ID   ZHX2_HUMAN              Reviewed;         837 AA.
AC   Q9Y6X8;
DT   01-MAR-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1999, sequence version 1.
DT   03-NOV-2009, entry version 73.
DE   RecName: Full=Zinc fingers and homeoboxes protein 2;
DE   AltName: Full=Zinc finger and homeodomain protein 2;
DE   AltName: Full=Alpha-fetoprotein regulator 1;
DE            Short=AFP regulator 1;
DE   AltName: Full=Regulator of AFP;
GN   Name=ZHX2; Synonyms=AFR1, KIAA0854, RAF;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC   Catarrhini; Hominidae; Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, HOMODIMERIZATION,
RP   HETERODIMERIZATION WITH ZHX1, SUBCELLULAR LOCATION, TISSUE
RP   SPECIFICITY, AND INTERACTION WITH NFYA.
RC   TISSUE=Testis;
RX   PubMed=12741956; DOI=10.1042/BJ20030171;
RA   Kawata H., Yamada K., Shou Z., Mizutani T., Yazawa T., Yoshino M.,
RA   Sekiguchi T., Kajitani T., Miyamoto K.;
RT   "Zinc-fingers and homeoboxes (ZHX) 2, a novel member of the ZHX
RT   family, functions as a transcriptional repressor.";
RL   Biochem. J. 373:747-757(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Brain;
RX   MEDLINE=99156230; PubMed=10048485; DOI=10.1093/dnares/5.6.355;
RA   Nagase T., Ishikawa K., Suyama M., Kikuno R., Hirosawa M.,
RA   Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.;
RT   "Prediction of the coding sequences of unidentified human genes. XII.
RT   The complete sequences of 100 new cDNA clones from brain which code
RT   for large proteins in vitro.";
RL   DNA Res. 5:355-364(1998).
RN   [3]
RP   SEQUENCE REVISION.
RA   Ohara O., Suyama M., Kikuno R., Nagase T., Ishikawa K.;
RL   Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Lymph;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA
RT   project: the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [5]
RP   STRUCTURE BY NMR OF 524-601.
RG   RIKEN structural genomics initiative (RSGI);
RT   "Solution structure of the third homeobox domain of zinc fingers and
RT   homeoboxes protein 2.";
RL   Submitted (OCT-2006) to the PDB data bank.
CC   -!- FUNCTION: Acts as a transcriptional repressor.
CC   -!- SUBUNIT: Forms homodimers. Also forms heterodimers with ZHX1 and
CC       ZHX3. Heterodimerization with ZHX1 is not necessary for repressor
CC       activity. Interacts with NFYA.
CC   -!- SUBCELLULAR LOCATION: Nucleus.
CC   -!- TISSUE SPECIFICITY: Ubiquitously expressed.
CC   -!- SIMILARITY: Belongs to the ZHX family.
CC   -!- SIMILARITY: Contains 2 C2H2-type zinc fingers.
CC   -!- SIMILARITY: Contains 4 homeobox DNA-binding domains.
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution-NoDerivs License
CC   -----------------------------------------------------------------------
DR   EMBL; AB083653; BAC76615.1; -; mRNA.
DR   EMBL; AB020661; BAA74877.2; ALT_INIT; mRNA.
DR   EMBL; BC042145; AAH42145.1; -; mRNA.
DR   IPI; IPI00007256; -.
DR   RefSeq; NP_055758.1; -.
DR   UniGene; Hs.658443; -.
DR   UniGene; Hs.714291; -.
DR   PDB; 2DMP; NMR; -; A=524-599.
DR   PDBsum; 2DMP; -.
DR   IntAct; Q9Y6X8; 1.
DR   STRING; Q9Y6X8; -.
DR   PhosphoSite; Q9Y6X8; -.
DR   PRIDE; Q9Y6X8; -.
DR   Ensembl; ENST00000314393; ENSP00000314709; ENSG00000178764; Homo sapiens.
DR   GeneID; 22882; -.
DR   KEGG; hsa:22882; -.
DR   UCSC; uc003ypk.1; human.
DR   CTD; 22882; -.
DR   GeneCards; GC08P123863; -.
DR   H-InvDB; HIX0007759; -.
DR   HGNC; HGNC:18513; ZHX2.
DR   MIM; 609185; gene.
DR   PharmGKB; PA128394591; -.
DR   HOGENOM; Q9Y6X8; -.
DR   HOVERGEN; Q9Y6X8; -.
DR   OMA; LNEFTEH; -.
DR   NextBio; 43461; -.
DR   ArrayExpress; Q9Y6X8; -.
DR   Bgee; Q9Y6X8; -.
DR   CleanEx; HS_ZHX2; -.
DR   Genevestigator; Q9Y6X8; -.
DR   GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR   GO; GO:0005515; F:protein binding; IPI:UniProtKB.
