Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q9Y6T7

- DGKB_HUMAN

UniProt

Q9Y6T7 - DGKB_HUMAN

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Diacylglycerol kinase beta

Gene

DGKB

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Exhibits high phosphorylation activity for long-chain diacylglycerols.By similarity

Catalytic activityi

ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.

Enzyme regulationi

Stimulated by phosphatidylserine.By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi162 – 173121PROSITE-ProRule annotationAdd
BLAST
Calcium bindingi207 – 218122PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri244 – 29451Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri309 – 35850Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. calcium ion binding Source: InterPro
  3. diacylglycerol kinase activity Source: UniProtKB-EC
  4. NAD+ kinase activity Source: InterPro

GO - Biological processi

  1. blood coagulation Source: Reactome
  2. intracellular signal transduction Source: InterPro
  3. platelet activation Source: Reactome
  4. protein kinase C-activating G-protein coupled receptor signaling pathway Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Calcium, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_2202. Effects of PIP2 hydrolysis.
SABIO-RKQ9Y6T7.

Names & Taxonomyi

Protein namesi
Recommended name:
Diacylglycerol kinase beta (EC:2.7.1.107)
Short name:
DAG kinase beta
Alternative name(s):
90 kDa diacylglycerol kinase
Diglyceride kinase beta
Short name:
DGK-beta
Gene namesi
Name:DGKB
Synonyms:DAGK2, KIAA0718
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 7

Organism-specific databases

HGNCiHGNC:2850. DGKB.

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. plasma membrane Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA27311.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 804804Diacylglycerol kinase betaPRO_0000218457Add
BLAST

Proteomic databases

MaxQBiQ9Y6T7.
PaxDbiQ9Y6T7.
PRIDEiQ9Y6T7.

PTM databases

PhosphoSiteiQ9Y6T7.

Expressioni

Gene expression databases

BgeeiQ9Y6T7.
ExpressionAtlasiQ9Y6T7. baseline and differential.
GenevestigatoriQ9Y6T7.

