Q9Y6M4 (KC1G3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Casein kinase I isoform gamma-3
Short name=CKI-gamma 3
EC=2.7.11.1

Gene names

Name:

CSNK1G3

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	447 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Serine/threonine-protein kinase. Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Regulates fast synaptic transmission mediated by glutamate By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Enzyme regulation	Inhibited by triazolodiamine 1, 5-amino-3-{[4-(aminosulfonyl)phenyl]amino}-N-(2,6-difluorophenyl)-1H-1,2,4-triazole-1-carbothioamide, (S)-propane-1,2-diol, 2-({6-[(3-chlorophenyl)amino]-9-isopropyl-9H-purin-2-yl}amino)-3-methylbutan-1-ol, N2-[(1R,2S)-2-aminocyclohexyl]-N6-(3-chlorophenyl)-9-ethyl-9H-purine-2,6-diamine and [4-amino-2-(3-chloroanilino)-1,3-thiazol-5-yl](4-fluorophenyl)methanone.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm.
Post-translational modification	Autophosphorylated By similarity.
Miscellaneous	Triazolodiamine 1 is a commercial name for 5-amino-3-([4-(aminosulfonyl)phenyl]amino)-N-(2,6-difluorophenyl)-1H-1,2,4-triazole-1-carbothioamide.
Sequence similarities	Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Wnt signaling pathway
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Wnt receptor signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW signal transduction Traceable author statement Ref.1. Source: ProtInc
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein kinase activity Traceable author statement Ref.1. Source: ProtInc protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9Y6M4-1)

Also known as: 3;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9Y6M4-2)

Also known as: 3L; CSNK1G3L;

The sequence of this isoform differs from the canonical sequence as follows:
     430-430: K → NCQKVLNMW

Isoform 3 (identifier: Q9Y6M4-3)

The sequence of this isoform differs from the canonical sequence as follows:
     363-394: Missing.
     430-430: K → NCQKVLNMW

Note: Contains a phosphoserine at position 365. Contains a phosphoserine at position 366.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 447

447

Casein kinase I isoform gamma-3

PRO_0000192846

Regions

Domain

43 – 313

271

Protein kinase

Nucleotide binding

49 – 57

9

ATP By similarity

Region

117 – 125

9

Inhibitors binding

Sites

Active site

162

1

Proton acceptor By similarity

Binding site

72

1

ATP By similarity

Amino acid modifications

Modified residue

413

1

Phosphoserine Ref.4

Natural variations

Alternative sequence

363 – 394

32

Missing in isoform 3.

VSP_010256

Alternative sequence

430

1

K → NCQKVLNMW in isoform 2 and isoform 3.

VSP_004749

Experimental info

Sequence conflict

91

1

K → E in AAH47567. Ref.2

Sequence conflict

174

1

G → R in AAD26525. Ref.1

Sequence conflict

174

1

G → R in AAD26526. Ref.1

Sequence conflict

302

1

D → E in AAD26525. Ref.1

Sequence conflict

302

1

D → E in AAD26526. Ref.1

Secondary structure

1

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447

Helix Strand Turn

Details...

