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Protein

Zinc fingers and homeoboxes protein 2

Gene

ZHX2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a transcriptional repressor. Represses the promoter activity of the CDC25C gene stimulated by NFYA.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri78 – 101C2H2-type 1PROSITE-ProRule annotationCuratedAdd BLAST24
Zinc fingeri110 – 133C2H2-type 2PROSITE-ProRule annotationCuratedAdd BLAST24
DNA bindingi263 – 324Homeobox 1PROSITE-ProRule annotationCuratedAdd BLAST62
DNA bindingi439 – 501Homeobox 2PROSITE-ProRule annotationCuratedAdd BLAST63
DNA bindingi530 – 591Homeobox 3PROSITE-ProRule annotationCuratedAdd BLAST62
DNA bindingi628 – 690Homeobox 4PROSITE-ProRule annotationCuratedAdd BLAST63

GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW
  • protein heterodimerization activity Source: UniProtKB
  • protein homodimerization activity Source: UniProtKB
  • transcription corepressor activity Source: UniProtKB
  • transcription factor activity, sequence-specific DNA binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciZFISH:ENSG00000178764-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc fingers and homeoboxes protein 2
Alternative name(s):
Alpha-fetoprotein regulator 1
Short name:
AFP regulator 1
Regulator of AFP
Zinc finger and homeodomain protein 2
Gene namesi
Name:ZHX2
Synonyms:AFR1, KIAA0854, RAF
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 8

Organism-specific databases

HGNCiHGNC:18513. ZHX2.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: HPA
  • nucleoplasm Source: HPA
  • nucleus Source: UniProtKB
  • plasma membrane Source: HPA
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi22882.
OpenTargetsiENSG00000178764.
PharmGKBiPA128394591.

Polymorphism and mutation databases

BioMutaiZHX2.
DMDMi44888553.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000493911 – 837Zinc fingers and homeoboxes protein 2Add BLAST837

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei37PhosphothreonineCombined sources1
Modified residuei207PhosphothreonineCombined sources1
Modified residuei825PhosphoserineBy similarity1
Modified residuei827PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9Y6X8.
MaxQBiQ9Y6X8.
PaxDbiQ9Y6X8.
PeptideAtlasiQ9Y6X8.
PRIDEiQ9Y6X8.

PTM databases

iPTMnetiQ9Y6X8.
PhosphoSitePlusiQ9Y6X8.

Expressioni

Tissue specificityi

Ubiquitously expressed. Expressed in podocytes.2 Publications

Gene expression databases

BgeeiENSG00000178764.
CleanExiHS_ZHX2.
ExpressionAtlasiQ9Y6X8. baseline and differential.
GenevisibleiQ9Y6X8. HS.

Organism-specific databases

HPAiHPA006769.
HPA006996.

Interactioni

Subunit structurei

Forms a homodimer (via homeobox domain). Forms a heterodimer with ZHX1 (via HD1 domain). Forms a heterodimer with ZHX3. Heterodimerization with ZHX1 is not necessary for repressor activity. Interacts (via homeobox domain) with NFYA (via N-terminus).1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-948566,EBI-948566
ZHX1Q9UKY12EBI-948566,EBI-347767

GO - Molecular functioni

  • protein heterodimerization activity Source: UniProtKB
  • protein homodimerization activity Source: UniProtKB

Protein-protein interaction databases

BioGridi116549. 13 interactors.
IntActiQ9Y6X8. 31 interactors.
MINTiMINT-2869930.
STRINGi9606.ENSP00000314709.

Structurei

Secondary structure

1837
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi448 – 459Combined sources12
Helixi460 – 462Combined sources3
Helixi466 – 476Combined sources11
Helixi480 – 500Combined sources21
Helixi538 – 550Combined sources13
Helixi556 – 566Combined sources11
Helixi570 – 585Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2DMPNMR-A524-599[»]
3NAUX-ray2.70A/B444-501[»]
ProteinModelPortaliQ9Y6X8.
SMRiQ9Y6X8.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9Y6X8.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni195 – 358Required for homodimerizationAdd BLAST164
Regioni263 – 497Required for interaction with NFYA1 PublicationAdd BLAST235
Regioni263 – 446Required for repressor activityAdd BLAST184
Regioni317 – 446Required for nuclear localizationAdd BLAST130

