Reviewed, UniProtKB/Swiss-Prot Q9Y6W6 (DUS10_HUMAN)

Names and origin

Protein names

Recommended name:
    Dual specificity protein phosphatase 10
    EC=3.1.3.48
    EC=3.1.3.16
Alternative name(s):
    Mitogen-activated protein kinase phosphatase 5
      Short name=MAP kinase phosphatase 5
      Short name=MKP-5

Gene names

Name:	DUSP10
Synonyms:	MKP5

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	482 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in the inactivation of MAP kinases. Has a specificity for the MAPK11/MAPK12/MAPK13/MAPK14 subfamily.
Catalytic activity	Protein tyrosine phosphate + H2O = protein tyrosine + phosphate. A phosphoprotein + H2O = a protein + phosphate.
Subcellular location	Cytoplasm. Nucleus.
Sequence similarities	Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily. Contains 1 rhodanese domain. Contains 1 tyrosine-protein phosphatase domain.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Alternative splicing
Molecular function	Hydrolase Protein phosphatase
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	JNK cascade Ref.1 Traceable author statement. Source: ProtInc protein amino acid dephosphorylation Ref.1 Traceable author statement. Source: ProtInc
Cellular component	cytoplasm Ref.1 Traceable author statement. Source: ProtInc nucleus Ref.1 Traceable author statement. Source: ProtInc
Molecular function	MAP kinase tyrosine/serine/threonine phosphatase activity Inferred from electronic annotation. Source: InterPro protein tyrosine phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9Y6W6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9Y6W6-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-342: Missing.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 482

482

Dual specificity protein phosphatase 10

PRO_0000094813

Regions

Domain

168 – 285

118

Rhodanese

Domain

384 – 453

70

Tyrosine-protein phosphatase

Sites

Active site

408

1

Phosphocysteine intermediate By similarity

Amino acid modifications

Modified residue

4

1

Phosphoserine Ref.7

Natural variations

Alternative sequence

1 – 342

342

Missing in isoform 2.

VSP_036549

Secondary structure

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482

Helix Strand Turn

Details...

Helix

152 – 160

9

Beta strand

173 – 176

4

Helix

180 – 185

6

Beta strand

186 – 188

3

Helix

199 – 206

8

Helix

212 – 217

6

Helix

224 – 230

7

Beta strand

233 – 236

4

Helix

243 – 245

3

Helix

251 – 261

11

Beta strand

267 – 269

3

Helix

272 – 276

5

Turn

277 – 279

3

Helix

281 – 283

3

Beta strand

284 – 286

3

Beta strand

323 – 326

4

Beta strand

329 – 332

4

Helix

336 – 338

3

Helix

340 – 345

6

Beta strand

348 – 353

6

Beta strand

356 – 358

3

Helix

363 – 365

3

Beta strand

368 – 372

5

Beta strand

378 – 380

3

Helix

384 – 386

3

Helix

387 – 399

13

Beta strand

403 – 407

5

Beta strand

409 – 413

5

Helix

414 – 426

13

Helix

431 – 441

11

Helix

449 – 463

15

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: A8CB74ABF9498CD4	FASTA	482	52,642
	10 20 30 40 50 60 MPPSPLDDRV VVALSRPVRP QDLNLCLDSS YLGSANPGSN SHPPVIATTV VSLKAANLTY 70 80 90 100 110 120 MPSSSGSARS LNCGCSSASC CTVATYDKDN QAQTQAIAAG TTTTAIGTST TCPANQMVNN 130 140 150 160 170 180 NENTGSLSPS SGVGSPVSGT PKQLASIKII YPNDLAKKMT KCSKSHLPSQ GPVIIDCRPF 190 200 210 220 230 240 MEYNKSHIQG AVHINCADKI SRRRLQQGKI TVLDLISCRE GKDSFKRIFS KEIIVYDENT 250 260 270 280 290 300 NEPSRVMPSQ PLHIVLESLK REGKEPLVLK GGLSSFKQNH ENLCDNSLQL QECREVGGGA 310 320 330 340 350 360 SAASSLLPQP IPTTPDIENA ELTPILPFLF LGNEQDAQDL DTMQRLNIGY VINVTTHLPL 370 380 390 400 410 420 YHYEKGLFNY KRLPATDSNK QNLRQYFEEA FEFIEEAHQC GKGLLIHCQA GVSRSATIVI 430 440 450 460 470 480 AYLMKHTRMT MTDAYKFVKG KRPIISPNLN FMGQLLEFEE DLNNGVTPRI LTPKLMGVET VV
	Isoform 2. Checksum: CE151F365D042516	FASTA	140	16,110
	10 20 30 40 50 60 MQRLNIGYVI NVTTHLPLYH YEKGLFNYKR LPATDSNKQN LRQYFEEAFE FIEEAHQCGK 70 80 90 100 110 120 GLLIHCQAGV SRSATIVIAY LMKHTRMTMT DAYKFVKGKR PIISPNLNFM GQLLEFEEDL 130 140 NNGVTPRILT PKLMGVETVV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and characterization of a novel dual specificity phosphatase, MKP-5." Tanoue T., Moriguchi T., Nishida E. J. Biol. Chem. 274:19949-19956(1999) [PubMed: 10391943] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"MKP5, a new member of the MAP kinase phosphatase family, which selectively dephosphorylates stress-activated kinases." Theodosiou A., Smith A., Gillieron C., Arkinstall S., Ashworth A. Oncogene 18:6981-6988(1999) [PubMed: 10597297] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[4]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed: 16710414] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Brain, Lung, PNS and Testis.
[7]	"Phosphoproteome analysis of the human mitotic spindle." Nousiainen M., Sillje H.H.W., Sauer G., Nigg E.A., Koerner R. Proc. Natl. Acad. Sci. U.S.A. 103:5391-5396(2006) [PubMed: 16565220] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-4, MASS SPECTROMETRY. Tissue: Epithelium.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AB026436 mRNA. Translation: BAA81668.1.
AF179212 mRNA. Translation: AAD51857.1.
AK022513 mRNA. Translation: BAB14070.1.
AL590966 Genomic DNA. Translation: CAH71120.1.
CH471100 Genomic DNA. Translation: EAW93283.1.
BC020608 mRNA. Translation: AAH20608.1.
BC031405 mRNA. Translation: AAH31405.1.
BC063826 mRNA. Translation: AAH63826.1.

