.3523. cal:CaO19.3524.
Organism-specific databases
CGD	CAL0073553. CRK1.
Phylogenomic databases
OrthoDB	EOG96T4J6.
PhylomeDB	Q9Y7W4.
Enzyme and pathway databases
BRENDA	2.7.11.22. 1124. 2.7.11.23. 1124.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_prot_kinase-like_dom. IPR008271. Ser/Thr_prot_kinase_AS. IPR002290. Ser/Thr_prot_kinase_dom. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

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Entry information

Entry name

BUR1_CANAL

Accession

Primary (citable) accession number: Q9Y7W4
Secondary accession number(s): Q59ZJ5, Q59ZJ6, Q59ZR0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 10, 2006
Last sequence update:	January 10, 2006
Last modified:	January 19, 2010
This is version 56 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)

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Relevant documents

Candida albicans

Candida albicans: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Natural variations

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents