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Q9Y6Q5 (AP1M2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 122. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
AP-1 complex subunit mu-2
Alternative name(s):
AP-mu chain family member mu1B
Adaptor protein complex AP-1 subunit mu-2
Adaptor-related protein complex 1 subunit mu-2
Clathrin assembly protein complex 1 mu-2 medium chain 2
Golgi adaptor HA1/AP1 adaptin mu-2 subunit
Mu-adaptin 2
Mu1B-adaptin
Gene names
Name:AP1M2
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length423 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the trans-Golgi network (TGN) and endosomes. The AP complexes mediate the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.

Subunit structure

Adaptor protein complex 1 (AP-1) is a heterotetramer composed of two large adaptins (gamma-type subunit AP1G1 and beta-type subunit AP1B1), a medium adaptin (mu-type subunit AP1M1 or AP1M2) and a small adaptin (sigma-type subunit AP1S1 or AP1S2 or AP1S3).

Subcellular location

Golgi apparatus. Cytoplasmic vesicleclathrin-coated vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Note: Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex.

Post-translational modification

Phosphorylation of membrane-bound AP1M1/AP1M2 increases its affinity for sorting signals By similarity.

Sequence similarities

Belongs to the adaptor complexes medium subunit family.

Contains 1 MHD (mu homology) domain.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9Y6Q5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9Y6Q5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     225-225: R → LSG
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 423423AP-1 complex subunit mu-2
PRO_0000193772

Regions

Domain168 – 421254MHD

Natural variations

Alternative sequence2251R → LSG in isoform 2.
VSP_000169

Experimental info

Sequence conflict3641S → R in AAH05021. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified January 23, 2007. Version 4.
Checksum: A3F6FDF8659DD6F7

FASTA42348,108
        10         20         30         40         50         60 
MSASAVFILD VKGKPLISRN YKGDVAMSKI EHFMPLLVQR EEEGALAPLL SHGQVHFLWI 

        70         80         90        100        110        120 
KHSNLYLVAT TSKNANASLV YSFLYKTIEV FCEYFKELEE ESIRDNFVIV YELLDELMDF 

       130        140        150        160        170        180 
GFPQTTDSKI LQEYITQQSN KLETGKSRVP PTVTNAVSWR SEGIKYKKNE VFIDVIESVN 

       190        200        210        220        230        240 
LLVNANGSVL LSEIVGTIKL KVFLSGMPEL RLGLNDRVLF ELTGRSKNKS VELEDVKFHQ 

       250        260        270        280        290        300 
CVRLSRFDND RTISFIPPDG DFELMSYRLS TQVKPLIWIE SVIEKFSHSR VEIMVKAKGQ 

       310        320        330        340        350        360 
FKKQSVANGV EISVPVPSDA DSPRFKTSVG SAKYVPERNV VIWSIKSFPG GKEYLMRAHF 

       370        380        390        400        410        420 
GLPSVEKEEV EGRPPIGVKF EIPYFTVSGI QVRYMKIIEK SGYQALPWVR YITQSGDYQL 


RTS 

« Hide

Isoform 2 [UniParc].

Checksum: 93CA6C8D3A74ABBF
Show »

FASTA42548,210

References

« Hide 'large scale' references
[1]"Mu1B, a novel adaptor medium chain expressed in polarized epithelial cells."
Ohno H., Tomemori T., Nakatsu F., Okazaki Y., Aguilar R.C., Foelsch H., Mellman I., Saito T., Shirasawa T., Bonifacino J.S.
FEBS Lett. 449:215-220(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Placenta.
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Testis.
[3]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Tissue: Colon and Placenta.
[5]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF020797 mRNA. Translation: AAD25870.2.
AK315689 mRNA. Translation: BAG38052.1.
CH471106 Genomic DNA. Translation: EAW84124.1.
BC003387 mRNA. Translation: AAH03387.1.
BC003612 mRNA. Translation: AAH03612.1.
BC005021 mRNA. Translation: AAH05021.1.
RefSeqNP_005489.2. NM_005498.4.
XP_005259758.1. XM_005259701.1.
UniGeneHs.18894.

3D structure databases

ProteinModelPortalQ9Y6Q5.
SMRQ9Y6Q5. Positions 2-422.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid115364. 21 interactions.
IntActQ9Y6Q5. 6 interactions.
MINTMINT-1448582.
STRING9606.ENSP00000250244.

PTM databases

PhosphoSiteQ9Y6Q5.

Polymorphism databases

DMDM13123953.

Proteomic databases

PaxDbQ9Y6Q5.
PRIDEQ9Y6Q5.

Protocols and materials databases

DNASU10053.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000250244; ENSP00000250244; ENSG00000129354. [Q9Y6Q5-1]
ENST00000590923; ENSP00000465685; ENSG00000129354. [Q9Y6Q5-2]
GeneID10053.
KEGGhsa:10053.
UCSCuc002mpc.3. human. [Q9Y6Q5-1]
uc002mpd.3. human. [Q9Y6Q5-2]

Organism-specific databases

CTD10053.
GeneCardsGC19M010683.
HGNCHGNC:558. AP1M2.
MIM607309. gene.
neXtProtNX_Q9Y6Q5.
PharmGKBPA24849.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG315786.
HOGENOMHOG000173247.
HOVERGENHBG050516.
InParanoidQ9Y6Q5.
KOK12393.
OMAQHEEEGL.
PhylomeDBQ9Y6Q5.
TreeFamTF300393.

Enzyme and pathway databases

ReactomeREACT_11123. Membrane Trafficking.
REACT_116125. Disease.
REACT_6900. Immune System.
SignaLinkQ9Y6Q5.

Gene expression databases

ArrayExpressQ9Y6Q5.
BgeeQ9Y6Q5.
CleanExHS_AP1M2.
GenevestigatorQ9Y6Q5.

Family and domain databases

InterProIPR022775. AP_mu_sigma_su.
IPR001392. Clathrin_mu.
IPR008968. Clathrin_mu_C.
IPR018240. Clathrin_mu_CS.
IPR011012. Longin-like_dom.
IPR028565. MHD.
[Graphical view]
PfamPF00928. Adap_comp_sub. 1 hit.
PF01217. Clat_adaptor_s. 1 hit.
[Graphical view]
PIRSFPIRSF005992. Clathrin_mu. 1 hit.
PRINTSPR00314. CLATHRINADPT.
SUPFAMSSF49447. SSF49447. 1 hit.
SSF64356. SSF64356. 1 hit.
PROSITEPS00990. CLAT_ADAPTOR_M_1. 1 hit.
PS00991. CLAT_ADAPTOR_M_2. 1 hit.
PS51072. MHD. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiAP1M2.
GenomeRNAi10053.
NextBio37981.
PROQ9Y6Q5.
SOURCESearch...

Entry information

Entry nameAP1M2_HUMAN
AccessionPrimary (citable) accession number: Q9Y6Q5
Secondary accession number(s): B2RDV5, Q9BSI8
Entry history
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: January 23, 2007
Last modified: April 16, 2014
This is version 122 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 19

Human chromosome 19: entries, gene names and cross-references to MIM