Reviewed, UniProtKB/Swiss-Prot Q9Y705 (ALP4_SCHPO)

Names and origin

Protein names

Recommended name:
    Spindle pole body component alp4
Alternative name(s):
    Altered polarity protein 4

Gene names

Name:	alp4
ORF Names:	SPBC365.15

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	784 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Component of the gamma tubule complex that is required for the regulation of both interphase microtubules and mitotic bipolar spindles. Required for correct septation. Ref.3
Subunit structure	Interacts with mcp6. Ref.4
Subcellular location	Cytoplasm. Cytoplasm › cytoskeleton › spindle pole body. Cytoplasm › cytoskeleton › centrosome. Note: And the microtubule organizing center (MTOC). Ref.4 Ref.5
Sequence similarities	Belongs to the GCP family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis Septation
Cellular component	Cytoplasm Cytoskeleton Microtubule Nucleus
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	G2/M transition of mitotic cell cycle Inferred from mutant phenotype. Source: GeneDB_SPombe M/G1 transition of mitotic cell cycle Ref.1 Inferred from mutant phenotype. Source: GeneDB_SPombe barrier septum formation Inferred from electronic annotation. Source: UniProtKB-KW cytoplasmic microtubule organization Ref.1 Inferred from mutant phenotype. Source: GeneDB_SPombe establishment or maintenance of cell polarity Inferred from mutant phenotype. Source: GeneDB_SPombe gamma-tubulin complex localization Inferred from mutant phenotype. Source: GeneDB_SPombe microtubule anchoring at spindle pole body Inferred from mutant phenotype. Source: GeneDB_SPombe microtubule nucleation Non-traceable author statement. Source: GeneDB_SPombe mitotic cell cycle spindle assembly checkpoint Ref.1 Inferred from mutant phenotype. Source: GeneDB_SPombe mitotic metaphase/anaphase transition Inferred by curator. Source: GeneDB_SPombe nuclear migration along microtubule Inferred from mutant phenotype. Source: GeneDB_SPombe spindle assembly Ref.1 Inferred from mutant phenotype. Source: GeneDB_SPombe
Cellular component	centrosome Inferred from electronic annotation. Source: UniProtKB-SubCell cytosol Ref.5 Inferred from direct assay. Source: GeneDB_SPombe equatorial microtubule organizing center Ref.1 Inferred from direct assay. Source: GeneDB_SPombe gamma-tubulin complex Ref.1 Traceable author statement. Source: GeneDB_SPombe microtubule Inferred from electronic annotation. Source: UniProtKB-KW nucleus Ref.5 Inferred from direct assay. Source: GeneDB_SPombe spindle pole body Ref.1 Inferred from direct assay. Source: GeneDB_SPombe
Molecular function	protein binding Ref.4 Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
mcp6	Q10336	1	EBI-1562228,EBI-1562149

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 784	784	Spindle pole body component alp4		PRO_0000078132

Sequences

	Sequence		Length	Mass (Da)
	Q9Y705-1 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: E529CE217FFDA2B2	FASTA	784	90,158
	10 20 30 40 50 60 MVRSISSVLA KEESENGSPI PALEEGSVYH VSLKSEGVSP FSDRIQLNYL YDGKTFSDPS 70 80 90 100 110 120 NLNIHQQEAC LINELLNAFM GMEGVFVHLQ DMKASSEFET IIMPPSFYIL PGFDLGIKDI 130 140 150 160 170 180 ASEMLEMGSH YLSITAFIES RSHFEYGFVN HALCAALRKF VMDYVVLIMQ CENQSRIDPN 190 200 210 220 230 240 FSLQTLRLYT LPTSRSLRQV YLILRDLLLS MEKNASSSDD LGLSNIDDLL EQLNEGNDIS 250 260 270 280 290 300 HVVNATRSKK KVCKGGQVIS FLTESLTKYA GDPVARKILT YLLREASRPY TKMLNEWIHL 310 320 330 340 350 360 GLVNDPYDEF MIKIHKGITS MQLDEDYTDE YWEKRYVIRE DQVPPQLLDL QNKVLFAGKY 370 380 390 400 410 420 LNVVLECRKG VNNLASLNAK DDTQNQLLWP STFDDDNFTL NIMNAYVYAN ESLLQLLQSS 430 440 450 460 470 480 QSLYAHLYSL KHYFFLDQSD FFTTFLDNAQ HELRKPAKYI SITKLQSQLD LALRQPGTIT 490 500 510 520 530 540 ATDPHKEYVT VEVNQTSLID WLMHIVSISG LEEGTSSQGN EVWNESITKQ ADVGNETRNF 550 560 570 580 590 600 ESEHNRSTQG TSKVGSDKDI NGFETMQLCY KVPFPLSLIL SRKAIIRYQL LFRYFLLLRH 610 620 630 640 650 660 VEMQLENSWV QHSKNSAWRL NSSNAKIEQW KRNSWLLRTR MLSFVQKIIY YTTSEVIETH 670 680 690 700 710 720 WGKFMGELEN ARTVDNLMQE HIDFLDTCLK ECMLTNSRLL KVQSKLLNTC AMFASYTSTF 730 740 750 760 770 780 TRSLYLLENS EESFDEGRMD KMEEILRRYE DSFSRHLKSL VNACNYFAST ETAALLSLVM KLTG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The fission yeast gamma-tubulin complex is required in G(1) phase and is a component of the spindle assembly checkpoint." Vardy L., Toda T. EMBO J. 19:6098-6111(2000) [PubMed: 11080156] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION. Strain: ATCC 38366 / 972.
[2]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.
[3]	"The gamma-tubulin complex protein Alp4 provides a link between the metaphase checkpoint and cytokinesis in fission yeast." Vardy L., Fujita A., Toda T. Genes Cells 7:365-373(2002) [PubMed: 11952833] [Abstract] Cited for: FUNCTION.
[4]	"Hrs1p/Mcp6p on the meiotic SPB organizes astral microtubule arrays for oscillatory nuclear movement." Tanaka K., Kohda T., Yamashita A., Nonaka N., Yamamoto M. Curr. Biol. 15:1479-1486(2005) [PubMed: 16111942] [Abstract] Cited for: INTERACTION WITH MCP6, SUBCELLULAR LOCATION.
[5]	"ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe." Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M. Nat. Biotechnol. 24:841-847(2006) [PubMed: 16823372] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CU329671 Genomic DNA. Translation: CAB44767.1. AB026664 Genomic DNA. Translation: BAA77269.1.
PIR	T43510.
RefSeq	NP_596044.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9Y705. 2 interactions.
STRING	Q9Y705.
Genome annotation databases
GeneID	2540392.
GenomeReviews	Gene locus alp4 in contig CU329671_GR.
KEGG	spo:SPBC365.15.
NMPDR	fig|4896.1.peg.1910.
Organism-specific databases
GeneDB_Spombe	SPBC365.15.
Phylogenomic databases
OMA	YTDEYWE
OrthoDB	EOG9X6DS7
Gene expression databases
ArrayExpress	Q9Y705.
Family and domain databases
InterPro	IPR007259. Spc97_Spc98. [Graphical view]
PANTHER	PTHR19302. Spc97_Spc98. 1 hit.
Pfam	PF04130. Spc97_Spc98. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALP4_SCHPO

Accession

Primary (citable) accession number: Q9Y705

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 29, 2001
Last sequence update:	November 1, 1999
Last modified:	November 24, 2009
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)