Q9Y6X8 (ZHX2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Zinc fingers and homeoboxes protein 2

Alternative name(s):
Alpha-fetoprotein regulator 1
Short name=AFP regulator 1
Regulator of AFP
Zinc finger and homeodomain protein 2

Gene names

Name:	ZHX2
Synonyms:	AFR1, KIAA0854, RAF

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	837 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts as a transcriptional repressor. Ref.1
Subunit structure	Forms homodimers. Also forms heterodimers with ZHX1 and ZHX3. Heterodimerization with ZHX1 is not necessary for repressor activity. Interacts with NFYA. Ref.1 UniProtKB Q8C0C0
Subcellular location	Nucleus Ref.1.
Tissue specificity	Ubiquitously expressed. Ref.1
Sequence similarities	Belongs to the ZHX family. Contains 2 C2H2-type zinc fingers. Contains 4 homeobox DNA-binding domains.
Sequence caution	The sequence BAA74877.2 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Polymorphism
Domain	Homeobox Repeat Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Repressor
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from direct assay. Source: HPA nucleus Inferred from direct assay Ref.1. Source: UniProtKB plasma membrane Inferred from direct assay. Source: HPA
Molecular function	protein binding Inferred from physical interaction Ref.1. Source: UniProtKB sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding transcription factor activity Inferred from direct assay Ref.1. Source: UniProtKB zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 837

837

Zinc fingers and homeoboxes protein 2

PRO_0000049391

Regions

Zinc finger

78 – 101

24

C2H2-type 1

Zinc finger

110 – 133

24

C2H2-type 2

DNA binding

263 – 324

62

Homeobox 1

DNA binding

439 – 501

63

Homeobox 2

DNA binding

530 – 591

62

Homeobox 3

DNA binding

628 – 690

63

Homeobox 4

Region

195 – 358

164

Required for homodimerization Ref.1

Region

263 – 497

235

Required for interaction with NFYA Ref.1

Region

263 – 446

184

Required for repressor activity Ref.1

Region

317 – 446

130

Required for nuclear localization Ref.1

Amino acid modifications

Modified residue

16

1

Phosphoserine By similarity

Modified residue

37

1

Phosphothreonine Ref.5

Natural variations

Natural variant

357

1

V → M.
Corresponds to variant rs9649951 [ dbSNP | Ensembl ].

VAR_049594

Natural variant

649

1

R → K.
Corresponds to variant rs35319449 [ dbSNP | Ensembl ].

VAR_049595

Natural variant

779

1

G → S.
Corresponds to variant rs3802264 [ dbSNP | Ensembl ].

VAR_049596

Secondary structure

1

.

.

.

.

.

.

.

837

Helix Strand Turn

Details...

