Reviewed, UniProtKB/Swiss-Prot Q9Y6M7 (S4A7_HUMAN)

Names and origin

Protein names

Recommended name:
    Sodium bicarbonate cotransporter 3
Alternative name(s):
    Sodium bicarbonate cotransporter 2
    Sodium bicarbonate cotransporter 2b
      Short name=Bicarbonate transporter
    Solute carrier family 4 member 7

Gene names

Name:	SLC4A7
Synonyms:	BT, NBC2, NBC2B, NBC3, SBC2, SLC4A6

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	1214 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Electroneutral sodium- and bicarbonate-dependent cotransporter with a Na+:HCO3- 1:1 stoichiometry. Regulates intracellular pH and may play a role in bicarbonate salvage in secretory epithelia. May also have an associated sodium channel activity. Ref.2 Ref.7
Enzyme regulation	Transporter activity is regulated by CA2/carbonic anhydrase 2, cAMP and PKA. Insensitive to stilbene derivatives. Ref.2 states it is inhibited by 5-(N-ethyl-N-isopropyl)-amiloride (EIPA).
Subunit structure	Interacts with CFTR through SLC9A3R1/EBP50. Interacts with USH1C. Forms a complex with ATP6V1B1 and SLC9A3R1/EBP50. Interacts in a pH dependent-manner with CA2/carbonic anhydrase 2. Ref.7 Ref.8 Ref.9 Ref.11
Subcellular location	Basolateral cell membrane; Multi-pass membrane protein. Apical cell membrane; Multi-pass membrane protein By similarity. Cell projection › stereocilium By similarity. Note: Also described at the apical cell membrane. Localizes to the stereocilia of cochlear outer hair cells and to the lateral membrane of cochlear inner hair cells By similarity.
Tissue specificity	Highly expressed in testis and spleen. Also expressed in retina, colon, small intestine, ovary, thymus, prostate, muscle, heart and kidney. Isoform 1 is expressed in skeletal muscle and heart muscle. Ref.1
Domain	The PDZ-binding motif mediates interaction with the CFTR, SLC9A3R1/EBP50 complex and probably with USH1C. Ref.7
Post-translational modification	N-glycosylated By similarity.
Sequence similarities	Belongs to the anion exchanger (TC 2.A.31) family. [View classification]
biophysicochemical properties	Kinetic parameters: KM=24 mM for external sodium

Ontologies

Keywords
Biological process	Ion transport Sodium transport Symport Transport
Cellular component	Cell membrane Cell projection Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Transmembrane
Ligand	Sodium
PTM	Glycoprotein Phosphoprotein
Gene Ontology (GO)
Biological process	bicarbonate transport Ref.2 Non-traceable author statement. Source: UniProtKB sodium ion transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	apical plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell basolateral plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Ref.2 Non-traceable author statement. Source: UniProtKB stereocilium Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	inorganic anion exchanger activity Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct sodium ion binding Inferred from electronic annotation. Source: UniProtKB-KW sodium:bicarbonate symporter activity Ref.2 Non-traceable author statement. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
YWHAB	P31946	1	EBI-1044546,EBI-359815

Alternative products

This entry describes 5 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9Y6M7-1)

Also known as: mNBC3; NBCn1-A;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9Y6M7-2)

The sequence of this isoform differs from the canonical sequence as follows:
     251-374: Missing.

Isoform 3 (identifier: Q9Y6M7-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-90: Missing.
     91-118: SQRVQFILGTEDDDEEHIPHDLFTEMDE → MAVTQFIHFREEIMGNMFFIIIFSTKDK
     251-374: Missing.
     1189-1214: PDKPVSVKISFEDEPRKKYVDAETSL → GDPSIGNISDEMAKTAQWKALSMNTENAKVTRSNMSPDKPVSVK

Isoform 4 (identifier: Q9Y6M7-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-90: Missing.
     91-118: SQRVQFILGTEDDDEEHIPHDLFTEMDE → MAVTQFIHFREEIMGNMFFIIIFSTKDK
     251-374: Missing.

