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Protein

Diacylglycerol kinase beta

Gene

DGKB

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Exhibits high phosphorylation activity for long-chain diacylglycerols.By similarity

Catalytic activityi

ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.

Enzyme regulationi

Stimulated by phosphatidylserine.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi162 – 1731PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi207 – 2182PROSITE-ProRule annotationAdd BLAST12
Zinc fingeri244 – 294Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd BLAST51
Zinc fingeri309 – 358Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd BLAST50

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
LigandATP-binding, Calcium, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BRENDAi2.7.1.107 2681
ReactomeiR-HSA-114508 Effects of PIP2 hydrolysis
SABIO-RKiQ9Y6T7

Chemistry databases

SwissLipidsiSLP:000000915 [Q9Y6T7-1]
SLP:000000916 [Q9Y6T7-2]

Names & Taxonomyi

Protein namesi
Recommended name:
Diacylglycerol kinase beta (EC:2.7.1.107)
Short name:
DAG kinase beta
Alternative name(s):
90 kDa diacylglycerol kinase
Diglyceride kinase beta
Short name:
DGK-beta
Gene namesi
Name:DGKB
Synonyms:DAGK2, KIAA0718
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 7

Organism-specific databases

EuPathDBiHostDB:ENSG00000136267.13
HGNCiHGNC:2850 DGKB
MIMi604070 gene
neXtProtiNX_Q9Y6T7

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

DisGeNETi1607
OpenTargetsiENSG00000136267
PharmGKBiPA27311

Polymorphism and mutation databases

BioMutaiDGKB
DMDMi12643960

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002184571 – 804Diacylglycerol kinase betaAdd BLAST804

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei117PhosphothreonineBy similarity1
Modified residuei420PhosphoserineBy similarity1
Modified residuei793PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9Y6T7
PeptideAtlasiQ9Y6T7
PRIDEiQ9Y6T7

PTM databases

iPTMnetiQ9Y6T7
PhosphoSitePlusiQ9Y6T7

Expressioni

Gene expression databases

BgeeiENSG00000136267
ExpressionAtlasiQ9Y6T7 baseline and differential
GenevisibleiQ9Y6T7 HS

Organism-specific databases

HPAiHPA020321

Interactioni

Protein-protein interaction databases

BioGridi107977, 2 interactors
STRINGi9606.ENSP00000382260

Structurei

3D structure databases

ProteinModelPortaliQ9Y6T7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini149 – 184EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini194 – 229EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini434 – 568DAGKcPROSITE-ProRule annotationAdd BLAST135

Sequence similaritiesi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri244 – 294Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd BLAST51
Zinc fingeri309 – 358Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd BLAST50

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1169 Eukaryota
ENOG410XQVB LUCA
GeneTreeiENSGT00760000119050
HOVERGENiHBG051345
InParanoidiQ9Y6T7
KOiK00901
OMAiTDKNKMQ
OrthoDBiEOG091G04A5
PhylomeDBiQ9Y6T7
TreeFamiTF313104

Family and domain databases

CDDicd00029 C1, 2 hits
cd00051 EFh, 1 hit
Gene3Di1.10.238.110, 2 hits
3.40.50.10330, 1 hit
InterProiView protein in InterPro
IPR017438 ATP-NAD_kinase_N
IPR029477 DAG_kinase_typeI_N
IPR037607 DGK
IPR038199 DGK_typeI_N_sf
IPR000756 Diacylglycerol_kin_accessory
IPR001206 Diacylglycerol_kinase_cat_dom
IPR011992 EF-hand-dom_pair
IPR018247 EF_Hand_1_Ca_BS
IPR002048 EF_hand_dom
IPR016064 NAD/diacylglycerol_kinase_sf
IPR002219 PE/DAG-bd
PANTHERiPTHR11255 PTHR11255, 1 hit
PfamiView protein in Pfam
PF00130 C1_1, 2 hits
PF14513 DAG_kinase_N, 2 hits
PF00609 DAGK_acc, 1 hit
PF00781 DAGK_cat, 1 hit
PF13499 EF-hand_7, 1 hit
SMARTiView protein in SMART
SM00109 C1, 2 hits
SM00045 DAGKa, 1 hit
SM00046 DAGKc, 1 hit
SM00054 EFh, 2 hits
SUPFAMiSSF111331 SSF111331, 2 hits
SSF47473 SSF47473, 3 hits
PROSITEiView protein in PROSITE
PS50146 DAGK, 1 hit
PS00018 EF_HAND_1, 2 hits
PS50222 EF_HAND_2, 2 hits
PS00479 ZF_DAG_PE_1, 2 hits
PS50081 ZF_DAG_PE_2, 2 hits

