Reviewed, UniProtKB/Swiss-Prot Q9Y753 (FDFT_YARLI)

Names and origin

Protein names

Recommended name:
    Squalene synthetase
      Short name=SQS
      Short name=SS
    EC=2.5.1.21
Alternative name(s):
    Farnesyl-diphosphate farnesyltransferase
    FPP:FPP farnesyltransferase

Gene names

Name:	SQS1
Ordered Locus Names:	YALI0A10076g

Organism

Yarrowia lipolytica (Candida lipolytica) [Complete proteome]

Taxonomic identifier

4952 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Dipodascaceae › Yarrowia

Protein attributes

Sequence length	445 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2 farnesyl diphosphate = diphosphate + presqualene diphosphate. Presqualene diphosphate + NAD(P)H = squalene + diphosphate + NAD(P)+.
Cofactor	Magnesium.
Pathway	Terpene metabolism; lanosterol biosynthesis; lanosterol from farnesyl diphosphate: step 1/3.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the phytoene/squalene synthetase family.

Ontologies

Keywords
Biological process	Cholesterol biosynthesis Isoprene biosynthesis Lipid synthesis Steroid biosynthesis Sterol biosynthesis
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane
Ligand	Magnesium NADP
Molecular function	Oxidoreductase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	cholesterol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW isoprenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	farnesyl-diphosphate farnesyltransferase activity Inferred from electronic annotation. Source: EC heat shock protein binding Inferred from electronic annotation. Source: InterPro magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW oxidoreductase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 445	445	Squalene synthetase		PRO_0000067453
Regions
	Transmembrane	291 – 311	21	Potential
	Transmembrane	405 – 425	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9Y753-1 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: 5028E55F0A66DC7D	FASTA	445	51,145
	10 20 30 40 50 60 MGKLIELLLH PSELSAAIHY KLWRQPLHPR DLSKESTELR RCYELLDVCS RSFAAVIREL 70 80 90 100 110 120 HPEVRDAVML FYLILRALDT IEDDMTLSRD IKIPILRDFT KCMKTPGWKF TDSDPNERDR 130 140 150 160 170 180 VVLQEFPVVM TEFNKLKPKY QEVIYDITDR MGNGMADYVI DDDFNNNGVD TIAAYDLYCH 190 200 210 220 230 240 HVAGIVGEGL TRITILAGFG TDVLHENPRL QESMGLFLQK VNIIRDYRED IDVNRAFWPR 250 260 270 280 290 300 EIWHKYAEEM RDFKDPKYSK KALHCTSDLV ANALGHATDC LDYLDNVTDP STFTFCAIPQ 310 320 330 340 350 360 VMAIATLDLV YRNPDVFQKN VKLRKGTTVS LILEASNVSG VCDIFTRYAR KVYKKSDPND 370 380 390 400 410 420 PNYFRVSVLC GKIEQHAALI KRQRGPPAKT IAQLEGERKE MALSLIVCLA VIFSMSGLMA 430 440 YIAYVSGFRW SPREIFDSKM FPLRD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of the Yarrowia lipolytica squalene synthase (SQS1) gene and functional complementation of the Saccharomyces cerevisiae erg9 mutation." Merkulov S., van Assema F., Springer J., Fernandez Del Carmen A., Mooibroek H. Yeast 16:197-206(2000) [PubMed: 10649449] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Genome evolution in yeasts." Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S. , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L. Nature 430:35-44(2004) [PubMed: 15229592] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CLIB 122 / E 150.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF092497 Genomic DNA. Translation: AAD22408.1. CR382127 Genomic DNA. Translation: CAG83856.1.
RefSeq	XP_499929.1.
3D structure databases
SMR	Q9Y753. Positions 38-383.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9Y753.
Genome annotation databases
GeneID	2906604.
GenomeReviews	Gene locus YALI0A10076g in contig CR382127_GR.
KEGG	yli:YALI0A10076g.
Phylogenomic databases
eggNOG	fuNOG05680.
HOGENOM	HBG600510.
OMA	RSFWPKE.
OrthoDB	EOG9BZPJW.
PhylomeDB	Q9Y753.
Enzyme and pathway databases
BRENDA	2.5.1.21. 3602.
Family and domain databases
InterPro	IPR001623. DnaJ_N. IPR002060. Squ/phyt_synthse. IPR006449. Squal_synth. IPR019845. Squalene/phytoene_synthase_CS. IPR008949. Terpenoid_synth. [Graphical view]
Gene3D	G3DSA:1.10.600.10. Terpenoid_synth. 1 hit.
Pfam	PF00494. SQS_PSY. 1 hit. [Graphical view]
SMART	SM00271. DnaJ. 1 hit. [Graphical view]
TIGRFAMs	TIGR01559. squal_synth. 1 hit.
PROSITE	PS01044. SQUALEN_PHYTOEN_SYN_1. 1 hit. PS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FDFT_YARLI

Accession

Primary (citable) accession number: Q9Y753

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 31, 2004
Last sequence update:	November 1, 1999
Last modified:	February 9, 2010
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)