Zinc fingers and homeoboxes protein 2

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Reviewed, UniProtKB/Swiss-Prot Q9Y6X8 (ZHX2_HUMAN)

Last modified November 3, 2009. Version 73. History...

Clusters with 100%, 90%, 50% identity |

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Zinc fingers and homeoboxes protein 2
Alternative name(s):
    Zinc finger and homeodomain protein 2
    Alpha-fetoprotein regulator 1
      Short name=AFP regulator 1
    Regulator of AFP

Gene names

Name:	ZHX2
Synonyms:	AFR1, KIAA0854, RAF

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length	837 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Acts as a transcriptional repressor. Ref.1
Subunit structure	Forms homodimers. Also forms heterodimers with ZHX1 and ZHX3. Heterodimerization with ZHX1 is not necessary for repressor activity. Interacts with NFYA. Ref.1 UniProtKB Q8C0C0
Subcellular location	Nucleus. Ref.1
Tissue specificity	Ubiquitously expressed. Ref.1
Sequence similarities	Belongs to the ZHX family. Contains 2 C2H2-type zinc fingers. Contains 4 homeobox DNA-binding domains.

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Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Polymorphism
Domain	Homeobox Repeat Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Repressor
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Ref.1 Inferred from direct assay. Source: UniProtKB
Molecular function	protein binding Ref.1 Inferred from physical interaction. Source: UniProtKB sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro transcription factor activity Ref.1 Inferred from direct assay. Source: UniProtKB zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 837

837

Zinc fingers and homeoboxes protein 2

PRO_0000049391

Regions

Zinc finger

78 – 101

C2H2-type 1

Zinc finger

110 – 133

C2H2-type 2

DNA binding

263 – 324

Homeobox 1

DNA binding

439 – 501

Homeobox 2

DNA binding

530 – 591

Homeobox 3

DNA binding

628 – 690

Homeobox 4

Region

195 – 358

164

Required for homodimerization Ref.1

Region

263 – 497

235

Required for interaction with NFYA Ref.1

Region

263 – 446

184

Required for repressor activity Ref.1

Region

317 – 446

130

Required for nuclear localization Ref.1

Amino acid modifications

Modified residue

Phosphoserine By similarity

Natural variations

Natural variant

357

V → M: dbSNP rs9649951.

VAR_049594

Natural variant

649

R → K: dbSNP rs35319449.

VAR_049595

Natural variant

779

G → S: dbSNP rs3802264.

VAR_049596

Secondary structure

837

Helix Strand Turn

Details...

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Sequences

Sequence

Length

Mass (Da)

Tools

Q9Y6X8-1 [UniParc].

Last modified November 1, 1999. Version 1.
Checksum: BE377ADF76D987D2
Show »

FASTA

837

92,307

        10         20         30         40         50         60 
MASKRKSTTP CMVRTSQVVE QDVPEEVDRA KEKGIGTPQP DVAKDSWAAE LENSSKENEV 

        70         80         90        100        110        120 
IEVKSMGESQ SKKLQGGYEC KYCPYSTQNL NEFTEHVDMQ HPNVILNPLY VCAECNFTTK 

       130        140        150        160        170        180 
KYDSLSDHNS KFHPGEANFK LKLIKRNNQT VLEQSIETTN HVVSITTSGP GTGDSDSGIS 

       190        200        210        220        230        240 
VSKTPIMKPG KPKADAKKVP KKPEEITPEN HVEGTARLVT DTAEILSRLG GVELLQDTLG 

       250        260        270        280        290        300 
HVMPSVQLPP NINLVPKVPV PLNTTKYNSA LDTNATMINS FNKFPYPTQA ELSWLTAASK 

       310        320        330        340        350        360 
HPEEHIRIWF ATQRLKHGIS WSPEEVEEAR KKMFNGTIQS VPPTITVLPA QLAPTKVTQP 

       370        380        390        400        410        420 
ILQTALPCQI LGQTSLVLTQ VTSGSTTVSC SPITLAVAGV TNHGQKRPLV TPQAAPEPKR 

       430        440        450        460        470        480 
PHIAQVPEPP PKVANPPLTP ASDRKKTKEQ IAHLKASFLQ SQFPDDAEVY RLIEVTGLAR 

       490        500        510        520        530        540 
SEIKKWFSDH RYRCQRGIVH ITSESLAKDQ LAIAASRHGR TYHAYPDFAP QKFKEKTQGQ 

