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Protein

Lymphocyte antigen 96

Gene

LY96

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds bacterial lipopolysaccharide (LPS) (PubMed:17803912, PubMed:17569869). Cooperates with TLR4 in the innate immune response to bacterial lipopolysaccharide (LPS), and with TLR2 in the response to cell wall components from Gram-positive and Gram-negative bacteria (PubMed:11160242, PubMed:11593030). Enhances TLR4-dependent activation of NF-kappa-B (PubMed:10359581). Cells expressing both LY96 and TLR4, but not TLR4 alone, respond to LPS (PubMed:10359581).5 Publications

GO - Molecular functioni

  • coreceptor activity Source: ProtInc
  • lipopolysaccharide receptor activity Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Immunity, Inflammatory response, Innate immunity

Enzyme and pathway databases

ReactomeiR-HSA-140534. Ligand-dependent caspase activation.
R-HSA-166016. Toll Like Receptor 4 (TLR4) Cascade.
R-HSA-166058. MyD88:Mal cascade initiated on plasma membrane.
R-HSA-166166. MyD88-independent TLR3/TLR4 cascade.
R-HSA-2562578. TRIF-mediated programmed cell death.
R-HSA-5602498. MyD88 deficiency (TLR2/4).
R-HSA-5603041. IRAK4 deficiency (TLR2/4).
R-HSA-936964. Activation of IRF3/IRF7 mediated by TBK1/IKK epsilon.
R-HSA-937041. IKK complex recruitment mediated by RIP1.
R-HSA-937072. TRAF6 mediated induction of TAK1 complex.

Names & Taxonomyi

Protein namesi
Recommended name:
Lymphocyte antigen 96
Short name:
Ly-96
Alternative name(s):
ESOP-11 Publication
Protein MD-21 Publication
Gene namesi
Name:LY96
Synonyms:ESOP1, MD2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 8

Organism-specific databases

HGNCiHGNC:17156. LY96.

Subcellular locationi

GO - Cellular componenti

  • endosome membrane Source: Reactome
  • extracellular space Source: UniProtKB-SubCell
  • intrinsic component of plasma membrane Source: UniProtKB
  • lipopolysaccharide receptor complex Source: UniProtKB
  • plasma membrane Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi95 – 951C → Y: Abolishes LPS-response. 1 Publication

Organism-specific databases

PharmGKBiPA134924906.

Chemistry

ChEMBLiCHEMBL3038513.
GuidetoPHARMACOLOGYi2890.

Polymorphism and mutation databases

BioMutaiLY96.
DMDMi296434574.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818Sequence analysisAdd
BLAST
Chaini19 – 160142Lymphocyte antigen 96PRO_0000018619Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi25 ↔ 51Combined sources2 Publications
Glycosylationi26 – 261N-linked (GlcNAc...)Combined sources1 Publication
Disulfide bondi37 ↔ 148Combined sources2 Publications
Disulfide bondi95 ↔ 105Combined sources2 Publications
Glycosylationi114 – 1141N-linked (GlcNAc...)Combined sources1 Publication

Post-translational modificationi

N-glycosylated; high-mannose.2 Publications

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ9Y6Y9.
PaxDbiQ9Y6Y9.
PeptideAtlasiQ9Y6Y9.
PRIDEiQ9Y6Y9.

PTM databases

iPTMnetiQ9Y6Y9.
PhosphoSiteiQ9Y6Y9.

Expressioni

Gene expression databases

BgeeiENSG00000154589.
CleanExiHS_LY96.
GenevisibleiQ9Y6Y9. HS.

Organism-specific databases

HPAiHPA053060.

Interactioni

Subunit structurei

Heterogeneous homopolymer formed from homodimers; disulfide-linked (PubMed:11593030, PubMed:12642668). Belongs to the lipopolysaccharide (LPS) receptor, a multi-protein complex containing at least CD14, LY96 and TLR4 (PubMed:11274165). Binds to the extracellular domains of TLR2 and TLR4 (PubMed:10359581, PubMed:11593030, PubMed:17803912). Ligand binding induces interaction with TLR4 and oligomerization of the complex.5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
TLR4O002065EBI-1539247,EBI-528701

Protein-protein interaction databases

BioGridi117170. 4 interactions.
DIPiDIP-38571N.
IntActiQ9Y6Y9. 4 interactions.
STRINGi9606.ENSP00000284818.

