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Q9Y6Q5

- AP1M2_HUMAN

UniProt

Q9Y6Q5 - AP1M2_HUMAN

Protein

AP-1 complex subunit mu-2

Gene

AP1M2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 126 (01 Oct 2014)
      Sequence version 4 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the trans-Golgi network (TGN) and endosomes. The AP complexes mediate the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.

    GO - Biological processi

    1. antigen processing and presentation of exogenous peptide antigen via MHC class II Source: Reactome
    2. membrane organization Source: Reactome
    3. post-Golgi vesicle-mediated transport Source: Reactome
    4. protein targeting Source: ProtInc
    5. regulation of defense response to virus by virus Source: Reactome
    6. vesicle targeting Source: ProtInc
    7. viral process Source: Reactome

    Keywords - Biological processi

    Protein transport, Transport

    Enzyme and pathway databases

    ReactomeiREACT_11103. Nef mediated downregulation of MHC class I complex cell surface expression.
    REACT_121399. MHC class II antigen presentation.
    REACT_19287. Lysosome Vesicle Biogenesis.
    REACT_19400. Golgi Associated Vesicle Biogenesis.
    SignaLinkiQ9Y6Q5.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    AP-1 complex subunit mu-2
    Alternative name(s):
    AP-mu chain family member mu1B
    Adaptor protein complex AP-1 subunit mu-2
    Adaptor-related protein complex 1 subunit mu-2
    Clathrin assembly protein complex 1 mu-2 medium chain 2
    Golgi adaptor HA1/AP1 adaptin mu-2 subunit
    Mu-adaptin 2
    Mu1B-adaptin
    Gene namesi
    Name:AP1M2
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 19

    Organism-specific databases

    HGNCiHGNC:558. AP1M2.

    Subcellular locationi

    Golgi apparatus. Cytoplasmic vesicleclathrin-coated vesicle membrane; Peripheral membrane protein; Cytoplasmic side
    Note: Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex.

    GO - Cellular componenti

    1. clathrin adaptor complex Source: InterPro
    2. clathrin-coated vesicle membrane Source: UniProtKB-SubCell
    3. cytoplasmic vesicle membrane Source: Reactome
    4. cytosol Source: Reactome
    5. Golgi membrane Source: Reactome
    6. lysosomal membrane Source: Reactome
    7. trans-Golgi network membrane Source: Reactome

    Keywords - Cellular componenti

    Cytoplasmic vesicle, Golgi apparatus, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA24849.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 423423AP-1 complex subunit mu-2PRO_0000193772Add
    BLAST

    Post-translational modificationi

    Phosphorylation of membrane-bound AP1M1/AP1M2 increases its affinity for sorting signals.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ9Y6Q5.
    PaxDbiQ9Y6Q5.
    PRIDEiQ9Y6Q5.

    PTM databases

    PhosphoSiteiQ9Y6Q5.

    Expressioni

    Gene expression databases

    ArrayExpressiQ9Y6Q5.
    BgeeiQ9Y6Q5.
    CleanExiHS_AP1M2.
    GenevestigatoriQ9Y6Q5.

    Interactioni

    Subunit structurei

    Adaptor protein complex 1 (AP-1) is a heterotetramer composed of two large adaptins (gamma-type subunit AP1G1 and beta-type subunit AP1B1), a medium adaptin (mu-type subunit AP1M1 or AP1M2) and a small adaptin (sigma-type subunit AP1S1 or AP1S2 or AP1S3).

    Protein-protein interaction databases

    BioGridi115364. 21 interactions.
    IntActiQ9Y6Q5. 6 interactions.
    MINTiMINT-1448582.
    STRINGi9606.ENSP00000250244.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9Y6Q5.
    SMRiQ9Y6Q5. Positions 2-422.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini168 – 421254MHDPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 MHD (mu homology) domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG315786.
    HOGENOMiHOG000173247.
    HOVERGENiHBG050516.
    InParanoidiQ9Y6Q5.
    KOiK12393.
    OMAiQHEEEGL.
    PhylomeDBiQ9Y6Q5.
    TreeFamiTF300393.