IPI

IPI00026987.
IPI00939755.

RefSeq

NP_009138.1.
NP_653329.1.
NP_653330.1.

UniGene

Hs.497822

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1ZZW	X-ray	1.60	A/B	320-467	[»]
2OUC	X-ray	2.20	A/B	148-287	[»]
2OUD	X-ray	2.80	A	315-482	[»]

ModBase

Search...

Protein-protein interaction databases

STRING

Q9Y6W6.

PTM databases

PhosphoSite

Q9Y6W6.

Proteomic databases

PRIDE

Q9Y6W6.

Genome annotation databases

Ensembl

ENST00000323825; ENSP00000322015; ENSG00000143507; Homo sapiens. [Genome view]
ENST00000366899; ENSP00000355866; ENSG00000143507; Homo sapiens. [Genome view]
ENST00000391885; ENSP00000375757; ENSG00000143507; Homo sapiens. [Genome view]
ENST00000391886; ENSP00000375758; ENSG00000143507; Homo sapiens. [Genome view]
ENST00000418487; ENSP00000390238; ENSG00000143507; Homo sapiens. [Genome view]

GeneID

11221.

KEGG

hsa:11221.

UCSC

uc001hmx.1. human.
uc001hmy.1. human.

Organism-specific databases

CTD

11221.

GeneCards

GC01M219941.

H-InvDB

HIX0001610.

HGNC

HGNC:3065. DUSP10.

HPA

HPA016758.

MIM

608867. gene.

PharmGKB

PA27520.

GenAtlas

Search...

Phylogenomic databases

HOGENOM

Q9Y6W6.

HOVERGEN

Q9Y6W6.

OMA

NEHDAQD.

Enzyme and pathway databases

BRENDA

3.1.3.16. 247.
3.1.3.48. 247.

Pathway_Interaction_DB

p38alphabetapathway. Regulation of p38-alpha and p38-beta.

Gene expression databases

ArrayExpress

Q9Y6W6.

Bgee

Q9Y6W6.

CleanEx

HS_DUSP10.

Genevestigator

Q9Y6W6.

GermOnline

ENSG00000143507. Homo sapiens.

Family and domain databases

InterPro

IPR000387. Dual-sp/Tyr_phosphatase.
IPR020417. Dual-sp_phosphatase.
IPR000340. Dual-sp_phosphatase_cat-dom.
IPR020422. Dual-sp_phosphatase_subgr_cat.
IPR008343. MAPK_phosph.
IPR001763. Rhodanese-like.
IPR016130. Tyr_Pase_AS.
[Graphical view]

Gene3D

G3DSA:3.40.250.10. Rhodanese-like. 1 hit.

Pfam

PF00782. DSPc. 1 hit.
PF00581. Rhodanese. 1 hit.
[Graphical view]

PRINTS

PR01908. ADSPHPHTASE.
PR01764. MAPKPHPHTASE.

SMART

SM00195. DSPc. 1 hit.
SM00450. RHOD. 1 hit.
[Graphical view]

PROSITE

PS50206. RHODANESE_3. 1 hit.
PS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

42707.

SOURCE

Search...

Entry information

Entry name

DUS10_HUMAN

Accession

Primary (citable) accession number: Q9Y6W6
Secondary accession number(s): Q6GSI4, Q9H9Z5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2002
Last sequence update:	November 1, 1999
Last modified:	November 3, 2009
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.