Interactioni

Protein-protein interaction databases

BioGridi107977. 2 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ9Y6T7.
SMRiQ9Y6T7. Positions 10-222, 245-286.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini149 – 18436EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini194 – 22936EF-hand 2PROSITE-ProRule annotationAdd
BLAST
Domaini434 – 568135DAGKcPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 DAGKc domain.PROSITE-ProRule annotation
Contains 2 EF-hand domains.PROSITE-ProRule annotation
Contains 2 phorbol-ester/DAG-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri244 – 29451Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri309 – 35850Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiNOG47311.
GeneTreeiENSGT00760000119050.
HOVERGENiHBG051345.
InParanoidiQ9Y6T7.
KOiK00901.
OMAiCIEKANL.
OrthoDBiEOG75XGK8.
PhylomeDBiQ9Y6T7.
TreeFamiTF313104.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
1.10.238.110. 1 hit.
InterProiIPR016064. ATP-NAD_kinase_PpnK-typ.
IPR029477. DAG_kinase_typeI_N.
IPR000756. Diacylglycerol_kin_accessory.
IPR001206. Diacylglycerol_kinase_cat_dom.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR002219. PE/DAG-bd.
[Graphical view]
PfamiPF00130. C1_1. 2 hits.
PF14513. DAG_kinase_N. 1 hit.
PF00609. DAGK_acc. 1 hit.
PF00781. DAGK_cat. 1 hit.
PF13499. EF-hand_7. 1 hit.
[Graphical view]
SMARTiSM00109. C1. 2 hits.
SM00045. DAGKa. 1 hit.
SM00046. DAGKc. 1 hit.
SM00054. EFh. 2 hits.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 2 hits.
PROSITEiPS50146. DAGK. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 2 hits.
PS00479. ZF_DAG_PE_1. 2 hits.
PS50081. ZF_DAG_PE_2. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9Y6T7-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTNQEKWAHL SPSEFSQLQK YAEYSTKKLK DVLEEFHGNG VLAKYNPEGK
60 70 80 90 100
QDILNQTIDF EGFKLFMKTF LEAELPDDFT AHLFMSFSNK FPHSSPMVKS
110 120 130 140 150
KPALLSGGLR MNKGAITPPR TTSPANTCSP EVIHLKDIVC YLSLLERGRP
160 170 180 190 200
EDKLEFMFRL YDTDGNGFLD SSELENIISQ MMHVAEYLEW DVTELNPILH
210 220 230 240 250
EMMEEIDYDH DGTVSLEEWI QGGMTTIPLL VLLGLENNVK DDGQHVWRLK
260 270 280 290 300
HFNKPAYCNL CLNMLIGVGK QGLCCSFCKY TVHERCVARA PPSCIKTYVK
310 320 330 340 350
SKRNTDVMHH YWVEGNCPTK CDKCHKTVKC YQGLTGLHCV WCQITLHNKC
360 370 380 390 400
ASHLKPECDC GPLKDHILPP TTICPVVLQT LPTSGVSVPE ERQSTVKKEK
410 420 430 440 450
SGSQQPNKVI DKNKMQRANS VTVDGQGLQV TPVPGTHPLL VFVNPKSGGK
460 470 480 490 500
QGERIYRKFQ YLLNPRQVYS LSGNGPMPGL NFFRDVPDFR VLACGGDGTV
510 520 530 540 550
GWVLDCIEKA NVGKHPPVAI LPLGTGNDLA RCLRWGGGYE GENLMKILKD
560 570 580 590 600
IENSTEIMLD RWKFEVIPND KDEKGDPVPY SIINNYFSIG VDASIAHRFH
610 620 630 640 650
IMREKHPEKF NSRMKNKFWY FEFGTSETFS ATCKKLHESV EIECDGVQID
660 670 680 690 700
LINISLEGIA ILNIPSMHGG SNLWGESKKR RSHRRIEKKG SDKRTTVTDA
710 720 730 740 750
KELKFASQDL SDQLLEVVGL EGAMEMGQIY TGLKSAGRRL AQCSCVVIRT
760 770 780 790 800
SKSLPMQIDG EPWMQTPCTI KITHKNQAPM LMGPPPKTGL FCSLVKRTRN

RSKE
Length:804
Mass (Da):90,595
Last modified:January 11, 2001 - v2
Checksum:iCBCABD2339BFE176
GO
Isoform 2 (identifier: Q9Y6T7-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     770-773: IKIT → VSTE
     774-804: Missing.

Note: No experimental confirmation available.

Show »
Length:773
Mass (Da):87,051
Checksum:i9AF7118E642F8EB8
GO

Sequence cautioni

The sequence AAS07533.1 differs from that shown. Reason: Erroneous gene model prediction.

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti789 – 7891G → S.
Corresponds to variant rs34616903 [ dbSNP | Ensembl ].
VAR_048858

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei770 – 7734IKIT → VSTE in isoform 2. 1 PublicationVSP_021898
Alternative sequencei774 – 80431Missing in isoform 2. 1 PublicationVSP_021899Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AX032742 mRNA. Translation: CAC09945.1.
AC005039 Genomic DNA. Translation: AAS07495.1.
AC005248 Genomic DNA. Translation: AAC25525.1.
AC006150 Genomic DNA. No translation available.
AC011229 Genomic DNA. Translation: AAP21868.1.
AC073258 Genomic DNA. Translation: AAS07533.1. Sequence problems.
AC105459 Genomic DNA. Translation: AAP22362.1.
CH236948 Genomic DNA. Translation: EAL24292.1.
CH236948 Genomic DNA. Translation: EAL24293.1.
CH471073 Genomic DNA. Translation: EAW93663.1.
CH471073 Genomic DNA. Translation: EAW93664.1.
BC105005 mRNA. Translation: AAI05006.1.
AB018261 mRNA. Translation: BAA34438.1.
CCDSiCCDS47547.1. [Q9Y6T7-1]
CCDS47548.1. [Q9Y6T7-2]
RefSeqiNP_004071.1. NM_004080.2. [Q9Y6T7-1]
NP_663733.1. NM_145695.2. [Q9Y6T7-2]
XP_005249685.1. XM_005249628.1. [Q9Y6T7-1]
UniGeneiHs.567255.

