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Protein

Calpain-like protease palB/RIM13

Gene

palB

Organism
Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Required for the proteolytic cleavage of the transcription factor pacC in response to alkaline ambient pH. Probably is the signaling protease that mediates the first proteolytic cleavage within the signaling protease box of pacC (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei199 – 1991PROSITE-ProRule annotation
Active sitei364 – 3641PROSITE-ProRule annotation
Active sitei384 – 3841PROSITE-ProRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Protein family/group databases

MEROPSiC02.008.

Names & Taxonomyi

Protein namesi
Recommended name:
Calpain-like protease palB/RIM13 (EC:3.4.22.-)
Alternative name(s):
Cysteine protease palB
Gene namesi
Name:palB
Synonyms:palBory
ORF Names:AO090005000756
OrganismiAspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold)
Taxonomic identifieri510516 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000006564 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiFungiDB:AO090005000756.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 854854Calpain-like protease palB/RIM13PRO_0000207737Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ9Y6Z8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 8382MITAdd
BLAST
Domaini118 – 437320Calpain catalyticPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 calpain catalytic domain.PROSITE-ProRule annotation
Contains 1 MIT domain.Curated

Phylogenomic databases

HOGENOMiHOG000212111.
OrthoDBiEOG74R21M.

Family and domain databases

Gene3Di1.20.58.280. 1 hit.
InterProiIPR022684. Calpain_cysteine_protease.
IPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR007330. MIT.
IPR001300. Peptidase_C2_calpain_cat.
[Graphical view]
PfamiPF01067. Calpain_III. 1 hit.
PF00648. Peptidase_C2. 1 hit.
[Graphical view]
PRINTSiPR00704. CALPAIN.
SMARTiSM00720. calpain_III. 1 hit.
SM00230. CysPc. 1 hit.
SM00745. MIT. 1 hit.
[Graphical view]
SUPFAMiSSF116846. SSF116846. 1 hit.
SSF49758. SSF49758. 2 hits.
PROSITEiPS50203. CALPAIN_CAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9Y6Z8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRPNASAQK SFITQALKAE RDVSSATSQR QALEAAIDAA EHYMKALNLA
60 70 80 90 100
SVQKDKHALD AKCKEWLTRA EKIKESKDWQ AAARFHDKTV PEPRLPVSTR
110 120 130 140 150
KLTTREEIIL LEGAKLNGFI FPPWSTSPGS DEFKREDGES PFTDKPDLHL
160 170 180 190 200
SYPQRKVFDG WKRPSELLAK DTEDVYTKVV PVMSVPGKTD LVQDMLTDCS
210 220 230 240 250
VVASLCATTS MLERGQCTHF LPMIYPSRGS SQPSPSGKYI FRFYFNGCFR
260 270 280 290 300
KVIIDDRLPS SKTSRSLHVI DRKNPNFLWP ALVEKAYLKL RGGYDFPGSN
310 320 330 340 350
SGTDLWVLTG WIPEQVFLHN DDVTGDQLWK RLYRSFHQGD VLLTIGTGEL
360 370 380 390 400
TEREQRELGL VSEHDYAILD MKESKGRRQL LVKNPWAGAD TAPGDNGSLS
410 420 430 440 450
ASQDLPHNPP SFEPGTFWMD CEKLLQHFEN LYLNWNPEIF KYREDVHFTW
460 470 480 490 500
DLNNGRGVAG CFVNNPQFAV STENGGIVWL LLGKHFRTTG QPERPLDEYQ
510 520 530 540 550
ANEESAFISI YVFNADGKRV SLSDGALHRG PYVDSPNTLM RLEMPPRTTY
560 570 580 590 600
TVVVSEQSLP SLNQNFTLSA FSTCPVRMAK AQDKYMCVRK IQGSWTPSTA
610 620 630 640 650
GGNAESSRYP LNPQFRLEIE NDTDVSLLLE CPNTELATHV KLFWSNGNRV
660 670 680 690 700
SRVRSRDIIA DSGDYRRGGS LVEKKALEPG SYTIVCSTFA PDQLGRFTLW
710 720 730 740 750
VSSLVPCKTS PLPPEAAGRR TVISDIGVLP PGRDRMLASL QVPRLTRIKL
760 770 780 790 800
ITRSRQSIIG SHPVGPSPVL MTVELGQGPY KQILATSEDG THSDAVSGVR
810 820 830 840 850
VEDFDLQPGL EESGGIWIVI ERIGGPGGQV EDHFEVEALA EERVEIGEWI