Isoform 5 (identifier: Q9Y6M7-5)

The sequence of this isoform differs from the canonical sequence as follows:
     1-450: Missing.
     451-465: PAVLLTGLTEVPVPT → MEVVEAEKIVLLTSA
     1188-1188: S → SVDPSIVNISDEMAKTAQWKALSMNSENAKVTRSNMS

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1214	1214	Sodium bicarbonate cotransporter 3		PRO_0000079233
Regions
	Topological domain	1 – 608	608	Extracellular Potential
	Transmembrane	609 – 629	21	Potential
	Topological domain	630 – 637	8	Cytoplasmic Potential
	Transmembrane	638 – 658	21	Potential
	Topological domain	659 – 695	37	Extracellular Potential
	Transmembrane	696 – 716	21	Potential
	Topological domain	717 – 725	9	Cytoplasmic Potential
	Transmembrane	726 – 746	21	Potential
	Topological domain	747 – 817	71	Extracellular Potential
	Transmembrane	818 – 838	21	Potential
	Topological domain	839 – 861	23	Cytoplasmic Potential
	Transmembrane	862 – 882	21	Potential
	Topological domain	883 – 908	26	Extracellular Potential
	Transmembrane	909 – 929	21	Potential
	Topological domain	930 – 954	25	Cytoplasmic Potential
	Transmembrane	955 – 975	21	Potential
	Topological domain	976 – 1011	36	Extracellular Potential
	Transmembrane	1012 – 1032	21	Potential
	Topological domain	1033 – 1034	2	Cytoplasmic Potential
	Transmembrane	1035 – 1055	21	Potential
	Topological domain	1056 – 1092	37	Extracellular Potential
	Transmembrane	1093 – 1113	21	Potential
	Topological domain	1114 – 1214	101	Cytoplasmic Potential
	Region	1134 – 1136	3	CA2-binding
	Motif	1211 – 1214	4	PDZ-binding
Amino acid modifications
	Modified residue	233	1	Phosphoserine By similarity
	Modified residue	379	1	Phosphoserine By similarity
	Modified residue	382	1	Phosphoserine Ref.13
	Modified residue	403	1	Phosphoserine Ref.13
	Modified residue	407	1	Phosphoserine Ref.13
	Modified residue	1167	1	Phosphothreonine Ref.13 Ref.12
	Modified residue	1176	1	Phosphoserine Ref.13 Ref.12
	Modified residue	1188	1	Phosphoserine Ref.13
	Modified residue	1194	1	Phosphoserine Ref.13
	Modified residue	1213	1	Phosphoserine Ref.13 Ref.10
	Glycosylation	171	1	N-linked (GlcNAc...) Potential
	Glycosylation	269	1	N-linked (GlcNAc...) Potential
	Glycosylation	398	1	N-linked (GlcNAc...) Potential
	Glycosylation	406	1	N-linked (GlcNAc...) Potential
	Glycosylation	786	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	1 – 450	450	Missing in isoform 5.		VSP_017159
	Alternative sequence	1 – 90	90	Missing in isoform 3 and isoform 4.		VSP_017160
	Alternative sequence	91 – 118	28	SQRVQ…TEMDE → MAVTQFIHFREEIMGNMFFI IIFSTKDK in isoform 3 and isoform 4.		VSP_017161
	Alternative sequence	251 – 374	124	Missing in isoform 2, isoform 3 and isoform 4.		VSP_017162
	Alternative sequence	451 – 465	15	PAVLL…VPVPT → MEVVEAEKIVLLTSA in isoform 5.		VSP_017163
	Alternative sequence	1188	1	S → SVDPSIVNISDEMAKTAQWK ALSMNSENAKVTRSNMS in isoform 5.		VSP_017164
	Alternative sequence	1189 – 1214	26	PDKPV…AETSL → GDPSIGNISDEMAKTAQWKA LSMNTENAKVTRSNMSPDKP VSVK in isoform 3.		VSP_017165
	Natural variant	326	1	E → K: dbSNP rs3755652.		VAR_055317
Experimental info
	Mutagenesis	1135 – 1136	2	DD → NN: Loss of interaction with CA2. Loss of regulation by CA2.
	Mutagenesis	1163 – 1165	3	DDD → NNN: No effect on interaction with CA2. No effect on regulation by CA2. Ref.9
	Mutagenesis	1214	1	L → G: Loss of interaction with ATP6V1B1. Ref.8

