Reviewed, UniProtKB/Swiss-Prot Q9Y718 (PGLR_PENDI)

Names and origin

Protein names

Recommended name:
    Polygalacturonase
      Short name=PG
    EC=3.2.1.15
Alternative name(s):
    Pectinase

Gene names

Name:

PG1

Organism

Penicillium digitatum

Taxonomic identifier

36651 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Penicillium

Protein attributes

Sequence length	367 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Random hydrolysis of (1->4)-alpha-D-galactosiduronic linkages in pectate and other galacturonans.
Subcellular location	Secreted Probable.
Sequence similarities	Belongs to the glycosyl hydrolase 28 family. Contains 5 PbH1 repeats.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Secreted
Domain	Repeat Signal
Molecular function	Glycosidase Hydrolase
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	polygalacturonase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Potential
	Chain	19 – 367	349	Polygalacturonase		PRO_0000024787
Sites
	Active site	206	1	Proton donor By similarity
	Active site	228	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9Y718-1 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: 200223ADDD907A4D	FASTA	367	37,751
	10 20 30 40 50 60 MRTSFVTMLA LGAAAVSAAP AAPVTDLVER GSSCTFTTAE AAKAGKGSCS TIVLDNIKVP 70 80 90 100 110 120 AGETLDLTKL KSGTQVVFKG ETSFGYKEWT GPLVSFSGSN IHVSGAAGHV INGGGPSWWD 130 140 150 160 170 180 GKGTNGGKKK PKFFYAHHLD DSTISGLNVK NTPVQGFSIL ADRLTLDHIT IDNSEGDAKG 190 200 210 220 230 240 GHNTDAFDVG SSTFITISNA NIKNQDDCLA INSGSNIKFV GGTCSGGHGI SIGSVGLRDN 250 260 270 280 290 300 NIVKDVTISD STVINSDNGV RVKTIYQATG AVSGVTFSNI KLSNIAKYGI VIEQDYENGS 310 320 330 340 350 360 PTGKPTNGVP ISELTIENVT GTLKSSATEV YILCGNGSCK NWKWAGNSLS GGKKSGKCGN VPAGASC

References

[1]	"Polygalacturonase encoding gene of Penicillium digitatum." Arimoto Y., Arie T., Yamaguchi I. Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: JCM 9863.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB015286 Genomic DNA. Translation: BAA77297.1.
3D structure databases
SMR	Q9Y718. Positions 32-365.
ModBase	Search...
Protein family/group databases
CAZy	GH28. Glycoside Hydrolase Family 28.
Enzyme and pathway databases
BRENDA	3.2.1.15. 292324.
Family and domain databases
InterPro	IPR000743. Glyco_hydro_28. IPR006626. PbH1. IPR012334. Pectin_lyas_fold. IPR011050. Pectin_lyase_fold/virulence. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
Pfam	PF00295. Glyco_hydro_28. 1 hit. [Graphical view]
SMART	SM00710. PbH1. 5 hits. [Graphical view]
PROSITE	PS00502. POLYGALACTURONASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PGLR_PENDI

Accession

Primary (citable) accession number: Q9Y718

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1999
Last modified:	January 19, 2010
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)