Structurei

Secondary structure

1
160
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi22 – 265Combined sources
Beta strandi28 – 369Combined sources
Beta strandi38 – 403Combined sources
Beta strandi45 – 506Combined sources
Beta strandi57 – 659Combined sources
Beta strandi75 – 828Combined sources
Beta strandi90 – 934Combined sources
Beta strandi97 – 1015Combined sources
Helixi103 – 1064Combined sources
Beta strandi113 – 1219Combined sources
Beta strandi129 – 13911Combined sources
Turni140 – 1434Combined sources
Beta strandi144 – 15512Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1T2Zmodel-A19-160[»]
2E56X-ray2.00A17-160[»]
2E59X-ray2.21A17-160[»]
2Z65X-ray2.70C/D19-158[»]
3FXIX-ray3.10C/D19-160[»]
3ULAX-ray3.60B/D19-158[»]
4G8AX-ray2.40C/D17-160[»]
ProteinModelPortaliQ9Y6Y9.
SMRiQ9Y6Y9. Positions 17-160.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9Y6Y9.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni119 – 1235Interaction with lipopolysaccharideCombined sources2 Publications

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410J4Q5. Eukaryota.
ENOG411154R. LUCA.
GeneTreeiENSGT00390000000742.
HOGENOMiHOG000001152.
HOVERGENiHBG032514.
InParanoidiQ9Y6Y9.
KOiK05400.
OMAiIFTEAQK.
OrthoDBiEOG091G0T85.
PhylomeDBiQ9Y6Y9.
TreeFamiTF335876.

Family and domain databases

InterProiIPR014756. Ig_E-set.
IPR003172. ML_dom.
[Graphical view]
PfamiPF02221. E1_DerP2_DerF2. 1 hit.
[Graphical view]
SMARTiSM00737. ML. 1 hit.
[Graphical view]
SUPFAMiSSF81296. SSF81296. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9Y6Y9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLPFLFFSTL FSSIFTEAQK QYWVCNSSDA SISYTYCDKM QYPISINVNP
60 70 80 90 100
CIELKRSKGL LHIFYIPRRD LKQLYFNLYI TVNTMNLPKR KEVICRGSDD
110 120 130 140 150
DYSFCRALKG ETVNTTISFS FKGIKFSKGK YKCVVEAISG SPEEMLFCLE
160
FVILHQPNSN
Length:160
Mass (Da):18,546
Last modified:May 18, 2010 - v2
Checksum:i0F92AFF583637C6B
GO
Isoform 2 (identifier: Q9Y6Y9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     38-68: DKMQYPISINVNPCIELKRSKGLLHIFYIPR → G

Note: No experimental confirmation available. Gene prediction based on EST data.
Show »
Length:130
Mass (Da):14,933
Checksum:i7D92D168412B21C7
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti56 – 561R → G.5 Publications
Corresponds to variant rs6472812 [ dbSNP | Ensembl ].
VAR_050030
Natural varianti157 – 1571P → S.
Corresponds to variant rs11466004 [ dbSNP | Ensembl ].
VAR_024532

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei38 – 6831DKMQY…FYIPR → G in isoform 2. CuratedVSP_055045Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB018549 mRNA. Translation: BAA78717.1.
AF168121 mRNA. Translation: AAF89635.1.
AB446498 mRNA. Translation: BAG55275.1.
AC022868 Genomic DNA. No translation available.
AC087672 Genomic DNA. No translation available.
BC020690 mRNA. Translation: AAH20690.1.
CCDSiCCDS56540.1. [Q9Y6Y9-2]
CCDS6216.1. [Q9Y6Y9-1]
RefSeqiNP_001182726.1. NM_001195797.1. [Q9Y6Y9-2]
NP_056179.3. NM_015364.4.
UniGeneiHs.726603.

