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Protein

Alpha-amylase 3

Gene

aah3

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Has a role in cell wall biosynthesis where it is involved in maintaining cell wall strength and shape.1 Publication

Catalytic activityi

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.

Cofactori

Ca2+By similarityNote: Binds 2 calcium ions per subunit. Calcium is inhibitory at high concentrations.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei56SubstrateBy similarity1
Binding sitei105SubstrateBy similarity1
Metal bindingi143Calcium 1By similarity1
Metal bindingi198Calcium 1By similarity1
Binding sitei227SubstrateBy similarity1
Active sitei229Nucleophile1 Publication1
Metal bindingi229Calcium 2By similarity1
Active sitei253Proton donor1 Publication1
Metal bindingi253Calcium 2By similarity1
Binding sitei322SubstrateBy similarity1
Sitei322Transition state stabilizerBy similarity1
Binding sitei369SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

  • ascospore wall assembly Source: PomBase
  • ascospore wall biogenesis Source: PomBase
  • carbohydrate catabolic process Source: InterPro
  • fungal-type cell wall biogenesis Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism

Keywords - Ligandi

Calcium, Metal-binding

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-amylase 3 (EC:3.2.1.1)
Alternative name(s):
1,4-alpha-D-glucan glucanohydrolase
Gene namesi
Name:aah3
ORF Names:SPCC63.02c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:SPCC63.02c.
PomBaseiSPCC63.02c. aah3.

Subcellular locationi

GO - Cellular componenti

  • anchored component of external side of plasma membrane Source: PomBase
  • anchored component of plasma membrane Source: PomBase
  • cell wall Source: PomBase
  • endoplasmic reticulum Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi229D → A: No activity. 1 Publication1
Mutagenesisi253E → A: No activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
ChainiPRO_000000135822 – 538Alpha-amylase 3Add BLAST517
PropeptideiPRO_0000255453539 – 564Removed in mature formSequence analysisAdd BLAST26

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi51 ↔ 59By similarity
Disulfide bondi172 ↔ 188By similarity
Glycosylationi181N-linked (GlcNAc...)Sequence analysis1
Glycosylationi235N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi263 ↔ 306By similarity
Glycosylationi282N-linked (GlcNAc...)Sequence analysis1
Glycosylationi305N-linked (GlcNAc...)Sequence analysis1
Glycosylationi438N-linked (GlcNAc...)Sequence analysis1
Glycosylationi447N-linked (GlcNAc...)Sequence analysis1
Glycosylationi498N-linked (GlcNAc...)Sequence analysis1
Lipidationi538GPI-anchor amidated serineSequence analysis1

Post-translational modificationi

N-glycosylated.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

MaxQBiQ9Y7S9.
PRIDEiQ9Y7S9.

Interactioni

Protein-protein interaction databases

BioGridi275980. 2 interactors.
MINTiMINT-4718683.

Structurei

3D structure databases

ProteinModelPortaliQ9Y7S9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni232 – 233Substrate bindingBy similarity2

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi537 – 540Poly-Ser4

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000165530.
InParanoidiQ9Y7S9.
KOiK01176.
OMAiENINRAP.
OrthoDBiEOG092C1HLH.
PhylomeDBiQ9Y7S9.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR015340. A_amylase_DUF1966_C.
IPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF09260. DUF1966. 1 hit.
[Graphical view]
SMARTiSM00642. Aamy. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9Y7S9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFGVYFVLLF LSSALIHVAN AGSNAEWRKR IIYQILTDRF AVDDGSTDNP
60 70 80 90 100
CDPDANQYCG GTWKGIENKL DYIEDMGFNA IWISPIDKNI EGDIDGAGYA
110 120 130 140 150
YHGYWNTDYE SLNEHFGTED DLVSLITAAH KAGIWVMLDS IVNSMALAPP
160 170 180 190 200
LADADYSSLN PFNKESYFHP YCLIDWDITD NETNVMDCWQ DSGVLLADLD
210 220 230 240 250
VESSDVSSYL SDHFKSLISK YDFDGLRIDA VKMMNYTFFP DFVDATGVYS
260 270 280 290 300
VGEVFSYDPD TMCSYMSVLP GVTNYFLQLY INFSFTATGA GFTLIPTYQE
310 320 330 340 350
VMASNCSKYD STLMLTFIEN HDLYRFPYYT SDQSQIMGAL SFVLIWDGIP
360 370 380 390 400
SIFYGQEQGF NGGEDPANRP ALWLTDYDQS NPYYTVIKTM VAFRKFVITQ
410 420 430 440 450
DPDWVTSTYQ SIESAVDHYV GQKNDVLVMF NNMGVTNNLT IYEVETNYTA
460 470 480 490 500
NEVVSDVFGH RTLTVGADKT LTASMTNGYP LIMYPHSKMS GFTLPTVNRT
510 520 530 540 550
VMPSTSATAT TTVYTSYYSP SYSARSFTGT GSIFTISSSS RLILSFKTLV
560
FGLGVTAMLF VLFF
Length:564
Mass (Da):63,206
Last modified:November 1, 1999 - v1
Checksum:i1229FD4AEC702FD0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329672 Genomic DNA. Translation: CAB40006.1.
PIRiT41503.
RefSeqiNP_587976.1. NM_001022967.2.

Genome annotation databases

EnsemblFungiiSPCC63.02c.1; SPCC63.02c.1:pep; SPCC63.02c.
GeneIDi2539415.
KEGGispo:SPCC63.02c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329672 Genomic DNA. Translation: CAB40006.1.
PIRiT41503.
RefSeqiNP_587976.1. NM_001022967.2.

3D structure databases

ProteinModelPortaliQ9Y7S9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi275980. 2 interactors.
MINTiMINT-4718683.

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

Proteomic databases

MaxQBiQ9Y7S9.
PRIDEiQ9Y7S9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPCC63.02c.1; SPCC63.02c.1:pep; SPCC63.02c.
GeneIDi2539415.
KEGGispo:SPCC63.02c.

Organism-specific databases

EuPathDBiFungiDB:SPCC63.02c.
PomBaseiSPCC63.02c. aah3.

Phylogenomic databases

HOGENOMiHOG000165530.
InParanoidiQ9Y7S9.
KOiK01176.
OMAiENINRAP.
OrthoDBiEOG092C1HLH.
PhylomeDBiQ9Y7S9.

Miscellaneous databases

PROiQ9Y7S9.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR015340. A_amylase_DUF1966_C.
IPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF09260. DUF1966. 1 hit.
[Graphical view]
SMARTiSM00642. Aamy. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiAMY3_SCHPO
AccessioniPrimary (citable) accession number: Q9Y7S9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 2002
Last sequence update: November 1, 1999
Last modified: September 7, 2016
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.