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Protein

SEC23-interacting protein

Gene

SEC23IP

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in the organization of endoplasmic reticulum exit sites. Specifically binds to phosphatidylinositol 3-phosphate (PI3P), phosphatidylinositol 4-phosphate (PI4P) and phosphatidylinositol 5-phosphate (PI5P).3 Publications

GO - Molecular functioni

  • metal ion binding Source: InterPro
  • poly(A) RNA binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-HSA-204005. COPII (Coat Protein 2) Mediated Vesicle Transport.

Names & Taxonomyi

Protein namesi
Recommended name:
SEC23-interacting protein
Alternative name(s):
p125
Gene namesi
Name:SEC23IP
ORF Names:MSTP053
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 10

Organism-specific databases

HGNCiHGNC:17018. SEC23IP.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: ProtInc
  • cytosol Source: Reactome
  • endoplasmic reticulum Source: UniProtKB-SubCell
  • endoplasmic reticulum-Golgi intermediate compartment Source: ProtInc
  • ER to Golgi transport vesicle Source: Reactome
  • ER to Golgi transport vesicle membrane Source: UniProtKB-SubCell
  • Golgi membrane Source: GOC
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Endoplasmic reticulum, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134964657.

Polymorphism and mutation databases

BioMutaiSEC23IP.
DMDMi55584014.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10001000SEC23-interacting proteinPRO_0000097554Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei737 – 7371PhosphoserineBy similarity
Modified residuei926 – 9261PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9Y6Y8.
MaxQBiQ9Y6Y8.
PaxDbiQ9Y6Y8.
PRIDEiQ9Y6Y8.

PTM databases

iPTMnetiQ9Y6Y8.
PhosphoSiteiQ9Y6Y8.

Expressioni

Tissue specificityi

Ubiquitously expressed with stronger levels detected in heart, liver and skeletal muscle.1 Publication

Gene expression databases

BgeeiQ9Y6Y8.
CleanExiHS_SEC23IP.
ExpressionAtlasiQ9Y6Y8. baseline and differential.
GenevisibleiQ9Y6Y8. HS.

Organism-specific databases

HPAiHPA038403.
HPA043305.

Interactioni

Subunit structurei

Interacts with SEC23A.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
HSPB11Q9Y5473EBI-1767971,EBI-747101
SSC5DA1L4H13EBI-1767971,EBI-10172867

Protein-protein interaction databases

BioGridi116365. 46 interactions.
IntActiQ9Y6Y8. 14 interactions.
MINTiMINT-4999179.
STRINGi9606.ENSP00000358071.

Structurei

3D structure databases

ProteinModelPortaliQ9Y6Y8.
SMRiQ9Y6Y8. Positions 645-709.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini644 – 70764SAMAdd
BLAST
Domaini779 – 989211DDHDPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 367367Interaction with SEC23AAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi142 – 259118Pro-richAdd
BLAST

Sequence similaritiesi

Belongs to the PA-PLA1 family.Curated
Contains 1 DDHD domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2308. Eukaryota.
ENOG410YDSX. LUCA.
GeneTreeiENSGT00530000063155.
HOGENOMiHOG000007725.
HOVERGENiHBG057256.
InParanoidiQ9Y6Y8.
OMAiEIFFALG.
OrthoDBiEOG7JDQWX.
PhylomeDBiQ9Y6Y8.
TreeFamiTF314133.

