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Protein

Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase

Gene

alg3

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Adds the first Dol-P-Man derived mannose in an alpha-1,3 linkage to Man5GlcNAc2-PP-Dol.By similarity

Catalytic activityi

Dolichyl beta-D-mannosyl phosphate + D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->3)-D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol + dolichyl phosphate.

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase

Enzyme and pathway databases

BRENDAi2.4.1.258 5613
ReactomeiR-SPO-446193 Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein
UniPathwayiUPA00378

Protein family/group databases

CAZyiGT58 Glycosyltransferase Family 58

Names & Taxonomyi

Protein namesi
Recommended name:
Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase (EC:2.4.1.258)
Alternative name(s):
Asparagine-linked glycosylation protein 3
Dol-P-Man-dependent alpha(1-3)-mannosyltransferase
Dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichyl mannosyltransferase
Dolichyl-phosphate-mannose--glycolipid alpha-mannosyltransferase
Gene namesi
Name:alg3
ORF Names:SPAC7D4.06c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC7D4.06c
PomBaseiSPAC7D4.06c alg3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei72 – 92HelicalSequence analysisAdd BLAST21
Transmembranei107 – 127HelicalSequence analysisAdd BLAST21
Transmembranei129 – 149HelicalSequence analysisAdd BLAST21
Transmembranei186 – 206HelicalSequence analysisAdd BLAST21
Transmembranei214 – 234HelicalSequence analysisAdd BLAST21
Transmembranei261 – 281HelicalSequence analysisAdd BLAST21
Transmembranei313 – 333HelicalSequence analysisAdd BLAST21
Transmembranei349 – 369HelicalSequence analysisAdd BLAST21
Transmembranei376 – 396HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003277641 – 406Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferaseAdd BLAST406

Proteomic databases

PaxDbiQ9Y7I4
PRIDEiQ9Y7I4

Interactioni

Protein-protein interaction databases

BioGridi279218, 1 interactor
STRINGi4896.SPAC7D4.06c.1

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 58 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000237555
InParanoidiQ9Y7I4
KOiK03845
OMAiTKKVPPY
OrthoDBiEOG092C26UZ
PhylomeDBiQ9Y7I4

Family and domain databases

InterProiView protein in InterPro
IPR007873 Glycosyltransferase_ALG3
PANTHERiPTHR12646 PTHR12646, 1 hit
PfamiView protein in Pfam
PF05208 ALG3, 1 hit

Sequencei

Sequence statusi: Complete.

Q9Y7I4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSVETRNSF NPFRVLFDLG SYGWLHPSRL LLLEIPFVFA IISKVPYTEI
60 70 80 90 100
DWIAYMEQVN SFLLGERDYK SLVGCTGPLV YPGGHVFLYT LLYYLTDGGT
110 120 130 140 150
NIVRAQYIFA FVYWITTAIV GYLFKIVRAP FYIYVLLILS KRLHSIFILR
160 170 180 190 200
LFNDGFNSLF SSLFILSSCK KKWVRASILL SVACSVKMSS LLYVPAYLVL
210 220 230 240 250
LLQILGPKKT WMHIFVIIIV QILFSIPFLA YFWSYWTQAF DFGRAFDYKW
260 270 280 290 300
TVNWRFIPRS IFESTSFSTS ILFLHVALLV AFTCKHWNKL SRATPFAMVN
310 320 330 340 350
SMLTLKPLPK LQLATPNFIF TALATSNLIG ILCARSLHYQ FYAWFAWYSP
360 370 380 390 400
YLCYQASFPA PIVIGLWMLQ EYAWNVFPST KLSSLIAVCV PLITILKLYT

SDYRKP
Length:406
Mass (Da):46,992
Last modified:November 1, 1999 - v1
Checksum:i039E40A16A9359C8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA Translation: CAB16723.2
PIRiT39084
RefSeqiNP_593853.1, NM_001019282.2

Genome annotation databases

EnsemblFungiiSPAC7D4.06c.1; SPAC7D4.06c.1:pep; SPAC7D4.06c
GeneIDi2542768
KEGGispo:SPAC7D4.06c

Similar proteinsi

Entry informationi

Entry nameiALG3_SCHPO
AccessioniPrimary (citable) accession number: Q9Y7I4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: November 1, 1999
Last modified: April 25, 2018
This is version 95 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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