SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q9Y6M0

- TEST_HUMAN

UniProt

Q9Y6M0 - TEST_HUMAN

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Testisin
Gene
PRSS21, ESP1, TEST1, UNQ266/PRO303
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Could regulate proteolytic events associated with testicular germ cell maturation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei82 – 821Charge relay system Reviewed prediction
Active sitei137 – 1371Charge relay system Reviewed prediction
Active sitei238 – 2381Charge relay system Reviewed prediction

GO - Molecular functioni

  1. protein binding Source: IntAct
  2. serine-type endopeptidase activity Source: Ensembl
  3. serine-type peptidase activity Source: ProtInc

GO - Biological processi

  1. spermatogenesis Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Protein family/group databases

MEROPSiS01.011.

Names & Taxonomyi

Protein namesi
Recommended name:
Testisin (EC:3.4.21.-)
Alternative name(s):
Eosinophil serine protease 1
Short name:
ESP-1
Serine protease 21
Gene namesi
Name:PRSS21
Synonyms:ESP1, TEST1
ORF Names:UNQ266/PRO303
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 16

Organism-specific databases

HGNCiHGNC:9485. PRSS21.

Subcellular locationi

Cell membrane; Lipid-anchorGPI-anchor Reviewed prediction

GO - Cellular componenti

  1. anchored component of membrane Source: UniProtKB-KW
  2. cytoplasm Source: ProtInc
  3. membrane Source: ProtInc
  4. plasma membrane Source: ProtInc
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA33834.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919 Reviewed prediction
Add
BLAST
Propeptidei20 – 4122 Reviewed prediction
PRO_0000027849Add
BLAST
Chaini42 – 288247Testisin
PRO_0000027850Add
BLAST
Propeptidei289 – 31426Removed in mature form Reviewed prediction
PRO_0000027851Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi33 ↔ 157 Reviewed prediction
Disulfide bondi67 ↔ 83 Reviewed prediction
Glycosylationi167 – 1671N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi171 ↔ 244 Reviewed prediction
Glycosylationi200 – 2001N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi204 ↔ 223 Reviewed prediction
Disulfide bondi234 ↔ 262 Reviewed prediction
Glycosylationi273 – 2731N-linked (GlcNAc...) Reviewed prediction
Lipidationi288 – 2881GPI-anchor amidated serine Reviewed prediction

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein, Zymogen

Proteomic databases

MaxQBiQ9Y6M0.
PaxDbiQ9Y6M0.
PRIDEiQ9Y6M0.

PTM databases

PhosphoSiteiQ9Y6M0.

Expressioni

Tissue specificityi

Expressed predominantly in premeiotic testicular germ cells, mostly late pachytene and diplotene spermatocytes.

Gene expression databases

ArrayExpressiQ9Y6M0.
BgeeiQ9Y6M0.
CleanExiHS_PRSS21.
GenevestigatoriQ9Y6M0.

Organism-specific databases

HPAiHPA008477.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
SERPINB5P369527EBI-7054564,EBI-2371394

Protein-protein interaction databases

BioGridi116142. 2 interactions.
IntActiQ9Y6M0. 2 interactions.
MINTiMINT-7712043.
STRINGi9606.ENSP00000005995.

Structurei

3D structure databases

ProteinModelPortaliQ9Y6M0.
SMRiQ9Y6M0. Positions 21-286.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini42 – 286245Peptidase S1
Add
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family.

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG5640.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiQ9Y6M0.
KOiK09625.
OMAiNSMCNHL.
OrthoDBiEOG75B84T.
PhylomeDBiQ9Y6M0.
TreeFamiTF351676.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9Y6M0-1) [UniParc]FASTAAdd to Basket

Also known as: L

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MGARGALLLA LLLARAGLRK PESQEAAPLS GPCGRRVITS RIVGGEDAEL    50
GRWPWQGSLR LWDSHVCGVS LLSHRWALTA AHCFETYSDL SDPSGWMVQF 100
GQLTSMPSFW SLQAYYTRYF VSNIYLSPRY LGNSPYDIAL VKLSAPVTYT 150
KHIQPICLQA STFEFENRTD CWVTGWGYIK EDEALPSPHT LQEVQVAIIN 200
NSMCNHLFLK YSFRKDIFGD MVCAGNAQGG KDACFGDSGG PLACNKNGLW 250
YQIGVVSWGV GCGRPNRPGV YTNISHHFEW IQKLMAQSGM SQPDPSWPLL 300
FFPLLWALPL LGPV 314
Length:314
Mass (Da):34,884
Last modified:November 1, 1999 - v1
Checksum:iE738CF73F6B56E98
GO
Isoform 2 (identifier: Q9Y6M0-2) [UniParc]FASTAAdd to Basket

Also known as: S

The sequence of this isoform differs from the canonical sequence as follows:
     87-88: Missing.

Show »
Length:312
Mass (Da):34,634
Checksum:i78BAFC2788E6AAF9
GO
Isoform 3 (identifier: Q9Y6M0-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     222-235: Missing.

Show »
Length:300
Mass (Da):33,562
Checksum:i427F0ABCD1B5E258
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti264 – 2641R → Q.
Corresponds to variant rs2072273 [ dbSNP | Ensembl ].
VAR_051840

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei87 – 882Missing in isoform 2.
VSP_005389
Alternative sequencei222 – 23514Missing in isoform 3.
VSP_005390Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF058300 mRNA. Translation: AAD41588.1.
AB031329 mRNA. Translation: BAA83520.1.
AB031330 mRNA. Translation: BAA83521.1.
AB031331 mRNA. Translation: BAA89532.1.
AF058301 Genomic DNA. Translation: AAF79019.1.
AF058301 Genomic DNA. Translation: AAF79020.1.
AY359034 mRNA. Translation: AAQ89393.1.
BC074999 mRNA. Translation: AAH74999.1.
BC075000 mRNA. Translation: AAH75000.1.
CCDSiCCDS10478.1. [Q9Y6M0-1]
CCDS45388.1. [Q9Y6M0-3]
CCDS45389.1. [Q9Y6M0-2]
RefSeqiNP_001257381.1. NM_001270452.1.
NP_006790.1. NM_006799.3. [Q9Y6M0-1]
NP_659205.1. NM_144956.2. [Q9Y6M0-2]
NP_659206.1. NM_144957.2. [Q9Y6M0-3]
UniGeneiHs.72026.