Helix

541 – 550

10

Helix

556 – 566

11

Helix

570 – 585

16

Sequences

	Sequence		Length	Mass (Da)
	Q9Y6X8 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: BE377ADF76D987D2	FASTA	837	92,307
	10 20 30 40 50 60 MASKRKSTTP CMVRTSQVVE QDVPEEVDRA KEKGIGTPQP DVAKDSWAAE LENSSKENEV 70 80 90 100 110 120 IEVKSMGESQ SKKLQGGYEC KYCPYSTQNL NEFTEHVDMQ HPNVILNPLY VCAECNFTTK 130 140 150 160 170 180 KYDSLSDHNS KFHPGEANFK LKLIKRNNQT VLEQSIETTN HVVSITTSGP GTGDSDSGIS 190 200 210 220 230 240 VSKTPIMKPG KPKADAKKVP KKPEEITPEN HVEGTARLVT DTAEILSRLG GVELLQDTLG 250 260 270 280 290 300 HVMPSVQLPP NINLVPKVPV PLNTTKYNSA LDTNATMINS FNKFPYPTQA ELSWLTAASK 310 320 330 340 350 360 HPEEHIRIWF ATQRLKHGIS WSPEEVEEAR KKMFNGTIQS VPPTITVLPA QLAPTKVTQP 370 380 390 400 410 420 ILQTALPCQI LGQTSLVLTQ VTSGSTTVSC SPITLAVAGV TNHGQKRPLV TPQAAPEPKR 430 440 450 460 470 480 PHIAQVPEPP PKVANPPLTP ASDRKKTKEQ IAHLKASFLQ SQFPDDAEVY RLIEVTGLAR 490 500 510 520 530 540 SEIKKWFSDH RYRCQRGIVH ITSESLAKDQ LAIAASRHGR TYHAYPDFAP QKFKEKTQGQ 550 560 570 580 590 600 VKILEDSFLK SSFPTQAELD RLRVETKLSR REIDSWFSER RKLRDSMEQA VLDSMGSGKK 610 620 630 640 650 660 GQDVGAPNGA LSRLDQLSGA QLTSSLPSPS PAIAKSQEQV HLLRSTFART QWPTPQEYDQ 670 680 690 700 710 720 LAAKTGLVRT EIVRWFKENR CLLKTGTVKW MEQYQHQPMA DDHGYDAVAR KATKPMAESP 730 740 750 760 770 780 KNGGDVVPQY YKDPKKLCEE DLEKLVTRVK VGSEPAKDCL PAKPSEATSD RSEGSSRDGQ 790 800 810 820 830 GSDENEESSV VDYVEVTVGE EDAISDRSDS WSQAAAEGVS ELAESDSDCV PAEAGQA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Zinc-fingers and homeoboxes (ZHX) 2, a novel member of the ZHX family, functions as a transcriptional repressor." Kawata H., Yamada K., Shou Z., Mizutani T., Yazawa T., Yoshino M., Sekiguchi T., Kajitani T., Miyamoto K. Biochem. J. 373:747-757(2003) [PubMed: 12741956] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, HOMODIMERIZATION, HETERODIMERIZATION WITH ZHX1, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH NFYA. Tissue: Testis.
[2]	"Prediction of the coding sequences of unidentified human genes. XII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Ishikawa K., Suyama M., Kikuno R., Hirosawa M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O. DNA Res. 5:355-364(1998) [PubMed: 10048485] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[3]	Ohara O., Suyama M., Kikuno R., Nagase T., Ishikawa K. Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lymph.
[5]	"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach." Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S. Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-37, MASS SPECTROMETRY. Tissue: Embryonic kidney.
[6]	"Solution structure of the third homeobox domain of zinc fingers and homeoboxes protein 2." RIKEN structural genomics initiative (RSGI) Submitted (OCT-2006) to the PDB data bank Cited for: STRUCTURE BY NMR OF 524-601.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AB083653 mRNA. Translation: BAC76615.1.
AB020661 mRNA. Translation: BAA74877.2. Different initiation.
BC042145 mRNA. Translation: AAH42145.1.

IPI

IPI00007256.

RefSeq

NP_055758.1. NM_014943.3.

UniGene

Hs.377090.
Hs.658443.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2DMP	NMR	-	A	524-599	[»]
3NAU	X-ray	2.70	A/B	444-501	[»]

ProteinModelPortal

Q9Y6X8.

SMR

Q9Y6X8. Positions 70-156, 275-316, 446-501, 524-593, 631-698.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q9Y6X8. 24 interactions.

MINT

MINT-2869930.

STRING

Q9Y6X8.

PTM databases

PhosphoSite

Q9Y6X8.

Polymorphism databases

DMDM

44888553.

Proteomic databases

PRIDE

Q9Y6X8.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000314393; ENSP00000314709; ENSG00000178764.

GeneID

22882.

KEGG

hsa:22882.

UCSC

uc003ypk.1. human.

Organism-specific databases

CTD

22882.

GeneCards

GC08P123793.

H-InvDB

HIX0007759.

HGNC

HGNC:18513. ZHX2.

HPA

HPA006769.
HPA006996.

MIM

609185. gene.

neXtProt

NX_Q9Y6X8.

PharmGKB

PA128394591.

HUGE

Search...

GenAtlas

Search...

Phylogenomic databases

eggNOG

prNOG08893.

GeneTree

ENSGT00530000063149.

HOGENOM

HBG716153.

HOVERGEN

HBG007920.

InParanoid

Q9Y6X8.

OMA

ATQRLKH.

OrthoDB

EOG4STS45.

PhylomeDB

Q9Y6X8.

Gene expression databases

ArrayExpress

Q9Y6X8.

Bgee

Q9Y6X8.

CleanEx

HS_ZHX2.

Genevestigator

Q9Y6X8.

Family and domain databases

InterPro

IPR001356. Homeobox.
IPR009057. Homeodomain-like.
IPR012287. Homeodomain-rel.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
[Graphical view]

Gene3D

G3DSA:1.10.10.60. Homeodomain-rel. 4 hits.

Pfam

PF00046. Homeobox. 3 hits.
[Graphical view]

SMART

SM00389. HOX. 4 hits.
SM00355. ZnF_C2H2. 2 hits.
[Graphical view]

SUPFAM

SSF46689. Homeodomain_like. 4 hits.

PROSITE

PS00027. HOMEOBOX_1. False negative.
PS50071. HOMEOBOX_2. 3 hits.
PS00028. ZINC_FINGER_C2H2_1. False negative.
PS50157. ZINC_FINGER_C2H2_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

43461.

SOURCE

Search...

Entry information

Entry name

ZHX2_HUMAN

Accession

Primary (citable) accession number: Q9Y6X8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2004
Last sequence update:	November 1, 1999
Last modified:	January 25, 2012
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.