Family and domain databases

Gene3Di1.10.150.50. 1 hit.
InterProiIPR004177. DDHD_dom.
IPR001660. SAM.
IPR013761. SAM/pointed.
[Graphical view]
PfamiPF02862. DDHD. 1 hit.
PF00536. SAM_1. 1 hit.
[Graphical view]
SMARTiSM01127. DDHD. 1 hit.
SM00454. SAM. 1 hit.
[Graphical view]
SUPFAMiSSF47769. SSF47769. 1 hit.
PROSITEiPS51043. DDHD. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9Y6Y8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAERKPNGGS GGASTSSSGT NLLFSSSATE FSFNVPFIPV TQASASPASL
60 70 80 90 100
LLPGEDSTDV GEEDSFLGQT SIHTSAPQTF SYFSQVSSSS DPFGNIGQSP
110 120 130 140 150
LTTAATSVGQ SGFPKPLTAL PFTTGSQDVS NAFSPSISKA QPGAPPSSLM
160 170 180 190 200
GINSYLPSQP SSLPPSYFGN QPQGIPQPGY NPYRHTPGSS RANPYIAPPQ
210 220 230 240 250
LQQCQTPGPP AHPPPSGPPV QMYQMPPGSL PPVPSSVQSP AQQQVPARPG
260 270 280 290 300
APSVQVPSPF LLQNQYEPVQ PHWFYCKEVE YKQLWMPFSV FDSLNLEEIY
310 320 330 340 350
NSVQPDPESV VLGTDGGRYD VYLYDRIRKA AYWEEEPAEV RRCTWFYKGD
360 370 380 390 400
TDSRFIPYTE EFSEKLEAEY KKAVTTNQWH RRLEFPSGET IVMHNPKVIV
410 420 430 440 450
QFQPSSVPDE WGTTQDGQTR PRVVKRGIDD NLDEIPDGEM PQVDHLVFVV
460 470 480 490 500
HGIGPVCDLR FRSIIECVDD FRVVSLKLLR THFKKSLDDG KVSRVEFLPV
510 520 530 540 550
HWHSSLGGDA TGVDRNIKKI TLPSIGRFRH FTNETLLDIL FYNSPTYCQT
560 570 580 590 600
IVEKVGMEIN HLHALFMSRN PDFKGGVSVA GHSLGSLILF DILSNQKDLN
610 620 630 640 650
LSKCPGPLAV ANGVVKQLHF QEKQMPEEPK LTLDESYDLV VENKEVLTLQ
660 670 680 690 700
ETLEALSLSE YFSTFEKEKI DMESLLMCTV DDLKEMGIPL GPRKKIANFV
710 720 730 740 750
EHKAAKLKKA ASEKKAVAAT STKGQEQSAQ KTKDMASLPS ESNEPKRKLP
760 770 780 790 800
VGACVSSVCV NYESFEVGAG QVSVAYNSLD FEPEIFFALG SPIAMFLTIR
810 820 830 840 850
GVDRIDENYS LPTCKGFFNI YHPLDPVAYR LEPMIVPDLD LKAVLIPHHK
860 870 880 890 900
GRKRLHLELK ESLSRMGSDL KQGFISSLKS AWQTLNEFAR AHTSSTQLQE
910 920 930 940 950
ELEKVANQIK EEEEKQVVEA EKVVESPDFS KDEDYLGKVG MLNGGRRIDY
960 970 980 990 1000
VLQEKPIESF NEYLFALQSH LCYWESEDTA LLLLKEIYRT MNISPEQPQH
Length:1,000
Mass (Da):111,076
Last modified:November 1, 1999 - v1
Checksum:iDC71C274179F2CE9
GO
Isoform 2 (identifier: Q9Y6Y8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     920-924: AEKVV → GKFDI
     925-1000: Missing.

Note: No experimental confirmation available.
Show »
Length:924
Mass (Da):102,187
Checksum:i06D7CC3BC6A408D9
GO

Sequence cautioni

The sequence AAP35401.1 differs from that shown. Reason: Frameshift at position 471. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti590 – 5901F → L in AAO15299 (Ref. 2) Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti644 – 6441K → E.2 Publications
Corresponds to variant rs2475298 [ dbSNP | Ensembl ].
VAR_019806

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei920 – 9245AEKVV → GKFDI in isoform 2. 1 PublicationVSP_011831
Alternative sequencei925 – 100076Missing in isoform 2. 1 PublicationVSP_011832Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB019435 mRNA. Translation: BAA77392.1.
AF116723 mRNA. Translation: AAO15299.1.
CH471066 Genomic DNA. Translation: EAW49372.1.
CH471066 Genomic DNA. Translation: EAW49373.1.
BC063800 mRNA. Translation: AAH63800.1.
BT006755 mRNA. Translation: AAP35401.1. Frameshift.
CCDSiCCDS7618.1. [Q9Y6Y8-1]
RefSeqiNP_009121.1. NM_007190.3. [Q9Y6Y8-1]
UniGeneiHs.435004.

Genome annotation databases

EnsembliENST00000369075; ENSP00000358071; ENSG00000107651. [Q9Y6Y8-1]
GeneIDi11196.
KEGGihsa:11196.
UCSCiuc001leu.3. human. [Q9Y6Y8-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB019435 mRNA. Translation: BAA77392.1.
AF116723 mRNA. Translation: AAO15299.1.
CH471066 Genomic DNA. Translation: EAW49372.1.
CH471066 Genomic DNA. Translation: EAW49373.1.
BC063800 mRNA. Translation: AAH63800.1.
BT006755 mRNA. Translation: AAP35401.1. Frameshift.
CCDSiCCDS7618.1. [Q9Y6Y8-1]
RefSeqiNP_009121.1. NM_007190.3. [Q9Y6Y8-1]
UniGeneiHs.435004.

