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Protein

Testisin

Gene

PRSS21

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Could regulate proteolytic events associated with testicular germ cell maturation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei82 – 821Charge relay systemSequence Analysis
Active sitei137 – 1371Charge relay systemSequence Analysis
Active sitei238 – 2381Charge relay systemSequence Analysis

GO - Molecular functioni

  • serine-type endopeptidase activity Source: Ensembl
  • serine-type peptidase activity Source: ProtInc

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Protein family/group databases

MEROPSiS01.011.

Names & Taxonomyi

Protein namesi
Recommended name:
Testisin (EC:3.4.21.-)
Alternative name(s):
Eosinophil serine protease 1
Short name:
ESP-1
Serine protease 21
Gene namesi
Name:PRSS21
Synonyms:ESP1, TEST1
ORF Names:UNQ266/PRO303
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 16

Organism-specific databases

HGNCiHGNC:9485. PRSS21.

Subcellular locationi

GO - Cellular componenti

  • anchored component of membrane Source: UniProtKB-KW
  • cytoplasm Source: ProtInc
  • membrane Source: ProtInc
  • plasma membrane Source: ProtInc
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA33834.

Polymorphism and mutation databases

BioMutaiPRSS21.
DMDMi13633973.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Sequence AnalysisAdd
BLAST
Propeptidei20 – 4122Sequence AnalysisPRO_0000027849Add
BLAST
Chaini42 – 288247TestisinPRO_0000027850Add
BLAST
Propeptidei289 – 31426Removed in mature formSequence AnalysisPRO_0000027851Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi33 ↔ 157PROSITE-ProRule annotation
Disulfide bondi67 ↔ 83PROSITE-ProRule annotation
Glycosylationi167 – 1671N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi171 ↔ 244PROSITE-ProRule annotation
Glycosylationi200 – 2001N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi204 ↔ 223PROSITE-ProRule annotation
Disulfide bondi234 ↔ 262PROSITE-ProRule annotation
Glycosylationi273 – 2731N-linked (GlcNAc...)Sequence Analysis
Lipidationi288 – 2881GPI-anchor amidated serineSequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein, Zymogen

Proteomic databases

MaxQBiQ9Y6M0.
PaxDbiQ9Y6M0.
PRIDEiQ9Y6M0.

PTM databases

PhosphoSiteiQ9Y6M0.

Expressioni

Tissue specificityi

Expressed predominantly in premeiotic testicular germ cells, mostly late pachytene and diplotene spermatocytes.

Gene expression databases

BgeeiQ9Y6M0.
CleanExiHS_PRSS21.
ExpressionAtlasiQ9Y6M0. baseline and differential.
GenevestigatoriQ9Y6M0.

Organism-specific databases

HPAiHPA008477.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
SERPINB5P369527EBI-7054564,EBI-2371394

Protein-protein interaction databases

BioGridi116142. 2 interactions.
IntActiQ9Y6M0. 2 interactions.
MINTiMINT-7712043.
STRINGi9606.ENSP00000005995.

Structurei

3D structure databases

ProteinModelPortaliQ9Y6M0.
SMRiQ9Y6M0. Positions 42-286.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini42 – 286245Peptidase S1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG5640.
GeneTreeiENSGT00760000118810.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiQ9Y6M0.
KOiK09625.
OMAiNSMCNHL.
OrthoDBiEOG75B84T.
PhylomeDBiQ9Y6M0.
TreeFamiTF351676.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9Y6M0-1) [UniParc]FASTAAdd to basket

Also known as: L

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGARGALLLA LLLARAGLRK PESQEAAPLS GPCGRRVITS RIVGGEDAEL
60 70 80 90 100
GRWPWQGSLR LWDSHVCGVS LLSHRWALTA AHCFETYSDL SDPSGWMVQF
110 120 130 140 150
GQLTSMPSFW SLQAYYTRYF VSNIYLSPRY LGNSPYDIAL VKLSAPVTYT
160 170 180 190 200
KHIQPICLQA STFEFENRTD CWVTGWGYIK EDEALPSPHT LQEVQVAIIN
210 220 230 240 250
NSMCNHLFLK YSFRKDIFGD MVCAGNAQGG KDACFGDSGG PLACNKNGLW
260 270 280 290 300
YQIGVVSWGV GCGRPNRPGV YTNISHHFEW IQKLMAQSGM SQPDPSWPLL
310
FFPLLWALPL LGPV
Length:314
Mass (Da):34,884
Last modified:November 1, 1999 - v1
Checksum:iE738CF73F6B56E98
GO
Isoform 2 (identifier: Q9Y6M0-2) [UniParc]FASTAAdd to basket

Also known as: S

The sequence of this isoform differs from the canonical sequence as follows:
     87-88: Missing.

Show »
Length:312
Mass (Da):34,634
Checksum:i78BAFC2788E6AAF9
GO
Isoform 3 (identifier: Q9Y6M0-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     222-235: Missing.

