Reviewed, UniProtKB/Swiss-Prot Q9Y6X2 (PIAS3_HUMAN)

Names and origin

Protein names

Recommended name:
    E3 SUMO-protein ligase PIAS3
Alternative name(s):
    Protein inhibitor of activated STAT protein 3

Gene names

Name:

PIAS3

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	628 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway and the steroid hormone signaling pathway. The effects of this transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context.
Pathway	Protein modification; protein sumoylation.
Subunit structure	Binds SUMO1 and UBE2I. Interacts with AR, GFI1, HMGA2, IRF1, MITF, NCOA2, as well as with STAT3, after treatment with IL6, CNTF or OSM and with STAT5, after PRL stimulation By similarity. Interacts with PLAG1.
Subcellular location	Nucleus speckle By similarity.
Tissue specificity	Widely expressed. Ref.4
Induction	By dihydrotestosterone in prostate cancer cells. Ref.5
Domain	The LXXLL motif is a transcriptional coregulator signature.
Post-translational modification	Sumoylated By similarity.
Sequence similarities	Belongs to the PIAS family. Contains 1 SAP domain. Contains 1 SP-RING-type zinc finger.

Ontologies

Keywords
Biological process	Transcription Transcription regulation Ubl conjugation pathway
Cellular component	Nucleus
Coding sequence diversity	Polymorphism
Domain	Zinc-finger
Ligand	Metal-binding Zinc
PTM	Ubl conjugation
Gene Ontology (GO)
Biological process	modification-dependent protein catabolic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nuclear speck Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	nucleic acid binding Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 628	628	E3 SUMO-protein ligase PIAS3		PRO_0000218979
Regions
	Domain	11 – 45	35	SAP
	Zinc finger	303 – 380	78	SP-RING-type
	Region	450 – 460	11	SUMO1-binding By similarity
	Motif	19 – 23	5	LXXLL motif
	Compositional bias	81 – 133	53	Pro-rich
Natural variations
	Natural variant	390	1	S → C: dbSNP rs17354559.		VAR_050535