DR   GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR   GO; GO:0003700; F:transcription factor activity; IDA:UniProtKB.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0006350; P:transcription; IEA:UniProtKB-KW.
DR   InterPro; IPR001356; Homeobox.
DR   InterPro; IPR017970; Homeobox_CS.
DR   InterPro; IPR012287; Homeodomain-rel.
DR   InterPro; IPR007087; Znf_C2H2.
DR   InterPro; IPR015880; Znf_C2H2-like.
DR   Gene3D; G3DSA:1.10.10.60; Homeodomain-rel; 3.
DR   Pfam; PF00046; Homeobox; 3.
DR   SMART; SM00389; HOX; 4.
DR   SMART; SM00355; ZnF_C2H2; 2.
DR   PROSITE; PS00027; HOMEOBOX_1; FALSE_NEG.
DR   PROSITE; PS50071; HOMEOBOX_2; 3.
DR   PROSITE; PS00028; ZINC_FINGER_C2H2_1; FALSE_NEG.
DR   PROSITE; PS50157; ZINC_FINGER_C2H2_2; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Complete proteome; DNA-binding; Homeobox; Metal-binding;
KW   Nucleus; Phosphoprotein; Polymorphism; Repeat; Repressor;
KW   Transcription; Transcription regulation; Zinc; Zinc-finger.
FT   CHAIN         1    837       Zinc fingers and homeoboxes protein 2.
FT                                /FTId=PRO_0000049391.
FT   ZN_FING      78    101       C2H2-type 1.
FT   ZN_FING     110    133       C2H2-type 2.
FT   DNA_BIND    263    324       Homeobox 1.
FT   DNA_BIND    439    501       Homeobox 2.
FT   DNA_BIND    530    591       Homeobox 3.
FT   DNA_BIND    628    690       Homeobox 4.
FT   REGION      195    358       Required for homodimerization.
FT   REGION      263    497       Required for interaction with NFYA.
FT   REGION      263    446       Required for repressor activity.
FT   REGION      317    446       Required for nuclear localization.
FT   MOD_RES      16     16       Phosphoserine (By similarity).
FT   VARIANT     357    357       V -> M (in dbSNP:rs9649951).
FT                                /FTId=VAR_049594.
FT   VARIANT     649    649       R -> K (in dbSNP:rs35319449).
FT                                /FTId=VAR_049595.
FT   VARIANT     779    779       G -> S (in dbSNP:rs3802264).
FT                                /FTId=VAR_049596.
FT   HELIX       541    550
FT   HELIX       556    566
FT   HELIX       570    585
SQ   SEQUENCE   837 AA;  92307 MW;  BE377ADF76D987D2 CRC64;
     MASKRKSTTP CMVRTSQVVE QDVPEEVDRA KEKGIGTPQP DVAKDSWAAE LENSSKENEV
     IEVKSMGESQ SKKLQGGYEC KYCPYSTQNL NEFTEHVDMQ HPNVILNPLY VCAECNFTTK
     KYDSLSDHNS KFHPGEANFK LKLIKRNNQT VLEQSIETTN HVVSITTSGP GTGDSDSGIS
     VSKTPIMKPG KPKADAKKVP KKPEEITPEN HVEGTARLVT DTAEILSRLG GVELLQDTLG
     HVMPSVQLPP NINLVPKVPV PLNTTKYNSA LDTNATMINS FNKFPYPTQA ELSWLTAASK
     HPEEHIRIWF ATQRLKHGIS WSPEEVEEAR KKMFNGTIQS VPPTITVLPA QLAPTKVTQP
     ILQTALPCQI LGQTSLVLTQ VTSGSTTVSC SPITLAVAGV TNHGQKRPLV TPQAAPEPKR
     PHIAQVPEPP PKVANPPLTP ASDRKKTKEQ IAHLKASFLQ SQFPDDAEVY RLIEVTGLAR
     SEIKKWFSDH RYRCQRGIVH ITSESLAKDQ LAIAASRHGR TYHAYPDFAP QKFKEKTQGQ
     VKILEDSFLK SSFPTQAELD RLRVETKLSR REIDSWFSER RKLRDSMEQA VLDSMGSGKK
     GQDVGAPNGA LSRLDQLSGA QLTSSLPSPS PAIAKSQEQV HLLRSTFART QWPTPQEYDQ
     LAAKTGLVRT EIVRWFKENR CLLKTGTVKW MEQYQHQPMA DDHGYDAVAR KATKPMAESP
     KNGGDVVPQY YKDPKKLCEE DLEKLVTRVK VGSEPAKDCL PAKPSEATSD RSEGSSRDGQ
     GSDENEESSV VDYVEVTVGE EDAISDRSDS WSQAAAEGVS ELAESDSDCV PAEAGQA
//