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (mNBC3) (NBCn1-A) [UniParc]. Last modified May 5, 2009. Version 2. Checksum: 7B4ADA1E3F26813C	FASTA	1,214	136,044
	10 20 30 40 50 60 MERFRLEKKL PGPDEEAVVD LGKTSSTVNT KFEKEELESH RAVYIGVHVP FSKESRRRHR 70 80 90 100 110 120 HRGHKHHHRR RKDKESDKED GRESPSYDTP SQRVQFILGT EDDDEEHIPH DLFTEMDELC 130 140 150 160 170 180 YRDGEEYEWK ETARWLKFEE DVEDGGDRWS KPYVATLSLH SLFELRSCIL NGTVMLDMRA 190 200 210 220 230 240 STLDEIADMV LDNMIASGQL DESIRENVRE ALLKRHHHQN EKRFTSRIPL VRSFADIGKK 250 260 270 280 290 300 HSDPHLLERN GEGLSASRHS LRTGLSASNL SLRGESPLSL LLGHLLPSSR AGTPAGSRCT 310 320 330 340 350 360 TPVPTPQNSP PSSPSISRLT SRSSQESQRQ APELLVSPAS DDIPTVVIHP PEEDLEAALK 370 380 390 400 410 420 GEEQKNEENV DLTPGILASP QSAPGNLDNS KSGEIKGNGS GGSRENSTVD FSKVDMNFMR 430 440 450 460 470 480 KIPTGAEASN VLVGEVDFLE RPIIAFVRLA PAVLLTGLTE VPVPTRFLFL LLGPAGKAPQ 490 500 510 520 530 540 YHEIGRSIAT LMTDEIFHDV AYKAKDRNDL LSGIDEFLDQ VTVLPPGEWD PSIRIEPPKS 550 560 570 580 590 600 VPSQEKRKIP VFHNGSTPTL GETPKEAAHH AGPELQRTGR LFGGLILDIK RKAPFFLSDF 610 620 630 640 650 660 KDALSLQCLA SILFLYCACM SPVITFGGLL GEATEGRISA IESLFGASLT GIAYSLFAGQ 670 680 690 700 710 720 PLTILGSTGP VLVFEKILYK FCRDYQLSYL SLRTSIGLWT SFLCIVLVAT DASSLVCYIT 730 740 750 760 770 780 RFTEEAFAAL ICIIFIYEAL EKLFDLGETY AFNMHNNLDK LTSYSCVCTE PPNPSNETLA 790 800 810 820 830 840 QWKKDNITAH NISWRNLTVS ECKKLRGVFL GSACGHHGPY IPDVLFWCVI LFFTTFFLSS 850 860 870 880 890 900 FLKQFKTKRY FPTKVRSTIS DFAVFLTIVI MVTIDYLVGV PSPKLHVPEK FEPTHPERGW 910 920 930 940 950 960 IISPLGDNPW WTLLIAAIPA LLCTILIFMD QQITAVIINR KEHKLKKGAG YHLDLLMVGV 970 980 990 1000 1010 1020 MLGVCSVMGL PWFVAATVLS ISHVNSLKVE SECSAPGEQP KFLGIREQRV TGLMIFILMG 1030 1040 1050 1060 1070 1080 LSVFMTSVLK FIPMPVLYGV FLYMGVSSLK GIQLFDRIKL FGMPAKHQPD LIYLRYVPLW 1090 1100 1110 1120 1130 1140 KVHIFTVIQL TCLVLLWVIK VSAAAVVFPM MVLALVFVRK LMDLCFTKRE LSWLDDLMPE 1150 1160 1170 1180 1190 1200 SKKKKEDDKK KKEKEEAERM LQDDDDTVHL PFEGGSLLQI PVKALKYSPD KPVSVKISFE 1210 DEPRKKYVDA ETSL
	Isoform 2. Checksum: 541839CC9B565896	FASTA	1,090	123,081
	10 20 30 40 50 60 MERFRLEKKL PGPDEEAVVD LGKTSSTVNT KFEKEELESH RAVYIGVHVP FSKESRRRHR 70 80 90 100 110 120 HRGHKHHHRR RKDKESDKED GRESPSYDTP SQRVQFILGT EDDDEEHIPH DLFTEMDELC 130 140 150 160 170 180 YRDGEEYEWK ETARWLKFEE DVEDGGDRWS KPYVATLSLH SLFELRSCIL NGTVMLDMRA 190 200 210 220 230 240 STLDEIADMV LDNMIASGQL DESIRENVRE ALLKRHHHQN EKRFTSRIPL VRSFADIGKK 250 260 270 280 290 300 HSDPHLLERN GILASPQSAP GNLDNSKSGE IKGNGSGGSR ENSTVDFSKV DMNFMRKIPT 310 320 330 340 350 360 GAEASNVLVG EVDFLERPII AFVRLAPAVL LTGLTEVPVP TRFLFLLLGP AGKAPQYHEI 370 380 390 400 410 420 GRSIATLMTD EIFHDVAYKA KDRNDLLSGI DEFLDQVTVL PPGEWDPSIR IEPPKSVPSQ 430 440 450 460 470 480 EKRKIPVFHN GSTPTLGETP KEAAHHAGPE LQRTGRLFGG LILDIKRKAP FFLSDFKDAL 490 500 510 520 530 540 SLQCLASILF LYCACMSPVI TFGGLLGEAT