Sequences

	Sequence		Length	Mass (Da)
	Q9Y6X2-1 [UniParc]. Last modified December 7, 2004. Version 2. Checksum: 7C06EF599D48F87D	FASTA	628	68,017
	10 20 30 40 50 60 MAELGELKHM VMSFRVSELQ VLLGFAGRNK SGRKHELLAK ALHLLKSSCA PSVQMKIKEL 70 80 90 100 110 120 YRRRFPRKTL GPSDLSLLSL PPGTSPVGSP GPLAPIPPTL LAPGTLLGPK REVDMHPPLP 130 140 150 160 170 180 QPVHPDVTMK PLPFYEVYGE LIRPTTLAST SSQRFEEAHF TFALTPQQVQ QILTSREVLP 190 200 210 220 230 240 GAKCDYTIQV QLRFCLCETS CPQEDYFPPN LFVKVNGKLC PLPGYLPPTK NGAEPKRPSR 250 260 270 280 290 300 PINITPLARL SATVPNTIVV NWSSEFGRNY SLSVYLVRQL TAGTLLQKLR AKGIRNPDHS 310 320 330 340 350 360 RALIKEKLTA DPDSEVATTS LRVSLMCPLG KMRLTVPCRA LTCAHLQSFD AALYLQMNEK 370 380 390 400 410 420 KPTWTCPVCD KKAPYESLII DGLFMEILSS CSDCDEIQFM EDGSWCPMKP KKEASEVCPP 430 440 450 460 470 480 PGYGLDGLQY SPVQGGDPSE NKKKVEVIDL TIESSSDEED LPPTKKHCSV TSAAIPALPG 490 500 510 520 530 540 SKGVLTSGHQ PSSVLRSPAM GTLGGDFLSS LPLHEYPPAF PLGADIQGLD LFSFLQTESQ 550 560 570 580 590 600 HYGPSVITSL DEQDALGHFF QYRGTPSHFL GPLAPTLGSS HCSATPAPPP GRVSSIVAPG 610 620 GALREGHGGP LPSGPSLTGC RSDIISLD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and chromosomal assignment of a human gene encoding protein inhibitor of activated STAT3 (PIAS3)." Ueki N., Seki N., Yano K., Saito T., Masuho Y., Muramatsu M.-A. J. Hum. Genet. 44:193-196(1999) [PubMed: 10319586] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 2-628.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Blood and Muscle.
[3]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Blocker H., Heubner D., Hoerlein A., Michel G., Wedler H., Kohrer K., Ottenwalder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 310-628. Tissue: Mammary cancer.
[4]	"Specific inhibition of Stat3 signal transduction by PIAS3." Chung C.D., Liao J., Liu B., Rao X., Jay P., Berta P., Shuai K. Science 278:1803-1805(1997) [PubMed: 9388184] [Abstract] Cited for: TISSUE SPECIFICITY.
[5]	"Protein inhibitor of activated STAT3 regulates androgen receptor signaling in prostate carcinoma cells." Junicho A., Matsuda T., Yamamoto T., Kishi H., Korkmaz K., Saatcioglu F., Fuse H., Muraguchi A. Biochem. Biophys. Res. Commun. 278:9-13(2000) [PubMed: 11071847] [Abstract] Cited for: INTERACTION WITH AR, INDUCTION.
[6]	"The zinc finger protein Gfi-1 can enhance STAT3 signaling by interacting with the STAT3 inhibitor PIAS3." Roedel B., Tavassoli K., Karsunky H., Schmidt T., Bachmann M., Schaper F., Heinrich P., Shuai K., Elsaesser H.-P., Moeroey T. EMBO J. 19:5845-5855(2000) [PubMed: 11060035] [Abstract] Cited for: INTERACTION WITH GFI1.
[7]	"Repression of the transactivating capacity of the oncoprotein PLAG1 by SUMOylation." Van Dyck F., Delvaux E.L.D., Van de Ven W.J.M., Chavez M.V. J. Biol. Chem. 279:36121-36131(2004) [PubMed: 15208321] [Abstract] Cited for: INTERACTION WITH PLAG1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB021868 mRNA. Translation: BAA78533.1. Different initiation. BC001154 mRNA. Translation: AAH01154.1. Different initiation. BC030556 mRNA. Translation: AAH30556.1. Different initiation.
IPI	IPI00005789.
PIR	T34525.
RefSeq	NP_006090.2.
UniGene	Hs.435761
3D structure databases
SMR	Q9Y6X2. Positions 1-65.
ModBase	Search...
Proteomic databases
PRIDE	Q9Y6X2.
Genome annotation databases
Ensembl	ENSG00000131788. Homo sapiens. [Contig view]
GeneID	10401.
KEGG	hsa:10401.
Organism-specific databases
GeneCards	GC01P144287.
H-InvDB	HIX0000976.
HGNC	HGNC:16861. PIAS3.
MIM	605987. gene.
PharmGKB	PA134989011.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q9Y6X2.
HOVERGEN	Q9Y6X2.
OMA	Q9Y6X2. CSVTSAA.
Enzyme and pathway databases
Pathway_Interaction_DB	ar_pathway. Coregulation of Androgen receptor activity. il6_7pathway. IL6-mediated signaling events. smad2_3nuclearpathway. Regulation of nuclear SMAD2/3 signaling.
Gene expression databases
Bgee	Q9Y6X2.
CleanEx	HS_PIAS3.
GermOnline	ENSG00000131788. Homo sapiens.
Family and domain databases
InterPro	IPR003034. SAP_DNA_bd. IPR004181. Znf_MIZ. [Graphical view]
Pfam	PF02037. SAP. 1 hit. PF02891. zf-MIZ. 1 hit. [Graphical view]
SMART	SM00513. SAP. 1 hit. [Graphical view]
PROSITE	PS50800. SAP. 1 hit. PS51044. ZF_SP_RING. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	39416.
SOURCE	Search...

Entry information

Entry name

PIAS3_HUMAN

Accession

Primary (citable) accession number: Q9Y6X2
Secondary accession number(s): Q9UFI3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 5, 2002
Last sequence update:	December 7, 2004
Last modified:	June 16, 2009
This is version 73 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)