Q9Y7K2 (TOR2_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphatidylinositol 3-kinase tor2
Short name=PI3-kinase tor2
Short name=PI3K tor2
Short name=PtdIns-3-kinase tor2
EC=2.7.1.137

Gene names

Name:	tor2
ORF Names:	SPBC216.07c, SPBC646.01c

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › Schizosaccharomyces pombe

Protein attributes

Sequence length	2337 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Phosphatidylinositol 3-kinase homolog required for G1 progression By similarity.
Catalytic activity	ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 3-phosphate.
Sequence similarities	Belongs to the PI3/PI4-kinase family. Contains 1 FAT domain. Contains 1 FATC domain. Contains 14 HEAT repeats. Contains 1 PI3K/PI4K domain.

Ontologies

Keywords
Biological process	Cell cycle
Domain	Repeat
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	TOR signaling cascade Traceable author statement PubMed 18076573. Source: PomBase cell cycle arrest in response to nitrogen starvation Inferred from mutant phenotype PubMed 17179073PubMed 18076573. Source: PomBase cellular response to nutrient Non-traceable author statement. Source: PomBase negative regulation of autophagy Inferred from mutant phenotype PubMed 17121544. Source: PomBase negative regulation of cell cycle arrest in response to nitrogen starvation Inferred from mutant phenotype PubMed 17046992PubMed 17121544PubMed 17261596. Source: PomBase negative regulation of induction of conjugation with cellular fusion Inferred from mutant phenotype PubMed 17179073. Source: PomBase phosphatidylinositol-3-phosphate biosynthetic process Inferred from electronic annotation. Source: GOC
Cellular_component	TORC1 complex Inferred from direct assay PubMed 17046992PubMed 17261596. Source: PomBase Tor2-Mei2-Ste11 complex Inferred from direct assay PubMed 17046992. Source: PomBase cytosol Inferred from direct assay PubMed 16823372. Source: PomBase
Molecular_function	1-phosphatidylinositol-3-kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW drug binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
tel2	Q9P3W5	3	EBI-2014299,EBI-2014377

