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Protein

Casein kinase I isoform gamma-3

Gene

CSNK1G3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Serine/threonine-protein kinase. Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Regulates fast synaptic transmission mediated by glutamate (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Inhibited by triazolodiamine 1, 5-amino-3-{[4-(aminosulfonyl)phenyl]amino}-N-(2,6-difluorophenyl)-1H-1,2,4-triazole-1-carbothioamide, (S)-propane-1,2-diol, 2-({6-[(3-chlorophenyl)amino]-9-isopropyl-9H-purin-2-yl}amino)-3-methylbutan-1-ol, N2-[(1R,2S)-2-aminocyclohexyl]-N6-(3-chlorophenyl)-9-ethyl-9H-purine-2,6-diamine and [4-amino-2-(3-chloroanilino)-1,3-thiazol-5-yl](4-fluorophenyl)methanone.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei72ATPPROSITE-ProRule annotation1
Active sitei162Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi49 – 57ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • protein kinase activity Source: ProtInc
  • protein serine/threonine kinase activity Source: ParkinsonsUK-UCL

GO - Biological processi

  • cellular protein modification process Source: ProtInc
  • endocytosis Source: GO_Central
  • peptidyl-serine phosphorylation Source: ParkinsonsUK-UCL
  • regulation of cell shape Source: GO_Central
  • signal transduction Source: ProtInc
  • Wnt signaling pathway Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Wnt signaling pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciZFISH:HS07721-MONOMER.
BRENDAi2.7.11.1. 2681.
SignaLinkiQ9Y6M4.

Names & Taxonomyi

Protein namesi
Recommended name:
Casein kinase I isoform gamma-3 (EC:2.7.11.1)
Short name:
CKI-gamma 3
Gene namesi
Name:CSNK1G3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

HGNCiHGNC:2456. CSNK1G3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

DisGeNETi1456.
OpenTargetsiENSG00000151292.
PharmGKBiPA26956.

Chemistry databases

ChEMBLiCHEMBL5084.
GuidetoPHARMACOLOGYi2001.

Polymorphism and mutation databases

DMDMi47117932.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001928461 – 447Casein kinase I isoform gamma-3Add BLAST447

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei413PhosphoserineCombined sources1
Isoform 6 (identifier: Q9Y6M4-6)
Modified residuei254PhosphoserineCombined sources1
Isoform 5 (identifier: Q9Y6M4-5)
Modified residuei291PhosphoserineCombined sources1
Isoform 3 (identifier: Q9Y6M4-3)
Modified residuei366PhosphoserineCombined sources1
Isoform 4 (identifier: Q9Y6M4-4)
Modified residuei367PhosphoserineCombined sources1

Post-translational modificationi

Autophosphorylated.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9Y6M4.
PaxDbiQ9Y6M4.
PeptideAtlasiQ9Y6M4.
PRIDEiQ9Y6M4.

PTM databases

iPTMnetiQ9Y6M4.
PhosphoSitePlusiQ9Y6M4.
SwissPalmiQ9Y6M4.

Expressioni

Gene expression databases

BgeeiENSG00000151292.
CleanExiHS_CSNK1G3.
ExpressionAtlasiQ9Y6M4. baseline and differential.
GenevisibleiQ9Y6M4. HS.

Organism-specific databases

HPAiHPA027010.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

BioGridi107840. 36 interactors.
IntActiQ9Y6M4. 6 interactors.
STRINGi9606.ENSP00000353904.

Chemistry databases

BindingDBiQ9Y6M4.

Structurei

Secondary structure

1447
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni40 – 42Combined sources3
Beta strandi43 – 48Combined sources6
Beta strandi50 – 52Combined sources3
Beta strandi56 – 62Combined sources7
Turni63 – 66Combined sources4
Beta strandi67 – 75Combined sources9
Helixi83 – 93Combined sources11
Beta strandi102 – 108Combined sources7
Beta strandi111 – 117Combined sources7
Helixi123 – 129Combined sources7
Turni130 – 132Combined sources3
Helixi136 – 155Combined sources20
Helixi165 – 167Combined sources3
Beta strandi168 – 170Combined sources3
Helixi173 – 175Combined sources3
Turni176 – 179Combined sources4
Beta strandi180 – 183Combined sources4
Beta strandi190 – 193Combined sources4
Turni195 – 197Combined sources3
Beta strandi198 – 200Combined sources3
Turni213 – 215Combined sources3
Helixi218 – 221Combined sources4
Helixi228 – 244Combined sources17
Turni248 – 251Combined sources4
Helixi257 – 270Combined sources14
Helixi273 – 276Combined sources4
Turni277 – 279Combined sources3
Helixi281 – 292Combined sources12
Helixi301 – 314Combined sources14
Turni324 – 327Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2CHLX-ray1.95A35-362[»]
2IZRX-ray1.30A35-362[»]
2IZSX-ray1.95A35-362[»]
2IZTX-ray2.00A35-362[»]
2IZUX-ray1.85A35-362[»]
4G16X-ray2.30A34-362[»]
4G17X-ray2.10A34-362[»]
4HGLX-ray2.40A34-362[»]
4HGSX-ray2.40A34-362[»]
ProteinModelPortaliQ9Y6M4.
SMRiQ9Y6M4.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9Y6M4.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini43 – 313Protein kinasePROSITE-ProRule annotationAdd BLAST271