    Family and domain databases

    InterProiIPR022775. AP_mu_sigma_su.
    IPR001392. Clathrin_mu.
    IPR008968. Clathrin_mu_C.
    IPR018240. Clathrin_mu_CS.
    IPR011012. Longin-like_dom.
    IPR028565. MHD.
    [Graphical view]
    PfamiPF00928. Adap_comp_sub. 1 hit.
    PF01217. Clat_adaptor_s. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005992. Clathrin_mu. 1 hit.
    PRINTSiPR00314. CLATHRINADPT.
    SUPFAMiSSF49447. SSF49447. 1 hit.
    SSF64356. SSF64356. 1 hit.
    PROSITEiPS00990. CLAT_ADAPTOR_M_1. 1 hit.
    PS00991. CLAT_ADAPTOR_M_2. 1 hit.
    PS51072. MHD. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9Y6Q5-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSASAVFILD VKGKPLISRN YKGDVAMSKI EHFMPLLVQR EEEGALAPLL    50
    SHGQVHFLWI KHSNLYLVAT TSKNANASLV YSFLYKTIEV FCEYFKELEE 100
    ESIRDNFVIV YELLDELMDF GFPQTTDSKI LQEYITQQSN KLETGKSRVP 150
    PTVTNAVSWR SEGIKYKKNE VFIDVIESVN LLVNANGSVL LSEIVGTIKL 200
    KVFLSGMPEL RLGLNDRVLF ELTGRSKNKS VELEDVKFHQ CVRLSRFDND 250
    RTISFIPPDG DFELMSYRLS TQVKPLIWIE SVIEKFSHSR VEIMVKAKGQ 300
    FKKQSVANGV EISVPVPSDA DSPRFKTSVG SAKYVPERNV VIWSIKSFPG 350
    GKEYLMRAHF GLPSVEKEEV EGRPPIGVKF EIPYFTVSGI QVRYMKIIEK 400
    SGYQALPWVR YITQSGDYQL RTS 423
    Length:423
    Mass (Da):48,108
    Last modified:January 23, 2007 - v4
    Checksum:iA3F6FDF8659DD6F7
    GO
    Isoform 2 (identifier: Q9Y6Q5-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         225-225: R → LSG

    Note: No experimental confirmation available.

    Show »
    Length:425
    Mass (Da):48,210
    Checksum:i93CA6C8D3A74ABBF
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti364 – 3641S → R in AAH05021. (PubMed:15489334)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei225 – 2251R → LSG in isoform 2. 1 PublicationVSP_000169

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF020797 mRNA. Translation: AAD25870.2.
    AK315689 mRNA. Translation: BAG38052.1.
    CH471106 Genomic DNA. Translation: EAW84124.1.
    BC003387 mRNA. Translation: AAH03387.1.
    BC003612 mRNA. Translation: AAH03612.1.
    BC005021 mRNA. Translation: AAH05021.1.
    CCDSiCCDS45964.1. [Q9Y6Q5-1]
    RefSeqiNP_005489.2. NM_005498.4. [Q9Y6Q5-1]
    XP_005259758.1. XM_005259701.1. [Q9Y6Q5-2]
    UniGeneiHs.18894.

    Genome annotation databases

    EnsembliENST00000250244; ENSP00000250244; ENSG00000129354. [Q9Y6Q5-1]
    ENST00000590923; ENSP00000465685; ENSG00000129354. [Q9Y6Q5-2]
    GeneIDi10053.
    KEGGihsa:10053.
    UCSCiuc002mpc.3. human. [Q9Y6Q5-1]
    uc002mpd.3. human. [Q9Y6Q5-2]

    Polymorphism databases

    DMDMi13123953.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF020797 mRNA. Translation: AAD25870.2 .
    AK315689 mRNA. Translation: BAG38052.1 .
    CH471106 Genomic DNA. Translation: EAW84124.1 .
    BC003387 mRNA. Translation: AAH03387.1 .
    BC003612 mRNA. Translation: AAH03612.1 .
    BC005021 mRNA. Translation: AAH05021.1 .
    CCDSi CCDS45964.1. [Q9Y6Q5-1 ]
    RefSeqi NP_005489.2. NM_005498.4. [Q9Y6Q5-1 ]
    XP_005259758.1. XM_005259701.1. [Q9Y6Q5-2 ]
    UniGenei Hs.18894.