Show »
Length:300
Mass (Da):33,562
Checksum:i427F0ABCD1B5E258
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti264 – 2641R → Q.
Corresponds to variant rs2072273 [ dbSNP | Ensembl ].
VAR_051840

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei87 – 882Missing in isoform 2. CuratedVSP_005389
Alternative sequencei222 – 23514Missing in isoform 3. 1 PublicationVSP_005390Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF058300 mRNA. Translation: AAD41588.1.
AB031329 mRNA. Translation: BAA83520.1.
AB031330 mRNA. Translation: BAA83521.1.
AB031331 mRNA. Translation: BAA89532.1.
AF058301 Genomic DNA. Translation: AAF79019.1.
AF058301 Genomic DNA. Translation: AAF79020.1.
AY359034 mRNA. Translation: AAQ89393.1.
BC074999 mRNA. Translation: AAH74999.1.
BC075000 mRNA. Translation: AAH75000.1.
CCDSiCCDS10478.1. [Q9Y6M0-1]
CCDS45388.1. [Q9Y6M0-3]
CCDS45389.1. [Q9Y6M0-2]
RefSeqiNP_001257381.1. NM_001270452.1.
NP_006790.1. NM_006799.3. [Q9Y6M0-1]
NP_659205.1. NM_144956.2. [Q9Y6M0-2]
NP_659206.1. NM_144957.2. [Q9Y6M0-3]
UniGeneiHs.72026.

Genome annotation databases

EnsembliENST00000005995; ENSP00000005995; ENSG00000007038. [Q9Y6M0-1]
ENST00000450020; ENSP00000407741; ENSG00000007038. [Q9Y6M0-3]
GeneIDi10942.
KEGGihsa:10942.
UCSCiuc002crr.4. human. [Q9Y6M0-3]
uc002crs.4. human. [Q9Y6M0-2]
uc002crt.4. human. [Q9Y6M0-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF058300 mRNA. Translation: AAD41588.1.
AB031329 mRNA. Translation: BAA83520.1.
AB031330 mRNA. Translation: BAA83521.1.
AB031331 mRNA. Translation: BAA89532.1.
AF058301 Genomic DNA. Translation: AAF79019.1.
AF058301 Genomic DNA. Translation: AAF79020.1.
AY359034 mRNA. Translation: AAQ89393.1.
BC074999 mRNA. Translation: AAH74999.1.
BC075000 mRNA. Translation: AAH75000.1.
CCDSiCCDS10478.1. [Q9Y6M0-1]
CCDS45388.1. [Q9Y6M0-3]
CCDS45389.1. [Q9Y6M0-2]
RefSeqiNP_001257381.1. NM_001270452.1.
NP_006790.1. NM_006799.3. [Q9Y6M0-1]
NP_659205.1. NM_144956.2. [Q9Y6M0-2]
NP_659206.1. NM_144957.2. [Q9Y6M0-3]
UniGeneiHs.72026.

3D structure databases

ProteinModelPortaliQ9Y6M0.
SMRiQ9Y6M0. Positions 42-286.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116142. 2 interactions.
IntActiQ9Y6M0. 2 interactions.
MINTiMINT-7712043.
STRINGi9606.ENSP00000005995.

Protein family/group databases

MEROPSiS01.011.

PTM databases

PhosphoSiteiQ9Y6M0.

Polymorphism and mutation databases

BioMutaiPRSS21.
DMDMi13633973.

Proteomic databases

MaxQBiQ9Y6M0.
PaxDbiQ9Y6M0.
PRIDEiQ9Y6M0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000005995; ENSP00000005995; ENSG00000007038. [Q9Y6M0-1]
ENST00000450020; ENSP00000407741; ENSG00000007038. [Q9Y6M0-3]
GeneIDi10942.
KEGGihsa:10942.
UCSCiuc002crr.4. human. [Q9Y6M0-3]
uc002crs.4. human. [Q9Y6M0-2]
uc002crt.4. human. [Q9Y6M0-1]

Organism-specific databases

CTDi10942.
GeneCardsiGC16P002867.
HGNCiHGNC:9485. PRSS21.
HPAiHPA008477.
MIMi608159. gene.
neXtProtiNX_Q9Y6M0.
PharmGKBiPA33834.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG5640.
GeneTreeiENSGT00760000118810.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiQ9Y6M0.
KOiK09625.
OMAiNSMCNHL.
OrthoDBiEOG75B84T.
PhylomeDBiQ9Y6M0.
TreeFamiTF351676.

Miscellaneous databases

ChiTaRSiPRSS21. human.
GenomeRNAii10942.
NextBioi41563.
PROiQ9Y6M0.
SOURCEiSearch...

Gene expression databases

BgeeiQ9Y6M0.
CleanExiHS_PRSS21.
ExpressionAtlasiQ9Y6M0. baseline and differential.
GenevestigatoriQ9Y6M0.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and tissue distribution of a novel serine protease esp-1 from human eosinophils."
    Inoue M., Kanbe N., Kurosawa M., Kido H.
    Biochem. Biophys. Res. Commun. 252:307-312(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE (ISOFORM 1).
    Tissue: Eosinophil.
  2. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
  3. "Testisin, a new human serine proteinase expressed by premeiotic testicular germ cells and lost in testicular germ cell tumors."
    Hooper J.D., Nicol D.L., Dickinson J.L., Eyre H.J., Scarman A.L., Normyle J.F., Stuttgen M.A., Douglas M.L., Loveland K.A., Sutherland G.R., Antalis T.M.
    Cancer Res. 59:3199-3205(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE (ISOFORMS 1 AND 2).
    Tissue: Cervix carcinoma.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).

Entry informationi

Entry nameiTEST_HUMAN
AccessioniPrimary (citable) accession number: Q9Y6M0
Secondary accession number(s): Q9NS34, Q9P2V6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: November 1, 1999
Last modified: April 29, 2015
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Peptidase families
    Classification of peptidase families and list of entries
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.