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Protein

Zinc fingers and homeoboxes protein 2

Gene

ZHX2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a transcriptional repressor. Represses the promoter activity of the CDC25C gene stimulated by NFYA.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri78 – 10124C2H2-type 1PROSITE-ProRule annotationCuratedAdd
BLAST
Zinc fingeri110 – 13324C2H2-type 2PROSITE-ProRule annotationCuratedAdd
BLAST
DNA bindingi263 – 32462Homeobox 1PROSITE-ProRule annotationCuratedAdd
BLAST
DNA bindingi439 – 50163Homeobox 2PROSITE-ProRule annotationCuratedAdd
BLAST
DNA bindingi530 – 59162Homeobox 3PROSITE-ProRule annotationCuratedAdd
BLAST
DNA bindingi628 – 69063Homeobox 4PROSITE-ProRule annotationCuratedAdd
BLAST

GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • identical protein binding Source: IntAct
  • metal ion binding Source: UniProtKB-KW
  • protein heterodimerization activity Source: UniProtKB
  • protein homodimerization activity Source: UniProtKB
  • transcription corepressor activity Source: UniProtKB
  • transcription factor activity, sequence-specific DNA binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc fingers and homeoboxes protein 2
Alternative name(s):
Alpha-fetoprotein regulator 1
Short name:
AFP regulator 1
Regulator of AFP
Zinc finger and homeodomain protein 2
Gene namesi
Name:ZHX2
Synonyms:AFR1, KIAA0854, RAF
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 8

Organism-specific databases

HGNCiHGNC:18513. ZHX2.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: HPA
  • nucleoplasm Source: HPA
  • nucleus Source: UniProtKB
  • plasma membrane Source: HPA
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA128394591.

Polymorphism and mutation databases

BioMutaiZHX2.
DMDMi44888553.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 837837Zinc fingers and homeoboxes protein 2PRO_0000049391Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei37 – 371PhosphothreonineCombined sources
Modified residuei207 – 2071PhosphothreonineCombined sources
Modified residuei825 – 8251PhosphoserineBy similarity
Modified residuei827 – 8271PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9Y6X8.
MaxQBiQ9Y6X8.
PaxDbiQ9Y6X8.
PeptideAtlasiQ9Y6X8.
PRIDEiQ9Y6X8.

PTM databases

iPTMnetiQ9Y6X8.
PhosphoSiteiQ9Y6X8.

Expressioni

Tissue specificityi

Ubiquitously expressed. Expressed in podocytes.2 Publications

Gene expression databases

BgeeiENSG00000178764.
CleanExiHS_ZHX2.
ExpressionAtlasiQ9Y6X8. baseline and differential.
GenevisibleiQ9Y6X8. HS.

Organism-specific databases

HPAiHPA006769.
HPA006996.

Interactioni

Subunit structurei

Forms a homodimer (via homeobox domain). Forms a heterodimer with ZHX1 (via HD1 domain). Forms a heterodimer with ZHX3. Heterodimerization with ZHX1 is not necessary for repressor activity. Interacts (via homeobox domain) with NFYA (via N-terminus).1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-948566,EBI-948566
ZHX1Q9UKY12EBI-948566,EBI-347767

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • protein heterodimerization activity Source: UniProtKB
  • protein homodimerization activity Source: UniProtKB

Protein-protein interaction databases

BioGridi116549. 13 interactions.
IntActiQ9Y6X8. 31 interactions.
MINTiMINT-2869930.
STRINGi9606.ENSP00000314709.

Structurei

Secondary structure

1
837
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi448 – 45912Combined sources
Helixi460 – 4623Combined sources
Helixi466 – 47611Combined sources
Helixi480 – 50021Combined sources
Helixi538 – 55013Combined sources
Helixi556 – 56611Combined sources
Helixi570 – 58516Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2DMPNMR-A524-599[»]
3NAUX-ray2.70A/B444-501[»]
ProteinModelPortaliQ9Y6X8.
SMRiQ9Y6X8. Positions 70-156, 446-501, 524-593, 635-695.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9Y6X8.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni195 – 358164Required for homodimerizationAdd
BLAST
Regioni263 – 497235Required for interaction with NFYAAdd
BLAST
Regioni263 – 446184Required for repressor activityAdd
BLAST
Regioni317 – 446130Required for nuclear localizationAdd
BLAST