    3D structure databases

    ProteinModelPortali Q9Y6Q5.
    SMRi Q9Y6Q5. Positions 2-422.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 115364. 21 interactions.
    IntActi Q9Y6Q5. 6 interactions.
    MINTi MINT-1448582.
    STRINGi 9606.ENSP00000250244.

    PTM databases

    PhosphoSitei Q9Y6Q5.

    Polymorphism databases

    DMDMi 13123953.

    Proteomic databases

    MaxQBi Q9Y6Q5.
    PaxDbi Q9Y6Q5.
    PRIDEi Q9Y6Q5.

    Protocols and materials databases

    DNASUi 10053.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000250244 ; ENSP00000250244 ; ENSG00000129354 . [Q9Y6Q5-1 ]
    ENST00000590923 ; ENSP00000465685 ; ENSG00000129354 . [Q9Y6Q5-2 ]
    GeneIDi 10053.
    KEGGi hsa:10053.
    UCSCi uc002mpc.3. human. [Q9Y6Q5-1 ]
    uc002mpd.3. human. [Q9Y6Q5-2 ]

    Organism-specific databases

    CTDi 10053.
    GeneCardsi GC19M010683.
    HGNCi HGNC:558. AP1M2.
    MIMi 607309. gene.
    neXtProti NX_Q9Y6Q5.
    PharmGKBi PA24849.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG315786.
    HOGENOMi HOG000173247.
    HOVERGENi HBG050516.
    InParanoidi Q9Y6Q5.
    KOi K12393.
    OMAi QHEEEGL.
    PhylomeDBi Q9Y6Q5.
    TreeFami TF300393.

    Enzyme and pathway databases

    Reactomei REACT_11103. Nef mediated downregulation of MHC class I complex cell surface expression.
    REACT_121399. MHC class II antigen presentation.
    REACT_19287. Lysosome Vesicle Biogenesis.
    REACT_19400. Golgi Associated Vesicle Biogenesis.
    SignaLinki Q9Y6Q5.

    Miscellaneous databases

    GeneWikii AP1M2.
    GenomeRNAii 10053.
    NextBioi 37981.
    PROi Q9Y6Q5.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9Y6Q5.
    Bgeei Q9Y6Q5.
    CleanExi HS_AP1M2.
    Genevestigatori Q9Y6Q5.

    Family and domain databases

    InterProi IPR022775. AP_mu_sigma_su.
    IPR001392. Clathrin_mu.
    IPR008968. Clathrin_mu_C.
    IPR018240. Clathrin_mu_CS.
    IPR011012. Longin-like_dom.
    IPR028565. MHD.
    [Graphical view ]
    Pfami PF00928. Adap_comp_sub. 1 hit.
    PF01217. Clat_adaptor_s. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005992. Clathrin_mu. 1 hit.
    PRINTSi PR00314. CLATHRINADPT.
    SUPFAMi SSF49447. SSF49447. 1 hit.
    SSF64356. SSF64356. 1 hit.
    PROSITEi PS00990. CLAT_ADAPTOR_M_1. 1 hit.
    PS00991. CLAT_ADAPTOR_M_2. 1 hit.
    PS51072. MHD. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Tissue: Placenta.
    2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Testis.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Tissue: Colon and Placenta.
    5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiAP1M2_HUMAN
    AccessioniPrimary (citable) accession number: Q9Y6Q5
    Secondary accession number(s): B2RDV5, Q9BSI8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 21, 2001
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 126 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 19
      Human chromosome 19: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3