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Q9Y6X2

- PIAS3_HUMAN

UniProt

Q9Y6X2 - PIAS3_HUMAN

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Protein

E3 SUMO-protein ligase PIAS3

Gene

PIAS3

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway and the steroid hormone signaling pathway. Involved in regulating STAT3 signaling via inhibiting STAT3 DNA-binding and suppressing cell growth. Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation.2 Publications

Pathwayi

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri303 – 38078SP-RING-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. enzyme binding Source: UniProtKB
  2. nucleic acid binding Source: InterPro
  3. potassium channel regulator activity Source: Ensembl
  4. protein C-terminus binding Source: UniProtKB
  5. SUMO ligase activity Source: Ensembl
  6. zinc ion binding Source: InterPro

GO - Biological processi

  1. positive regulation of gene expression Source: Ensembl
  2. positive regulation of membrane potential Source: Ensembl
  3. positive regulation of protein sumoylation Source: UniProtKB
  4. protein sumoylation Source: UniProtKB
  5. regulation of transcription, DNA-templated Source: UniProtKB-KW
  6. response to hormone Source: Ensembl
  7. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Transcription, Transcription regulation, Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

SignaLinkiQ9Y6X2.
UniPathwayiUPA00886.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 SUMO-protein ligase PIAS3 (EC:6.3.2.-)
Alternative name(s):
Protein inhibitor of activated STAT protein 3
Gene namesi
Name:PIAS3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 1

Organism-specific databases

HGNCiHGNC:16861. PIAS3.

Subcellular locationi

Cytoplasm. Nucleus
Note: Mainly cytoplasmic but shuttles between the nucleus and cytoplasm. Transported to the nucleus on interaction with phosphorylated STAT3. Colocalizes with GFI1 in nuclear dots By similarity.By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. dendrite Source: Ensembl
  3. nucleus Source: UniProtKB-KW
  4. synapse Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134989011.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 628628E3 SUMO-protein ligase PIAS3PRO_0000218979Add
BLAST

Post-translational modificationi

Sumoylated.By similarity

Keywords - PTMi

Ubl conjugation

Proteomic databases

MaxQBiQ9Y6X2.
PaxDbiQ9Y6X2.
PRIDEiQ9Y6X2.

PTM databases

PhosphoSiteiQ9Y6X2.

Expressioni

Tissue specificityi

Isoform 1 is expressed in most tissues except thymus and small intestine. Isoform 3 is expressed only in brain, heart, thymus, muscle, lung, testis, lactating breast and embryonic stem cells.1 Publication

Inductioni

By dihydrotestosterone (DHT) in prostate cancer cells.1 Publication

Gene expression databases

BgeeiQ9Y6X2.
CleanExiHS_PIAS3.
ExpressionAtlasiQ9Y6X2. baseline and differential.
GenevestigatoriQ9Y6X2.

Interactioni

Subunit structurei

Monomer By similarity. Binds SUMO1 and UBE2I. Interacts with BCL11A, HMGA2, IRF1, MITF and NCOA2. Interacts with STAT5; the interaction occurs on stimulation by PRL. Interacts with GFI1; the interaction relieves the inhibitory effect of PIAS3 on STAT3-mediated transcriptional activity By similarity. Interacts with AR, PLAG1 and ZFHX3. Interacts with STAT3; the interaction occurs on stimulation by IL6, CNTF or OSM and inhibits the DNA binding activity of STAT3. Interacts with MTA1.By similarity6 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
MBD1Q9UIS93EBI-2803703,EBI-867196

Protein-protein interaction databases

BioGridi115673. 53 interactions.
DIPiDIP-5969N.
IntActiQ9Y6X2. 25 interactions.
MINTiMINT-149343.
STRINGi9606.ENSP00000376765.

Structurei

Secondary structure

1
628
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi135 – 14713
Beta strandi155 – 1628
Helixi166 – 1738
Beta strandi187 – 19711
Beta strandi212 – 2154
Beta strandi218 – 2203
Helixi245 – 2473
Beta strandi252 – 2543
Beta strandi256 – 2649
Beta strandi268 – 27912
Helixi282 – 29110
Helixi297 – 30711
Beta strandi320 – 3267
Turni328 – 3303
Beta strandi335 – 3406
Helixi351 – 36010
Turni367 – 3693
Helixi375 – 3773
Beta strandi378 – 3814
Helixi382 – 3909
Beta strandi395 – 4006
Beta strandi405 – 4073

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4MVTX-ray2.30A/B/C/D112-467[»]
ProteinModelPortaliQ9Y6X2.
SMRiQ9Y6X2. Positions 1-65, 126-408.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini11 – 4535SAPPROSITE-ProRule annotationAdd
BLAST
Domaini115 – 280166PINITPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni450 – 46011SUMO1-bindingBy similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi19 – 235LXXLL motif

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi81 – 13353Pro-richAdd
BLAST

Domaini

The PINIT domain of PIAS3 is required for STAT3-PIAS3 interaction and for transloaction to the nucleus.
The LXXLL motif is a transcriptional coregulator signature.