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 2337	2337	Phosphatidylinositol 3-kinase tor2		PRO_0000088814
Regions
	Repeat	163 – 200	38	HEAT 1
	Repeat	249 – 286	38	HEAT 2
	Repeat	290 – 326	37	HEAT 3
	Repeat	409 – 446	38	HEAT 4
	Repeat	474 – 512	39	HEAT 5
	Repeat	559 – 596	38	HEAT 6
	Repeat	642 – 679	38	HEAT 7
	Repeat	683 – 721	39	HEAT 8
	Repeat	727 – 765	39	HEAT 9
	Repeat	802 – 840	39	HEAT 10
	Repeat	844 – 880	37	HEAT 11
	Repeat	881 – 921	41	HEAT 12
	Repeat	965 – 1004	40	HEAT 13
	Repeat	1006 – 1043	38	HEAT 14
	Domain	1228 – 1784	557	FAT
	Domain	1990 – 2304	315	PI3K/PI4K
	Domain	2305 – 2337	33	FATC
Amino acid modifications
	Modified residue	2238	1	Phosphoserine Ref.3
	Modified residue	2240	1	Phosphoserine Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q9Y7K2 [UniParc]. Last modified December 6, 2002. Version 2. Checksum: 190F448DA04FD2D9	FASTA	2,337	266,379
	10 20 30 40 50 60 MKEFPGLKSR NEEIRNKAAN DLYEYVIAYS RELSGEALVQ FNNDVNKYVY TLIHSTDPLD 70 80 90 100 110 120 RLAGVTAINR LIDYEGEDTT RITRFANYLR IILPGTDQKA TVLAAKALGR LAVPGGALTS 130 140 150 160 170 180 EFVNFEVKRA LEWLQGERNE NRRYAAVLIL KELAKNTSTL IYAHIDSIFE LLWHGLRDPK 190 200 210 220 230 240 VTIRIASADA LSEFLKIVRQ RDSSIRLQWY TSILNEAQRG VAQGSSDYIH GSLLVYRQLF 250 260 270 280 290 300 LKAGMFMHER YREVSDIILQ FRDHKDLLIR KTVTELIATL AAYNPDEFVS NYLHVCMLHL 310 320 330 340 350 360 LNLLKKENVK MLAFATIGKV AVAITNSIIP YLDPICDSIK ESLKIHIRNK SASDAAIFQC 370 380 390 400 410 420 ISLLSIALGQ AFSNYAYDLF DLIFASGLSE ASYRALSDLA HNIPPLLPVI QERLLDMLSK 430 440 450 460 470 480 ILSGRPFIPP GCPPQYVARS LKSSKSASLK TGFFPNDVYI LALKVLGNFD FSGYILNEFV 490 500 510 520 530 540 KDCVVVYLEN NDPEVRKTAS ITCSQLFARD PILSQTSDHA IQVVAEVLEK LLTVGICDTV 550 560 570 580 590 600 PDIRLTVLNS LDSRFNKHLA QADKIRLLFI AINDENFAVR ESALRIIGRL NVYNPAYVMP 610 620 630 640 650 660 YLRKIMLKTL TILDYSTIIR TKEENAKLLC LLIAAAPRLI ESHVEPILQI LLPKAKDSSS 670 680 690 700 710 720 IVAASIVNSL GEICQISGEV IVPFIKDLMP LIIEALQDQS SPIRRAAALK ALGNLSSSTG 730 740 750 760 770 780 YVIDPYIEFP SLLDILIGIT KTEQDITIRR ETIKLIGTLG ALDPNRHRVL EKGTEKVVPE 790 800 810 820 830 840 QKNIPPDISL LMSGIGPSSD EYYPTVVITA LMSILKDPSL TIHHTAVIQA VMYIFKTMGL 850 860 870 880 890 900 RCAPFLSQII PEFIAVMRTC PTNILEFYFQ QLSILVLIVR QHIRSFLPDL FKLIKDFWNP 910 920 930 940 950 960 HSNLQFTILS LIESLARAMQ GEFKPYLPSL LVMMLQIFDS DVSVDSVSTK KVLHAFIVFG 970 980 990 1000 1010 1020 DTLADYFHML LDPILRLYER NDVSIGIKES IMITIGRLSM VINLSEYASR IIHPVMRMLS 1030 1040 1050 1060 1070 1080 CNNASLIRVS MDTVCALIYQ LNVDFAIFIP MIDKCLKMNG VTHETYSILV EQFLQEQPLP 1090 1100 1110 1120 1130 1140 IKLNPYEKYD KPKLDVVASA ADITSKKLPV NQEILRNAWE ASQRSTKDDW QEWIRRLGVA 1150 1160 1170 1180 1190 1200 LLRESPSHAL RACAALAAAY QPLARDLFNA SFVSCWSELY DHFQEELVKS IEIALTSPHI 1210 1220 1230 1240 1250 1260 SPEIIQILLN LAEFMEHDDK PLPIDIRTLG AYAAKCHAFA KALHYKELEF IEEELVTKPS 1270 1280 1290 1300 1310 1320 VDTIEALISI NNQLQQPDAA IGILKHAQQH DKMNLKETWY EKLQRWEDAL SAYEKREAAG 1330 1340 1350 1360 1370 1380 AGNFEITMGK LRCLHALGEW DRLSQLAQEN WIHAGHDARR YIAPLSVAAA WGLGQWEQMD 1390 1400 1410 1420 1430 1440 EYISVMKSES PDKAFFNAIV ALHRSQFEEA ASYITRARDL LDTELTALVG ESYNRAYSVA 1450 1460 1470 1480 1490 1500 VRVQMLSELE EIITYKKAED KPEVREMIKK TWVRRLKGCQ RNVDVWQRML RIRSLVISPR 1510 1520 1530 1540 1550 1560 DNMEMWIKFA NLCRKSGRIS LAKKSLNLLL EDDENLDNSL VLKKTHPSIV YANLKFLWAV 1570 1580 1590 1600 1610 1620 DDKRKALNSM QEFTSQLISD INVDPALFVQ STSVNTQKSQ EEIQYYFHLL ARCYHKQGQW 1630 1640 1650 1660 1670 1680 QQEIENNWSE GSFDGVLQSY MYATQFDSKW YKAWHSWALA NFEAVKFLEQ SEEQIPSAAY 1690 1700 1710 1720 1730 1740 EQYIIPAVKG FFKSIALSKG NLQDTLRLLN LWFKFGNNSN VINTLNVGIS TVNIDIWLDV 1750 1760 1770 1780 1790 1800 IPQLIARIHA SSLSVRKSVH QLLSDVGRAH PQALVYPLTV AAKSQSSARQ NAALAIMDSL 1810 1820 1830 1840 1850 1860 KTHSPRLVEQ ARLVSHELIR AAILWHEQWH EGLEEASRLY FGDHNIEGMF AVLRPLHEML 1870 1880 1890 1900 1910 1920 ERGPETLREI SFQQAFGRDL VEARDCCIRF EQTGDISDLN QAWDLYYQVF KKIRKQLPQL 1930 1940 1950 1960 1970 1980 TTLDLQYVSP KLLHVHDLEL AVPGTYVSGK PVIRIVKFYP TFNVITSKQR PRRLSIKGSD 1990 2000 2010 2020 2030 2040 GKDYQYVLKG HEDIRQDERV MQLFGLCNNL LLADPETFKR LLSIQRYPVI PLSPDSGLLG 2050 2060 2070 2080 2090 2100 WVLDSDTLHV LIRDYRESRK ILLNIEHRLI IQMAPDYDRL TLLQKVEVFE YALLSTTGQD 2110 2120 2130 2140 2150 2160 LYRVLWLKSR SSEAWLNRRT NYSRTLAVMS MVGYILGLGD RHPSNLMLDR YTGNIIHIDF 2170 2180 2190 2200 2210 2220 GDCFEVAMHR EKFPEKIPFR LTRMLVNAME VSGIEGTFRI TCEHVMRVLR TNKESVMAVL 2230 2240 2250 2260 2270 2280 EAFVYDPLIN WRLAPAYSPS IDEKQSNEPN TLLGETIDGL HRKRLNEEGI TLEERQKPEI 2290 2300 2310 2320 2330 LNQRAITVLN RVSNKLTGRD FKPQQQLDVP SQVEKLILQA TSIENLCLCY IGWCSFW

