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Protein

Insulin-like growth factor 2 mRNA-binding protein 2

Gene

IGF2BP2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

RNA-binding factor that recruits target transcripts to cytoplasmic protein-RNA complexes (mRNPs). This transcript 'caging' into mRNPs allows mRNA transport and transient storage. It also modulates the rate and location at which target transcripts encounter the translational apparatus and shields them from endonuclease attacks or microRNA-mediated degradation (By similarity). Binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNAs. Binding is isoform-specific. Binds to beta-actin/ACTB and MYC transcripts.By similarity2 Publications

GO - Molecular functioni

  • mRNA 3'-UTR binding Source: UniProtKB
  • mRNA 5'-UTR binding Source: BHF-UCL
  • nucleotide binding Source: InterPro
  • poly(A) RNA binding Source: UniProtKB
  • translation regulator activity Source: BHF-UCL

GO - Biological processi

  • anatomical structure morphogenesis Source: ProtInc
  • gene expression Source: Reactome
  • mRNA transport Source: UniProtKB-KW
  • negative regulation of translation Source: BHF-UCL
  • regulation of cytokine biosynthetic process Source: BHF-UCL
Complete GO annotation...

Keywords - Biological processi

mRNA transport, Translation regulation, Transport

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

BioCyciZFISH:ENSG00000073792-MONOMER.
ReactomeiR-HSA-428359. Insulin-like Growth Factor-2 mRNA Binding Proteins (IGF2BPs/IMPs/VICKZs) bind RNA.

Names & Taxonomyi

Protein namesi
Recommended name:
Insulin-like growth factor 2 mRNA-binding protein 2
Short name:
IGF2 mRNA-binding protein 2
Short name:
IMP-2
Alternative name(s):
Hepatocellular carcinoma autoantigen p62
IGF-II mRNA-binding protein 2
VICKZ family member 2
Gene namesi
Name:IGF2BP2
Synonyms:IMP2, VICKZ2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

HGNCiHGNC:28867. IGF2BP2.

Subcellular locationi

  • Nucleus
  • Cytoplasm

  • Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Localizes at the connecting piece and the tail of the spermatozoa. In response to cellular stress, such as oxidative stress, recruited to stress granules.

GO - Cellular componenti

  • cytoplasm Source: BHF-UCL
  • cytoskeleton Source: BHF-UCL
  • cytosol Source: Reactome
  • nucleus Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi211K → E: Significantly impaired binding to ACTB transcript, but little effect on MYC transcript binding, accumulation in the nucleus; when associated with E-292. Loss of homo- and heterooligomerization with IGF2BP1 and IGF2BP2, almost complete loss of ACTB and MYC transcript binding, almost complete loss of ELAVL1-, DHX9- and HNRNPU-binding and perturbed subcellular location, including accumulation in the nucleus and loss of localization to stress granules; when associated with E-292; 445-E-E-446 and 526-E-E-527. 1 Publication1
Mutagenesisi292K → E: Significantly impaired binding to ACTB transcript, but little effect on MYC transcript binding, accumulation in the nucleus; when associated with E-211. Loss of homo- and heterooligomerization with IGF2BP1 and IGF2BP2, almost complete loss of ACTB and MYC transcript binding, almost complete loss of ELAVL1-, DHX9- and HNRNPU-binding and perturbed subcellular location, including accumulation in the nucleus and loss of localization to stress granules; when associated with E-211; 445-E-E-445 and 526-E-E-527. 1 Publication1
Mutagenesisi445 – 446KK → EE: Significantly impaired binding to ACTB and MYC transcripts; when associated with 527-E-E-528. Loss of homo- and heterooligomerization with IGF2BP1 and IGF2BP2, almost complete loss of ACTB and MYC transcript binding, almost complete loss of ELAVL1-, DHX9- and HNRNPU-binding and perturbed subcellular location, including accumulation in the nucleus and loss of localization to stress granules; when associated with E-211; E-292 and 527-E-E-528. 1 Publication2
Mutagenesisi527 – 528KG → EE: Significantly impaired binding to ACTB and MYC transcripts; when associated with 445-E-E-446. Loss of homo- and heterooligomerization with IGF2BP1 and IGF2BP2, almost complete loss of ACTB and MYC transcript binding, almost complete loss of ELAVL1-, DHX9- and HNRNPU-binding and perturbed subcellular location, including accumulation in the nucleus and loss of localization to stress granules; when associated with E-211; E-292 and 445-E-E-446. 1 Publication2

