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Q9Y6T7

- DGKB_HUMAN

UniProt

Q9Y6T7 - DGKB_HUMAN

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Protein
Diacylglycerol kinase beta
Gene
DGKB, DAGK2, KIAA0718
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Exhibits high phosphorylation activity for long-chain diacylglycerols By similarity.

Catalytic activityi

ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.

Enzyme regulationi

Stimulated by phosphatidylserine By similarity.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi162 – 173121 Reviewed prediction
Add
BLAST
Calcium bindingi207 – 218122 Reviewed prediction
Add
BLAST
Zinc fingeri244 – 29451Phorbol-ester/DAG-type 1
Add
BLAST
Zinc fingeri309 – 35850Phorbol-ester/DAG-type 2
Add
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. NAD+ kinase activity Source: InterPro
  3. calcium ion binding Source: InterPro
  4. diacylglycerol kinase activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

  1. blood coagulation Source: Reactome
  2. intracellular signal transduction Source: InterPro
  3. platelet activation Source: Reactome
  4. protein kinase C-activating G-protein coupled receptor signaling pathway Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Calcium, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_2202. Effects of PIP2 hydrolysis.
SABIO-RKiQ9Y6T7.

Names & Taxonomyi

Protein namesi
Recommended name:
Diacylglycerol kinase beta (EC:2.7.1.107)
Short name:
DAG kinase beta
Alternative name(s):
90 kDa diacylglycerol kinase
Diglyceride kinase beta
Short name:
DGK-beta
Gene namesi
Name:DGKB
Synonyms:DAGK2, KIAA0718
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 7

Organism-specific databases

HGNCiHGNC:2850. DGKB.

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. plasma membrane Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA27311.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 804804Diacylglycerol kinase beta
PRO_0000218457Add
BLAST

Proteomic databases

MaxQBiQ9Y6T7.
PaxDbiQ9Y6T7.
PRIDEiQ9Y6T7.

PTM databases

PhosphoSiteiQ9Y6T7.

Expressioni

Gene expression databases

ArrayExpressiQ9Y6T7.
BgeeiQ9Y6T7.
GenevestigatoriQ9Y6T7.

Interactioni

Protein-protein interaction databases

BioGridi107977. 2 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ9Y6T7.
SMRiQ9Y6T7. Positions 10-222, 245-286.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini149 – 18436EF-hand 1
Add
BLAST
Domaini194 – 22936EF-hand 2
Add
BLAST
Domaini434 – 568135DAGKc
Add
BLAST

Sequence similaritiesi

Contains 1 DAGKc domain.
Contains 2 EF-hand domains.

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiNOG47311.
HOVERGENiHBG051345.
InParanoidiQ9Y6T7.
KOiK00901.
OMAiCIEKANL.
OrthoDBiEOG75XGK8.
PhylomeDBiQ9Y6T7.
TreeFamiTF313104.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
1.10.238.110. 1 hit.
InterProiIPR016064. ATP-NAD_kinase_PpnK-typ.
IPR029477. DAG_kinase_typeI_N.
IPR000756. Diacylglycerol_kin_accessory.
IPR001206. Diacylglycerol_kinase_cat_dom.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
[Graphical view]
PfamiPF00130. C1_1. 2 hits.
PF14513. DAG_kinase_N. 1 hit.
PF00609. DAGK_acc. 1 hit.
PF00781. DAGK_cat. 1 hit.
PF13499. EF-hand_7. 1 hit.
[Graphical view]
SMARTiSM00109. C1. 2 hits.
SM00045. DAGKa. 1 hit.
SM00046. DAGKc. 1 hit.
SM00054. EFh. 2 hits.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 2 hits.
PROSITEiPS50146. DAGK. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 2 hits.
PS00479. ZF_DAG_PE_1. 2 hits.
PS50081. ZF_DAG_PE_2. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9Y6T7-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MTNQEKWAHL SPSEFSQLQK YAEYSTKKLK DVLEEFHGNG VLAKYNPEGK    50
QDILNQTIDF EGFKLFMKTF LEAELPDDFT AHLFMSFSNK FPHSSPMVKS 100
KPALLSGGLR MNKGAITPPR TTSPANTCSP EVIHLKDIVC YLSLLERGRP 150
EDKLEFMFRL YDTDGNGFLD SSELENIISQ MMHVAEYLEW DVTELNPILH 200
EMMEEIDYDH DGTVSLEEWI QGGMTTIPLL VLLGLENNVK DDGQHVWRLK 250
HFNKPAYCNL CLNMLIGVGK QGLCCSFCKY TVHERCVARA PPSCIKTYVK 300
SKRNTDVMHH YWVEGNCPTK CDKCHKTVKC YQGLTGLHCV WCQITLHNKC 350
ASHLKPECDC GPLKDHILPP TTICPVVLQT LPTSGVSVPE ERQSTVKKEK 400
SGSQQPNKVI DKNKMQRANS VTVDGQGLQV TPVPGTHPLL VFVNPKSGGK 450
QGERIYRKFQ YLLNPRQVYS LSGNGPMPGL NFFRDVPDFR VLACGGDGTV 500
GWVLDCIEKA NVGKHPPVAI LPLGTGNDLA RCLRWGGGYE GENLMKILKD 550
IENSTEIMLD RWKFEVIPND KDEKGDPVPY SIINNYFSIG VDASIAHRFH 600
IMREKHPEKF NSRMKNKFWY FEFGTSETFS ATCKKLHESV EIECDGVQID 650
LINISLEGIA ILNIPSMHGG SNLWGESKKR RSHRRIEKKG SDKRTTVTDA 700
KELKFASQDL SDQLLEVVGL EGAMEMGQIY TGLKSAGRRL AQCSCVVIRT 750
SKSLPMQIDG EPWMQTPCTI KITHKNQAPM LMGPPPKTGL FCSLVKRTRN 800
RSKE 804
Length:804
Mass (Da):90,595
Last modified:January 11, 2001 - v2
Checksum:iCBCABD2339BFE176
GO
Isoform 2 (identifier: Q9Y6T7-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     770-773: IKIT → VSTE
     774-804: Missing.

