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Protein

Adseverin

Gene

SCIN

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Ca2+-dependent actin filament-severing protein that has a regulatory function in exocytosis by affecting the organization of the microfilament network underneath the plasma membrane (PubMed:8547642, PubMed:26365202). Severing activity is inhibited by phosphatidylinositol 4,5-bis-phosphate (PIP2) (By similarity). In vitro, also has barbed end capping and nucleating activities in the presence of Ca2+. Required for megakaryocyte differentiation, maturation, polyploidization and apoptosis with the release of platelet-like particles (PubMed:11568009). Plays a role in osteoclastogenesis (OCG) and actin cytoskeletal organization in osteoclasts (By similarity). Regulates chondrocyte proliferation and differentiation (By similarity). Inhibits cell proliferation and tumorigenesis. Signaling is mediated by MAPK, p38 and JNK pathways (PubMed:11568009).By similarity3 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi538Calcium 1Combined sources1
Metal bindingi539Calcium 1Combined sources1
Metal bindingi562Calcium 1Combined sources1
Metal bindingi643Calcium 2; via carbonyl oxygenCombined sources1
Metal bindingi644Calcium 2Combined sources1
Metal bindingi666Calcium 2Combined sources1

GO - Molecular functioni

GO - Biological processi

  • actin filament capping Source: UniProtKB
  • actin filament severing Source: UniProtKB
  • actin nucleation Source: UniProtKB
  • calcium ion regulated exocytosis Source: UniProtKB
  • negative regulation of cell proliferation Source: UniProtKB
  • positive regulation of actin nucleation Source: UniProtKB
  • positive regulation of apoptotic process Source: UniProtKB
  • positive regulation of megakaryocyte differentiation Source: UniProtKB
  • positive regulation of secretion Source: UniProtKB
  • regulation of chondrocyte differentiation Source: UniProtKB
  • sequestering of actin monomers Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Actin capping

Keywords - Ligandi

Actin-binding, Calcium, Metal-binding

Enzyme and pathway databases

BioCyciZFISH:ENSG00000006747-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Adseverin
Alternative name(s):
Scinderin
Gene namesi
Name:SCIN
Synonyms:KIAA1905
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 7

Organism-specific databases

HGNCiHGNC:21695. SCIN.

Subcellular locationi

GO - Cellular componenti

  • brush border Source: Ensembl
  • cell cortex Source: UniProtKB
  • cell junction Source: UniProtKB-KW
  • cell projection Source: UniProtKB-KW
  • cytoplasm Source: UniProtKB
  • extracellular exosome Source: UniProtKB
  • podosome Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell projection, Cytoplasm, Cytoskeleton

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi310M → D: Increases calcium-independent actin-severing activity. 1 Publication1
Mutagenesisi314E → S: Increases calcium-independent actin-severing activity. 1 Publication1
Mutagenesisi455F → D: Loss of actin-binding. 1 Publication1

Organism-specific databases

DisGeNETi85477.
OpenTargetsiENSG00000006747.
PharmGKBiPA134981389.

Polymorphism and mutation databases

BioMutaiSCIN.
DMDMi57015325.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002187441 – 715AdseverinAdd BLAST715

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei102PhosphotyrosineBy similarity1
Modified residuei599PhosphotyrosineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9Y6U3.
PaxDbiQ9Y6U3.
PeptideAtlasiQ9Y6U3.
PRIDEiQ9Y6U3.

PTM databases

iPTMnetiQ9Y6U3.
PhosphoSitePlusiQ9Y6U3.

Expressioni

Tissue specificityi

Expressed in megakaryocytes.1 Publication

Gene expression databases

BgeeiENSG00000006747.
CleanExiHS_SCIN.
ExpressionAtlasiQ9Y6U3. baseline and differential.
GenevisibleiQ9Y6U3. HS.

Organism-specific databases

HPAiHPA020518.
HPA024264.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi124552. 11 interactors.
IntActiQ9Y6U3. 1 interactor.
MINTiMINT-1141238.
STRINGi9606.ENSP00000297029.