Sequence similaritiesi

Belongs to the ZHX family.Curated
Contains 2 C2H2-type zinc fingers.PROSITE-ProRule annotationCurated
Contains 4 homeobox DNA-binding domains.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri78 – 10124C2H2-type 1PROSITE-ProRule annotationCuratedAdd
BLAST
Zinc fingeri110 – 13324C2H2-type 2PROSITE-ProRule annotationCuratedAdd
BLAST

Keywords - Domaini

Homeobox, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiENOG410IHET. Eukaryota.
ENOG410Z8A2. LUCA.
GeneTreeiENSGT00530000063149.
HOGENOMiHOG000013231.
HOVERGENiHBG007920.
InParanoidiQ9Y6X8.
OMAiFKENRCL.
OrthoDBiEOG091G02WT.
PhylomeDBiQ9Y6X8.
TreeFamiTF333363.

Family and domain databases

Gene3Di1.10.10.60. 4 hits.
InterProiIPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
[Graphical view]
PfamiPF00046. Homeobox. 3 hits.
[Graphical view]
SMARTiSM00389. HOX. 4 hits.
SM00355. ZnF_C2H2. 2 hits.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 4 hits.
PROSITEiPS50071. HOMEOBOX_2. 3 hits.
PS50157. ZINC_FINGER_C2H2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9Y6X8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASKRKSTTP CMVRTSQVVE QDVPEEVDRA KEKGIGTPQP DVAKDSWAAE
60 70 80 90 100
LENSSKENEV IEVKSMGESQ SKKLQGGYEC KYCPYSTQNL NEFTEHVDMQ
110 120 130 140 150
HPNVILNPLY VCAECNFTTK KYDSLSDHNS KFHPGEANFK LKLIKRNNQT
160 170 180 190 200
VLEQSIETTN HVVSITTSGP GTGDSDSGIS VSKTPIMKPG KPKADAKKVP
210 220 230 240 250
KKPEEITPEN HVEGTARLVT DTAEILSRLG GVELLQDTLG HVMPSVQLPP
260 270 280 290 300
NINLVPKVPV PLNTTKYNSA LDTNATMINS FNKFPYPTQA ELSWLTAASK
310 320 330 340 350
HPEEHIRIWF ATQRLKHGIS WSPEEVEEAR KKMFNGTIQS VPPTITVLPA
360 370 380 390 400
QLAPTKVTQP ILQTALPCQI LGQTSLVLTQ VTSGSTTVSC SPITLAVAGV
410 420 430 440 450
TNHGQKRPLV TPQAAPEPKR PHIAQVPEPP PKVANPPLTP ASDRKKTKEQ
460 470 480 490 500
IAHLKASFLQ SQFPDDAEVY RLIEVTGLAR SEIKKWFSDH RYRCQRGIVH
510 520 530 540 550
ITSESLAKDQ LAIAASRHGR TYHAYPDFAP QKFKEKTQGQ VKILEDSFLK
560 570 580 590 600
SSFPTQAELD RLRVETKLSR REIDSWFSER RKLRDSMEQA VLDSMGSGKK
610 620 630 640 650
GQDVGAPNGA LSRLDQLSGA QLTSSLPSPS PAIAKSQEQV HLLRSTFART
660 670 680 690 700
QWPTPQEYDQ LAAKTGLVRT EIVRWFKENR CLLKTGTVKW MEQYQHQPMA
710 720 730 740 750
DDHGYDAVAR KATKPMAESP KNGGDVVPQY YKDPKKLCEE DLEKLVTRVK
760 770 780 790 800
VGSEPAKDCL PAKPSEATSD RSEGSSRDGQ GSDENEESSV VDYVEVTVGE
810 820 830
EDAISDRSDS WSQAAAEGVS ELAESDSDCV PAEAGQA
Length:837
Mass (Da):92,307
Last modified:November 1, 1999 - v1
Checksum:iBE377ADF76D987D2
GO