Note: No experimental confirmation available.

Show »
Length:773
Mass (Da):87,051
Checksum:i9AF7118E642F8EB8
GO

Sequence cautioni

The sequence AAS07533.1 differs from that shown. Reason: Erroneous gene model prediction.

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti789 – 7891G → S.
Corresponds to variant rs34616903 [ dbSNP | Ensembl ].
VAR_048858

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei770 – 7734IKIT → VSTE in isoform 2.
VSP_021898
Alternative sequencei774 – 80431Missing in isoform 2.
VSP_021899Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AX032742 mRNA. Translation: CAC09945.1.
AC005039 Genomic DNA. Translation: AAS07495.1.
AC005248 Genomic DNA. Translation: AAC25525.1.
AC006150 Genomic DNA. No translation available.
AC011229 Genomic DNA. Translation: AAP21868.1.
AC073258 Genomic DNA. Translation: AAS07533.1. Sequence problems.
AC105459 Genomic DNA. Translation: AAP22362.1.
CH236948 Genomic DNA. Translation: EAL24292.1.
CH236948 Genomic DNA. Translation: EAL24293.1.
CH471073 Genomic DNA. Translation: EAW93663.1.
CH471073 Genomic DNA. Translation: EAW93664.1.
BC105005 mRNA. Translation: AAI05006.1.
AB018261 mRNA. Translation: BAA34438.1.
CCDSiCCDS47547.1. [Q9Y6T7-1]
CCDS47548.1. [Q9Y6T7-2]
RefSeqiNP_004071.1. NM_004080.2. [Q9Y6T7-1]
NP_663733.1. NM_145695.2. [Q9Y6T7-2]
XP_005249685.1. XM_005249628.1. [Q9Y6T7-1]
UniGeneiHs.567255.

Genome annotation databases

EnsembliENST00000399322; ENSP00000382260; ENSG00000136267. [Q9Y6T7-1]
ENST00000403951; ENSP00000385780; ENSG00000136267. [Q9Y6T7-1]
ENST00000406247; ENSP00000386066; ENSG00000136267. [Q9Y6T7-2]
GeneIDi1607.
KEGGihsa:1607.
UCSCiuc003ssz.3. human. [Q9Y6T7-1]
uc003sta.3. human. [Q9Y6T7-2]

Polymorphism databases

DMDMi12643960.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AX032742 mRNA. Translation: CAC09945.1 .
AC005039 Genomic DNA. Translation: AAS07495.1 .
AC005248 Genomic DNA. Translation: AAC25525.1 .
AC006150 Genomic DNA. No translation available.
AC011229 Genomic DNA. Translation: AAP21868.1 .
AC073258 Genomic DNA. Translation: AAS07533.1 . Sequence problems.
AC105459 Genomic DNA. Translation: AAP22362.1 .
CH236948 Genomic DNA. Translation: EAL24292.1 .
CH236948 Genomic DNA. Translation: EAL24293.1 .
CH471073 Genomic DNA. Translation: EAW93663.1 .
CH471073 Genomic DNA. Translation: EAW93664.1 .
BC105005 mRNA. Translation: AAI05006.1 .
AB018261 mRNA. Translation: BAA34438.1 .
CCDSi CCDS47547.1. [Q9Y6T7-1 ]
CCDS47548.1. [Q9Y6T7-2 ]
RefSeqi NP_004071.1. NM_004080.2. [Q9Y6T7-1 ]
NP_663733.1. NM_145695.2. [Q9Y6T7-2 ]
XP_005249685.1. XM_005249628.1. [Q9Y6T7-1 ]
UniGenei Hs.567255.

3D structure databases

ProteinModelPortali Q9Y6T7.
SMRi Q9Y6T7. Positions 10-222, 245-286.
ModBasei Search...

Protein-protein interaction databases

BioGridi 107977. 2 interactions.

Chemistry

DrugBanki DB00144. Phosphatidylserine.

PTM databases

PhosphoSitei Q9Y6T7.

Polymorphism databases

DMDMi 12643960.

Proteomic databases

MaxQBi Q9Y6T7.
PaxDbi Q9Y6T7.
PRIDEi Q9Y6T7.