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni117 – 125Inhibitors binding9

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1165. Eukaryota.
ENOG410XNXP. LUCA.
GeneTreeiENSGT00760000119040.
HOGENOMiHOG000182054.
HOVERGENiHBG000176.
InParanoidiQ9Y6M4.
KOiK08958.
OMAiNREAHPH.
OrthoDBiEOG091G0F3Z.
PhylomeDBiQ9Y6M4.
TreeFamiTF313349.

Family and domain databases

InterProiIPR022247. Casein_kinase-1_gamma_C.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF12605. CK1gamma_C. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (6)i

Sequence statusi: Complete.

This entry describes 6 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9Y6M4-1) [UniParc]FASTAAdd to basket
Also known as: 3

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MENKKKDKDK SDDRMARPSG RSGHNTRGTG SSSSGVLMVG PNFRVGKKIG
60 70 80 90 100
CGNFGELRLG KNLYTNEYVA IKLEPMKSRA PQLHLEYRFY KQLGSGDGIP
110 120 130 140 150
QVYYFGPCGK YNAMVLELLG PSLEDLFDLC DRTFSLKTVL MIAIQLISRM
160 170 180 190 200
EYVHSKNLIY RDVKPENFLI GRPGNKTQQV IHIIDFGLAK EYIDPETKKH
210 220 230 240 250
IPYREHKSLT GTARYMSINT HLGKEQSRRD DLEALGHMFM YFLRGSLPWQ
260 270 280 290 300
GLKADTLKER YQKIGDTKRA TPIEVLCENF PEMATYLRYV RRLDFFEKPD
310 320 330 340 350
YDYLRKLFTD LFDRKGYMFD YEYDWIGKQL PTPVGAVQQD PALSSNREAH
360 370 380 390 400
QHRDKMQQSK NQSADHRAAW DSQQANPHHL RAHLAADRHG GSVQVVSSTN
410 420 430 440
GELNTDDPTA GRSNAPITAP TEVEVMDETK CCCFFKRRKR KTIQRHK
Length:447
Mass (Da):51,389
Last modified:May 10, 2004 - v2
Checksum:i6FAEDD605579ED3A
GO
Isoform 2 (identifier: Q9Y6M4-2) [UniParc]FASTAAdd to basket
Also known as: 3L, CSNK1G3L

The sequence of this isoform differs from the canonical sequence as follows:
     430-430: K → NCQKVLNMW

Show »
Length:455
Mass (Da):52,378
Checksum:i23C1F32F3559120F
GO
Isoform 3 (identifier: Q9Y6M4-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     363-394: Missing.
     430-430: K → NCQKVLNMW

Show »
Length:423
Mass (Da):48,898
Checksum:i786AA3787897A4C5
GO
Isoform 4 (identifier: Q9Y6M4-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     282-282: E → EE
     363-394: Missing.
     430-430: K → NCQKVLNMW

Show »
Length:424
Mass (Da):49,027
Checksum:i0D2C64F25039E090
GO
Isoform 5 (identifier: Q9Y6M4-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-75: Missing.
     363-394: Missing.
     430-430: K → NCQKVLNMW

Show »
Length:348
Mass (Da):40,729
Checksum:i5AEB20AED88A1240
GO
Isoform 6 (identifier: Q9Y6M4-6) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-113: Missing.
     282-282: E → EE
     363-394: Missing.
     430-430: K → NCQKVLNMW