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9Y6T7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTNQEKWAHL SPSEFSQLQK YAEYSTKKLK DVLEEFHGNG VLAKYNPEGK
60 70 80 90 100
QDILNQTIDF EGFKLFMKTF LEAELPDDFT AHLFMSFSNK FPHSSPMVKS
110 120 130 140 150
KPALLSGGLR MNKGAITPPR TTSPANTCSP EVIHLKDIVC YLSLLERGRP
160 170 180 190 200
EDKLEFMFRL YDTDGNGFLD SSELENIISQ MMHVAEYLEW DVTELNPILH
210 220 230 240 250
EMMEEIDYDH DGTVSLEEWI QGGMTTIPLL VLLGLENNVK DDGQHVWRLK
260 270 280 290 300
HFNKPAYCNL CLNMLIGVGK QGLCCSFCKY TVHERCVARA PPSCIKTYVK
310 320 330 340 350
SKRNTDVMHH YWVEGNCPTK CDKCHKTVKC YQGLTGLHCV WCQITLHNKC
360 370 380 390 400
ASHLKPECDC GPLKDHILPP TTICPVVLQT LPTSGVSVPE ERQSTVKKEK
410 420 430 440 450
SGSQQPNKVI DKNKMQRANS VTVDGQGLQV TPVPGTHPLL VFVNPKSGGK
460 470 480 490 500
QGERIYRKFQ YLLNPRQVYS LSGNGPMPGL NFFRDVPDFR VLACGGDGTV
510 520 530 540 550
GWVLDCIEKA NVGKHPPVAI LPLGTGNDLA RCLRWGGGYE GENLMKILKD
560 570 580 590 600
IENSTEIMLD RWKFEVIPND KDEKGDPVPY SIINNYFSIG VDASIAHRFH
610 620 630 640 650
IMREKHPEKF NSRMKNKFWY FEFGTSETFS ATCKKLHESV EIECDGVQID
660 670 680 690 700
LINISLEGIA ILNIPSMHGG SNLWGESKKR RSHRRIEKKG SDKRTTVTDA
710 720 730 740 750
KELKFASQDL SDQLLEVVGL EGAMEMGQIY TGLKSAGRRL AQCSCVVIRT
760 770 780 790 800
SKSLPMQIDG EPWMQTPCTI KITHKNQAPM LMGPPPKTGL FCSLVKRTRN

RSKE
Length:804
Mass (Da):90,595
Last modified:January 11, 2001 - v2
Checksum:iCBCABD2339BFE176
GO
Isoform 2 (identifier: Q9Y6T7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     770-773: IKIT → VSTE
     774-804: Missing.

Note: No experimental confirmation available.
Show »
Length:773
Mass (Da):87,051
Checksum:i9AF7118E642F8EB8
GO

Sequence cautioni

The sequence AAS07533 differs from that shown. Reason: Erroneous gene model prediction.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_048858789G → S. Corresponds to variant dbSNP:rs34616903Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_021898770 – 773IKIT → VSTE in isoform 2. 1 Publication4
Alternative sequenceiVSP_021899774 – 804Missing in isoform 2. 1 PublicationAdd BLAST31

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AX032742 mRNA Translation: CAC09945.1
AC005039 Genomic DNA Translation: AAS07495.1
AC005248 Genomic DNA Translation: AAC25525.1
AC006150 Genomic DNA No translation available.
AC011229 Genomic DNA Translation: AAP21868.1
AC073258 Genomic DNA Translation: AAS07533.1 Sequence problems.
AC105459 Genomic DNA Translation: AAP22362.1
CH236948 Genomic DNA Translation: EAL24292.1
CH236948 Genomic DNA Translation: EAL24293.1
CH471073 Genomic DNA Translation: EAW93663.1
CH471073 Genomic DNA Translation: EAW93664.1
BC105005 mRNA Translation: AAI05006.1
AB018261 mRNA Translation: BAA34438.1
CCDSiCCDS47547.1 [Q9Y6T7-1]
CCDS47548.1 [Q9Y6T7-2]
RefSeqiNP_004071.1, NM_004080.2
NP_663733.1, NM_145695.2 [Q9Y6T7-2]
XP_005249685.1, XM_005249628.2
XP_011513455.1, XM_011515153.2
XP_011513456.1, XM_011515154.2 [Q9Y6T7-1]
UniGeneiHs.567255

Genome annotation databases

EnsembliENST00000399322; ENSP00000382260; ENSG00000136267 [Q9Y6T7-1]
ENST00000403951; ENSP00000385780; ENSG00000136267 [Q9Y6T7-1]
ENST00000406247; ENSP00000386066; ENSG00000136267 [Q9Y6T7-2]
GeneIDi1607
KEGGihsa:1607
UCSCiuc003ssz.3 human [Q9Y6T7-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiDGKB_HUMAN
AccessioniPrimary (citable) accession number: Q9Y6T7
Secondary accession number(s): A4D116
, A4D117, A8MXU2, O75241, Q2M377, Q75MF9, Q75MU7, Q86UI5, Q86UM9, Q9UQ29
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: January 11, 2001
Last modified: May 23, 2018
This is version 164 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

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