3D structure databases

ProteinModelPortaliQ9Y6Y8.
SMRiQ9Y6Y8. Positions 645-709.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116365. 46 interactions.
IntActiQ9Y6Y8. 14 interactions.
MINTiMINT-4999179.
STRINGi9606.ENSP00000358071.

PTM databases

iPTMnetiQ9Y6Y8.
PhosphoSiteiQ9Y6Y8.

Polymorphism and mutation databases

BioMutaiSEC23IP.
DMDMi55584014.

Proteomic databases

EPDiQ9Y6Y8.
MaxQBiQ9Y6Y8.
PaxDbiQ9Y6Y8.
PRIDEiQ9Y6Y8.

Protocols and materials databases

DNASUi11196.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000369075; ENSP00000358071; ENSG00000107651. [Q9Y6Y8-1]
GeneIDi11196.
KEGGihsa:11196.
UCSCiuc001leu.3. human. [Q9Y6Y8-1]

Organism-specific databases

CTDi11196.
GeneCardsiSEC23IP.
HGNCiHGNC:17018. SEC23IP.
HPAiHPA038403.
HPA043305.
neXtProtiNX_Q9Y6Y8.
PharmGKBiPA134964657.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2308. Eukaryota.
ENOG410YDSX. LUCA.
GeneTreeiENSGT00530000063155.
HOGENOMiHOG000007725.
HOVERGENiHBG057256.
InParanoidiQ9Y6Y8.
OMAiEIFFALG.
OrthoDBiEOG7JDQWX.
PhylomeDBiQ9Y6Y8.
TreeFamiTF314133.

Enzyme and pathway databases

ReactomeiR-HSA-204005. COPII (Coat Protein 2) Mediated Vesicle Transport.

Miscellaneous databases

ChiTaRSiSEC23IP. human.
GeneWikiiSEC23IP.
GenomeRNAii11196.
NextBioi42613.
PROiQ9Y6Y8.

Gene expression databases

BgeeiQ9Y6Y8.
CleanExiHS_SEC23IP.
ExpressionAtlasiQ9Y6Y8. baseline and differential.
GenevisibleiQ9Y6Y8. HS.

Family and domain databases

Gene3Di1.10.150.50. 1 hit.
InterProiIPR004177. DDHD_dom.
IPR001660. SAM.
IPR013761. SAM/pointed.
[Graphical view]
PfamiPF02862. DDHD. 1 hit.
PF00536. SAM_1. 1 hit.
[Graphical view]
SMARTiSM01127. DDHD. 1 hit.
SM00454. SAM. 1 hit.
[Graphical view]
SUPFAMiSSF47769. SSF47769. 1 hit.
PROSITEiPS51043. DDHD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "p125 is a novel mammalian Sec23p-interacting protein with structural similarity to phospholipid-modifying proteins."
    Tani K., Mizoguchi T., Iwamatsu A., Hatsuzawa K., Tagaya M.
    J. Biol. Chem. 274:20505-20512(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PROTEIN SEQUENCE OF 320-324; 348-361; 397-410; 477-484; 591-596; 684-693; 695-700; 825-831; 853-866; 871-877 AND 985-997, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH SEC23A.
    Tissue: Brain.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANT GLU-644.
    Tissue: Aorta.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT GLU-644.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Uterus.
  5. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
    Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
    Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-487.
  6. "A novel phospholipase A1 with sequence homology to a mammalian Sec23p-interacting protein, p125."
    Nakajima K., Sonoda H., Mizoguchi T., Aoki J., Arai H., Nagahama M., Tagaya M., Tani K.
    J. Biol. Chem. 277:11329-11335(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: LACK OF ENZYME ACTIVITY.
  7. "p125 is localized in endoplasmic reticulum exit sites and involved in their organization."
    Shimoi W., Ezawa I., Nakamoto K., Uesaki S., Gabreski G., Aridor M., Yamamoto A., Nagahama M., Tagaya M., Tani K.
    J. Biol. Chem. 280:10141-10148(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  8. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-926, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  9. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-926, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  10. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "Roles of SAM and DDHD domains in mammalian intracellular phospholipase A1 KIAA0725p."
    Inoue H., Baba T., Sato S., Ohtsuki R., Takemori A., Watanabe T., Tagaya M., Tani K.
    Biochim. Biophys. Acta 1823:930-939(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, PHOSPHOLIPID-BINDING.
  12. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-926, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiS23IP_HUMAN
AccessioniPrimary (citable) accession number: Q9Y6Y8
Secondary accession number(s): D3DRD2, Q8IXH5, Q9BUK5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 9, 2004
Last sequence update: November 1, 1999
Last modified: May 11, 2016
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Caution

Although belonging to the PA-PL1 family, does not seem to have any phospholipase activity.Curated

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 10
    Human chromosome 10: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.