Q9Y6W3 (CAN7_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Calpain-7
EC=3.4.22.-

Alternative name(s):
PalB homolog
Short name=PalBH

Gene names

Name:	CAPN7
Synonyms:	PALBH

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	813 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Calcium-regulated non-lysosomal thiol-protease By similarity.
Catalytic activity	Broad endopeptidase specificity.
Subcellular location	Nucleus.
Tissue specificity	Ubiquitous.
Sequence similarities	Belongs to the peptidase C2 family. Contains 1 calpain catalytic domain.

Ontologies

Keywords
Cellular component	Nucleus
Domain	Repeat
Molecular function	Hydrolase Protease Thiol protease
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	self proteolysis Inferred from direct assay PubMed 20849418. Source: UniProtKB
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	calcium-dependent cysteine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 813

813

Calpain-7

PRO_0000207720

Regions

Domain

232 – 540

309

Calpain catalytic

Region

541 – 701

161

Domain III

Region

702 – 813

112

Domain N

Sites

Active site

290

1

By similarity

Active site

458

1

By similarity

Active site

578

1

By similarity

Amino acid modifications

Modified residue

95

1

Phosphothreonine Ref.2

Secondary structure

1

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813

Helix Strand Turn

Details...

Beta strand

688 – 696

9

Helix

698 – 700

3

Turn

708 – 710

3

Helix

711 – 713

3

Beta strand

717 – 723

7

Beta strand

725 – 732

8

Beta strand

737 – 747

11

Beta strand

758 – 761

4

Beta strand

766 – 777

12

Beta strand

779 – 790

12

Beta strand

795 – 805

11

Beta strand

808 – 811

4

Sequences

	Sequence		Length	Mass (Da)
	Q9Y6W3 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: 814769611360D281	FASTA	813	92,652
	10 20 30 40 50 60 MDATALERDA VQFARLAVQR DHEGRYSEAV FYYKEAAQAL IYAEMAGSSL ENIQEKITEY 70 80 90 100 110 120 LERVQALHSA VQSKSADPLK SKHQLDLERA HFLVTQAFDE DEKENVEDAI ELYTEAVDLC 130 140 150 160 170 180 LKTSYETADK VLQNKLKQLA RQALDRAEAL SEPLTKPVGK ISSTSVKPKP PPVRAHFPLG 190 200 210 220 230 240 ANPFLERPQS FISPQSCDAQ GQRYTAEEIE VLRTTSKING IEYVPFMNVD LRERFAYPMP 250 260 270 280 290 300 FCDRWGKLPL SPKQKTTFSK WVRPEDLTNN PTMIYTVSSF SIKQTIVSDC SFVASLAISA 310 320 330 340 350 360 AYERRFNKKL ITGIIYPQNK DGEPEYNPCG KYMVKLHLNG VPRKVIIDDQ LPVDHKGELL 370 380 390 400 410 420 CSYSNNKSEL WVSLIEKAYM KVMGGYDFPG SNSNIDLHAL TGWIPERIAM HSDSQTFSKD 430 440 450 460 470 480 NSFRMLYQRF HKGDVLITAS TGMMTEAEGE KWGLVPTHAY AVLDIREFKG LRFIQLKNPW 490 500 510 520 530 540 SHLRWKGRYS ENDVKNWTPE LQKYLNFDPR TAQKIDNGIF WISWDDLCQY YDVIYLSWNP 550 560 570 580 590 600 GLFKESTCIH STWDAKQGPV KDAYSLANNP QYKLEVQCPQ GGAAVWVLLS RHITDKDDFA 610 620 630 640 650 660 NNREFITMVV YKTDGKKVYY PADPPPYIDG IRINSPHYLT KIKLTTPGTH TFTLVVSQYE 670 680 690 700 710 720 KQNTIHYTVR VYSACSFTFS KIPSPYTLSK RINGKWSGQS AGGCGNFQET HKNNPIYQFH 730 740 750 760 770 780 IEKTGPLLIE LRGPRQYSVG FEVVTVSTLG DPGPHGFLRK SSGDYRCGFC YLELENIPSG 790 800 810 IFNIIPSTFL PKQEGPFFLD FNSIIPIKIT QLQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of PalBH, a mammalian homologue of the Aspergillus atypical calpain PalB." Futai E., Kubo T., Sorimachi H., Suzuki K., Maeda T. Biochim. Biophys. Acta 1517:316-319(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-95, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AB028639 mRNA. Translation: BAA78730.1.

IPI

IPI00003765.

RefSeq

NP_055111.1. NM_014296.2.

UniGene

Hs.595234.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2QFE	X-ray	1.45	A	684-813	[»]

ProteinModelPortal

Q9Y6W3.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q9Y6W3. 2 interactions.

STRING

9606.ENSP00000253693.

Protein family/group databases

MEROPS

C02.028.

PTM databases

PhosphoSite

Q9Y6W3.

Polymorphism databases

DMDM

33112239.

Proteomic databases

PaxDb

Q9Y6W3.

PRIDE

Q9Y6W3.

Protocols and materials databases

DNASU

23473.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000253693; ENSP00000253693; ENSG00000131375.

GeneID

23473.

KEGG

hsa:23473.

UCSC

uc003bzn.3. human.

Organism-specific databases

CTD

23473.

GeneCards

GC03P015222.

HGNC

HGNC:1484. CAPN7.

HPA

HPA046617.

MIM

606400. gene.

neXtProt

NX_Q9Y6W3.

PharmGKB

PA26064.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG277123.

HOGENOM

HOG000006940.

HOVERGEN

HBG036832.

InParanoid

Q9Y6W3.

KO

K08576.

OMA

VFYYKEA.

OrthoDB

EOG4HMJ8K.

PhylomeDB

Q9Y6W3.

Gene expression databases

ArrayExpress

Q9Y6W3.

Bgee

Q9Y6W3.

CleanEx

HS_CAPN7.

Genevestigator

Q9Y6W3.

Family and domain databases

InterPro

IPR022684. Calpain_cysteine_protease.
IPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR007330. MIT.
IPR001300. Peptidase_C2_calpain_cat.
[Graphical view]

Pfam

PF01067. Calpain_III. 1 hit.
PF04212. MIT. 2 hits.
PF00648. Peptidase_C2. 1 hit.
[Graphical view]

PRINTS

PR00704. CALPAIN.

SMART

SM00720. calpain_III. 1 hit.
SM00230. CysPc. 1 hit.
SM00745. MIT. 2 hits.
[Graphical view]

SUPFAM

SSF49758. Peptidase_C2. 2 hits.

PROSITE

PS50203. CALPAIN_CAT. 1 hit.
PS00640. THIOL_PROTEASE_ASN. False negative.
PS00139. THIOL_PROTEASE_CYS. False negative.
PS00639. THIOL_PROTEASE_HIS. False negative.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

CAPN7. human.

EvolutionaryTrace

Q9Y6W3.

GenomeRNAi

23473.

NextBio

45805.

SOURCE

Search...

Entry information

Entry name

CAN7_HUMAN

Accession

Primary (citable) accession number: Q9Y6W3

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2003
Last sequence update:	November 1, 1999
Last modified:	May 1, 2013
This is version 100 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.