EGRISAIESL FGASLTGIAY SLFAGQPLTI 550 560 570 580 590 600 LGSTGPVLVF EKILYKFCRD YQLSYLSLRT SIGLWTSFLC IVLVATDASS LVCYITRFTE 610 620 630 640 650 660 EAFAALICII FIYEALEKLF DLGETYAFNM HNNLDKLTSY SCVCTEPPNP SNETLAQWKK 670 680 690 700 710 720 DNITAHNISW RNLTVSECKK LRGVFLGSAC GHHGPYIPDV LFWCVILFFT TFFLSSFLKQ 730 740 750 760 770 780 FKTKRYFPTK VRSTISDFAV FLTIVIMVTI DYLVGVPSPK LHVPEKFEPT HPERGWIISP 790 800 810 820 830 840 LGDNPWWTLL IAAIPALLCT ILIFMDQQIT AVIINRKEHK LKKGAGYHLD LLMVGVMLGV 850 860 870 880 890 900 CSVMGLPWFV AATVLSISHV NSLKVESECS APGEQPKFLG IREQRVTGLM IFILMGLSVF 910 920 930 940 950 960 MTSVLKFIPM PVLYGVFLYM GVSSLKGIQL FDRIKLFGMP AKHQPDLIYL RYVPLWKVHI 970 980 990 1000 1010 1020 FTVIQLTCLV LLWVIKVSAA AVVFPMMVLA LVFVRKLMDL CFTKRELSWL DDLMPESKKK 1030 1040 1050 1060 1070 1080 KEDDKKKKEK EEAERMLQDD DDTVHLPFEG GSLLQIPVKA LKYSPDKPVS VKISFEDEPR 1090 KKYVDAETSL
	Isoform 3. Checksum: A37799D93A1E30BC	FASTA	1,018	114,205
	10 20 30 40 50 60 MAVTQFIHFR EEIMGNMFFI IIFSTKDKLC YRDGEEYEWK ETARWLKFEE DVEDGGDRWS 70 80 90 100 110 120 KPYVATLSLH SLFELRSCIL NGTVMLDMRA STLDEIADMV LDNMIASGQL DESIRENVRE 130 140 150 160 170 180 ALLKRHHHQN EKRFTSRIPL VRSFADIGKK HSDPHLLERN GILASPQSAP GNLDNSKSGE 190 200 210 220 230 240 IKGNGSGGSR ENSTVDFSKV DMNFMRKIPT GAEASNVLVG EVDFLERPII AFVRLAPAVL 250 260 270 280 290 300 LTGLTEVPVP TRFLFLLLGP AGKAPQYHEI GRSIATLMTD EIFHDVAYKA KDRNDLLSGI 310 320 330 340 350 360 DEFLDQVTVL PPGEWDPSIR IEPPKSVPSQ EKRKIPVFHN GSTPTLGETP KEAAHHAGPE 370 380 390 400 410 420 LQRTGRLFGG LILDIKRKAP FFLSDFKDAL SLQCLASILF LYCACMSPVI TFGGLLGEAT 430 440 450 460 470 480 EGRISAIESL FGASLTGIAY SLFAGQPLTI LGSTGPVLVF EKILYKFCRD YQLSYLSLRT 490 500 510 520 530 540 SIGLWTSFLC IVLVATDASS LVCYITRFTE EAFAALICII FIYEALEKLF DLGETYAFNM 550 560 570 580 590 600 HNNLDKLTSY SCVCTEPPNP SNETLAQWKK DNITAHNISW RNLTVSECKK LRGVFLGSAC 610 620 630 640 650 660 GHHGPYIPDV LFWCVILFFT TFFLSSFLKQ FKTKRYFPTK VRSTISDFAV FLTIVIMVTI 670 680 690 700 710 720 DYLVGVPSPK LHVPEKFEPT HPERGWIISP LGDNPWWTLL IAAIPALLCT ILIFMDQQIT 730 740 750 760 770 780 AVIINRKEHK LKKGAGYHLD LLMVGVMLGV CSVMGLPWFV AATVLSISHV NSLKVESECS 790 800 810 820 830 840 APGEQPKFLG IREQRVTGLM IFILMGLSVF MTSVLKFIPM PVLYGVFLYM GVSSLKGIQL 850 860 870 880 890 900 FDRIKLFGMP AKHQPDLIYL RYVPLWKVHI FTVIQLTCLV LLWVIKVSAA AVVFPMMVLA 910 920 930 940 950 960 LVFVRKLMDL CFTKRELSWL DDLMPESKKK KEDDKKKKEK EEAERMLQDD DDTVHLPFEG 970 980 990 1000 1010 GSLLQIPVKA LKYSGDPSIG NISDEMAKTA QWKALSMNTE NAKVTRSNMS PDKPVSVK
	Isoform 4. Checksum: 50FAA40A31468223	FASTA	1,000	112,448
