Reviewed, UniProtKB/Swiss-Prot Q9Y6Q5 (AP1M2_HUMAN)

Names and origin

Protein names

Recommended name:
    AP-1 complex subunit mu-2
Alternative name(s):
    Adaptor-related protein complex 1 mu-2 subunit
    Adaptor protein complex AP-1 mu-2 subunit
    Golgi adaptor HA1/AP1 adaptin mu-2 subunit
    Mu-adaptin 2
    AP-mu chain family member mu1B
    Mu1B-adaptin
    Clathrin assembly protein complex 1 medium chain 2

Gene names

Name:

AP1M2

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	423 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the trans-Golgi network (TGN) and endosomes. The AP complexes mediate the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.
Subunit structure	Adaptor protein complex 1 (AP-1) is an heterotetramer composed of two large adaptins (gamma-type subunit AP1G1 and beta-type subunit AP1B1), a medium adaptin (mu-type subunit AP1M1 or AP1M2) and a small adaptin (sigma-type subunit AP1S1 or AP1S2 or AP1S3).
Subcellular location	Golgi apparatus. Cytoplasmic vesicle › clathrin-coated vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Note: Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex.
Post-translational modification	Phosphorylation of membrane-bound AP1M1/AP1M2 increases its affinity for sorting signals By similarity.
Sequence similarities	Belongs to the adaptor complexes medium subunit family. Contains 1 MHD (mu homology) domain.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Cytoplasmic vesicle Golgi apparatus Membrane
Coding sequence diversity	Alternative splicing
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein targeting Ref.1 Traceable author statement. Source: ProtInc vesicle targeting Ref.1 Traceable author statement. Source: ProtInc
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-SubCell clathrin adaptor complex Inferred from electronic annotation. Source: InterPro clathrin coated vesicle membrane Inferred from electronic annotation. Source: UniProtKB-SubCell cytosol Inferred from Experiment. Source: Reactome
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
NDRG1	Q92597	1	EBI-752259,EBI-716486

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9Y6Q5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9Y6Q5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     225-225: R → LSG

