Reviewed, UniProtKB/Swiss-Prot Q9Y703 (TBCA_SCHPO)

Names and origin

Protein names

Recommended name:
    Tubulin-specific chaperone A
Alternative name(s):
    Tubulin-folding cofactor A
      Short name=CFA
    Altered polarity protein 31

Gene names

Name:	alp31
ORF Names:	SPAC8E11.07c

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	119 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Required for the maintenance of microtubule structures and cell polarity. Beta-tubulin-folding protein; may have a regulatory role in the tubulin-folding pathway. Ref.1
Subunit structure	Supercomplex made of cofactors A to E. Cofactors A and D function by capturing and stabilizing tubulin in a quasi-native conformation. Cofactor E binds to the cofactor D-tubulin complex; interaction with cofactor C then causes the release of tubulin polypeptides that are committed to the native state By similarity.
Subcellular location	Cytoplasm › cytoskeleton.
Sequence similarities	Belongs to the TBCA family.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton Microtubule
Molecular function	Chaperone
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	establishment or maintenance of microtubule cytoskeleton polarity Ref.1 Inferred from mutant phenotype. Source: GeneDB_SPombe post-chaperonin tubulin folding pathway Ref.1 Inferred from mutant phenotype. Source: GeneDB_SPombe tubulin complex assembly Inferred from electronic annotation. Source: InterPro
Cellular component	cytosol Inferred from direct assay. Source: GeneDB_SPombe microtubule Inferred from electronic annotation. Source: UniProtKB-KW nucleus Inferred from direct assay. Source: GeneDB_SPombe
Molecular function	unfolded protein binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 119	119	Tubulin-specific chaperone A		PRO_0000080044

Sequences

	Sequence		Length	Mass (Da)
	Q9Y703-1 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: C1068D30FD34CAC0	FASTA	119	13,531
	10 20 30 40 50 60 MSNTVRSLVI KTNVVKRIIK DVELAHIDIN EAEKRVQSKI DNGEDSAEIE HQKFVLKKHL 70 80 90 100 110 EALPDALVRL RNATNDLESI SSDSAYEGTP ELEQANEYLE KAKEVIEKEQ TNFPTNGYH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The cofactor-dependent pathways for alpha- and beta-tubulins in microtubule biogenesis are functionally different in fission yeast." Radcliffe P.A., Garcia M.A., Toda T. Genetics 156:93-103(2000) [PubMed: 10978278] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION. Strain: ATCC 38366 / 972.
[2]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB029481 Genomic DNA. Translation: BAA82293.1. CU329670 Genomic DNA. Translation: CAB40194.1.
PIR	T43514.
RefSeq	NP_594162.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q9Y703.
Genome annotation databases
GeneID	2543341.
GenomeReviews	Gene locus alp31 in contig CU329670_GR.
KEGG	spo:SPAC8E11.07c.
NMPDR	fig|4896.1.peg.4132.
Organism-specific databases
GeneDB_Spombe	SPAC8E11.07c.
Phylogenomic databases
OrthoDB	EOG912NNH
Gene expression databases
ArrayExpress	Q9Y703.
Family and domain databases
InterPro	IPR004226. CofA_tubulin_bd. [Graphical view]
PANTHER	PTHR21500. CofA_tubulin_bd. 1 hit.
Pfam	PF02970. TBCA. 1 hit. [Graphical view]
ProDom	PD010430. CofA_tubulin_bd. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

TBCA_SCHPO

Accession

Primary (citable) accession number: Q9Y703

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1999
Last modified:	November 24, 2009
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)