Sequence similaritiesi

Belongs to the PIAS family.Curated
Contains 1 PINIT domain.PROSITE-ProRule annotation
Contains 1 SAP domain.PROSITE-ProRule annotation
Contains 1 SP-RING-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri303 – 38078SP-RING-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiNOG125513.
GeneTreeiENSGT00550000074410.
HOGENOMiHOG000230594.
HOVERGENiHBG053598.
InParanoidiQ9Y6X2.
KOiK16064.
OMAiKHMVMSF.
PhylomeDBiQ9Y6X2.
TreeFamiTF323787.

Family and domain databases

Gene3Di1.10.720.30. 1 hit.
InterProiIPR027226. PIAS3.
IPR023321. PINIT.
IPR003034. SAP_dom.
IPR004181. Znf_MIZ.
[Graphical view]
PANTHERiPTHR10782:SF10. PTHR10782:SF10. 1 hit.
PfamiPF14324. PINIT. 1 hit.
PF02891. zf-MIZ. 1 hit.
[Graphical view]
SMARTiSM00513. SAP. 1 hit.
[Graphical view]
PROSITEiPS51466. PINIT. 1 hit.
PS50800. SAP. 1 hit.
PS51044. ZF_SP_RING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9Y6X2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAELGELKHM VMSFRVSELQ VLLGFAGRNK SGRKHELLAK ALHLLKSSCA
60 70 80 90 100
PSVQMKIKEL YRRRFPRKTL GPSDLSLLSL PPGTSPVGSP GPLAPIPPTL
110 120 130 140 150
LAPGTLLGPK REVDMHPPLP QPVHPDVTMK PLPFYEVYGE LIRPTTLAST
160 170 180 190 200
SSQRFEEAHF TFALTPQQVQ QILTSREVLP GAKCDYTIQV QLRFCLCETS
210 220 230 240 250
CPQEDYFPPN LFVKVNGKLC PLPGYLPPTK NGAEPKRPSR PINITPLARL
260 270 280 290 300
SATVPNTIVV NWSSEFGRNY SLSVYLVRQL TAGTLLQKLR AKGIRNPDHS
310 320 330 340 350
RALIKEKLTA DPDSEVATTS LRVSLMCPLG KMRLTVPCRA LTCAHLQSFD
360 370 380 390 400
AALYLQMNEK KPTWTCPVCD KKAPYESLII DGLFMEILSS CSDCDEIQFM
410 420 430 440 450
EDGSWCPMKP KKEASEVCPP PGYGLDGLQY SPVQGGDPSE NKKKVEVIDL
460 470 480 490 500
TIESSSDEED LPPTKKHCSV TSAAIPALPG SKGVLTSGHQ PSSVLRSPAM
510 520 530 540 550
GTLGGDFLSS LPLHEYPPAF PLGADIQGLD LFSFLQTESQ HYGPSVITSL
560 570 580 590 600
DEQDALGHFF QYRGTPSHFL GPLAPTLGSS HCSATPAPPP GRVSSIVAPG
610 620
GALREGHGGP LPSGPSLTGC RSDIISLD
Length:628
Mass (Da):68,017
Last modified:December 7, 2004 - v2
Checksum:i7C06EF599D48F87D
GO

Sequence cautioni

The sequence BAA78533.1 differs from that shown. Reason: Erroneous initiation.

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti390 – 3901S → C.
Corresponds to variant rs17354559 [ dbSNP | Ensembl ].
VAR_050535

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB021868 mRNA. Translation: BAA78533.1. Different initiation.
BC001154 mRNA. Translation: AAH01154.2.
BC030556 mRNA. Translation: AAH30556.2.
CCDSiCCDS72866.1.
PIRiT34525.
RefSeqiNP_006090.2. NM_006099.3.
UniGeneiHs.435761.

Genome annotation databases

EnsembliENST00000393045; ENSP00000376765; ENSG00000131788.
GeneIDi10401.
KEGGihsa:10401.
UCSCiuc001eoc.1. human.

Polymorphism databases

DMDMi56405390.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB021868 mRNA. Translation: BAA78533.1 . Different initiation.
BC001154 mRNA. Translation: AAH01154.2 .
BC030556 mRNA. Translation: AAH30556.2 .
CCDSi CCDS72866.1.
PIRi T34525.
RefSeqi NP_006090.2. NM_006099.3.
UniGenei Hs.435761.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4MVT X-ray 2.30 A/B/C/D 112-467 [» ]
ProteinModelPortali Q9Y6X2.
SMRi Q9Y6X2. Positions 1-65, 126-408.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 115673. 53 interactions.
DIPi DIP-5969N.
IntActi Q9Y6X2. 25 interactions.
MINTi MINT-149343.
STRINGi 9606.ENSP00000376765.