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 423	422	AP-1 complex subunit mu-2		PRO_0000193772
Regions
	Domain	165 – 422	258	MHD
Amino acid modifications
	Modified residue	2	1	N-acetylserine By similarity
Natural variations
	Alternative sequence	225	1	R → LSG in isoform 2.		VSP_000169
Experimental info
	Sequence conflict	364	1	S → R in AAH05021. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified January 23, 2007. Version 4. Checksum: A3F6FDF8659DD6F7	FASTA	423	48,108
	10 20 30 40 50 60 MSASAVFILD VKGKPLISRN YKGDVAMSKI EHFMPLLVQR EEEGALAPLL SHGQVHFLWI 70 80 90 100 110 120 KHSNLYLVAT TSKNANASLV YSFLYKTIEV FCEYFKELEE ESIRDNFVIV YELLDELMDF 130 140 150 160 170 180 GFPQTTDSKI LQEYITQQSN KLETGKSRVP PTVTNAVSWR SEGIKYKKNE VFIDVIESVN 190 200 210 220 230 240 LLVNANGSVL LSEIVGTIKL KVFLSGMPEL RLGLNDRVLF ELTGRSKNKS VELEDVKFHQ 250 260 270 280 290 300 CVRLSRFDND RTISFIPPDG DFELMSYRLS TQVKPLIWIE SVIEKFSHSR VEIMVKAKGQ 310 320 330 340 350 360 FKKQSVANGV EISVPVPSDA DSPRFKTSVG SAKYVPERNV VIWSIKSFPG GKEYLMRAHF 370 380 390 400 410 420 GLPSVEKEEV EGRPPIGVKF EIPYFTVSGI QVRYMKIIEK SGYQALPWVR YITQSGDYQL RTS
	Isoform 2. Checksum: 93CA6C8D3A74ABBF	FASTA	425	48,210
	10 20 30 40 50 60 MSASAVFILD VKGKPLISRN YKGDVAMSKI EHFMPLLVQR EEEGALAPLL SHGQVHFLWI 70 80 90 100 110 120 KHSNLYLVAT TSKNANASLV YSFLYKTIEV FCEYFKELEE ESIRDNFVIV YELLDELMDF 130 140 150 160 170 180 GFPQTTDSKI LQEYITQQSN KLETGKSRVP PTVTNAVSWR SEGIKYKKNE VFIDVIESVN 190 200 210 220 230 240 LLVNANGSVL LSEIVGTIKL KVFLSGMPEL RLGLNDRVLF ELTGLSGSKN KSVELEDVKF 250 260 270 280 290 300 HQCVRLSRFD NDRTISFIPP DGDFELMSYR LSTQVKPLIW IESVIEKFSH SRVEIMVKAK 310 320 330 340 350 360 GQFKKQSVAN GVEISVPVPS DADSPRFKTS VGSAKYVPER NVVIWSIKSF PGGKEYLMRA 370 380 390 400 410 420 HFGLPSVEKE EVEGRPPIGV KFEIPYFTVS GIQVRYMKII EKSGYQALPW VRYITQSGDY QLRTS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Mu1B, a novel adaptor medium chain expressed in polarized epithelial cells." Ohno H., Tomemori T., Nakatsu F., Okazaki Y., Aguilar R.C., Foelsch H., Mellman I., Saito T., Shirasawa T., Bonifacino J.S. FEBS Lett. 449:215-220(1999) [PubMed: 10338135] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Placenta.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Colon and Placenta.
[3]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF020797 mRNA. Translation: AAD25870.2. BC003387 mRNA. Translation: AAH03387.1. BC003612 mRNA. Translation: AAH03612.1. BC005021 mRNA. Translation: AAH05021.1.
IPI	IPI00002552. IPI00219794.
RefSeq	NP_005489.2.
UniGene	Hs.18894
3D structure databases
HSSP	HSSP built from PDB template 1H6E based on UniProtKB P20172.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9Y6Q5. 2 interactions.
Proteomic databases
PRIDE	Q9Y6Q5.
Genome annotation databases
Ensembl	ENSG00000129354. Homo sapiens. [Contig view]
GeneID	10053.
KEGG	hsa:10053.
UCSC	uc002mpc.1. human. uc002mpd.1. human.
Organism-specific databases
GeneCards	GC19M010544.
H-InvDB	HIX0014751.
HGNC	HGNC:558. AP1M2.
MIM	607309. gene.
PharmGKB	PA24849.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q9Y6Q5.
HOVERGEN	Q9Y6Q5.
OMA	Q9Y6Q5. LTGRSKN.
Enzyme and pathway databases
Reactome	REACT_11123. Membrane Trafficking. REACT_6185. HIV Infection.
Gene expression databases
ArrayExpress	Q9Y6Q5.
Bgee	Q9Y6Q5.
CleanEx	HS_AP1M2.
GermOnline	ENSG00000129354. Homo sapiens.
Family and domain databases
InterPro	IPR001392. Clathrin_mu. IPR008968. Clathrin_mu_C. IPR018240. Clathrin_mu_CS. [Graphical view]
Pfam	PF00928. Adap_comp_sub. 1 hit. [Graphical view]
PIRSF	PIRSF005992. Clathrin_mu. 1 hit.
PRINTS	PR00314. CLATHRINADPT.
PROSITE	PS00990. CLAT_ADAPTOR_M_1. 1 hit. PS00991. CLAT_ADAPTOR_M_2. 1 hit. PS51072. MHD. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	37981.
SOURCE	Search...

Entry information

Entry name

AP1M2_HUMAN

Accession

Primary (citable) accession number: Q9Y6Q5
Secondary accession number(s): Q9BSI8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2001
Last sequence update:	January 23, 2007
Last modified:	July 7, 2009
This is version 74 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)