	10 20 30 40 50 60 MAVTQFIHFR EEIMGNMFFI IIFSTKDKLC YRDGEEYEWK ETARWLKFEE DVEDGGDRWS 70 80 90 100 110 120 KPYVATLSLH SLFELRSCIL NGTVMLDMRA STLDEIADMV LDNMIASGQL DESIRENVRE 130 140 150 160 170 180 ALLKRHHHQN EKRFTSRIPL VRSFADIGKK HSDPHLLERN GILASPQSAP GNLDNSKSGE 190 200 210 220 230 240 IKGNGSGGSR ENSTVDFSKV DMNFMRKIPT GAEASNVLVG EVDFLERPII AFVRLAPAVL 250 260 270 280 290 300 LTGLTEVPVP TRFLFLLLGP AGKAPQYHEI GRSIATLMTD EIFHDVAYKA KDRNDLLSGI 310 320 330 340 350 360 DEFLDQVTVL PPGEWDPSIR IEPPKSVPSQ EKRKIPVFHN GSTPTLGETP KEAAHHAGPE 370 380 390 400 410 420 LQRTGRLFGG LILDIKRKAP FFLSDFKDAL SLQCLASILF LYCACMSPVI TFGGLLGEAT 430 440 450 460 470 480 EGRISAIESL FGASLTGIAY SLFAGQPLTI LGSTGPVLVF EKILYKFCRD YQLSYLSLRT 490 500 510 520 530 540 SIGLWTSFLC IVLVATDASS LVCYITRFTE EAFAALICII FIYEALEKLF DLGETYAFNM 550 560 570 580 590 600 HNNLDKLTSY SCVCTEPPNP SNETLAQWKK DNITAHNISW RNLTVSECKK LRGVFLGSAC 610 620 630 640 650 660 GHHGPYIPDV LFWCVILFFT TFFLSSFLKQ FKTKRYFPTK VRSTISDFAV FLTIVIMVTI 670 680 690 700 710 720 DYLVGVPSPK LHVPEKFEPT HPERGWIISP LGDNPWWTLL IAAIPALLCT ILIFMDQQIT 730 740 750 760 770 780 AVIINRKEHK LKKGAGYHLD LLMVGVMLGV CSVMGLPWFV AATVLSISHV NSLKVESECS 790 800 810 820 830 840 APGEQPKFLG IREQRVTGLM IFILMGLSVF MTSVLKFIPM PVLYGVFLYM GVSSLKGIQL 850 860 870 880 890 900 FDRIKLFGMP AKHQPDLIYL RYVPLWKVHI FTVIQLTCLV LLWVIKVSAA AVVFPMMVLA 910 920 930 940 950 960 LVFVRKLMDL CFTKRELSWL DDLMPESKKK KEDDKKKKEK EEAERMLQDD DDTVHLPFEG 970 980 990 1000 GSLLQIPVKA LKYSPDKPVS VKISFEDEPR KKYVDAETSL
	Isoform 5. Checksum: CCFB5D3479BF5870	FASTA	800	89,731
	10 20 30 40 50 60 MEVVEAEKIV LLTSARFLFL LLGPAGKAPQ YHEIGRSIAT LMTDEIFHDV AYKAKDRNDL 70 80 90 100 110 120 LSGIDEFLDQ VTVLPPGEWD PSIRIEPPKS VPSQEKRKIP VFHNGSTPTL GETPKEAAHH 130 140 150 160 170 180 AGPELQRTGR LFGGLILDIK RKAPFFLSDF KDALSLQCLA SILFLYCACM SPVITFGGLL 190 200 210 220 230 240 GEATEGRISA IESLFGASLT GIAYSLFAGQ PLTILGSTGP VLVFEKILYK FCRDYQLSYL 250 260 270 280 290 300 SLRTSIGLWT SFLCIVLVAT DASSLVCYIT RFTEEAFAAL ICIIFIYEAL EKLFDLGETY 310 320 330 340 350 360 AFNMHNNLDK LTSYSCVCTE PPNPSNETLA QWKKDNITAH NISWRNLTVS ECKKLRGVFL 370 380 390 400 410 420 GSACGHHGPY IPDVLFWCVI LFFTTFFLSS FLKQFKTKRY FPTKVRSTIS DFAVFLTIVI 430 440 450 460 470 480 MVTIDYLVGV PSPKLHVPEK FEPTHPERGW IISPLGDNPW WTLLIAAIPA LLCTILIFMD 490 500 510 520 530 540 QQITAVIINR KEHKLKKGAG YHLDLLMVGV MLGVCSVMGL PWFVAATVLS ISHVNSLKVE 550 560 570 580 590 600 SECSAPGEQP KFLGIREQRV TGLMIFILMG LSVFMTSVLK FIPMPVLYGV FLYMGVSSLK 610 620 630 640 650 660 GIQLFDRIKL FGMPAKHQPD LIYLRYVPLW KVHIFTVIQL TCLVLLWVIK VSAAAVVFPM 670 680 690 700 710 720 MVLALVFVRK LMDLCFTKRE LSWLDDLMPE SKKKKEDDKK KKEKEEAERM LQDDDDTVHL 730 740 750 760 770 780 PFEGGSLLQI PVKALKYSVD PSIVNISDEM AKTAQWKALS MNSENAKVTR SNMSPDKPVS 790 800 VKISFEDEPR KKYVDAETSL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of a new sodium bicarbonate cotransporter cDNA from human retina." Ishibashi K., Sasaki S., Marumo F. Biochem. Biophys. Res. Commun. 246:535-538(1998) [PubMed: 9610397] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), TISSUE SPECIFICITY. Tissue: Retina.