Sequence similaritiesi

Belongs to the ZHX family.Curated
Contains 2 C2H2-type zinc fingers.PROSITE-ProRule annotationCurated
Contains 4 homeobox DNA-binding domains.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri78 – 101C2H2-type 1PROSITE-ProRule annotationCuratedAdd BLAST24
Zinc fingeri110 – 133C2H2-type 2PROSITE-ProRule annotationCuratedAdd BLAST24

Keywords - Domaini

Homeobox, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiENOG410IHET. Eukaryota.
ENOG410Z8A2. LUCA.
GeneTreeiENSGT00530000063149.
HOGENOMiHOG000013231.
HOVERGENiHBG007920.
InParanoidiQ9Y6X8.
OMAiFKENRCL.
OrthoDBiEOG091G02WT.
PhylomeDBiQ9Y6X8.
TreeFamiTF333363.

Family and domain databases

Gene3Di1.10.10.60. 4 hits.
InterProiIPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
[Graphical view]
PfamiPF00046. Homeobox. 3 hits.
[Graphical view]
SMARTiSM00389. HOX. 4 hits.
SM00355. ZnF_C2H2. 2 hits.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 4 hits.
PROSITEiPS50071. HOMEOBOX_2. 3 hits.
PS50157. ZINC_FINGER_C2H2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9Y6X8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASKRKSTTP CMVRTSQVVE QDVPEEVDRA KEKGIGTPQP DVAKDSWAAE
60 70 80 90 100
LENSSKENEV IEVKSMGESQ SKKLQGGYEC KYCPYSTQNL NEFTEHVDMQ
110 120 130 140 150
HPNVILNPLY VCAECNFTTK KYDSLSDHNS KFHPGEANFK LKLIKRNNQT
160 170 180 190 200
VLEQSIETTN HVVSITTSGP GTGDSDSGIS VSKTPIMKPG KPKADAKKVP
210 220 230 240 250
KKPEEITPEN HVEGTARLVT DTAEILSRLG GVELLQDTLG HVMPSVQLPP
260 270 280 290 300
NINLVPKVPV PLNTTKYNSA LDTNATMINS FNKFPYPTQA ELSWLTAASK
310 320 330 340 350
HPEEHIRIWF ATQRLKHGIS WSPEEVEEAR KKMFNGTIQS VPPTITVLPA
360 370 380 390 400
QLAPTKVTQP ILQTALPCQI LGQTSLVLTQ VTSGSTTVSC SPITLAVAGV
410 420 430 440 450
TNHGQKRPLV TPQAAPEPKR PHIAQVPEPP PKVANPPLTP ASDRKKTKEQ
460 470 480 490 500
IAHLKASFLQ SQFPDDAEVY RLIEVTGLAR SEIKKWFSDH RYRCQRGIVH
510 520 530 540 550
ITSESLAKDQ LAIAASRHGR TYHAYPDFAP QKFKEKTQGQ VKILEDSFLK
560 570 580 590 600
SSFPTQAELD RLRVETKLSR REIDSWFSER RKLRDSMEQA VLDSMGSGKK
610 620 630 640 650
GQDVGAPNGA LSRLDQLSGA QLTSSLPSPS PAIAKSQEQV HLLRSTFART
660 670 680 690 700
QWPTPQEYDQ LAAKTGLVRT EIVRWFKENR CLLKTGTVKW MEQYQHQPMA
710 720 730 740 750
DDHGYDAVAR KATKPMAESP KNGGDVVPQY YKDPKKLCEE DLEKLVTRVK
760 770 780 790 800
VGSEPAKDCL PAKPSEATSD RSEGSSRDGQ GSDENEESSV VDYVEVTVGE
810 820 830
EDAISDRSDS WSQAAAEGVS ELAESDSDCV PAEAGQA
Length:837
Mass (Da):92,307
Last modified:November 1, 1999 - v1
Checksum:iBE377ADF76D987D2
GO