Genome annotation databases

EnsembliENST00000284818; ENSP00000284818; ENSG00000154589. [Q9Y6Y9-1]
ENST00000518893; ENSP00000430533; ENSG00000154589. [Q9Y6Y9-2]
GeneIDi23643.
KEGGihsa:23643.
UCSCiuc003yad.4. human. [Q9Y6Y9-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB018549 mRNA. Translation: BAA78717.1.
AF168121 mRNA. Translation: AAF89635.1.
AB446498 mRNA. Translation: BAG55275.1.
AC022868 Genomic DNA. No translation available.
AC087672 Genomic DNA. No translation available.
BC020690 mRNA. Translation: AAH20690.1.
CCDSiCCDS56540.1. [Q9Y6Y9-2]
CCDS6216.1. [Q9Y6Y9-1]
RefSeqiNP_001182726.1. NM_001195797.1. [Q9Y6Y9-2]
NP_056179.3. NM_015364.4.
UniGeneiHs.726603.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1T2Zmodel-A19-160[»]
2E56X-ray2.00A17-160[»]
2E59X-ray2.21A17-160[»]
2Z65X-ray2.70C/D19-158[»]
3FXIX-ray3.10C/D19-160[»]
3ULAX-ray3.60B/D19-158[»]
4G8AX-ray2.40C/D17-160[»]
ProteinModelPortaliQ9Y6Y9.
SMRiQ9Y6Y9. Positions 17-160.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi117170. 4 interactions.
DIPiDIP-38571N.
IntActiQ9Y6Y9. 4 interactions.
STRINGi9606.ENSP00000284818.

Chemistry

ChEMBLiCHEMBL3038513.
GuidetoPHARMACOLOGYi2890.

PTM databases

iPTMnetiQ9Y6Y9.
PhosphoSiteiQ9Y6Y9.

Polymorphism and mutation databases

BioMutaiLY96.
DMDMi296434574.

Proteomic databases

MaxQBiQ9Y6Y9.
PaxDbiQ9Y6Y9.
PeptideAtlasiQ9Y6Y9.
PRIDEiQ9Y6Y9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000284818; ENSP00000284818; ENSG00000154589. [Q9Y6Y9-1]
ENST00000518893; ENSP00000430533; ENSG00000154589. [Q9Y6Y9-2]
GeneIDi23643.
KEGGihsa:23643.
UCSCiuc003yad.4. human. [Q9Y6Y9-1]

Organism-specific databases

CTDi23643.
GeneCardsiLY96.
HGNCiHGNC:17156. LY96.
HPAiHPA053060.
MIMi605243. gene.
neXtProtiNX_Q9Y6Y9.
PharmGKBiPA134924906.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410J4Q5. Eukaryota.
ENOG411154R. LUCA.
GeneTreeiENSGT00390000000742.
HOGENOMiHOG000001152.
HOVERGENiHBG032514.
InParanoidiQ9Y6Y9.
KOiK05400.
OMAiIFTEAQK.
OrthoDBiEOG091G0T85.
PhylomeDBiQ9Y6Y9.
TreeFamiTF335876.

Enzyme and pathway databases

ReactomeiR-HSA-140534. Ligand-dependent caspase activation.
R-HSA-166016. Toll Like Receptor 4 (TLR4) Cascade.
R-HSA-166058. MyD88:Mal cascade initiated on plasma membrane.
R-HSA-166166. MyD88-independent TLR3/TLR4 cascade.
R-HSA-2562578. TRIF-mediated programmed cell death.
R-HSA-5602498. MyD88 deficiency (TLR2/4).
R-HSA-5603041. IRAK4 deficiency (TLR2/4).
R-HSA-936964. Activation of IRF3/IRF7 mediated by TBK1/IKK epsilon.
R-HSA-937041. IKK complex recruitment mediated by RIP1.
R-HSA-937072. TRAF6 mediated induction of TAK1 complex.

Miscellaneous databases

EvolutionaryTraceiQ9Y6Y9.
GeneWikiiLymphocyte_antigen_96.
GenomeRNAii23643.
PROiQ9Y6Y9.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000154589.
CleanExiHS_LY96.
GenevisibleiQ9Y6Y9. HS.

Family and domain databases

InterProiIPR014756. Ig_E-set.
IPR003172. ML_dom.
[Graphical view]
PfamiPF02221. E1_DerP2_DerF2. 1 hit.
[Graphical view]
SMARTiSM00737. ML. 1 hit.
[Graphical view]
SUPFAMiSSF81296. SSF81296. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiLY96_HUMAN
AccessioniPrimary (citable) accession number: Q9Y6Y9
Secondary accession number(s): B3Y6A5, E5RJJ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: May 18, 2010
Last modified: September 7, 2016
This is version 144 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.