Structurei

Secondary structure

1715
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi8 – 11Combined sources4
Turni12 – 14Combined sources3
Beta strandi15 – 24Combined sources10
Beta strandi29 – 31Combined sources3
Helixi34 – 36Combined sources3
Beta strandi39 – 41Combined sources3
Beta strandi45 – 53Combined sources9
Beta strandi58 – 66Combined sources9
Helixi72 – 88Combined sources17
Turni89 – 91Combined sources3
Beta strandi93 – 99Combined sources7
Turni100 – 102Combined sources3
Helixi105 – 108Combined sources4
Beta strandi115 – 119Combined sources5
Turni122 – 125Combined sources4
Beta strandi138 – 143Combined sources6
Beta strandi145 – 147Combined sources3
Beta strandi149 – 153Combined sources5
Beta strandi164 – 169Combined sources6
Beta strandi171 – 178Combined sources8
Helixi184 – 202Combined sources19
Beta strandi206 – 212Combined sources7
Helixi218 – 223Combined sources6
Beta strandi250 – 255Combined sources6
Beta strandi262 – 272Combined sources11
Helixi274 – 276Combined sources3
Beta strandi281 – 287Combined sources7
Helixi288 – 290Combined sources3
Beta strandi292 – 297Combined sources6
Helixi303 – 319Combined sources17
Beta strandi327 – 332Combined sources6
Helixi338 – 341Combined sources4
Beta strandi344 – 346Combined sources3
Beta strandi397 – 403Combined sources7
Beta strandi405 – 410Combined sources6
Helixi413 – 415Combined sources3
Beta strandi424 – 430Combined sources7
Beta strandi435 – 441Combined sources7
Helixi447 – 463Combined sources17
Beta strandi469 – 474Combined sources6
Helixi480 – 483Combined sources4
Beta strandi491 – 493Combined sources3
Beta strandi511 – 516Combined sources6
Beta strandi519 – 521Combined sources3
Beta strandi524 – 528Combined sources5
Helixi532 – 534Combined sources3
Beta strandi539 – 544Combined sources6
Beta strandi548 – 554Combined sources7
Helixi560 – 572Combined sources13
Beta strandi576 – 581Combined sources6
Helixi587 – 592Combined sources6
Turni603 – 605Combined sources3
Beta strandi615 – 620Combined sources6
Beta strandi627 – 630Combined sources4
Helixi637 – 639Combined sources3
Beta strandi644 – 649Combined sources6
Beta strandi654 – 658Combined sources5
Helixi664 – 674Combined sources11
Beta strandi680 – 683Combined sources4
Beta strandi691 – 695Combined sources5
Helixi701 – 704Combined sources4
Beta strandi707 – 709Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3FG6X-ray3.00A/B/C/D/E/F/G/H345-715[»]
5A1KX-ray2.90A/B6-349[»]
5A1MX-ray1.81A247-350[»]
ProteinModelPortaliQ9Y6U3.
SMRiQ9Y6U3.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9Y6U3.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati27 – 76Gelsolin-like 1Add BLAST50
Repeati148 – 188Gelsolin-like 2Add BLAST41
Repeati265 – 307Gelsolin-like 3Add BLAST43
Repeati398 – 451Gelsolin-like 4Add BLAST54
Repeati523 – 564Gelsolin-like 5Add BLAST42
Repeati626 – 668Gelsolin-like 6Add BLAST43

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 363Actin-severingSequence analysisAdd BLAST363
Regioni112 – 119Polyphosphoinositide bindingBy similarity8
Regioni138 – 146Polyphosphoinositide bindingBy similarity9
Regioni364 – 715Ca(2+)-dependent actin bindingAdd BLAST352

Sequence similaritiesi

Belongs to the villin/gelsolin family.Curated
Contains 6 gelsolin-like repeats.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0443. Eukaryota.
ENOG410XR0A. LUCA.
GeneTreeiENSGT00760000119111.
HOGENOMiHOG000233630.
HOVERGENiHBG004183.
InParanoidiQ9Y6U3.
KOiK05768.
OMAiGLYHEEF.
OrthoDBiEOG091G05SC.
PhylomeDBiQ9Y6U3.
TreeFamiTF313468.