Turn

40 – 42

3

Beta strand

43 – 48

6

Beta strand

50 – 52

3

Beta strand

56 – 62

7

Turn

63 – 66

4

Beta strand

67 – 75

9

Helix

83 – 93

11

Beta strand

102 – 108

7

Beta strand

111 – 117

7

Helix

123 – 129

7

Turn

130 – 132

3

Helix

136 – 155

20

Helix

165 – 167

3

Beta strand

168 – 170

3

Helix

173 – 175

3

Turn

176 – 179

4

Beta strand

180 – 183

4

Beta strand

190 – 193

4

Turn

195 – 197

3

Beta strand

198 – 200

3

Turn

213 – 215

3

Helix

218 – 221

4

Helix

228 – 244

17

Turn

248 – 251

4

Helix

257 – 270

14

Helix

273 – 276

4

Turn

277 – 279

3

Helix

281 – 292

12

Helix

301 – 314

14

Turn

324 – 327

4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (3) [UniParc]. Last modified May 10, 2004. Version 2. Checksum: 6FAEDD605579ED3A	FASTA	447	51,389
	10 20 30 40 50 60 MENKKKDKDK SDDRMARPSG RSGHNTRGTG SSSSGVLMVG PNFRVGKKIG CGNFGELRLG 70 80 90 100 110 120 KNLYTNEYVA IKLEPMKSRA PQLHLEYRFY KQLGSGDGIP QVYYFGPCGK YNAMVLELLG 130 140 150 160 170 180 PSLEDLFDLC DRTFSLKTVL MIAIQLISRM EYVHSKNLIY RDVKPENFLI GRPGNKTQQV 190 200 210 220 230 240 IHIIDFGLAK EYIDPETKKH IPYREHKSLT GTARYMSINT HLGKEQSRRD DLEALGHMFM 250 260 270 280 290 300 YFLRGSLPWQ GLKADTLKER YQKIGDTKRA TPIEVLCENF PEMATYLRYV RRLDFFEKPD 310 320 330 340 350 360 YDYLRKLFTD LFDRKGYMFD YEYDWIGKQL PTPVGAVQQD PALSSNREAH QHRDKMQQSK 370 380 390 400 410 420 NQSADHRAAW DSQQANPHHL RAHLAADRHG GSVQVVSSTN GELNTDDPTA GRSNAPITAP 430 440 TEVEVMDETK CCCFFKRRKR KTIQRHK
	Isoform 2 (3L) (CSNK1G3L) [UniParc]. Checksum: 23C1F32F3559120F	FASTA	455	52,378
	10 20 30 40 50 60 MENKKKDKDK SDDRMARPSG RSGHNTRGTG SSSSGVLMVG PNFRVGKKIG CGNFGELRLG 70 80 90 100 110 120 KNLYTNEYVA IKLEPMKSRA PQLHLEYRFY KQLGSGDGIP QVYYFGPCGK YNAMVLELLG 130 140 150 160 170 180 PSLEDLFDLC DRTFSLKTVL MIAIQLISRM EYVHSKNLIY RDVKPENFLI GRPGNKTQQV 190 200 210 220 230 240 IHIIDFGLAK EYIDPETKKH IPYREHKSLT GTARYMSINT HLGKEQSRRD DLEALGHMFM 250 260 270 280 290 300 YFLRGSLPWQ GLKADTLKER YQKIGDTKRA TPIEVLCENF PEMATYLRYV RRLDFFEKPD 310 320 330 340 350 360 YDYLRKLFTD LFDRKGYMFD YEYDWIGKQL PTPVGAVQQD PALSSNREAH QHRDKMQQSK 370 380 390 400 410 420 NQSADHRAAW DSQQANPHHL RAHLAADRHG GSVQVVSSTN GELNTDDPTA GRSNAPITAP 430 440 450 TEVEVMDETN CQKVLNMWCC CFFKRRKRKT IQRHK
	Isoform 3 [UniParc]. Checksum: 786AA3787897A4C5	FASTA	423	48,898
	10 20 30 40 50 60 MENKKKDKDK SDDRMARPSG RSGHNTRGTG SSSSGVLMVG PNFRVGKKIG CGNFGELRLG 70 80 90 100 110 120 KNLYTNEYVA IKLEPMKSRA PQLHLEYRFY KQLGSGDGIP QVYYFGPCGK YNAMVLELLG 130 140 150 160 170 180 PSLEDLFDLC DRTFSLKTVL MIAIQLISRM EYVHSKNLIY RDVKPENFLI GRPGNKTQQV 190 200 210 220 230 240 IHIIDFGLAK EYIDPETKKH IPYREHKSLT GTARYMSINT HLGKEQSRRD DLEALGHMFM 250 260 270 280 290 300 YFLRGSLPWQ GLKADTLKER YQKIGDTKRA TPIEVLCENF PEMATYLRYV RRLDFFEKPD 310 320 330 340 350 360 YDYLRKLFTD LFDRKGYMFD YEYDWIGKQL PTPVGAVQQD PALSSNREAH QHRDKMQQSK 370 380 390 400 410 420 NQVVSSTNGE LNTDDPTAGR SNAPITAPTE VEVMDETNCQ KVLNMWCCCF FKRRKRKTIQ RHK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and chromosome mapping of the human casein kinase I gamma3 gene (CSNK1G3)." Kusuda J., Hirai M., Toyoda A., Tanuma R., Hashimoto K. Cytogenet. Cell Genet. 83:101-103(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2). Tissue: Testis.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Brain.
[3]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[4]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-413, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[5]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[6]	"Large-scale proteomics analysis of the human kinome." Oppermann F.S., Gnad F., Olsen J.V., Hornberger R., Greff Z., Keri G., Mann M., Daub H. Mol. Cell. Proteomics 8:1751-1764(2009) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[7]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-366 (ISOFORM 3), MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[8]	"Structure of casein kinase 1 gamma 3." Bunkoczi G., Salah E., Rellos P., Das S., Fedorov O., Savitsky P., Gileadi O., Sundstrom M., Edwards A., Arrowsmith C., Ugochukwu E., Weigelt J., Von Delft F., Knapp S. Submitted (MAR-2006) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (1.3 ANGSTROMS) OF 35-362 IN COMPLEX WITH INHIBITORS.
[9]	"Inhibitor binding by casein kinases." Bunkoczi G., Salah E., Rellos P., Das S., Fedorov O., Savitsky P., Debreczeni J.E., Gileadi O., Sundstrom M., Edwards A., Arrowsmith C., Weigelt J., Von Delft F., Knapp S. Submitted (FEB-2009) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (1.30 ANGSTROMS) OF 35-362 IN COMPLEX WITH INHIBITORS.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF049089 mRNA. Translation: AAD26525.1.
AF049090 mRNA. Translation: AAD26526.1.
BC047567 mRNA. Translation: AAH47567.1.