Organism-specific databases

DisGeNETi10644.
MalaCardsiIGF2BP2.
OpenTargetsiENSG00000073792.
PharmGKBiPA128394577.

Polymorphism and mutation databases

BioMutaiIGF2BP2.
DMDMi224471831.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002444961 – 599Insulin-like growth factor 2 mRNA-binding protein 2Add BLAST599

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei11PhosphoserineCombined sources1
Modified residuei162PhosphoserineCombined sources1
Modified residuei164PhosphoserineCombined sources1
Modified residuei550PhosphothreonineBy similarity1
Isoform 6 (identifier: Q9Y6M1-6)
Modified residuei1N-acetylmethionineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9Y6M1.
MaxQBiQ9Y6M1.
PaxDbiQ9Y6M1.
PeptideAtlasiQ9Y6M1.
PRIDEiQ9Y6M1.

PTM databases

iPTMnetiQ9Y6M1.
PhosphoSitePlusiQ9Y6M1.
SwissPalmiQ9Y6M1.

Expressioni

Tissue specificityi

Expressed in oocytes, granulosa cells of small and growing follicles, Leydig cells, spermatogonia and semen (at protein level). Expressed in testicular cancer (at protein level). Expressed weakly in heart, placenta, skeletal muscle, bone marrow, colon, kidney, salivary glands, testis and pancreas. Detected in fetal liver, fetal ovary, gonocytes and interstitial cells of the testis.2 Publications

Gene expression databases

BgeeiENSG00000073792.
CleanExiHS_IGF2BP2.
ExpressionAtlasiQ9Y6M1. baseline and differential.
GenevisibleiQ9Y6M1. HS.

Organism-specific databases

HPAiCAB017126.
HPA035145.

Interactioni

Subunit structurei

Can form homooligomers and heterooligomers with IGF2BP1 and IGF2BP3 in an RNA-dependent manner. Interacts with HNRPD and IGF2BP1. Interacts with ELAVL1, DHX9 and HNRNPU.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
HNRNPDQ14103-44EBI-1024419,EBI-432545

Protein-protein interaction databases

BioGridi115888. 34 interactors.
IntActiQ9Y6M1. 30 interactors.
MINTiMINT-6946387.
STRINGi9606.ENSP00000371634.

Structurei

Secondary structure

1599
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi5 – 8Combined sources4
Helixi16 – 25Combined sources10
Beta strandi34 – 37Combined sources4
Beta strandi40 – 43Combined sources4
Helixi48 – 58Combined sources11
Turni59 – 61Combined sources3
Beta strandi70 – 73Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2CQHNMR-A2-81[»]
ProteinModelPortaliQ9Y6M1.
SMRiQ9Y6M1.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9Y6M1.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 76RRM 1PROSITE-ProRule annotationAdd BLAST74
Domaini82 – 157RRM 2PROSITE-ProRule annotationAdd BLAST76
Domaini193 – 258KH 1PROSITE-ProRule annotationAdd BLAST66
Domaini274 – 341KH 2PROSITE-ProRule annotationAdd BLAST68
Domaini427 – 492KH 3PROSITE-ProRule annotationAdd BLAST66
Domaini509 – 575KH 4PROSITE-ProRule annotationAdd BLAST67