Show »
Length:311
Mass (Da):36,492
Checksum:i9A87163AF2FC9AFF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti91K → E in AAH47567 (PubMed:15489334).Curated1
Sequence conflicti174G → R in AAD26525 (PubMed:9925945).Curated1
Sequence conflicti174G → R in AAD26526 (PubMed:9925945).Curated1
Sequence conflicti241Y → C in BAH14390 (PubMed:14702039).Curated1
Sequence conflicti302D → E in AAD26525 (PubMed:9925945).Curated1
Sequence conflicti302D → E in AAD26526 (PubMed:9925945).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0470531 – 113Missing in isoform 6. 1 PublicationAdd BLAST113
Alternative sequenceiVSP_0470541 – 75Missing in isoform 5. 1 PublicationAdd BLAST75
Alternative sequenceiVSP_047055282E → EE in isoform 4 and isoform 6. 1 Publication1
Alternative sequenceiVSP_010256363 – 394Missing in isoform 3, isoform 4, isoform 5 and isoform 6. 2 PublicationsAdd BLAST32
Alternative sequenceiVSP_004749430K → NCQKVLNMW in isoform 2, isoform 3, isoform 4, isoform 5 and isoform 6. 3 Publications1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF049089 mRNA. Translation: AAD26525.1.
AF049090 mRNA. Translation: AAD26526.1.
AK289405 mRNA. Translation: BAF82094.1.
AK299739 mRNA. Translation: BAG61634.1.
AK316019 mRNA. Translation: BAH14390.1.
AC008541 Genomic DNA. No translation available.
AC026422 Genomic DNA. No translation available.
CH471086 Genomic DNA. Translation: EAW48872.1.
BC047567 mRNA. Translation: AAH47567.1.
CCDSiCCDS34218.1. [Q9Y6M4-3]
CCDS4135.1. [Q9Y6M4-1]
CCDS43355.1. [Q9Y6M4-2]
CCDS59491.1. [Q9Y6M4-4]
CCDS59492.1. [Q9Y6M4-5]
CCDS59493.1. [Q9Y6M4-6]
RefSeqiNP_001026982.1. NM_001031812.3. [Q9Y6M4-3]
NP_001038188.1. NM_001044723.2. [Q9Y6M4-2]
NP_001257501.1. NM_001270572.1. [Q9Y6M4-4]
NP_001257502.1. NM_001270573.1. [Q9Y6M4-5]
NP_001257503.1. NM_001270574.1. [Q9Y6M4-6]
NP_004375.2. NM_004384.4. [Q9Y6M4-1]
XP_005271949.1. XM_005271892.2. [Q9Y6M4-2]
XP_005271951.1. XM_005271894.2. [Q9Y6M4-1]
XP_005271952.1. XM_005271895.2. [Q9Y6M4-4]
XP_016864558.1. XM_017009069.1. [Q9Y6M4-4]
UniGeneiHs.129206.
Hs.741716.

Genome annotation databases

EnsembliENST00000345990; ENSP00000334735; ENSG00000151292. [Q9Y6M4-3]
ENST00000360683; ENSP00000353904; ENSG00000151292. [Q9Y6M4-2]
ENST00000361991; ENSP00000354942; ENSG00000151292. [Q9Y6M4-1]
ENST00000510842; ENSP00000423838; ENSG00000151292. [Q9Y6M4-4]
ENST00000511130; ENSP00000421385; ENSG00000151292. [Q9Y6M4-6]
ENST00000512718; ENSP00000421998; ENSG00000151292. [Q9Y6M4-5]
ENST00000521364; ENSP00000429412; ENSG00000151292. [Q9Y6M4-3]
GeneIDi1456.
KEGGihsa:1456.
UCSCiuc003ktn.5. human. [Q9Y6M4-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF049089 mRNA. Translation: AAD26525.1.
AF049090 mRNA. Translation: AAD26526.1.
AK289405 mRNA. Translation: BAF82094.1.
AK299739 mRNA. Translation: BAG61634.1.
AK316019 mRNA. Translation: BAH14390.1.
AC008541 Genomic DNA. No translation available.
AC026422 Genomic DNA. No translation available.
CH471086 Genomic DNA. Translation: EAW48872.1.
BC047567 mRNA. Translation: AAH47567.1.
CCDSiCCDS34218.1. [Q9Y6M4-3]
CCDS4135.1. [Q9Y6M4-1]
CCDS43355.1. [Q9Y6M4-2]
CCDS59491.1. [Q9Y6M4-4]
CCDS59492.1. [Q9Y6M4-5]
CCDS59493.1. [Q9Y6M4-6]
RefSeqiNP_001026982.1. NM_001031812.3. [Q9Y6M4-3]
NP_001038188.1. NM_001044723.2. [Q9Y6M4-2]
NP_001257501.1. NM_001270572.1. [Q9Y6M4-4]
NP_001257502.1. NM_001270573.1. [Q9Y6M4-5]
NP_001257503.1. NM_001270574.1. [Q9Y6M4-6]
NP_004375.2. NM_004384.4. [Q9Y6M4-1]
XP_005271949.1. XM_005271892.2. [Q9Y6M4-2]
XP_005271951.1. XM_005271894.2. [Q9Y6M4-1]
XP_005271952.1. XM_005271895.2. [Q9Y6M4-4]
XP_016864558.1. XM_017009069.1. [Q9Y6M4-4]
UniGeneiHs.129206.
Hs.741716.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2CHLX-ray1.95A35-362[»]
2IZRX-ray1.30A35-362[»]
2IZSX-ray1.95A35-362[»]
2IZTX-ray2.00A35-362[»]
2IZUX-ray1.85A35-362[»]
4G16X-ray2.30A34-362[»]
4G17X-ray2.10A34-362[»]
4HGLX-ray2.40A34-362[»]
4HGSX-ray2.40A34-362[»]
ProteinModelPortaliQ9Y6M4.
SMRiQ9Y6M4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi107840. 36 interactors.
IntActiQ9Y6M4. 6 interactors.
STRINGi9606.ENSP00000353904.