       550        560        570        580        590        600 
VKILEDSFLK SSFPTQAELD RLRVETKLSR REIDSWFSER RKLRDSMEQA VLDSMGSGKK 

       610        620        630        640        650        660 
GQDVGAPNGA LSRLDQLSGA QLTSSLPSPS PAIAKSQEQV HLLRSTFART QWPTPQEYDQ 

       670        680        690        700        710        720 
LAAKTGLVRT EIVRWFKENR CLLKTGTVKW MEQYQHQPMA DDHGYDAVAR KATKPMAESP 

       730        740        750        760        770        780 
KNGGDVVPQY YKDPKKLCEE DLEKLVTRVK VGSEPAKDCL PAKPSEATSD RSEGSSRDGQ 

       790        800        810        820        830 
GSDENEESSV VDYVEVTVGE EDAISDRSDS WSQAAAEGVS ELAESDSDCV PAEAGQA

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Zinc-fingers and homeoboxes (ZHX) 2, a novel member of the ZHX family, functions as a transcriptional repressor." Kawata H., Yamada K., Shou Z., Mizutani T., Yazawa T., Yoshino M., Sekiguchi T., Kajitani T., Miyamoto K. Biochem. J. 373:747-757(2003) [PubMed: 12741956] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, HOMODIMERIZATION, HETERODIMERIZATION WITH ZHX1, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH NFYA. Tissue: Testis.
[2]	"Prediction of the coding sequences of unidentified human genes. XII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Ishikawa K., Suyama M., Kikuno R., Hirosawa M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O. DNA Res. 5:355-364(1998) [PubMed: 10048485] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[3]	Ohara O., Suyama M., Kikuno R., Nagase T., Ishikawa K. Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lymph.
[5]	"Solution structure of the third homeobox domain of zinc fingers and homeoboxes protein 2." RIKEN structural genomics initiative (RSGI) Submitted (OCT-2006) to the PDB data bank Cited for: STRUCTURE BY NMR OF 524-601.
+	Additional computationally mapped references.

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Cross-references

Sequence databases

AB083653 mRNA. Translation: BAC76615.1.
AB020661 mRNA. Translation: BAA74877.2. Different initiation.
BC042145 mRNA. Translation: AAH42145.1.

IPI

IPI00007256.

RefSeq

NP_055758.1.

UniGene

Hs.658443
Hs.714291

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2DMP	NMR	-	A	524-599	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

Q9Y6X8. 1 interaction.

STRING

Q9Y6X8.

PTM databases

PhosphoSite

Q9Y6X8.

Proteomic databases

PRIDE

Q9Y6X8.

Genome annotation databases

Ensembl

ENST00000314393; ENSP00000314709; ENSG00000178764; Homo sapiens. [Genome view]

GeneID

22882.

KEGG

hsa:22882.

UCSC

uc003ypk.1. human.

Organism-specific databases

CTD

22882.

GeneCards

GC08P123863.

H-InvDB

HIX0007759.

HGNC

HGNC:18513. ZHX2.

MIM

609185. gene.

PharmGKB

PA128394591.

HUGE

Search...

GenAtlas

Search...

Phylogenomic databases

HOGENOM

Q9Y6X8.

HOVERGEN

Q9Y6X8.

OMA

LNEFTEH.

Gene expression databases

ArrayExpress

Q9Y6X8.

Bgee

Q9Y6X8.

CleanEx

HS_ZHX2.

Genevestigator

Q9Y6X8.

Family and domain databases

InterPro

IPR001356. Homeobox.
IPR017970. Homeobox_CS.
IPR012287. Homeodomain-rel.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
[Graphical view]

Gene3D

G3DSA:1.10.10.60. Homeodomain-rel. 3 hits.

Pfam

PF00046. Homeobox. 3 hits.
[Graphical view]

SMART

SM00389. HOX. 4 hits.
SM00355. ZnF_C2H2. 2 hits.
[Graphical view]

PROSITE

PS00027. HOMEOBOX_1. False negative.
PS50071. HOMEOBOX_2. 3 hits.
PS00028. ZINC_FINGER_C2H2_1. False negative.
PS50157. ZINC_FINGER_C2H2_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

43461.

SOURCE

Search...

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Entry information

Entry name

ZHX2_HUMAN

Accession

Primary (citable) accession number: Q9Y6X8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2004
Last sequence update:	November 1, 1999
Last modified:	November 3, 2009
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

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