Domaini

Domain KH3 and KH4 are the major RNA-binding modules, although KH1 and KH2 may also contribute. The contribution to RNA-binding of individual KH domains may be target-specific. KH1 and KH2, and possibly KH3 and KH4, promote the formation of higher ordered protein-RNA complexes, which may be essential for IGF2BP1 cytoplasmic retention. KH domains are required for RNA-dependent homo- and heterooligomerization and for localization to stress granules.2 Publications

Sequence similaritiesi

Belongs to the RRM IMP/VICKZ family.Curated
Contains 4 KH domains.PROSITE-ProRule annotation
Contains 2 RRM (RNA recognition motif) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG2193. Eukaryota.
ENOG410ZKB4. LUCA.
GeneTreeiENSGT00530000063171.
HOGENOMiHOG000000675.
HOVERGENiHBG052725.
InParanoidiQ9Y6M1.
KOiK17392.
PhylomeDBiQ9Y6M1.
TreeFamiTF320229.

Family and domain databases

Gene3Di3.30.1370.10. 4 hits.
3.30.70.330. 2 hits.
InterProiIPR028743. IGF2BP2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PANTHERiPTHR10288:SF93. PTHR10288:SF93. 1 hit.
PfamiPF00013. KH_1. 4 hits.
PF00076. RRM_1. 2 hits.
[Graphical view]
SMARTiSM00322. KH. 4 hits.
SM00360. RRM. 2 hits.
[Graphical view]
SUPFAMiSSF54791. SSF54791. 4 hits.
SSF54928. SSF54928. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 4 hits.
PS50102. RRM. 2 hits.
[Graphical view]

Sequences (6)i

Sequence statusi: Complete.

This entry describes 6 isoformsi produced by alternative splicing and alternative initiation. AlignAdd to basket

Isoform 1 (identifier: Q9Y6M1-2) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MMNKLYIGNL SPAVTADDLR QLFGDRKLPL AGQVLLKSGY AFVDYPDQNW
60 70 80 90 100
AIRAIETLSG KVELHGKIME VDYSVSKKLR SRKIQIRNIP PHLQWEVLDG
110 120 130 140 150
LLAQYGTVEN VEQVNTDTET AVVNVTYATR EEAKIAMEKL SGHQFENYSF
160 170 180 190 200
KISYIPDEEV SSPSPPQRAQ RGDHSSREQG HAPGGTSQAR QIDFPLRILV
210 220 230 240 250
PTQFVGAIIG KEGLTIKNIT KQTQSRVDIH RKENSGAAEK PVTIHATPEG
260 270 280 290 300
TSEACRMILE IMQKEADETK LAEEIPLKIL AHNGLVGRLI GKEGRNLKKI
310 320 330 340 350
EHETGTKITI SSLQDLSIYN PERTITVKGT VEACASAEIE IMKKLREAFE
360 370 380 390 400
NDMLAVNQQA NLIPGLNLSA LGIFSTGLSV LSPPAGPRGA PPAAPYHPFT
410 420 430 440 450
THSGYFSSLY PHHQFGPFPH HHSYPEQEIV NLFIPTQAVG AIIGKKGAHI
460 470 480 490 500
KQLARFAGAS IKIAPAEGPD VSERMVIITG PPEAQFKAQG RIFGKLKEEN
510 520 530 540 550
FFNPKEEVKL EAHIRVPSST AGRVIGKGGK TVNELQNLTS AEVIVPRDQT
560 570 580 590
PDENEEVIVR IIGHFFASQT AQRKIREIVQ QVKQQEQKYP QGVASQRSK
Length:599
Mass (Da):66,121
Last modified:March 3, 2009 - v2
Checksum:iEA656F8733BBB39A
GO
Isoform 2 (identifier: Q9Y6M1-1) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     358-400: Missing.

