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Q9Y6T7 (DGKB_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 128. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Diacylglycerol kinase beta

Short name=DAG kinase beta
EC=2.7.1.107
Alternative name(s):
90 kDa diacylglycerol kinase
Diglyceride kinase beta
Short name=DGK-beta
Gene names
Name:DGKB
Synonyms:DAGK2, KIAA0718
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length804 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Exhibits high phosphorylation activity for long-chain diacylglycerols By similarity.

Catalytic activity

ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.

Enzyme regulation

Stimulated by phosphatidylserine By similarity.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the eukaryotic diacylglycerol kinase family.

Contains 1 DAGKc domain.

Contains 2 EF-hand domains.

Contains 2 phorbol-ester/DAG-type zinc fingers.

Sequence caution

The sequence AAS07533.1 differs from that shown. Reason: Erroneous gene model prediction.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9Y6T7-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9Y6T7-2)

The sequence of this isoform differs from the canonical sequence as follows:
     770-773: IKIT → VSTE
     774-804: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 804804Diacylglycerol kinase beta
PRO_0000218457

Regions

Domain149 – 18436EF-hand 1
Domain194 – 22936EF-hand 2
Domain434 – 568135DAGKc
Calcium binding162 – 173121 Potential
Calcium binding207 – 218122 Potential
Zinc finger244 – 29451Phorbol-ester/DAG-type 1
Zinc finger309 – 35850Phorbol-ester/DAG-type 2

Natural variations

Alternative sequence770 – 7734IKIT → VSTE in isoform 2.
VSP_021898
Alternative sequence774 – 80431Missing in isoform 2.
VSP_021899
Natural variant7891G → S.
Corresponds to variant rs34616903 [ dbSNP | Ensembl ].
VAR_048858

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified January 11, 2001. Version 2.
Checksum: CBCABD2339BFE176

FASTA80490,595
        10         20         30         40         50         60 
MTNQEKWAHL SPSEFSQLQK YAEYSTKKLK DVLEEFHGNG VLAKYNPEGK QDILNQTIDF 

        70         80         90        100        110        120 
EGFKLFMKTF LEAELPDDFT AHLFMSFSNK FPHSSPMVKS KPALLSGGLR MNKGAITPPR 

       130        140        150        160        170        180 
TTSPANTCSP EVIHLKDIVC YLSLLERGRP EDKLEFMFRL YDTDGNGFLD SSELENIISQ 

       190        200        210        220        230        240 
MMHVAEYLEW DVTELNPILH EMMEEIDYDH DGTVSLEEWI QGGMTTIPLL VLLGLENNVK 

       250        260        270        280        290        300 
DDGQHVWRLK HFNKPAYCNL CLNMLIGVGK QGLCCSFCKY TVHERCVARA PPSCIKTYVK 

       310        320        330        340        350        360 
SKRNTDVMHH YWVEGNCPTK CDKCHKTVKC YQGLTGLHCV WCQITLHNKC ASHLKPECDC 

       370        380        390        400        410        420 
GPLKDHILPP TTICPVVLQT LPTSGVSVPE ERQSTVKKEK SGSQQPNKVI DKNKMQRANS 

       430        440        450        460        470        480 
VTVDGQGLQV TPVPGTHPLL VFVNPKSGGK QGERIYRKFQ YLLNPRQVYS LSGNGPMPGL 

       490        500        510        520        530        540 
NFFRDVPDFR VLACGGDGTV GWVLDCIEKA NVGKHPPVAI LPLGTGNDLA RCLRWGGGYE 

       550        560        570        580        590        600 
GENLMKILKD IENSTEIMLD RWKFEVIPND KDEKGDPVPY SIINNYFSIG VDASIAHRFH 

       610        620        630        640        650        660 
IMREKHPEKF NSRMKNKFWY FEFGTSETFS ATCKKLHESV EIECDGVQID LINISLEGIA 

       670        680        690        700        710        720 
ILNIPSMHGG SNLWGESKKR RSHRRIEKKG SDKRTTVTDA KELKFASQDL SDQLLEVVGL 

       730        740        750        760        770        780 
EGAMEMGQIY TGLKSAGRRL AQCSCVVIRT SKSLPMQIDG EPWMQTPCTI KITHKNQAPM 

       790        800 
LMGPPPKTGL FCSLVKRTRN RSKE 

« Hide

Isoform 2 [UniParc].

Checksum: 9AF7118E642F8EB8
Show »

