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Protein

Wiskott-Aldrich syndrome protein family member 2

Gene

WASF2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Downstream effector molecule involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. Promotes formation of actin filaments. Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex.2 Publications

GO - Molecular functioni

  • actin binding Source: ProtInc
  • cadherin binding Source: BHF-UCL
  • protein complex binding Source: UniProtKB
  • SH3 domain binding Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionActin-binding
Biological processHost-virus interaction

Enzyme and pathway databases

ReactomeiR-HSA-2029482. Regulation of actin dynamics for phagocytic cup formation.
R-HSA-4420097. VEGFA-VEGFR2 Pathway.
R-HSA-5663213. RHO GTPases Activate WASPs and WAVEs.
SignaLinkiQ9Y6W5.
SIGNORiQ9Y6W5.

Names & Taxonomyi

Protein namesi
Recommended name:
Wiskott-Aldrich syndrome protein family member 2
Short name:
WASP family protein member 2
Alternative name(s):
Protein WAVE-2
Verprolin homology domain-containing protein 2
Gene namesi
Name:WASF2Imported
Synonyms:WAVE2Imported
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiHostDB:ENSG00000158195.10.
HGNCiHGNC:12733. WASF2.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell projection, Cytoplasm, Cytoskeleton

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi40L → D: Decreased interaction with WAVE complex; when associated with D-51. 1 Publication1
Mutagenesisi50I → D: Decreased interaction with WAVE complex; when associated with D-54. 1 Publication1
Mutagenesisi51F → D: Decreased interaction with WAVE complex; when associated with D-40. 1 Publication1
Mutagenesisi54L → D: Decreased interaction with WAVE complex; when associated with D-50. 1 Publication1
Mutagenesisi124Y → D: Constitutive induction of the formation of actin filaments. Strongly enhances formation of lamellipodia. 1 Publication1
Mutagenesisi150Y → D: Constitutive induction of the formation of actin filaments. Strongly enhances formation of lamellipodia. 1 Publication1
Mutagenesisi160 – 161WK → ED: Constitutive induction of the formation of actin filaments. Strongly enhances formation of lamellipodia. 1 Publication2

Organism-specific databases

DisGeNETi10163.
OpenTargetsiENSG00000158195.
PharmGKBiPA37344.

Polymorphism and mutation databases

BioMutaiWASF2.
DMDMi59800456.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001889941 – 498Wiskott-Aldrich syndrome protein family member 2Add BLAST498

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei474PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9Y6W5.
PaxDbiQ9Y6W5.
PeptideAtlasiQ9Y6W5.
PRIDEiQ9Y6W5.

PTM databases

iPTMnetiQ9Y6W5.
PhosphoSitePlusiQ9Y6W5.

Miscellaneous databases

PMAP-CutDBiQ9Y6W5.

Expressioni

Tissue specificityi

Expressed in all tissues with strongest expression in placenta, lung, and peripheral blood leukocytes, but not in skeletal muscle.1 Publication

Gene expression databases

BgeeiENSG00000158195.
CleanExiHS_WASF2.
GenevisibleiQ9Y6W5. HS.

Organism-specific databases

HPAiCAB022632.
HPA045288.

Interactioni

Subunit structurei

Binds actin and the Arp2/3 complex. Interacts with BAIAP2. Component of the WAVE2 complex composed of ABI1, CYFIP1/SRA1, NCKAP1/NAP1 (NCKAP1l/HEM1 in hematopoietic cells) and WASF2/WAVE2 (PubMed:16417406). Directly interacts with BRK1. Interacts with human cytomegalovirus protein UL135. Interacts with FNBP1L (via the SH3 domain) (PubMed:19798448).8 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • actin binding Source: ProtInc
  • cadherin binding Source: BHF-UCL
  • protein complex binding Source: UniProtKB
  • SH3 domain binding Source: UniProtKB

Protein-protein interaction databases

BioGridi115465. 38 interactors.
CORUMiQ9Y6W5.
DIPiDIP-41817N.
ELMiQ9Y6W5.
IntActiQ9Y6W5. 15 interactors.
MINTiMINT-5005787.
STRINGi9606.ENSP00000396211.