Show »
Length:556
Mass (Da):61,842
Checksum:i1EDEFB100443DDC4
GO
Isoform 3 (identifier: Q9Y6M1-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-80: MMNKLYIGNL...VDYSVSKKLR → MFSCPGHYHVDGFLNPG

Show »
Length:536
Mass (Da):59,001
Checksum:i733536C7BDAD8AF5
GO
Isoform 4 (identifier: Q9Y6M1-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-80: MMNKLYIGNL...VDYSVSKKLR → MFSCPGHYHVDGFLNPG
     113-113: Q → QVFAFSL

Show »
Length:542
Mass (Da):59,666
Checksum:iF1D98409DD8AE45D
GO
Isoform 5 (identifier: Q9Y6M1-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-80: MMNKLYIGNL...VDYSVSKKLR → MFSCPGHYHVDGFLNPG
     358-400: Missing.

Show »
Length:493
Mass (Da):54,722
Checksum:i36BF971E2C4296F7
GO
Isoform 6 (identifier: Q9Y6M1-6) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-68: Missing.

Note: Generated by alternative initiation at Met-69.Combined sources
Show »
Length:531
Mass (Da):58,578
Checksum:i1F5FD1AC97743530
GO

Sequence cautioni

The sequence AAH21290 differs from that shown. Reason: Erroneous initiation.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0365501 – 80MMNKL…SKKLR → MFSCPGHYHVDGFLNPG in isoform 3, isoform 4 and isoform 5. 1 PublicationAdd BLAST80
Alternative sequenceiVSP_0436991 – 68Missing in isoform 6. CuratedAdd BLAST68
Alternative sequenceiVSP_036552113Q → QVFAFSL in isoform 4. 1 Publication1
Alternative sequenceiVSP_036553358 – 400Missing in isoform 2 and isoform 5. 2 PublicationsAdd BLAST43

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF057352 mRNA. Translation: AAD31596.1.
EU408701 mRNA. Translation: ACB86625.1.
EU408702 mRNA. Translation: ACB86626.1.
EU408703 mRNA. Translation: ACB86627.1.
AC016961 Genomic DNA. No translation available.
AC108670 Genomic DNA. No translation available.
BC021290 mRNA. Translation: AAH21290.1. Different initiation.
CCDSiCCDS3273.2. [Q9Y6M1-2]
CCDS33903.1. [Q9Y6M1-1]
CCDS77869.1. [Q9Y6M1-3]
RefSeqiNP_001007226.1. NM_001007225.2. [Q9Y6M1-1]
NP_001278801.1. NM_001291872.2. [Q9Y6M1-4]
NP_001278802.1. NM_001291873.2. [Q9Y6M1-3]
NP_001278803.1. NM_001291874.2. [Q9Y6M1-5]
NP_001278804.1. NM_001291875.2.
NP_006539.3. NM_006548.5. [Q9Y6M1-2]
UniGeneiHs.293396.
Hs.35354.

Genome annotation databases

EnsembliENST00000346192; ENSP00000320204; ENSG00000073792. [Q9Y6M1-1]
ENST00000382199; ENSP00000371634; ENSG00000073792. [Q9Y6M1-2]
ENST00000421047; ENSP00000413787; ENSG00000073792. [Q9Y6M1-3]
GeneIDi10644.
KEGGihsa:10644.
UCSCiuc003fpo.4. human. [Q9Y6M1-2]

Keywords - Coding sequence diversityi

Alternative initiation, Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF057352 mRNA. Translation: AAD31596.1.
EU408701 mRNA. Translation: ACB86625.1.
EU408702 mRNA. Translation: ACB86626.1.
EU408703 mRNA. Translation: ACB86627.1.
AC016961 Genomic DNA. No translation available.
AC108670 Genomic DNA. No translation available.
BC021290 mRNA. Translation: AAH21290.1. Different initiation.
CCDSiCCDS3273.2. [Q9Y6M1-2]
CCDS33903.1. [Q9Y6M1-1]
CCDS77869.1. [Q9Y6M1-3]
RefSeqiNP_001007226.1. NM_001007225.2. [Q9Y6M1-1]
NP_001278801.1. NM_001291872.2. [Q9Y6M1-4]
NP_001278802.1. NM_001291873.2. [Q9Y6M1-3]
NP_001278803.1. NM_001291874.2. [Q9Y6M1-5]
NP_001278804.1. NM_001291875.2.
NP_006539.3. NM_006548.5. [Q9Y6M1-2]
UniGeneiHs.293396.
Hs.35354.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2CQHNMR-A2-81[»]
ProteinModelPortaliQ9Y6M1.
SMRiQ9Y6M1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115888. 34 interactors.
IntActiQ9Y6M1. 30 interactors.
MINTiMINT-6946387.
STRINGi9606.ENSP00000371634.