PTM databases

PhosphoSitei Q9Y6X2.

Polymorphism databases

DMDMi 56405390.

Proteomic databases

MaxQBi Q9Y6X2.
PaxDbi Q9Y6X2.
PRIDEi Q9Y6X2.

Protocols and materials databases

DNASUi 10401.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000393045 ; ENSP00000376765 ; ENSG00000131788 .
GeneIDi 10401.
KEGGi hsa:10401.
UCSCi uc001eoc.1. human.

Organism-specific databases

CTDi 10401.
GeneCardsi GC01P145575.
HGNCi HGNC:16861. PIAS3.
MIMi 605987. gene.
neXtProti NX_Q9Y6X2.
PharmGKBi PA134989011.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG125513.
GeneTreei ENSGT00550000074410.
HOGENOMi HOG000230594.
HOVERGENi HBG053598.
InParanoidi Q9Y6X2.
KOi K16064.
OMAi KHMVMSF.
PhylomeDBi Q9Y6X2.
TreeFami TF323787.

Enzyme and pathway databases

UniPathwayi UPA00886 .
SignaLinki Q9Y6X2.

Miscellaneous databases

ChiTaRSi PIAS3. human.
GeneWikii PIAS3.
GenomeRNAii 10401.
NextBioi 39416.
PROi Q9Y6X2.
SOURCEi Search...

Gene expression databases

Bgeei Q9Y6X2.
CleanExi HS_PIAS3.
ExpressionAtlasi Q9Y6X2. baseline and differential.
Genevestigatori Q9Y6X2.

Family and domain databases

Gene3Di 1.10.720.30. 1 hit.
InterProi IPR027226. PIAS3.
IPR023321. PINIT.
IPR003034. SAP_dom.
IPR004181. Znf_MIZ.
[Graphical view ]
PANTHERi PTHR10782:SF10. PTHR10782:SF10. 1 hit.
Pfami PF14324. PINIT. 1 hit.
PF02891. zf-MIZ. 1 hit.
[Graphical view ]
SMARTi SM00513. SAP. 1 hit.
[Graphical view ]
PROSITEi PS51466. PINIT. 1 hit.
PS50800. SAP. 1 hit.
PS51044. ZF_SP_RING. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation and chromosomal assignment of a human gene encoding protein inhibitor of activated STAT3 (PIAS3)."
    Ueki N., Seki N., Yano K., Saito T., Masuho Y., Muramatsu M.-A.
    J. Hum. Genet. 44:193-196(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 2-628.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Blood and Muscle.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 310-628.
    Tissue: Mammary cancer.
  4. "Specific inhibition of Stat3 signal transduction by PIAS3."
    Chung C.D., Liao J., Liu B., Rao X., Jay P., Berta P., Shuai K.
    Science 278:1803-1805(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH STAT3, TISSUE SPECIFICITY.
  5. "Protein inhibitor of activated STAT3 regulates androgen receptor signaling in prostate carcinoma cells."
    Junicho A., Matsuda T., Yamamoto T., Kishi H., Korkmaz K., Saatcioglu F., Fuse H., Muraguchi A.
    Biochem. Biophys. Res. Commun. 278:9-13(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH AR, INDUCTION.
  6. "The zinc finger protein Gfi-1 can enhance STAT3 signaling by interacting with the STAT3 inhibitor PIAS3."
    Roedel B., Tavassoli K., Karsunky H., Schmidt T., Bachmann M., Schaper F., Heinrich P., Shuai K., Elsaesser H.-P., Moeroey T.
    EMBO J. 19:5845-5855(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH GFI1.
  7. "ATBF1 enhances the suppression of STAT3 signaling by interaction with PIAS3."
    Nojiri S., Joh T., Miura Y., Sakata N., Nomura T., Nakao H., Sobue S., Ohara H., Asai K., Ito M.
    Biochem. Biophys. Res. Commun. 314:97-103(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ZFHX3.
  8. "Repression of the transactivating capacity of the oncoprotein PLAG1 by SUMOylation."
    Van Dyck F., Delvaux E.L.D., Van de Ven W.J.M., Chavez M.V.
    J. Biol. Chem. 279:36121-36131(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PLAG1.
  9. "SUMOylation and SUMO-interacting motif (SIM) of metastasis tumor antigen 1 (MTA1) synergistically regulate its transcriptional repressor function."
    Cong L., Pakala S.B., Ohshiro K., Li D.Q., Kumar R.
    J. Biol. Chem. 286:43793-43808(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH MTA1.

Entry informationi

Entry nameiPIAS3_HUMAN
AccessioniPrimary (citable) accession number: Q9Y6X2
Secondary accession number(s): Q9UFI3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 5, 2002
Last sequence update: December 7, 2004
Last modified: October 29, 2014
This is version 132 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  7. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3