Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Pre-mRNA-splicing ATP-dependent RNA helicase prp28

Gene

prp28

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

ATP-dependent RNA helicase involved in mRNA splicing. May destabilize the U1/5'-splice site duplex to permit an effective competition for the 5'-splice site by the U6 snRNA, resulting in the switch between U1 and U6 at the 5'-splice site. May also act to unwind the U4/U6 base-pairing interaction in the U4/U6/U5 snRNP, facilitating the first covalent step of splicing (By similarity).By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi293 – 3008ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-SPO-72165. mRNA Splicing - Minor Pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Pre-mRNA-splicing ATP-dependent RNA helicase prp28 (EC:3.6.4.13)
Gene namesi
Name:prp28
ORF Names:SPCC63.11
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:SPCC63.11.
PomBaseiSPCC63.11. prp28.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • nucleus Source: PomBase
  • spliceosomal complex Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 662662Pre-mRNA-splicing ATP-dependent RNA helicase prp28PRO_0000232377Add
BLAST

Proteomic databases

MaxQBiQ9Y7T7.
PRIDEiQ9Y7T7.

Interactioni

Subunit structurei

Component of the U5 snRNP complex.By similarity

Protein-protein interaction databases

BioGridi275312. 2 interactions.
MINTiMINT-4719023.

Structurei

3D structure databases

ProteinModelPortaliQ9Y7T7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini280 – 477198Helicase ATP-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini488 – 651164Helicase C-terminalPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi249 – 27729Q motifAdd
BLAST
Motifi406 – 4094DEAD box

Domaini

The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.

Sequence similaritiesi

Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000268796.
InParanoidiQ9Y7T7.
KOiK12858.
OMAiNPIYKEK.
OrthoDBiEOG7XH70W.
PhylomeDBiQ9Y7T7.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9Y7T7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTAQDSIPSL EQLVQQKRVK EEKAARPKFL SKAERARLAL ERRQKEVEEA
60 70 80 90 100
KAKQNDKLLD LRKRTFTNHL ENNELADDEK KSQVSSVSSN NSGTESSATD
110 120 130 140 150
EAFSMTIRQR YMGIKPPVVK KRRRNADKKF VFDWDATDDT MKDAETSASP
160 170 180 190 200
EATIAVFGRG KLGGFDDQSI RKAKSNSGLI QRLLQGTEQD KARAHELIQL
210 220 230 240 250
QEKRAKKIDW DDVPWREKPL EAMKPRDWRI LKEDYNISIK GDDLPNPLRN
260 270 280 290 300
WEEAGLPSEM LKVLKKVNYK EPSSIQRAAI PVLLQRKDLI GIAETGSGKT
310 320 330 340 350
AAFIIPLIIA ISKLPPLTES NMHLGPYAVV LAPTRELAQQ IQVEGNKFAE
360 370 380 390 400
PLGFRCVSVV GGHAFEEQSF QMSQGAHIVV ATPGRLLDCL ERRLFVLSQC
410 420 430 440 450
TYVVMDEADR MLDMGFEDDV NKILSSLPSS NASEKDGSIL ATANSSSSRR
460 470 480 490 500
QTIMFSATLP PRVANLAKSY LIEPVMLTIG NIGQAVDRVE QRVEMISDDS
510 520 530 540 550
KKWRRVEEIL ESNRFSPPII IFVNLKRNIE AIAKQLNAIG WHAVTLHGSK
560 570 580 590 600
SQEQRERAIE QLRNKTADIL VATDIAGRGI DIPNVSLVLN YNMAKSIEDY
610 620 630 640 650
THRIGRTGRA GKSGTAITFL GPEDTDVYYD LRVLLSRSAK AHIPDELRNH
660
EAAFVRHAAI TQ
Length:662
Mass (Da):74,224
Last modified:November 1, 1999 - v1
Checksum:i093E79C849CEEE9B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329672 Genomic DNA. Translation: CAB40015.1.
PIRiT41512.
RefSeqiNP_587984.1. NM_001022975.2.

Genome annotation databases

EnsemblFungiiSPCC63.11.1; SPCC63.11.1:pep; SPCC63.11.
GeneIDi2538728.
KEGGispo:SPCC63.11.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329672 Genomic DNA. Translation: CAB40015.1.
PIRiT41512.
RefSeqiNP_587984.1. NM_001022975.2.

3D structure databases

ProteinModelPortaliQ9Y7T7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi275312. 2 interactions.
MINTiMINT-4719023.

Proteomic databases

MaxQBiQ9Y7T7.
PRIDEiQ9Y7T7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPCC63.11.1; SPCC63.11.1:pep; SPCC63.11.
GeneIDi2538728.
KEGGispo:SPCC63.11.

Organism-specific databases

EuPathDBiFungiDB:SPCC63.11.
PomBaseiSPCC63.11. prp28.

Phylogenomic databases

HOGENOMiHOG000268796.
InParanoidiQ9Y7T7.
KOiK12858.
OMAiNPIYKEK.
OrthoDBiEOG7XH70W.
PhylomeDBiQ9Y7T7.

Enzyme and pathway databases

ReactomeiR-SPO-72165. mRNA Splicing - Minor Pathway.

Miscellaneous databases

NextBioi20799913.
PROiQ9Y7T7.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.

Entry informationi

Entry nameiPRP28_SCHPO
AccessioniPrimary (citable) accession number: Q9Y7T7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: November 1, 1999
Last modified: May 11, 2016
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.