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.
[2]	"The fission yeast TOR homolog, tor1+, is required for the response to starvation and other stresses via a conserved serine." Weisman R., Choder M. J. Biol. Chem. 276:7027-7032(2001) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION.
[3]	"Phosphoproteome analysis of fission yeast." Wilson-Grady J.T., Villen J., Gygi S.P. J. Proteome Res. 7:1088-1097(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-2238 AND SER-2240, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU329671 Genomic DNA. Translation: CAB40167.1.
PIR	T40577. T39913.
RefSeq	NP_595359.2. NM_001021266.3.
3D structure databases
ProteinModelPortal	Q9Y7K2.
SMR	Q9Y7K2. Positions 1817-1916, 2306-2337.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9Y7K2. 7 interactions.
STRING	4896.SPBC216.07c-1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPBC216.07c.1; SPBC216.07c.1:pep; SPBC216.07c.
GeneID	2540720.
KEGG	spo:SPBC216.07c.
Organism-specific databases
PomBase	SPBC216.07c.
Phylogenomic databases
eggNOG	COG5032.
HOGENOM	HOG000163215.
KO	K07203.
OMA	DPYKHKM.
OrthoDB	EOG4ZCXCJ.
Family and domain databases
Gene3D	1.10.1070.11. 3 hits. 1.25.10.10. 6 hits.
InterPro	IPR011989. ARM-like. IPR016024. ARM-type_fold. IPR024585. DUF3385_TOR. IPR003152. FATC. IPR021133. HEAT_type_2. IPR011009. Kinase-like_dom. IPR000403. PI3/4_kinase_cat_dom. IPR018936. PI3/4_kinase_CS. IPR003151. PIK-rel_kinase_FAT. IPR014009. PIK_FAT. IPR009076. Rapamycin-bd_dom. IPR026683. TOR/Smg1. [Graphical view]
PANTHER	PTHR11139:SF9. PTHR11139:SF9. 1 hit.
Pfam	PF11865. DUF3385. 1 hit. PF02259. FAT. 1 hit. PF02260. FATC. 1 hit. PF00454. PI3_PI4_kinase. 1 hit. PF08771. Rapamycin_bind. 1 hit. [Graphical view]
SMART	SM00146. PI3Kc. 1 hit. [Graphical view]
SUPFAM	SSF48371. ARM-type_fold. 2 hits. SSF47212. FRAP_FKBP12_bind. 1 hit. SSF56112. Kinase_like. 1 hit.
PROSITE	PS51189. FAT. 1 hit. PS51190. FATC. 1 hit. PS50077. HEAT_REPEAT. 1 hit. PS00915. PI3_4_KINASE_1. 1 hit. PS00916. PI3_4_KINASE_2. 1 hit. PS50290. PI3_4_KINASE_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20801842.

Entry information

Entry name

TOR2_SCHPO

Accession

Primary (citable) accession number: Q9Y7K2
Secondary accession number(s): O94507

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2002
Last sequence update:	December 6, 2002
Last modified:	May 1, 2013
This is version 96 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program