Sequence cautioni

The sequence BAA74877 differs from that shown. Reason: Erroneous initiation.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_049594357V → M.Corresponds to variant rs9649951dbSNPEnsembl.1
Natural variantiVAR_049595649R → K.Corresponds to variant rs35319449dbSNPEnsembl.1
Natural variantiVAR_049596779G → S.Corresponds to variant rs3802264dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB083653 mRNA. Translation: BAC76615.1.
AB020661 mRNA. Translation: BAA74877.2. Different initiation.
BC042145 mRNA. Translation: AAH42145.1.
CCDSiCCDS6336.1.
RefSeqiNP_055758.1. NM_014943.3.
XP_005250893.1. XM_005250836.4.
XP_005250894.1. XM_005250837.4.
XP_011515233.1. XM_011516931.2.
XP_011515234.1. XM_011516932.2.
UniGeneiHs.377090.
Hs.658443.

Genome annotation databases

EnsembliENST00000314393; ENSP00000314709; ENSG00000178764.
GeneIDi22882.
KEGGihsa:22882.
UCSCiuc003ypk.2. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB083653 mRNA. Translation: BAC76615.1.
AB020661 mRNA. Translation: BAA74877.2. Different initiation.
BC042145 mRNA. Translation: AAH42145.1.
CCDSiCCDS6336.1.
RefSeqiNP_055758.1. NM_014943.3.
XP_005250893.1. XM_005250836.4.
XP_005250894.1. XM_005250837.4.
XP_011515233.1. XM_011516931.2.
XP_011515234.1. XM_011516932.2.
UniGeneiHs.377090.
Hs.658443.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2DMPNMR-A524-599[»]
3NAUX-ray2.70A/B444-501[»]
ProteinModelPortaliQ9Y6X8.
SMRiQ9Y6X8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116549. 13 interactors.
IntActiQ9Y6X8. 31 interactors.
MINTiMINT-2869930.
STRINGi9606.ENSP00000314709.

PTM databases

iPTMnetiQ9Y6X8.
PhosphoSitePlusiQ9Y6X8.

Polymorphism and mutation databases

BioMutaiZHX2.
DMDMi44888553.

Proteomic databases

EPDiQ9Y6X8.
MaxQBiQ9Y6X8.
PaxDbiQ9Y6X8.
PeptideAtlasiQ9Y6X8.
PRIDEiQ9Y6X8.

Protocols and materials databases

DNASUi22882.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000314393; ENSP00000314709; ENSG00000178764.
GeneIDi22882.
KEGGihsa:22882.
UCSCiuc003ypk.2. human.

Organism-specific databases

CTDi22882.
DisGeNETi22882.
GeneCardsiZHX2.
HGNCiHGNC:18513. ZHX2.
HPAiHPA006769.
HPA006996.
MIMi609185. gene.
neXtProtiNX_Q9Y6X8.
OpenTargetsiENSG00000178764.
PharmGKBiPA128394591.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IHET. Eukaryota.
ENOG410Z8A2. LUCA.
GeneTreeiENSGT00530000063149.
HOGENOMiHOG000013231.
HOVERGENiHBG007920.
InParanoidiQ9Y6X8.
OMAiFKENRCL.
OrthoDBiEOG091G02WT.
PhylomeDBiQ9Y6X8.
TreeFamiTF333363.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000178764-MONOMER.

Miscellaneous databases

ChiTaRSiZHX2. human.
EvolutionaryTraceiQ9Y6X8.
GeneWikiiZHX2.
GenomeRNAii22882.
PROiQ9Y6X8.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000178764.
CleanExiHS_ZHX2.
ExpressionAtlasiQ9Y6X8. baseline and differential.
GenevisibleiQ9Y6X8. HS.

Family and domain databases

Gene3Di1.10.10.60. 4 hits.
InterProiIPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
[Graphical view]
PfamiPF00046. Homeobox. 3 hits.
[Graphical view]
SMARTiSM00389. HOX. 4 hits.
SM00355. ZnF_C2H2. 2 hits.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 4 hits.
PROSITEiPS50071. HOMEOBOX_2. 3 hits.
PS50157. ZINC_FINGER_C2H2_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiZHX2_HUMAN
AccessioniPrimary (citable) accession number: Q9Y6X8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: November 1, 1999
Last modified: November 2, 2016
This is version 143 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.