Family and domain databases

Gene3Di3.40.20.10. 6 hits.
InterProiIPR029006. ADF-H/Gelsolin-like_dom.
IPR030012. Adseverin.
IPR007123. Gelsolin-like_dom.
IPR007122. Villin/Gelsolin.
[Graphical view]
PANTHERiPTHR11977. PTHR11977. 1 hit.
PTHR11977:SF14. PTHR11977:SF14. 1 hit.
PfamiPF00626. Gelsolin. 6 hits.
[Graphical view]
PRINTSiPR00597. GELSOLIN.
SMARTiSM00262. GEL. 6 hits.
[Graphical view]
SUPFAMiSSF82754. SSF82754. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9Y6U3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MARELYHEEF ARAGKQAGLQ VWRIEKLELV PVPQSAHGDF YVGDAYLVLH
60 70 80 90 100
TAKTSRGFTY HLHFWLGKEC SQDESTAAAI FTVQMDDYLG GKPVQNRELQ
110 120 130 140 150
GYESNDFVSY FKGGLKYKAG GVASGLNHVL TNDLTAKRLL HVKGRRVVRA
160 170 180 190 200
TEVPLSWDSF NKGDCFIIDL GTEIYQWCGS SCNKYERLKA NQVATGIRYN
210 220 230 240 250
ERKGRSELIV VEEGSEPSEL IKVLGEKPEL PDGGDDDDII ADISNRKMAK
260 270 280 290 300
LYMVSDASGS MRVTVVAEEN PFSMAMLLSE ECFILDHGAA KQIFVWKGKD
310 320 330 340 350
ANPQERKAAM KTAEEFLQQM NYSKNTQIQV LPEGGETPIF KQFFKDWRDK
360 370 380 390 400
DQSDGFGKVY VTEKVAQIKQ IPFDASKLHS SPQMAAQHNM VDDGSGKVEI
410 420 430 440 450
WRVENNGRIQ VDQNSYGEFY GGDCYIILYT YPRGQIIYTW QGANATRDEL
460 470 480 490 500
TTSAFLTVQL DRSLGGQAVQ IRVSQGKEPV HLLSLFKDKP LIIYKNGTSK
510 520 530 540 550
KGGQAPAPPT RLFQVRRNLA SITRIVEVDV DANSLNSNDV FVLKLPQNSG
560 570 580 590 600
YIWVGKGASQ EEEKGAEYVA SVLKCKTLRI QEGEEPEEFW NSLGGKKDYQ
610 620 630 640 650
TSPLLETQAE DHPPRLYGCS NKTGRFVIEE IPGEFTQDDL AEDDVMLLDA
660 670 680 690 700
WEQIFIWIGK DANEVEKKES LKSAKMYLET DPSGRDKRTP IVIIKQGHEP
710
PTFTGWFLGW DSSKW
Length:715
Mass (Da):80,489
Last modified:January 4, 2005 - v4
Checksum:i20420C82DB2E2D24
GO
Isoform 2 (identifier: Q9Y6U3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     528-580: VDVDANSLNS...SVLKCKTLRI → RSSGIPLEGK...RFQESSPRMI
     581-715: Missing.

Note: No experimental confirmation available.
Show »
Length:580
Mass (Da):65,357
Checksum:i4291B928419AB445
GO
Isoform 3 (identifier: Q9Y6U3-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-247: Missing.

Show »
Length:468
Mass (Da):52,814
Checksum:iFB1BAE7D65C7E39F
GO

Sequence cautioni

The sequence BAB67798 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti48V → M in BAC11416 (PubMed:14702039).Curated1
Sequence conflicti476G → D in AAK60494 (Ref. 1) Curated1
Sequence conflicti546P → S in AAK60494 (Ref. 1) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_05995661H → R.1 PublicationCorresponds to variant rs2240572dbSNPEnsembl.1
Natural variantiVAR_059957443A → P.Corresponds to variant rs35083013dbSNPEnsembl.1
Natural variantiVAR_057470455F → L.Corresponds to variant rs17166250dbSNPEnsembl.1
Natural variantiVAR_059958500K → R.Corresponds to variant rs35705332dbSNPEnsembl.1
Natural variantiVAR_057471578L → F.Corresponds to variant rs1138957dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0405481 – 247Missing in isoform 3. 1 PublicationAdd BLAST247
Alternative sequenceiVSP_012427528 – 580VDVDA…KTLRI → RSSGIPLEGKKTTRPHHYWK PRLKTIHLGFTAALTKLEDL LLKRFQESSPRMI in isoform 2. 1 PublicationAdd BLAST53
Alternative sequenceiVSP_012428581 – 715Missing in isoform 2. 1 PublicationAdd BLAST135

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF276507 mRNA. Translation: AAK60494.1.
AK027778 mRNA. Translation: BAB55361.1.
AK075123 mRNA. Translation: BAC11416.1.
AK290363 mRNA. Translation: BAF83052.1.
AB067492 mRNA. Translation: BAB67798.1. Different initiation.
AC005281 Genomic DNA. Translation: AAD15423.1.
CH471073 Genomic DNA. Translation: EAW93647.1.
BC021090 mRNA. Translation: AAH21090.1.
BU193785 mRNA. No translation available.
CCDSiCCDS47545.1. [Q9Y6U3-1]
CCDS47546.1. [Q9Y6U3-3]
RefSeqiNP_001106177.1. NM_001112706.2. [Q9Y6U3-1]
NP_149119.1. NM_033128.3. [Q9Y6U3-3]
UniGeneiHs.633359.
Hs.655515.

