Q9Y6W5 (WASF2_HUMAN) Reviewed, UniProtKB/Swiss-Prot
Last modified
December 14, 2011.
Version 102.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Wiskott-Aldrich syndrome protein family member 2 Short name=WASP family protein member 2 Alternative name(s): Protein WAVE-2 Verprolin homology domain-containing protein 2 | ||||
| Gene names |
| ||||
| Organism | Homo sapiens (Human) | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 498 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Downstream effector molecule involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. Promotes formation of actin filaments. Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex. Ref.1 Ref.9 |
| Subunit structure | Binds actin and the Arp2/3 complex. Interacts with BAIAP2. Component of the WAVE2 complex composed of ABI1, CYFIP1/SRA1, NCKAP1/NAP1 and WASF2/WAVE2. Directly interacts with BRK1. Ref.1 Ref.4 Ref.5 Ref.7 Ref.9 |
| Subcellular location | Cytoplasm › cytoskeleton By similarity. Cell projection › lamellipodium By similarity. Note: At the interface between the lamellipodial actin meshwork and the membrane By similarity. |
| Tissue specificity | Expressed in all tissues with strongest expression in placenta, lung, and peripheral blood leukocytes, but not in skeletal muscle. Ref.1 |
| Domain | Binds and activates the Arp2/3 complex via the C-terminal domain. Interacts with actin via the WH2 domain. Ref.1 Ref.9 |
| Sequence similarities | Belongs to the SCAR/WAVE family. Contains 1 WH2 domain. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Cell projection Cytoplasm Cytoskeleton |
| Ligand | Actin-binding |
| PTM | Phosphoprotein |
| Technical term | 3D-structure Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Biological process | G-protein signaling, coupled to cAMP nucleotide second messenger Traceable author statement. Source: ProtInc actin cytoskeleton organizationTraceable author statement. Source: ProtInc |
| Cellular component | actin cytoskeleton Traceable author statement. Source: ProtInc lamellipodiumInferred from direct assay. Source: MGI |
| Molecular function | actin binding Traceable author statement. Source: ProtInc |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||||
Molecule processing | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 498 | 498 | Wiskott-Aldrich syndrome protein family member 2 | PRO_0000188994 | |||||||
Regions | |||||||||||
| Domain | 436 – 453 | 18 | WH2 | ||||||||
| Compositional bias | 268 – 271 | 4 | Poly-Pro | ||||||||
| Compositional bias | 318 – 328 | 11 | Poly-Pro | ||||||||
| Compositional bias | 333 – 338 | 6 | Poly-Pro | ||||||||
| Compositional bias | 347 – 350 | 4 | Poly-Pro | ||||||||
| Compositional bias | 364 – 371 | 8 | Poly-Pro | ||||||||
| Compositional bias | 379 – 382 | 4 | Poly-Pro | ||||||||
| Compositional bias | 391 – 406 | 16 | Poly-Pro | ||||||||
Amino acid modifications | |||||||||||
| Modified residue | 482 | 1 | Phosphoserine Ref.6 | ||||||||
| Modified residue | 484 | 1 | Phosphoserine Ref.6 | ||||||||
| Modified residue | 488 | 1 | Phosphoserine Ref.6 | ||||||||
| Modified residue | 489 | 1 | Phosphoserine Ref.6 | ||||||||
Experimental info | |||||||||||
| Mutagenesis | 40 | 1 | L → D: Decreased interaction with WAVE complex; when associated with D-51. Ref.7 | ||||||||
| Mutagenesis | 50 | 1 | I → D: Decreased interaction with WAVE complex; when associated with D-54. Ref.7 | ||||||||
| Mutagenesis | 51 | 1 | F → D: Decreased interaction with WAVE complex; when associated with D-40. Ref.7 | ||||||||
| Mutagenesis | 54 | 1 | L → D: Decreased interaction with WAVE complex; when associated with D-50. Ref.7 | ||||||||
| Mutagenesis | 124 | 1 | Y → D: Constitutive induction of the formation of actin filaments. Strongly enhances formation of lamellipodia. Ref.9 | ||||||||
| Mutagenesis | 150 | 1 | Y → D: Constitutive induction of the formation of actin filaments. Strongly enhances formation of lamellipodia. Ref.9 | ||||||||
| Mutagenesis | 160 – 161 | 2 | WK → ED: Constitutive induction of the formation of actin filaments. Strongly enhances formation of lamellipodia. | ||||||||
Secondary structure | |||||||||||
Helix Strand Turn | |||||||||||
| Helix | 436 – 446 | 11 | |||||||||
Sequences
| ||||||||||||||||||
References
| « Hide 'large scale' references | |
| [1] | "Identification of two human WAVE/SCAR homologues as general actin regulatory molecules which associate with the Arp2/3 complex." Suetsugu S., Miki H., Takenawa T. Biochem. Biophys. Res. Commun. 260:296-302(1999) [PubMed: 10381382] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH ACTIN AND THE ARP2/3 COMPLEX, SUBUNIT, DOMAIN, TISSUE SPECIFICITY. Tissue: T-cell lymphoma. |
| [2] | "The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. Bentley D.R.Nature 441:315-321(2006) [PubMed: 16710414] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [3] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lymph. |