Genome annotation databases

EnsembliENST00000399322; ENSP00000382260; ENSG00000136267. [Q9Y6T7-1]
ENST00000403951; ENSP00000385780; ENSG00000136267. [Q9Y6T7-1]
ENST00000406247; ENSP00000386066; ENSG00000136267. [Q9Y6T7-2]
GeneIDi1607.
KEGGihsa:1607.
UCSCiuc003ssz.3. human. [Q9Y6T7-1]
uc003sta.3. human. [Q9Y6T7-2]

Polymorphism databases

DMDMi12643960.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AX032742 mRNA. Translation: CAC09945.1 .
AC005039 Genomic DNA. Translation: AAS07495.1 .
AC005248 Genomic DNA. Translation: AAC25525.1 .
AC006150 Genomic DNA. No translation available.
AC011229 Genomic DNA. Translation: AAP21868.1 .
AC073258 Genomic DNA. Translation: AAS07533.1 . Sequence problems.
AC105459 Genomic DNA. Translation: AAP22362.1 .
CH236948 Genomic DNA. Translation: EAL24292.1 .
CH236948 Genomic DNA. Translation: EAL24293.1 .
CH471073 Genomic DNA. Translation: EAW93663.1 .
CH471073 Genomic DNA. Translation: EAW93664.1 .
BC105005 mRNA. Translation: AAI05006.1 .
AB018261 mRNA. Translation: BAA34438.1 .
CCDSi CCDS47547.1. [Q9Y6T7-1 ]
CCDS47548.1. [Q9Y6T7-2 ]
RefSeqi NP_004071.1. NM_004080.2. [Q9Y6T7-1 ]
NP_663733.1. NM_145695.2. [Q9Y6T7-2 ]
XP_005249685.1. XM_005249628.1. [Q9Y6T7-1 ]
UniGenei Hs.567255.

3D structure databases

ProteinModelPortali Q9Y6T7.
SMRi Q9Y6T7. Positions 10-222, 245-286.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 107977. 2 interactions.

PTM databases

PhosphoSitei Q9Y6T7.

Polymorphism databases

DMDMi 12643960.

Proteomic databases

MaxQBi Q9Y6T7.
PaxDbi Q9Y6T7.
PRIDEi Q9Y6T7.

Protocols and materials databases

DNASUi 1607.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000399322 ; ENSP00000382260 ; ENSG00000136267 . [Q9Y6T7-1 ]
ENST00000403951 ; ENSP00000385780 ; ENSG00000136267 . [Q9Y6T7-1 ]
ENST00000406247 ; ENSP00000386066 ; ENSG00000136267 . [Q9Y6T7-2 ]
GeneIDi 1607.
KEGGi hsa:1607.
UCSCi uc003ssz.3. human. [Q9Y6T7-1 ]
uc003sta.3. human. [Q9Y6T7-2 ]

Organism-specific databases

CTDi 1607.
GeneCardsi GC07M014153.
HGNCi HGNC:2850. DGKB.
MIMi 604070. gene.
neXtProti NX_Q9Y6T7.
PharmGKBi PA27311.
HUGEi Search...
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG47311.
GeneTreei ENSGT00760000119050.
HOVERGENi HBG051345.
InParanoidi Q9Y6T7.
KOi K00901.
OMAi CIEKANL.
OrthoDBi EOG75XGK8.
PhylomeDBi Q9Y6T7.
TreeFami TF313104.

Enzyme and pathway databases

Reactomei REACT_2202. Effects of PIP2 hydrolysis.
SABIO-RK Q9Y6T7.