Protocols and materials databases

DNASUi 1607.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000399322 ; ENSP00000382260 ; ENSG00000136267 . [Q9Y6T7-1 ]
ENST00000403951 ; ENSP00000385780 ; ENSG00000136267 . [Q9Y6T7-1 ]
ENST00000406247 ; ENSP00000386066 ; ENSG00000136267 . [Q9Y6T7-2 ]
GeneIDi 1607.
KEGGi hsa:1607.
UCSCi uc003ssz.3. human. [Q9Y6T7-1 ]
uc003sta.3. human. [Q9Y6T7-2 ]

Organism-specific databases

CTDi 1607.
GeneCardsi GC07M014153.
HGNCi HGNC:2850. DGKB.
MIMi 604070. gene.
neXtProti NX_Q9Y6T7.
PharmGKBi PA27311.
HUGEi Search...
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG47311.
HOVERGENi HBG051345.
InParanoidi Q9Y6T7.
KOi K00901.
OMAi CIEKANL.
OrthoDBi EOG75XGK8.
PhylomeDBi Q9Y6T7.
TreeFami TF313104.

Enzyme and pathway databases

Reactomei REACT_2202. Effects of PIP2 hydrolysis.
SABIO-RKi Q9Y6T7.

Miscellaneous databases

ChiTaRSi Dgkb. human.
GeneWikii DGKB.
GenomeRNAii 1607.
NextBioi 6600.
PROi Q9Y6T7.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q9Y6T7.
Bgeei Q9Y6T7.
Genevestigatori Q9Y6T7.

Family and domain databases

Gene3Di 1.10.238.10. 1 hit.
1.10.238.110. 1 hit.
InterProi IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR029477. DAG_kinase_typeI_N.
IPR000756. Diacylglycerol_kin_accessory.
IPR001206. Diacylglycerol_kinase_cat_dom.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
[Graphical view ]
Pfami PF00130. C1_1. 2 hits.
PF14513. DAG_kinase_N. 1 hit.
PF00609. DAGK_acc. 1 hit.
PF00781. DAGK_cat. 1 hit.
PF13499. EF-hand_7. 1 hit.
[Graphical view ]
SMARTi SM00109. C1. 2 hits.
SM00045. DAGKa. 1 hit.
SM00046. DAGKc. 1 hit.
SM00054. EFh. 2 hits.
[Graphical view ]
SUPFAMi SSF111331. SSF111331. 2 hits.
PROSITEi PS50146. DAGK. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 2 hits.
PS00479. ZF_DAG_PE_1. 2 hits.
PS50081. ZF_DAG_PE_2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The DNA sequence of human chromosome 7."
    Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L.
    , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K.
    Nature 424:157-164(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "Human chromosome 7: DNA sequence and biology."
    Scherer S.W., Cheung J., MacDonald J.R., Osborne L.R., Nakabayashi K., Herbrick J.-A., Carson A.R., Parker-Katiraee L., Skaug J., Khaja R., Zhang J., Hudek A.K., Li M., Haddad M., Duggan G.E., Fernandez B.A., Kanematsu E., Gentles S.
    , Christopoulos C.C., Choufani S., Kwasnicka D., Zheng X.H., Lai Z., Nusskern D.R., Zhang Q., Gu Z., Lu F., Zeesman S., Nowaczyk M.J., Teshima I., Chitayat D., Shuman C., Weksberg R., Zackai E.H., Grebe T.A., Cox S.R., Kirkpatrick S.J., Rahman N., Friedman J.M., Heng H.H.Q., Pelicci P.G., Lo-Coco F., Belloni E., Shaffer L.G., Pober B., Morton C.C., Gusella J.F., Bruns G.A.P., Korf B.R., Quade B.J., Ligon A.H., Ferguson H., Higgins A.W., Leach N.T., Herrick S.R., Lemyre E., Farra C.G., Kim H.-G., Summers A.M., Gripp K.W., Roberts W., Szatmari P., Winsor E.J.T., Grzeschik K.-H., Teebi A., Minassian B.A., Kere J., Armengol L., Pujana M.A., Estivill X., Wilson M.D., Koop B.F., Tosi S., Moore G.E., Boright A.P., Zlotorynski E., Kerem B., Kroisel P.M., Petek E., Oscier D.G., Mould S.J., Doehner H., Doehner K., Rommens J.M., Vincent J.B., Venter J.C., Li P.W., Mural R.J., Adams M.D., Tsui L.-C.
    Science 300:767-772(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Brain.
  6. "Prediction of the coding sequences of unidentified human genes. XI. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
    Nagase T., Ishikawa K., Suyama M., Kikuno R., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.
    DNA Res. 5:277-286(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 63-804 (ISOFORM 1).
    Tissue: Brain.

Entry informationi

Entry nameiDGKB_HUMAN
AccessioniPrimary (citable) accession number: Q9Y6T7
Secondary accession number(s): A4D116
, A4D117, A8MXU2, O75241, Q2M377, Q75MF9, Q75MU7, Q86UI5, Q86UM9, Q9UQ29
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: January 11, 2001
Last modified: September 3, 2014
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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