FASTA77387,051

References

« Hide 'large scale' references
[1]"Novel proteins."
Caricasole A., Caldara F., Sala C.F.
Patent number WO0047723, 17-AUG-2000
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The DNA sequence of human chromosome 7."
Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L. expand/collapse author list , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K.
Nature 424:157-164(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"Human chromosome 7: DNA sequence and biology."
Scherer S.W., Cheung J., MacDonald J.R., Osborne L.R., Nakabayashi K., Herbrick J.-A., Carson A.R., Parker-Katiraee L., Skaug J., Khaja R., Zhang J., Hudek A.K., Li M., Haddad M., Duggan G.E., Fernandez B.A., Kanematsu E., Gentles S. expand/collapse author list , Christopoulos C.C., Choufani S., Kwasnicka D., Zheng X.H., Lai Z., Nusskern D.R., Zhang Q., Gu Z., Lu F., Zeesman S., Nowaczyk M.J., Teshima I., Chitayat D., Shuman C., Weksberg R., Zackai E.H., Grebe T.A., Cox S.R., Kirkpatrick S.J., Rahman N., Friedman J.M., Heng H.H.Q., Pelicci P.G., Lo-Coco F., Belloni E., Shaffer L.G., Pober B., Morton C.C., Gusella J.F., Bruns G.A.P., Korf B.R., Quade B.J., Ligon A.H., Ferguson H., Higgins A.W., Leach N.T., Herrick S.R., Lemyre E., Farra C.G., Kim H.-G., Summers A.M., Gripp K.W., Roberts W., Szatmari P., Winsor E.J.T., Grzeschik K.-H., Teebi A., Minassian B.A., Kere J., Armengol L., Pujana M.A., Estivill X., Wilson M.D., Koop B.F., Tosi S., Moore G.E., Boright A.P., Zlotorynski E., Kerem B., Kroisel P.M., Petek E., Oscier D.G., Mould S.J., Doehner H., Doehner K., Rommens J.M., Vincent J.B., Venter J.C., Li P.W., Mural R.J., Adams M.D., Tsui L.-C.
Science 300:767-772(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Brain.
[6]"Prediction of the coding sequences of unidentified human genes. XI. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
Nagase T., Ishikawa K., Suyama M., Kikuno R., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.
DNA Res. 5:277-286(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 63-804 (ISOFORM 1).
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AX032742 mRNA. Translation: CAC09945.1.
AC005039 Genomic DNA. Translation: AAS07495.1.
AC005248 Genomic DNA. Translation: AAC25525.1.
AC006150 Genomic DNA. No translation available.
AC011229 Genomic DNA. Translation: AAP21868.1.
AC073258 Genomic DNA. Translation: AAS07533.1. Sequence problems.
AC105459 Genomic DNA. Translation: AAP22362.1.
CH236948 Genomic DNA. Translation: EAL24292.1.
CH236948 Genomic DNA. Translation: EAL24293.1.
CH471073 Genomic DNA. Translation: EAW93663.1.
CH471073 Genomic DNA. Translation: EAW93664.1.
BC105005 mRNA. Translation: AAI05006.1.
AB018261 mRNA. Translation: BAA34438.1.
CCDSCCDS47547.1. [Q9Y6T7-1]
CCDS47548.1. [Q9Y6T7-2]
RefSeqNP_004071.1. NM_004080.2. [Q9Y6T7-1]
NP_663733.1. NM_145695.2. [Q9Y6T7-2]
XP_005249685.1. XM_005249628.1. [Q9Y6T7-1]
UniGeneHs.567255.

3D structure databases

ProteinModelPortalQ9Y6T7.
SMRQ9Y6T7. Positions 10-222, 245-286.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid107977. 2 interactions.

Chemistry

DrugBankDB00144. Phosphatidylserine.

PTM databases

PhosphoSiteQ9Y6T7.

Polymorphism databases

DMDM12643960.

Proteomic databases

MaxQBQ9Y6T7.
PaxDbQ9Y6T7.
PRIDEQ9Y6T7.

Protocols and materials databases

DNASU1607.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000399322; ENSP00000382260; ENSG00000136267. [Q9Y6T7-1]
ENST00000403951; ENSP00000385780; ENSG00000136267. [Q9Y6T7-1]
ENST00000406247; ENSP00000386066; ENSG00000136267. [Q9Y6T7-2]
GeneID1607.
KEGGhsa:1607.
UCSCuc003ssz.3. human. [Q9Y6T7-1]
uc003sta.3. human. [Q9Y6T7-2]

Organism-specific databases

CTD1607.
GeneCardsGC07M014153.
HGNCHGNC:2850. DGKB.
MIM604070. gene.
neXtProtNX_Q9Y6T7.
PharmGKBPA27311.
HUGESearch...
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG47311.
HOVERGENHBG051345.
InParanoidQ9Y6T7.
KOK00901.
OMACIEKANL.
OrthoDBEOG75XGK8.
PhylomeDBQ9Y6T7.
TreeFamTF313104.

Enzyme and pathway databases

ReactomeREACT_111102. Signal Transduction.
REACT_604. Hemostasis.
SABIO-RKQ9Y6T7.

Gene expression databases

ArrayExpressQ9Y6T7.
BgeeQ9Y6T7.
GenevestigatorQ9Y6T7.

Family and domain databases

Gene3D1.10.238.10. 1 hit.
1.10.238.110. 1 hit.
InterProIPR016064. ATP-NAD_kinase_PpnK-typ.
IPR029477. DAG_kinase_typeI_N.
IPR000756. Diacylglycerol_kin_accessory.
IPR001206. Diacylglycerol_kinase_cat_dom.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
[Graphical view]
PfamPF00130. C1_1. 2 hits.
PF14513. DAG_kinase_N. 1 hit.
PF00609. DAGK_acc. 1 hit.
PF00781. DAGK_cat. 1 hit.
PF13499. EF-hand_7. 1 hit.
[Graphical view]
SMARTSM00109. C1. 2 hits.
SM00045. DAGKa. 1 hit.
SM00046. DAGKc. 1 hit.
SM00054. EFh. 2 hits.
[Graphical view]
SUPFAMSSF111331. SSF111331. 2 hits.
PROSITEPS50146. DAGK. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 2 hits.
PS00479. ZF_DAG_PE_1. 2 hits.
PS50081. ZF_DAG_PE_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSDgkb. human.
GeneWikiDGKB.
GenomeRNAi1607.
NextBio6600.
PROQ9Y6T7.
SOURCESearch...

Entry information

Entry nameDGKB_HUMAN
AccessionPrimary (citable) accession number: Q9Y6T7
Secondary accession number(s): A4D116 expand/collapse secondary AC list , A4D117, A8MXU2, O75241, Q2M377, Q75MF9, Q75MU7, Q86UI5, Q86UM9, Q9UQ29
Entry history
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: January 11, 2001
Last modified: July 9, 2014
This is version 128 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 7

Human chromosome 7: entries, gene names and cross-references to MIM