IPI

IPI00181294.
IPI00218437.
IPI00941996.

RefSeq

NP_001026982.1. NM_001031812.3.
NP_001038188.1. NM_001044723.2.
NP_001257501.1. NM_001270572.1.
NP_004375.2. NM_004384.4.

UniGene

Hs.129206.
Hs.741716.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2CHL	X-ray	1.95	A	35-362	[»]
2IZR	X-ray	1.30	A	35-362	[»]
2IZS	X-ray	1.95	A	35-362	[»]
2IZT	X-ray	2.00	A	35-362	[»]
2IZU	X-ray	1.85	A	35-362	[»]
4HGL	X-ray	2.40	A	34-362	[»]
4HGS	X-ray	2.40	A	34-362	[»]

ProteinModelPortal

Q9Y6M4.

ModBase

Search...

Protein-protein interaction databases

STRING

9606.ENSP00000353904.

PTM databases

PhosphoSite

Q9Y6M4.

Polymorphism databases

DMDM

47117932.

Proteomic databases

PaxDb

Q9Y6M4.

PRIDE

Q9Y6M4.

Protocols and materials databases

DNASU

1456.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000345990; ENSP00000334735; ENSG00000151292.
ENST00000360683; ENSP00000353904; ENSG00000151292.
ENST00000361991; ENSP00000354942; ENSG00000151292.
ENST00000395411; ENSP00000378806; ENSG00000151292.
ENST00000395412; ENSP00000378807; ENSG00000151292.
ENST00000521364; ENSP00000429412; ENSG00000151292.

GeneID

1456.

KEGG

hsa:1456.

UCSC

uc003ktl.3. human.
uc003ktm.3. human.
uc003ktn.3. human.

Organism-specific databases

CTD

1456.

GeneCards

GC05P122909.

HGNC

HGNC:2456. CSNK1G3.

HPA

HPA027010.

MIM

604253. gene.

neXtProt

NX_Q9Y6M4.

PharmGKB

PA26956.

GenAtlas

Search...

Phylogenomic databases

eggNOG

COG0515.

HOGENOM

HOG000182054.

HOVERGEN

HBG000176.

KO

K08958.

OMA

NREAHPH.

OrthoDB

EOG4GHZP4.

Enzyme and pathway databases

BRENDA

2.7.11.1. 2681.

Pathway_Interaction_DB

foxopathway. FoxO family signaling.
hedgehog_glipathway. Hedgehog signaling events mediated by Gli proteins.

Gene expression databases

ArrayExpress

Q9Y6M4.

Bgee

Q9Y6M4.

CleanEx

HS_CSNK1G3.

Genevestigator

Q9Y6M4.

GermOnline

ENSG00000151292. Homo sapiens.

Family and domain databases

InterPro

IPR022247. Casein_kinase-1_gamma_C.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_cat_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]

Pfam

PF12605. CK1gamma_C. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]

SUPFAM

SSF56112. Kinase_like. 1 hit.

PROSITE

PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

BindingDB

Q9Y6M4.

ChEMBL

CHEMBL5084.

ChiTaRS

CSNK1G3. human.

EvolutionaryTrace

Q9Y6M4.

GenomeRNAi

1456.

NextBio

5979.

SOURCE

Search...

Entry information

Entry name

KC1G3_HUMAN

Accession

Primary (citable) accession number: Q9Y6M4
Secondary accession number(s): Q86WZ7, Q9Y6M3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 10, 2004
Last modified:	May 1, 2013
This is version 122 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.