[2]	"Cloning, tissue distribution, genomic organization, and functional characterization of NBC3, a new member of the sodium bicarbonate cotransporter family." Pushkin A., Abuladze N., Lee I., Newman D., Hwang J., Kurtz I. J. Biol. Chem. 274:16569-16575(1999) [PubMed: 10347222] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, REGULATION, VARIANT LYS-326. Tissue: Kidney and Skeletal muscle.
[3]	"Cloning of a HCO3 transporter, NT2-NBC, from human brain, similar to both the Anion exchangers (AEs) and the Na/Bicarbonate Cotransporters (NBCs)." Romero M.F. Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 4). Tissue: Neuroepithelium.
[4]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Blocker H., Heubner D., Hoerlein A., Michel G., Wedler H., Kohrer K., Ottenwalder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 5). Tissue: Cervix.
[5]	"The DNA sequence, annotation and analysis of human chromosome 3." Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J. , Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P., Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H., Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J., Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W., Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B., Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H., Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J., Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X., Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R., Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A. Nature 440:1194-1198(2006) [PubMed: 16641997] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The cystic fibrosis transmembrane conductance regulator interacts with and regulates the activity of the HCO3- salvage transporter human Na+-HCO3-cotransport isoform 3." Park M., Ko S.B.H., Choi J.Y., Muallem G., Thomas P.J., Pushkin A., Lee M.-S., Kim J.Y., Lee M.G., Muallem S., Kurtz I. J. Biol. Chem. 277:50503-50509(2002) [PubMed: 12403779] [Abstract] Cited for: FUNCTION, INTERACTION WITH CFTR AND SLC9A3R1, DOMAIN.
[8]	"The COOH termini of NBC3 and the 56-kDa H+-ATPase subunit are PDZ motifs involved in their interaction." Pushkin A., Abuladze N., Newman D., Muronets V., Sassani P., Tatishchev S., Kurtz I. Am. J. Physiol. 284:C667-C673(2003) [PubMed: 12444018] [Abstract] Cited for: INTERACTION WITH ATP6V1B1 AND SLC9A3R1, MUTAGENESIS OF LEU-1214.
[9]	"Regulation of the human NBC3 Na+/HCO3- cotransporter by carbonic anhydrase II and PKA." Loiselle F.B., Morgan P.E., Alvarez B.V., Casey J.R. Am. J. Physiol. 286:C1423-C1433(2004) [PubMed: 14736710] [Abstract] Cited for: INTERACTION WITH CA2, REGULATION, MUTAGENESIS OF 1135-ASP-ASP-1136 AND 1163-ASP--ASP-1165.
[10]	"Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity chromatography and tandem mass spectrometry." Brill L.M., Salomon A.R., Ficarro S.B., Mukherji M., Stettler-Gill M., Peters E.C. Anal. Chem. 76:2763-2772(2004) [PubMed: 15144186] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1213, MASS SPECTROMETRY. Tissue: T-cell.