Genome annotation databases

EnsembliENST00000297029; ENSP00000297029; ENSG00000006747. [Q9Y6U3-1]
ENST00000341757; ENSP00000341375; ENSG00000006747. [Q9Y6U3-2]
ENST00000519209; ENSP00000430997; ENSG00000006747. [Q9Y6U3-3]
GeneIDi85477.
KEGGihsa:85477.
UCSCiuc003ssn.5. human. [Q9Y6U3-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF276507 mRNA. Translation: AAK60494.1.
AK027778 mRNA. Translation: BAB55361.1.
AK075123 mRNA. Translation: BAC11416.1.
AK290363 mRNA. Translation: BAF83052.1.
AB067492 mRNA. Translation: BAB67798.1. Different initiation.
AC005281 Genomic DNA. Translation: AAD15423.1.
CH471073 Genomic DNA. Translation: EAW93647.1.
BC021090 mRNA. Translation: AAH21090.1.
BU193785 mRNA. No translation available.
CCDSiCCDS47545.1. [Q9Y6U3-1]
CCDS47546.1. [Q9Y6U3-3]
RefSeqiNP_001106177.1. NM_001112706.2. [Q9Y6U3-1]
NP_149119.1. NM_033128.3. [Q9Y6U3-3]
UniGeneiHs.633359.
Hs.655515.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3FG6X-ray3.00A/B/C/D/E/F/G/H345-715[»]
5A1KX-ray2.90A/B6-349[»]
5A1MX-ray1.81A247-350[»]
ProteinModelPortaliQ9Y6U3.
SMRiQ9Y6U3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi124552. 11 interactors.
IntActiQ9Y6U3. 1 interactor.
MINTiMINT-1141238.
STRINGi9606.ENSP00000297029.

PTM databases

iPTMnetiQ9Y6U3.
PhosphoSitePlusiQ9Y6U3.

Polymorphism and mutation databases

BioMutaiSCIN.
DMDMi57015325.

Proteomic databases

EPDiQ9Y6U3.
PaxDbiQ9Y6U3.
PeptideAtlasiQ9Y6U3.
PRIDEiQ9Y6U3.

Protocols and materials databases

DNASUi85477.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000297029; ENSP00000297029; ENSG00000006747. [Q9Y6U3-1]
ENST00000341757; ENSP00000341375; ENSG00000006747. [Q9Y6U3-2]
ENST00000519209; ENSP00000430997; ENSG00000006747. [Q9Y6U3-3]
GeneIDi85477.
KEGGihsa:85477.
UCSCiuc003ssn.5. human. [Q9Y6U3-1]

Organism-specific databases

CTDi85477.
DisGeNETi85477.
GeneCardsiSCIN.
HGNCiHGNC:21695. SCIN.
HPAiHPA020518.
HPA024264.
MIMi613416. gene.
neXtProtiNX_Q9Y6U3.
OpenTargetsiENSG00000006747.
PharmGKBiPA134981389.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0443. Eukaryota.
ENOG410XR0A. LUCA.
GeneTreeiENSGT00760000119111.
HOGENOMiHOG000233630.
HOVERGENiHBG004183.
InParanoidiQ9Y6U3.
KOiK05768.
OMAiGLYHEEF.
OrthoDBiEOG091G05SC.
PhylomeDBiQ9Y6U3.
TreeFamiTF313468.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000006747-MONOMER.

Miscellaneous databases

ChiTaRSiSCIN. human.
EvolutionaryTraceiQ9Y6U3.
GeneWikiiSCIN.
GenomeRNAii85477.
PROiQ9Y6U3.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000006747.
CleanExiHS_SCIN.
ExpressionAtlasiQ9Y6U3. baseline and differential.
GenevisibleiQ9Y6U3. HS.

Family and domain databases

Gene3Di3.40.20.10. 6 hits.
InterProiIPR029006. ADF-H/Gelsolin-like_dom.
IPR030012. Adseverin.
IPR007123. Gelsolin-like_dom.
IPR007122. Villin/Gelsolin.
[Graphical view]
PANTHERiPTHR11977. PTHR11977. 1 hit.
PTHR11977:SF14. PTHR11977:SF14. 1 hit.
PfamiPF00626. Gelsolin. 6 hits.
[Graphical view]
PRINTSiPR00597. GELSOLIN.
SMARTiSM00262. GEL. 6 hits.
[Graphical view]
SUPFAMiSSF82754. SSF82754. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiADSV_HUMAN
AccessioniPrimary (citable) accession number: Q9Y6U3
Secondary accession number(s): A8K2U8
, Q8NBZ6, Q8WU97, Q96JC7, Q96PY2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: January 4, 2005
Last modified: November 30, 2016
This is version 150 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.