Miscellaneous databases

ChiTaRSi Dgkb. human.
GeneWikii DGKB.
GenomeRNAii 1607.
NextBioi 6600.
PROi Q9Y6T7.
SOURCEi Search...

Gene expression databases

Bgeei Q9Y6T7.
ExpressionAtlasi Q9Y6T7. baseline and differential.
Genevestigatori Q9Y6T7.

Family and domain databases

Gene3Di 1.10.238.10. 1 hit.
1.10.238.110. 1 hit.
InterProi IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR029477. DAG_kinase_typeI_N.
IPR000756. Diacylglycerol_kin_accessory.
IPR001206. Diacylglycerol_kinase_cat_dom.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR002219. PE/DAG-bd.
[Graphical view ]
Pfami PF00130. C1_1. 2 hits.
PF14513. DAG_kinase_N. 1 hit.
PF00609. DAGK_acc. 1 hit.
PF00781. DAGK_cat. 1 hit.
PF13499. EF-hand_7. 1 hit.
[Graphical view ]
SMARTi SM00109. C1. 2 hits.
SM00045. DAGKa. 1 hit.
SM00046. DAGKc. 1 hit.
SM00054. EFh. 2 hits.
[Graphical view ]
SUPFAMi SSF111331. SSF111331. 2 hits.
PROSITEi PS50146. DAGK. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 2 hits.
PS00479. ZF_DAG_PE_1. 2 hits.
PS50081. ZF_DAG_PE_2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The DNA sequence of human chromosome 7."
    Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L.
    , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K.
    Nature 424:157-164(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "Human chromosome 7: DNA sequence and biology."
    Scherer S.W., Cheung J., MacDonald J.R., Osborne L.R., Nakabayashi K., Herbrick J.-A., Carson A.R., Parker-Katiraee L., Skaug J., Khaja R., Zhang J., Hudek A.K., Li M., Haddad M., Duggan G.E., Fernandez B.A., Kanematsu E., Gentles S.
    , Christopoulos C.C., Choufani S., Kwasnicka D., Zheng X.H., Lai Z., Nusskern D.R., Zhang Q., Gu Z., Lu F., Zeesman S., Nowaczyk M.J., Teshima I., Chitayat D., Shuman C., Weksberg R., Zackai E.H., Grebe T.A., Cox S.R., Kirkpatrick S.J., Rahman N., Friedman J.M., Heng H.H.Q., Pelicci P.G., Lo-Coco F., Belloni E., Shaffer L.G., Pober B., Morton C.C., Gusella J.F., Bruns G.A.P., Korf B.R., Quade B.J., Ligon A.H., Ferguson H., Higgins A.W., Leach N.T., Herrick S.R., Lemyre E., Farra C.G., Kim H.-G., Summers A.M., Gripp K.W., Roberts W., Szatmari P., Winsor E.J.T., Grzeschik K.-H., Teebi A., Minassian B.A., Kere J., Armengol L., Pujana M.A., Estivill X., Wilson M.D., Koop B.F., Tosi S., Moore G.E., Boright A.P., Zlotorynski E., Kerem B., Kroisel P.M., Petek E., Oscier D.G., Mould S.J., Doehner H., Doehner K., Rommens J.M., Vincent J.B., Venter J.C., Li P.W., Mural R.J., Adams M.D., Tsui L.-C.
    Science 300:767-772(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Brain.
  6. "Prediction of the coding sequences of unidentified human genes. XI. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
    Nagase T., Ishikawa K., Suyama M., Kikuno R., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.
    DNA Res. 5:277-286(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 63-804 (ISOFORM 1).
    Tissue: Brain.

Entry informationi

Entry nameiDGKB_HUMAN
AccessioniPrimary (citable) accession number: Q9Y6T7
Secondary accession number(s): A4D116
, A4D117, A8MXU2, O75241, Q2M377, Q75MF9, Q75MU7, Q86UI5, Q86UM9, Q9UQ29
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: January 11, 2001
Last modified: October 29, 2014
This is version 131 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3