Genome annotation databases

EnsembliENST00000005995; ENSP00000005995; ENSG00000007038. [Q9Y6M0-1]
ENST00000450020; ENSP00000407741; ENSG00000007038. [Q9Y6M0-3]
ENST00000455114; ENSP00000400632; ENSG00000007038. [Q9Y6M0-2]
GeneIDi10942.
KEGGihsa:10942.
UCSCiuc002crr.4. human. [Q9Y6M0-3]
uc002crs.4. human. [Q9Y6M0-2]
uc002crt.4. human. [Q9Y6M0-1]

Polymorphism databases

DMDMi13633973.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF058300 mRNA. Translation: AAD41588.1 .
AB031329 mRNA. Translation: BAA83520.1 .
AB031330 mRNA. Translation: BAA83521.1 .
AB031331 mRNA. Translation: BAA89532.1 .
AF058301 Genomic DNA. Translation: AAF79019.1 .
AF058301 Genomic DNA. Translation: AAF79020.1 .
AY359034 mRNA. Translation: AAQ89393.1 .
BC074999 mRNA. Translation: AAH74999.1 .
BC075000 mRNA. Translation: AAH75000.1 .
CCDSi CCDS10478.1. [Q9Y6M0-1 ]
CCDS45388.1. [Q9Y6M0-3 ]
CCDS45389.1. [Q9Y6M0-2 ]
RefSeqi NP_001257381.1. NM_001270452.1.
NP_006790.1. NM_006799.3. [Q9Y6M0-1 ]
NP_659205.1. NM_144956.2. [Q9Y6M0-2 ]
NP_659206.1. NM_144957.2. [Q9Y6M0-3 ]
UniGenei Hs.72026.

3D structure databases

ProteinModelPortali Q9Y6M0.
SMRi Q9Y6M0. Positions 21-286.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 116142. 2 interactions.
IntActi Q9Y6M0. 2 interactions.
MINTi MINT-7712043.
STRINGi 9606.ENSP00000005995.

Protein family/group databases

MEROPSi S01.011.

PTM databases

PhosphoSitei Q9Y6M0.

Polymorphism databases

DMDMi 13633973.

Proteomic databases

MaxQBi Q9Y6M0.
PaxDbi Q9Y6M0.
PRIDEi Q9Y6M0.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000005995 ; ENSP00000005995 ; ENSG00000007038 . [Q9Y6M0-1 ]
ENST00000450020 ; ENSP00000407741 ; ENSG00000007038 . [Q9Y6M0-3 ]
ENST00000455114 ; ENSP00000400632 ; ENSG00000007038 . [Q9Y6M0-2 ]
GeneIDi 10942.
KEGGi hsa:10942.
UCSCi uc002crr.4. human. [Q9Y6M0-3 ]
uc002crs.4. human. [Q9Y6M0-2 ]
uc002crt.4. human. [Q9Y6M0-1 ]

Organism-specific databases

CTDi 10942.
GeneCardsi GC16P002867.
HGNCi HGNC:9485. PRSS21.
HPAi HPA008477.
MIMi 608159. gene.
neXtProti NX_Q9Y6M0.
PharmGKBi PA33834.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG5640.
HOGENOMi HOG000251820.
HOVERGENi HBG013304.
InParanoidi Q9Y6M0.
KOi K09625.
OMAi NSMCNHL.
OrthoDBi EOG75B84T.
PhylomeDBi Q9Y6M0.
TreeFami TF351676.

Miscellaneous databases

GenomeRNAii 10942.
NextBioi 41563.
PROi Q9Y6M0.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q9Y6M0.
Bgeei Q9Y6M0.
CleanExi HS_PRSS21.
Genevestigatori Q9Y6M0.

Family and domain databases

InterProi IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view ]
Pfami PF00089. Trypsin. 1 hit.
[Graphical view ]
PRINTSi PR00722. CHYMOTRYPSIN.
SMARTi SM00020. Tryp_SPc. 1 hit.
[Graphical view ]
SUPFAMi SSF50494. SSF50494. 1 hit.
PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and tissue distribution of a novel serine protease esp-1 from human eosinophils."
    Inoue M., Kanbe N., Kurosawa M., Kido H.
    Biochem. Biophys. Res. Commun. 252:307-312(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE (ISOFORM 1).
    Tissue: Eosinophil.
  2. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
  3. "Testisin, a new human serine proteinase expressed by premeiotic testicular germ cells and lost in testicular germ cell tumors."
    Hooper J.D., Nicol D.L., Dickinson J.L., Eyre H.J., Scarman A.L., Normyle J.F., Stuttgen M.A., Douglas M.L., Loveland K.A., Sutherland G.R., Antalis T.M.
    Cancer Res. 59:3199-3205(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE (ISOFORMS 1 AND 2).
    Tissue: Cervix carcinoma.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).

Entry informationi

Entry nameiTEST_HUMAN
AccessioniPrimary (citable) accession number: Q9Y6M0
Secondary accession number(s): Q9NS34, Q9P2V6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: November 1, 1999
Last modified: July 9, 2014
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Peptidase families
    Classification of peptidase families and list of entries
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi