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Protein

Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase

Gene

alg3

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Adds the first Dol-P-Man derived mannose in an alpha-1,3 linkage to Man5GlcNAc(2)-PP-Dol.By similarity

Catalytic activityi

Dolichyl beta-D-mannosyl phosphate + D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->3)-D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol + dolichyl phosphate.

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BRENDAi2.4.1.258. 5613.
ReactomeiR-SPO-446193. Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT58. Glycosyltransferase Family 58.

Names & Taxonomyi

Protein namesi
Recommended name:
Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase (EC:2.4.1.258)
Alternative name(s):
Asparagine-linked glycosylation protein 3
Dol-P-Man-dependent alpha(1-3)-mannosyltransferase
Dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichyl mannosyltransferase
Dolichyl-phosphate-mannose--glycolipid alpha-mannosyltransferase
Gene namesi
Name:alg3
ORF Names:SPAC7D4.06c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC7D4.06c.
PomBaseiSPAC7D4.06c. alg3.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei72 – 92HelicalSequence analysisAdd BLAST21
Transmembranei107 – 127HelicalSequence analysisAdd BLAST21
Transmembranei129 – 149HelicalSequence analysisAdd BLAST21
Transmembranei186 – 206HelicalSequence analysisAdd BLAST21
Transmembranei214 – 234HelicalSequence analysisAdd BLAST21
Transmembranei261 – 281HelicalSequence analysisAdd BLAST21
Transmembranei313 – 333HelicalSequence analysisAdd BLAST21
Transmembranei349 – 369HelicalSequence analysisAdd BLAST21
Transmembranei376 – 396HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003277641 – 406Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferaseAdd BLAST406

Proteomic databases

PRIDEiQ9Y7I4.

Interactioni

Protein-protein interaction databases

BioGridi279218. 1 interactor.
MINTiMINT-4716017.

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 58 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000237555.
InParanoidiQ9Y7I4.
KOiK03845.
OMAiDWKAYMD.
OrthoDBiEOG092C26UZ.
PhylomeDBiQ9Y7I4.

Family and domain databases

InterProiIPR007873. Glycosyltransferase_ALG3.
[Graphical view]
PANTHERiPTHR12646. PTHR12646. 1 hit.
PfamiPF05208. ALG3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9Y7I4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSVETRNSF NPFRVLFDLG SYGWLHPSRL LLLEIPFVFA IISKVPYTEI
60 70 80 90 100
DWIAYMEQVN SFLLGERDYK SLVGCTGPLV YPGGHVFLYT LLYYLTDGGT
110 120 130 140 150
NIVRAQYIFA FVYWITTAIV GYLFKIVRAP FYIYVLLILS KRLHSIFILR
160 170 180 190 200
LFNDGFNSLF SSLFILSSCK KKWVRASILL SVACSVKMSS LLYVPAYLVL
210 220 230 240 250
LLQILGPKKT WMHIFVIIIV QILFSIPFLA YFWSYWTQAF DFGRAFDYKW
260 270 280 290 300
TVNWRFIPRS IFESTSFSTS ILFLHVALLV AFTCKHWNKL SRATPFAMVN
310 320 330 340 350
SMLTLKPLPK LQLATPNFIF TALATSNLIG ILCARSLHYQ FYAWFAWYSP
360 370 380 390 400
YLCYQASFPA PIVIGLWMLQ EYAWNVFPST KLSSLIAVCV PLITILKLYT

SDYRKP
Length:406
Mass (Da):46,992
Last modified:November 1, 1999 - v1
Checksum:i039E40A16A9359C8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB16723.2.
PIRiT39084.
RefSeqiNP_593853.1. NM_001019282.2.

Genome annotation databases

EnsemblFungiiSPAC7D4.06c.1; SPAC7D4.06c.1:pep; SPAC7D4.06c.
GeneIDi2542768.
KEGGispo:SPAC7D4.06c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB16723.2.
PIRiT39084.
RefSeqiNP_593853.1. NM_001019282.2.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi279218. 1 interactor.
MINTiMINT-4716017.

Protein family/group databases

CAZyiGT58. Glycosyltransferase Family 58.

Proteomic databases

PRIDEiQ9Y7I4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC7D4.06c.1; SPAC7D4.06c.1:pep; SPAC7D4.06c.
GeneIDi2542768.
KEGGispo:SPAC7D4.06c.

Organism-specific databases

EuPathDBiFungiDB:SPAC7D4.06c.
PomBaseiSPAC7D4.06c. alg3.

Phylogenomic databases

HOGENOMiHOG000237555.
InParanoidiQ9Y7I4.
KOiK03845.
OMAiDWKAYMD.
OrthoDBiEOG092C26UZ.
PhylomeDBiQ9Y7I4.

Enzyme and pathway databases

UniPathwayiUPA00378.
BRENDAi2.4.1.258. 5613.
ReactomeiR-SPO-446193. Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein.

Miscellaneous databases

PROiQ9Y7I4.

Family and domain databases

InterProiIPR007873. Glycosyltransferase_ALG3.
[Graphical view]
PANTHERiPTHR12646. PTHR12646. 1 hit.
PfamiPF05208. ALG3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiALG3_SCHPO
AccessioniPrimary (citable) accession number: Q9Y7I4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: November 1, 1999
Last modified: November 30, 2016
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.