PTM databases

iPTMnetiQ9Y6M1.
PhosphoSitePlusiQ9Y6M1.
SwissPalmiQ9Y6M1.

Polymorphism and mutation databases

BioMutaiIGF2BP2.
DMDMi224471831.

Proteomic databases

EPDiQ9Y6M1.
MaxQBiQ9Y6M1.
PaxDbiQ9Y6M1.
PeptideAtlasiQ9Y6M1.
PRIDEiQ9Y6M1.

Protocols and materials databases

DNASUi10644.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000346192; ENSP00000320204; ENSG00000073792. [Q9Y6M1-1]
ENST00000382199; ENSP00000371634; ENSG00000073792. [Q9Y6M1-2]
ENST00000421047; ENSP00000413787; ENSG00000073792. [Q9Y6M1-3]
GeneIDi10644.
KEGGihsa:10644.
UCSCiuc003fpo.4. human. [Q9Y6M1-2]

Organism-specific databases

CTDi10644.
DisGeNETi10644.
GeneCardsiIGF2BP2.
HGNCiHGNC:28867. IGF2BP2.
HPAiCAB017126.
HPA035145.
MalaCardsiIGF2BP2.
MIMi608289. gene.
neXtProtiNX_Q9Y6M1.
OpenTargetsiENSG00000073792.
PharmGKBiPA128394577.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2193. Eukaryota.
ENOG410ZKB4. LUCA.
GeneTreeiENSGT00530000063171.
HOGENOMiHOG000000675.
HOVERGENiHBG052725.
InParanoidiQ9Y6M1.
KOiK17392.
PhylomeDBiQ9Y6M1.
TreeFamiTF320229.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000073792-MONOMER.
ReactomeiR-HSA-428359. Insulin-like Growth Factor-2 mRNA Binding Proteins (IGF2BPs/IMPs/VICKZs) bind RNA.

Miscellaneous databases

ChiTaRSiIGF2BP2. human.
EvolutionaryTraceiQ9Y6M1.
GeneWikiiIGF2BP2.
GenomeRNAii10644.
PROiQ9Y6M1.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000073792.
CleanExiHS_IGF2BP2.
ExpressionAtlasiQ9Y6M1. baseline and differential.
GenevisibleiQ9Y6M1. HS.

Family and domain databases

Gene3Di3.30.1370.10. 4 hits.
3.30.70.330. 2 hits.
InterProiIPR028743. IGF2BP2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PANTHERiPTHR10288:SF93. PTHR10288:SF93. 1 hit.
PfamiPF00013. KH_1. 4 hits.
PF00076. RRM_1. 2 hits.
[Graphical view]
SMARTiSM00322. KH. 4 hits.
SM00360. RRM. 2 hits.
[Graphical view]
SUPFAMiSSF54791. SSF54791. 4 hits.
SSF54928. SSF54928. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 4 hits.
PS50102. RRM. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIF2B2_HUMAN
AccessioniPrimary (citable) accession number: Q9Y6M1
Secondary accession number(s): A0A4Z0
, B3FTN2, B3FTN3, B3FTN4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: March 3, 2009
Last modified: November 30, 2016
This is version 146 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

Autoantibodies against IGF2BP2 are detected in sera from some patients with hepatocellular carcinoma.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.