| [4] | "IRSp53 is an essential intermediate between Rac and WAVE in the regulation of membrane ruffling." Miki H., Yamaguchi H., Suetsugu S., Takenawa T. Nature 408:732-735(2000) [PubMed: 11130076] [Abstract] Cited for: INTERACTION WITH BAIAP2. |
| [5] | "Purification and architecture of the ubiquitous Wave complex." Gautreau A., Ho H.-Y., Li J., Steen H., Gygi S.P., Kirschner M.W. Proc. Natl. Acad. Sci. U.S.A. 101:4379-4383(2004) [PubMed: 15070726] [Abstract] Cited for: INTERACTION WITH C3ORF10. |
| [6] | "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-482; SER-484; SER-488 AND SER-489, MASS SPECTROMETRY. Tissue: Cervix carcinoma. |
| [7] | "Structure and control of the actin regulatory WAVE complex." Chen Z., Borek D., Padrick S.B., Gomez T.S., Metlagel Z., Ismail A.M., Umetani J., Billadeau D.D., Otwinowski Z., Rosen M.K. Nature 468:533-538(2010) [PubMed: 21107423] [Abstract] Cited for: MUTAGENESIS OF LEU-40; ILE-50; PHE-51 AND LEU-54, SUBUNIT. |
| [8] | "Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. |
| [9] | "Actin-bound structures of Wiskott-Aldrich syndrome protein (WASP)-homology domain 2 and the implications for filament assembly." Chereau D., Kerff F., Graceffa P., Grabarek Z., Langsetmo K., Dominguez R. Proc. Natl. Acad. Sci. U.S.A. 102:16644-16649(2005) [PubMed: 16275905] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 433-464 IN COMPLEX WITH ACTIN, FUNCTION, DOMAIN, MUTAGENESIS OF TYR-124; TYR-150 AND 160-TRP-LYS-161, SUBUNIT. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| EMBL GenBank DDBJ | AB026542 mRNA. Translation: BAA81795.1. AL096774 Genomic DNA. Translation: CAC18518.1. BC040943 mRNA. Translation: AAH40943.1. | ||||||||||||
| IPI | IPI00472164. | ||||||||||||
| RefSeq | NP_008921.1. NM_006990.3. | ||||||||||||
| UniGene | Hs.469244. Hs.590909. | ||||||||||||
3D structure databases | |||||||||||||
| PDBe RCSB PDB PDBj |
| ||||||||||||
| ProteinModelPortal | Q9Y6W5. | ||||||||||||
| SMR | Q9Y6W5. Positions 20-206, 435-482. | ||||||||||||
| ModBase | Search... | ||||||||||||
Protein-protein interaction databases | |||||||||||||
| MINT | MINT-5005787. | ||||||||||||
| STRING | Q9Y6W5. | ||||||||||||
PTM databases | |||||||||||||
| PhosphoSite | Q9Y6W5. | ||||||||||||
Polymorphism databases | |||||||||||||
| DMDM | 59800456. | ||||||||||||
Proteomic databases | |||||||||||||
| PeptideAtlas | Q9Y6W5. | ||||||||||||
| PRIDE | Q9Y6W5. | ||||||||||||
Protocols and materials databases | |||||||||||||
| StructuralBiologyKnowledgebase | Search... | ||||||||||||
Genome annotation databases | |||||||||||||
| Ensembl | ENST00000430629; ENSP00000396211; ENSG00000158195. | ||||||||||||
| GeneID | 10163. | ||||||||||||
| KEGG | hsa:10163. | ||||||||||||
| UCSC | uc001bof.1. human. | ||||||||||||
Organism-specific databases | |||||||||||||
| CTD | 10163. | ||||||||||||
| GeneCards | GC01M027730. | ||||||||||||
| H-InvDB | HIX0000310. | ||||||||||||
| HGNC | HGNC:12733. WASF2. | ||||||||||||
| HPA | CAB022632. | ||||||||||||
| MIM | 605875. gene. | ||||||||||||
| neXtProt | NX_Q9Y6W5. | ||||||||||||
| GenAtlas | Search... | ||||||||||||
Phylogenomic databases | |||||||||||||
| eggNOG | prNOG06683. | ||||||||||||
| GeneTree | ENSGT00550000074443. | ||||||||||||
| HOVERGEN | HBG058482. | ||||||||||||
| InParanoid | Q9Y6W5. | ||||||||||||
| OMA | IGSNESM. | ||||||||||||
| OrthoDB | EOG49W2GB. | ||||||||||||
| PhylomeDB | Q9Y6W5. | ||||||||||||
Gene expression databases | |||||||||||||
| ArrayExpress | Q9Y6W5. | ||||||||||||
| Bgee | Q9Y6W5. | ||||||||||||
| CleanEx | HS_WASF2. | ||||||||||||
| Genevestigator | Q9Y6W5. | ||||||||||||
| GermOnline | ENSG00000158195. Homo sapiens. | ||||||||||||
Family and domain databases | |||||||||||||
| InterPro | IPR003124. WH2_actin-bd. [Graphical view] | ||||||||||||
| KO | K05748. | ||||||||||||
| Pfam | PF02205. WH2. 1 hit. [Graphical view] | ||||||||||||
| SMART | SM00246. WH2. 1 hit. [Graphical view] | ||||||||||||
| PROSITE | PS51082. WH2. 1 hit. [Graphical view] | ||||||||||||
| ProtoNet | Search... | ||||||||||||
Other | |||||||||||||
| NextBio | 38478. | ||||||||||||
| PMAP-CutDB | Q9Y6W5. | ||||||||||||
| SOURCE | Search... | ||||||||||||
Entry information
| Entry name | WASF2_HUMAN | ||||||||
| Accession | Primary (citable) accession number: Q9Y6W5 Secondary accession number(s): O60794, Q9UDY7 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Chordata Protein Annotation Program | ||||||||
| Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. | ||||||||
Relevant documents
| Human chromosome 1 Human chromosome 1: entries, gene names and cross-references to MIM |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| PDB cross-references Index of Protein Data Bank (PDB) cross-references |
| SIMILARITY comments Index of protein domains and families |

Clusters with