Structurei

Secondary structure

1498
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi436 – 446Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2A40X-ray1.80C/F433-464[»]
ProteinModelPortaliQ9Y6W5.
SMRiQ9Y6W5.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9Y6W5.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini436 – 453WH2PROSITE-ProRule annotationAdd BLAST18

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi268 – 271Poly-Pro4
Compositional biasi318 – 328Poly-ProAdd BLAST11
Compositional biasi333 – 338Poly-Pro6
Compositional biasi347 – 350Poly-Pro4
Compositional biasi364 – 371Poly-Pro8
Compositional biasi379 – 382Poly-Pro4
Compositional biasi391 – 406Poly-ProAdd BLAST16

Domaini

Binds and activates the Arp2/3 complex via the C-terminal domain. Interacts with actin via the WH2 domain.2 Publications

Sequence similaritiesi

Belongs to the SCAR/WAVE family.Curated

Phylogenomic databases

eggNOGiKOG1830. Eukaryota.
ENOG410Y6V4. LUCA.
GeneTreeiENSGT00730000110593.
HOGENOMiHOG000021456.
HOVERGENiHBG058482.
InParanoidiQ9Y6W5.
KOiK05748.
OMAiSYPADNQ.
OrthoDBiEOG091G08O1.
PhylomeDBiQ9Y6W5.
TreeFamiTF315031.

Family and domain databases

InterProiView protein in InterPro
IPR028288. SCAR/WAVE_fam.
IPR003124. WH2_dom.
PANTHERiPTHR12902. PTHR12902. 1 hit.
PfamiView protein in Pfam
PF02205. WH2. 1 hit.
SMARTiView protein in SMART
SM00246. WH2. 1 hit.
PROSITEiView protein in PROSITE
PS51082. WH2. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9Y6W5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPLVTRNIEP RHLCRQTLPS VRSELECVTN ITLANVIRQL GSLSKYAEDI
60 70 80 90 100
FGELFTQANT FASRVSSLAE RVDRLQVKVT QLDPKEEEVS LQGINTRKAF
110 120 130 140 150
RSSTIQDQKL FDRNSLPVPV LETYNTCDTP PPLNNLTPYR DDGKEALKFY
160 170 180 190 200
TDPSYFFDLW KEKMLQDTKD IMKEKRKHRK EKKDNPNRGN VNPRKIKTRK
210 220 230 240 250
EEWEKMKMGQ EFVESKEKLG TSGYPPTLVY QNGSIGCVEN VDASSYPPPP
260 270 280 290 300
QSDSASSPSP SFSEDNLPPP PAEFSYPVDN QRGSGLAGPK RSSVVSPSHP
310 320 330 340 350
PPAPPLGSPP GPKPGFAPPP APPPPPPPMI GIPPPPPPVG FGSPGTPPPP
360 370 380 390 400
SPPSFPPHPD FAAPPPPPPP PAADYPTLPP PPLSQPTGGA PPPPPPPPPP
410 420 430 440 450
GPPPPPFTGA DGQPAIPPPL SDTTKPKSSL PAVSDARSDL LSAIRQGFQL
460 470 480 490
RRVEEQREQE KRDVVGNDVA TILSRRIAVE YSDSEDDSSE FDEDDWSD
Length:498
Mass (Da):54,284
Last modified:February 1, 2005 - v3
Checksum:iC737CE963016DE94
GO
Isoform 2 (identifier: Q9Y6W5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     275-281: SYPVDNQ → RFSAAQG
     282-498: Missing.

Note: No experimental confirmation available.
Show »
Length:281
Mass (Da):31,979
Checksum:iC5E79110854FAF56
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_042514275 – 281SYPVDNQ → RFSAAQG in isoform 2. 1 Publication7
Alternative sequenceiVSP_042515282 – 498Missing in isoform 2. 1 PublicationAdd BLAST217

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB026542 mRNA. Translation: BAA81795.1.
AK303011 mRNA. Translation: BAG64142.1.
AL096774 Genomic DNA. Translation: CAC18518.1.
AL663122 Genomic DNA. No translation available.
BX293535 Genomic DNA. No translation available.
BC040943 mRNA. Translation: AAH40943.1.
CCDSiCCDS304.1. [Q9Y6W5-1]
CCDS55582.1. [Q9Y6W5-2]
RefSeqiNP_001188333.1. NM_001201404.2. [Q9Y6W5-2]
NP_008921.1. NM_006990.4. [Q9Y6W5-1]
UniGeneiHs.469244.
Hs.512079.

Genome annotation databases

EnsembliENST00000536657; ENSP00000439883; ENSG00000158195. [Q9Y6W5-2]
ENST00000618852; ENSP00000483313; ENSG00000158195. [Q9Y6W5-1]
GeneIDi10163.
KEGGihsa:10163.
UCSCiuc057dvq.1. human. [Q9Y6W5-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiWASF2_HUMAN
AccessioniPrimary (citable) accession number: Q9Y6W5
Secondary accession number(s): B4DZN0, O60794, Q9UDY7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: February 1, 2005
Last modified: September 27, 2017
This is version 160 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families