[11]	"Scaffold protein harmonin (USH1C) provides molecular links between Usher syndrome type 1 and type 2." Reiners J., van Wijk E., Maerker T., Zimmermann U., Juergens K., te Brinke H., Overlack N., Roepman R., Knipper M., Kremer H., Wolfrum U. Hum. Mol. Genet. 14:3933-3943(2005) [PubMed: 16301216] [Abstract] Cited for: INTERACTION WITH USH1C.
[12]	"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III J. Proteome Res. 7:1346-1351(2008) [PubMed: 18220336] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-1167 AND SER-1176, MASS SPECTROMETRY.
[13]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-382; SER-403; SER-407; THR-1167; SER-1176; SER-1188; SER-1194 AND SER-1213, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB012130 mRNA. Translation: BAA25898.1. AF047033 mRNA. Translation: AAD38322.1. AF089726 mRNA. Translation: AAG16773.1. AF053755 mRNA. Translation: AAF21720.1. CR627428 mRNA. Translation: CAH10515.1. AC099535 Genomic DNA. No translation available. CH471055 Genomic DNA. Translation: EAW64382.1.
IPI	IPI00021058. IPI00328342. IPI00387156. IPI00719704. IPI00719757.
PIR	JE0160.
RefSeq	NP_003606.3.
UniGene	Hs.250072
3D structure databases
HSSP	HSSP built from PDB template 1BZK based on UniProtKB P02730.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9Y6M7. 1 interaction.
PTM databases
PhosphoSite	Q9Y6M7.
Proteomic databases
PRIDE	Q9Y6M7.
Genome annotation databases
Ensembl	ENSG00000033867. Homo sapiens. [Contig view]
GeneID	9497.
Organism-specific databases
GeneCards	GC03M027389.
H-InvDB	HIX0003134.
HGNC	HGNC:11033. SLC4A7.
MIM	603353. gene.
PharmGKB	PA35899.
GenAtlas	Search...
Phylogenomic databases
HOVERGEN	Q9Y6M7.
Gene expression databases
ArrayExpress	Q9Y6M7.
Bgee	Q9Y6M7.
CleanEx	HS_SLC4A7.
GermOnline	ENSG00000033867. Homo sapiens.
Family and domain databases
InterPro	IPR018241. Anion_exchange_CS. IPR011531. HCO3_transpt_C. IPR013769. HCO3_transpt_cyt. IPR003020. HCO3_transpt_euk. [Graphical view]
Gene3D	G3DSA:3.40.1100.10. HCO3_transpt_cyt. 1 hit.
PANTHER	PTHR11453. HCO3_transpt_euk. 1 hit.
Pfam	PF07565. Band_3_cyto. 1 hit. PF00955. HCO3_cotransp. 1 hit. [Graphical view]
PRINTS	PR01231. HCO3TRNSPORT.
TIGRFAMs	TIGR00834. ae. 1 hit.
PROSITE	PS00219. ANION_EXCHANGER_1. False negative. PS00220. ANION_EXCHANGER_2. False negative. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

S4A7_HUMAN

Accession

Primary (citable) accession number: Q9Y6M7
Secondary accession number(s): A6NIA8 , O60350, Q6AHZ9, Q9HC88, Q9UIB9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 7, 2006
Last sequence update:	May 5, 2009
Last modified:	June 16, 2009
This is version 57 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)