Chemistry databases

BindingDBiQ9Y6M4.
ChEMBLiCHEMBL5084.
GuidetoPHARMACOLOGYi2001.

PTM databases

iPTMnetiQ9Y6M4.
PhosphoSitePlusiQ9Y6M4.
SwissPalmiQ9Y6M4.

Polymorphism and mutation databases

DMDMi47117932.

Proteomic databases

EPDiQ9Y6M4.
PaxDbiQ9Y6M4.
PeptideAtlasiQ9Y6M4.
PRIDEiQ9Y6M4.

Protocols and materials databases

DNASUi1456.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000345990; ENSP00000334735; ENSG00000151292. [Q9Y6M4-3]
ENST00000360683; ENSP00000353904; ENSG00000151292. [Q9Y6M4-2]
ENST00000361991; ENSP00000354942; ENSG00000151292. [Q9Y6M4-1]
ENST00000510842; ENSP00000423838; ENSG00000151292. [Q9Y6M4-4]
ENST00000511130; ENSP00000421385; ENSG00000151292. [Q9Y6M4-6]
ENST00000512718; ENSP00000421998; ENSG00000151292. [Q9Y6M4-5]
ENST00000521364; ENSP00000429412; ENSG00000151292. [Q9Y6M4-3]
GeneIDi1456.
KEGGihsa:1456.
UCSCiuc003ktn.5. human. [Q9Y6M4-1]

Organism-specific databases

CTDi1456.
DisGeNETi1456.
GeneCardsiCSNK1G3.
HGNCiHGNC:2456. CSNK1G3.
HPAiHPA027010.
MIMi604253. gene.
neXtProtiNX_Q9Y6M4.
OpenTargetsiENSG00000151292.
PharmGKBiPA26956.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1165. Eukaryota.
ENOG410XNXP. LUCA.
GeneTreeiENSGT00760000119040.
HOGENOMiHOG000182054.
HOVERGENiHBG000176.
InParanoidiQ9Y6M4.
KOiK08958.
OMAiNREAHPH.
OrthoDBiEOG091G0F3Z.
PhylomeDBiQ9Y6M4.
TreeFamiTF313349.

Enzyme and pathway databases

BioCyciZFISH:HS07721-MONOMER.
BRENDAi2.7.11.1. 2681.
SignaLinkiQ9Y6M4.

Miscellaneous databases

ChiTaRSiCSNK1G3. human.
EvolutionaryTraceiQ9Y6M4.
GenomeRNAii1456.
PROiQ9Y6M4.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000151292.
CleanExiHS_CSNK1G3.
ExpressionAtlasiQ9Y6M4. baseline and differential.
GenevisibleiQ9Y6M4. HS.

Family and domain databases

InterProiIPR022247. Casein_kinase-1_gamma_C.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF12605. CK1gamma_C. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKC1G3_HUMAN
AccessioniPrimary (citable) accession number: Q9Y6M4
Secondary accession number(s): A8K040
, B4DSH2, B7Z9Q4, E7EVD0, Q86WZ7, Q9Y6M3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 10, 2004
Last modified: November 30, 2016
This is version 162 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

Triazolodiamine 1 is a commercial name for 5-amino-3-([4-(aminosulfonyl)phenyl]amino)-N-(2,6-difluorophenyl)-1H-1,2,4-triazole-1-carbothioamide.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.