LEDA
Length:854
Mass (Da):95,470
Last modified:November 1, 1999 - v1
Checksum:i48055DAD2E814567
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti3 – 1715RPNAS…ITQAL → SILHAALVKILPGLP in BAE55782 (PubMed:16372010).CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB020321 Genomic DNA. Translation: BAA77364.1.
AF133087 Genomic DNA. Translation: AAD28472.1.
AP007151 Genomic DNA. Translation: BAE55782.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB020321 Genomic DNA. Translation: BAA77364.1.
AF133087 Genomic DNA. Translation: AAD28472.1.
AP007151 Genomic DNA. Translation: BAE55782.1.

3D structure databases

ProteinModelPortaliQ9Y6Z8.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiC02.008.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

EuPathDBiFungiDB:AO090005000756.

Phylogenomic databases

HOGENOMiHOG000212111.
OrthoDBiEOG74R21M.

Family and domain databases

Gene3Di1.20.58.280. 1 hit.
InterProiIPR022684. Calpain_cysteine_protease.
IPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR007330. MIT.
IPR001300. Peptidase_C2_calpain_cat.
[Graphical view]
PfamiPF01067. Calpain_III. 1 hit.
PF00648. Peptidase_C2. 1 hit.
[Graphical view]
PRINTSiPR00704. CALPAIN.
SMARTiSM00720. calpain_III. 1 hit.
SM00230. CysPc. 1 hit.
SM00745. MIT. 1 hit.
[Graphical view]
SUPFAMiSSF116846. SSF116846. 1 hit.
SSF49758. SSF49758. 2 hits.
PROSITEiPS50203. CALPAIN_CAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Aspergillus oryzae palBory encodes a calpain-like protease: homology to Emericella nidulans PalB and conservation of functional regions."
    Futai E., Sorimachi H., Jeong S.-Y., Kitamoto K., Ishiura S., Suzuki K.
    J. Biosci. Bioeng. 88:438-440(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Using DNA-tagged mutagenesis to improve heterologous protein production in Aspergillus oryzae."
    Yaver D.S., Lamsa M., Munds R., Brown S.H., Otani S., Franssen L., Johnstone J.A., Brody H.
    Fungal Genet. Biol. 29:28-37(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Genome sequencing and analysis of Aspergillus oryzae."
    Machida M., Asai K., Sano M., Tanaka T., Kumagai T., Terai G., Kusumoto K., Arima T., Akita O., Kashiwagi Y., Abe K., Gomi K., Horiuchi H., Kitamoto K., Kobayashi T., Takeuchi M., Denning D.W., Galagan J.E.
    , Nierman W.C., Yu J., Archer D.B., Bennett J.W., Bhatnagar D., Cleveland T.E., Fedorova N.D., Gotoh O., Horikawa H., Hosoyama A., Ichinomiya M., Igarashi R., Iwashita K., Juvvadi P.R., Kato M., Kato Y., Kin T., Kokubun A., Maeda H., Maeyama N., Maruyama J., Nagasaki H., Nakajima T., Oda K., Okada K., Paulsen I., Sakamoto K., Sawano T., Takahashi M., Takase K., Terabayashi Y., Wortman J.R., Yamada O., Yamagata Y., Anazawa H., Hata Y., Koide Y., Komori T., Koyama Y., Minetoki T., Suharnan S., Tanaka A., Isono K., Kuhara S., Ogasawara N., Kikuchi H.
    Nature 438:1157-1161(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 42149 / RIB 40.

Entry informationi

Entry nameiPALB_ASPOR
AccessioniPrimary (citable) accession number: Q9Y6Z8
Secondary accession number(s): Q2URN3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: November 1, 1999
Last modified: April 13, 2016
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.