Sequence cautioni

The sequence BAA74877 differs from that shown. Reason: Erroneous initiation. Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti357 – 3571V → M.
Corresponds to variant rs9649951 [ dbSNP | Ensembl ].
VAR_049594
Natural varianti649 – 6491R → K.
Corresponds to variant rs35319449 [ dbSNP | Ensembl ].
VAR_049595
Natural varianti779 – 7791G → S.
Corresponds to variant rs3802264 [ dbSNP | Ensembl ].
VAR_049596

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB083653 mRNA. Translation: BAC76615.1.
AB020661 mRNA. Translation: BAA74877.2. Different initiation.
BC042145 mRNA. Translation: AAH42145.1.
CCDSiCCDS6336.1.
RefSeqiNP_055758.1. NM_014943.3.
XP_005250893.1. XM_005250836.4.
XP_005250894.1. XM_005250837.4.
XP_011515233.1. XM_011516931.2.
XP_011515234.1. XM_011516932.2.
UniGeneiHs.377090.
Hs.658443.

Genome annotation databases

EnsembliENST00000314393; ENSP00000314709; ENSG00000178764.
GeneIDi22882.
KEGGihsa:22882.
UCSCiuc003ypk.2. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB083653 mRNA. Translation: BAC76615.1.
AB020661 mRNA. Translation: BAA74877.2. Different initiation.
BC042145 mRNA. Translation: AAH42145.1.
CCDSiCCDS6336.1.
RefSeqiNP_055758.1. NM_014943.3.
XP_005250893.1. XM_005250836.4.
XP_005250894.1. XM_005250837.4.
XP_011515233.1. XM_011516931.2.
XP_011515234.1. XM_011516932.2.
UniGeneiHs.377090.
Hs.658443.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2DMPNMR-A524-599[»]
3NAUX-ray2.70A/B444-501[»]
ProteinModelPortaliQ9Y6X8.
SMRiQ9Y6X8. Positions 70-156, 446-501, 524-593, 635-695.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116549. 13 interactions.
IntActiQ9Y6X8. 31 interactions.
MINTiMINT-2869930.
STRINGi9606.ENSP00000314709.

PTM databases

iPTMnetiQ9Y6X8.
PhosphoSiteiQ9Y6X8.

Polymorphism and mutation databases

BioMutaiZHX2.
DMDMi44888553.

Proteomic databases

EPDiQ9Y6X8.
MaxQBiQ9Y6X8.
PaxDbiQ9Y6X8.
PeptideAtlasiQ9Y6X8.
PRIDEiQ9Y6X8.

Protocols and materials databases

DNASUi22882.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000314393; ENSP00000314709; ENSG00000178764.
GeneIDi22882.
KEGGihsa:22882.
UCSCiuc003ypk.2. human.

Organism-specific databases

CTDi22882.
GeneCardsiZHX2.
HGNCiHGNC:18513. ZHX2.
HPAiHPA006769.
HPA006996.
MIMi609185. gene.
neXtProtiNX_Q9Y6X8.
PharmGKBiPA128394591.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IHET. Eukaryota.
ENOG410Z8A2. LUCA.
GeneTreeiENSGT00530000063149.
HOGENOMiHOG000013231.
HOVERGENiHBG007920.
InParanoidiQ9Y6X8.
OMAiFKENRCL.
OrthoDBiEOG091G02WT.
PhylomeDBiQ9Y6X8.
TreeFamiTF333363.

Miscellaneous databases

ChiTaRSiZHX2. human.
EvolutionaryTraceiQ9Y6X8.
GeneWikiiZHX2.
GenomeRNAii22882.
PROiQ9Y6X8.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000178764.
CleanExiHS_ZHX2.
ExpressionAtlasiQ9Y6X8. baseline and differential.
GenevisibleiQ9Y6X8. HS.

Family and domain databases

Gene3Di1.10.10.60. 4 hits.
InterProiIPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
[Graphical view]
PfamiPF00046. Homeobox. 3 hits.
[Graphical view]
SMARTiSM00389. HOX. 4 hits.
SM00355. ZnF_C2H2. 2 hits.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 4 hits.
PROSITEiPS50071. HOMEOBOX_2. 3 hits.
PS50157. ZINC_FINGER_C2H2_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiZHX2_HUMAN
AccessioniPrimary (citable) accession number: Q9Y6X8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: November 1, 1999
Last modified: September 7, 2016
This is version 141 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.