Q9Y7P0 (RISA_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Riboflavin synthase
Short name=RS
EC=2.5.1.9

Gene names

Name:	rib5
ORF Names:	SPCC1450.13c

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › Schizosaccharomyces pombe

Protein attributes

Sequence length	208 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the dismutation of two molecules of 6,7-dimethyl-8-ribityllumazine, resulting in the formation of riboflavin and 5-amino-6-(D-ribitylamino)uracil. Ref.1
Catalytic activity	2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine.
Pathway	Cofactor biosynthesis; riboflavin biosynthesis; riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil: step 2/2.
Subunit structure	Homotrimer. Ref.1
Sequence similarities	Contains 2 lumazine-binding repeats.

Ontologies

Keywords
Biological process	Riboflavin biosynthesis
Domain	Repeat
Molecular function	Transferase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular response to stress Inferred from expression pattern PubMed 12529438. Source: PomBase riboflavin biosynthetic process Inferred from direct assay Ref.1. Source: PomBase
Cellular_component	cytosol Inferred from direct assay PubMed 16823372. Source: PomBase nucleus Inferred from direct assay PubMed 16823372. Source: PomBase
Molecular_function	oxidoreductase activity Inferred from electronic annotation. Source: InterPro riboflavin synthase activity Inferred from direct assay Ref.1. Source: PomBase
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 208

208

Riboflavin synthase

PRO_0000068173

Regions

Repeat

1 – 97

97

Lumazine-binding 1

Repeat

98 – 195

98

Lumazine-binding 2

Region

81 – 85

5

Lumazine binding Probable

Region

179 – 183

5

Lumazine binding Probable

Secondary structure

1

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208

Helix Strand Turn

Details...

Beta strand

8 – 18

11

Turn

19 – 21

3

Beta strand

22 – 28

7

Helix

30 – 32

3

Beta strand

41 – 44

4

Beta strand

47 – 54

8

Beta strand

56 – 63

8

Helix

65 – 70

6

Helix

73 – 75

3

Beta strand

81 – 86

6

Beta strand

94 – 96

3

Beta strand

105 – 115

11

Beta strand

118 – 127

10

Helix

128 – 133

6

Beta strand

139 – 142

4

Beta strand

145 – 152

8

Beta strand

157 – 161

5

Helix

163 – 166

4

Helix

170 – 173

4

Beta strand

179 – 184

6

Helix

187 – 198

12

Turn

199 – 201

3

Sequences

	Sequence		Length	Mass (Da)
	Q9Y7P0 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: 59C5065DDD4C7B8B	FASTA	208	22,861
	10 20 30 40 50 60 MFTGLVEAIG VVKDVQGTID NGFAMKIEAP QILDDCHTGD SIAVNGTCLT VTDFDRYHFT 70 80 90 100 110 120 VGIAPESLRL TNLGQCKAGD PVNLERAVLS STRMGGHFVQ GHVDTVAEIV EKKQDGEAID 130 140 150 160 170 180 FTFRPRDPFV LKYIVYKGYI ALDGTSLTIT HVDDSTFSIM MISYTQSKVI MAKKNVGDLV 190 200 NVEVDQIGKY TEKLVEAHIA DWIKKTQA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Riboflavin synthase of Schizosaccharomyces pombe. Protein dynamics revealed by 19F NMR protein perturbation experiments." Fischer M., Schott A.-K., Kemter K., Feicht R., Richter G., Illarionov B., Eisenreich W., Gerhardt S., Cushman M., Steinbacher S., Huber R., Bacher A. BMC Biochem. 4:18-18(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBUNIT.
[2]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.
[3]	"Studies on the reaction mechanism of riboflavin synthase: X-ray crystal structure of a complex with 6-carboxyethyl-7-oxo-8-ribityllumazine." Gerhardt S., Schott A.-K., Kairies N., Cushman M., Illarionov B., Eisenreich W., Bacher A., Huber R., Steinbacher S., Fischer M. Structure 10:1371-1381(2002) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF505789 mRNA. Translation: AAM28201.1.
CU329672 Genomic DNA. Translation: CAB40180.1.

PIR

T40995.

RefSeq

NP_588312.1. NM_001023302.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1KZL	X-ray	2.10	A	1-208	[»]

ProteinModelPortal

Q9Y7P0.

SMR

Q9Y7P0. Positions 1-202.

ModBase

Search...

Protein-protein interaction databases

STRING

4896.SPCC1450.13c-1.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

SPCC1450.13c.1; SPCC1450.13c.1:pep; SPCC1450.13c.

GeneID

2539183.

KEGG

spo:SPCC1450.13c.

Organism-specific databases

PomBase

SPCC1450.13c.

Phylogenomic databases

eggNOG

COG0307.

HOGENOM

HOG000151757.

KO

K00793.

OMA

SRYVVEK.

OrthoDB

EOG4KSSV0.

Enzyme and pathway databases

BRENDA

2.5.1.9. 5615.

UniPathway

UPA00275; UER00405.

Family and domain databases

Gene3D

2.40.30.20. 2 hits.

InterPro

IPR023366. ATPase_F1/A1-cplx_a_su_N.
IPR001783. Lumazine-bd.
IPR026017. Lumazine-bd_dom.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]

PANTHER

PTHR21098. PTHR21098. 1 hit.

Pfam

PF00677. Lum_binding. 2 hits.
[Graphical view]

PIRSF

PIRSF000498. Riboflavin_syn_A. 1 hit.

SUPFAM

SSF63380. Riboflavin_synthase_like_b-brl. 2 hits.

TIGRFAMs

TIGR00187. ribE. 1 hit.

PROSITE

PS51177. LUMAZINE_BIND. 2 hits.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q9Y7P0.

NextBio

20800354.

Entry information

Entry name

RISA_SCHPO

Accession

Primary (citable) accession number: Q9Y7P0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 2005
Last sequence update:	November 1, 1999
Last modified:	May 1, 2013
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program