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Q9Y6M0

- TEST_HUMAN

UniProt

Q9Y6M0 - TEST_HUMAN

Protein

Testisin

Gene

PRSS21

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 128 (01 Oct 2014)
      Sequence version 1 (01 Nov 1999)
      Previous versions | rss
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    Functioni

    Could regulate proteolytic events associated with testicular germ cell maturation.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei82 – 821Charge relay systemSequence Analysis
    Active sitei137 – 1371Charge relay systemSequence Analysis
    Active sitei238 – 2381Charge relay systemSequence Analysis

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. serine-type endopeptidase activity Source: Ensembl
    3. serine-type peptidase activity Source: ProtInc

    GO - Biological processi

    1. spermatogenesis Source: Ensembl

    Keywords - Molecular functioni

    Hydrolase, Protease, Serine protease

    Protein family/group databases

    MEROPSiS01.011.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Testisin (EC:3.4.21.-)
    Alternative name(s):
    Eosinophil serine protease 1
    Short name:
    ESP-1
    Serine protease 21
    Gene namesi
    Name:PRSS21
    Synonyms:ESP1, TEST1
    ORF Names:UNQ266/PRO303
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 16

    Organism-specific databases

    HGNCiHGNC:9485. PRSS21.

    Subcellular locationi

    GO - Cellular componenti

    1. anchored component of membrane Source: UniProtKB-KW
    2. cytoplasm Source: ProtInc
    3. membrane Source: ProtInc
    4. plasma membrane Source: ProtInc

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA33834.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1919Sequence AnalysisAdd
    BLAST
    Propeptidei20 – 4122Sequence AnalysisPRO_0000027849Add
    BLAST
    Chaini42 – 288247TestisinPRO_0000027850Add
    BLAST
    Propeptidei289 – 31426Removed in mature formSequence AnalysisPRO_0000027851Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi33 ↔ 157PROSITE-ProRule annotation
    Disulfide bondi67 ↔ 83PROSITE-ProRule annotation
    Glycosylationi167 – 1671N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi171 ↔ 244PROSITE-ProRule annotation
    Glycosylationi200 – 2001N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi204 ↔ 223PROSITE-ProRule annotation
    Disulfide bondi234 ↔ 262PROSITE-ProRule annotation
    Glycosylationi273 – 2731N-linked (GlcNAc...)Sequence Analysis
    Lipidationi288 – 2881GPI-anchor amidated serineSequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein, Zymogen

    Proteomic databases

    MaxQBiQ9Y6M0.
    PaxDbiQ9Y6M0.
    PRIDEiQ9Y6M0.

    PTM databases

    PhosphoSiteiQ9Y6M0.

    Expressioni

    Tissue specificityi

    Expressed predominantly in premeiotic testicular germ cells, mostly late pachytene and diplotene spermatocytes.

    Gene expression databases

    ArrayExpressiQ9Y6M0.
    BgeeiQ9Y6M0.
    CleanExiHS_PRSS21.
    GenevestigatoriQ9Y6M0.

    Organism-specific databases

    HPAiHPA008477.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    SERPINB5P369527EBI-7054564,EBI-2371394

    Protein-protein interaction databases

    BioGridi116142. 2 interactions.
    IntActiQ9Y6M0. 2 interactions.
    MINTiMINT-7712043.
    STRINGi9606.ENSP00000005995.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9Y6M0.
    SMRiQ9Y6M0. Positions 21-286.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini42 – 286245Peptidase S1PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase S1 family.PROSITE-ProRule annotation
    Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG5640.
    HOGENOMiHOG000251820.
    HOVERGENiHBG013304.
    InParanoidiQ9Y6M0.
    KOiK09625.
    OMAiNSMCNHL.
    OrthoDBiEOG75B84T.
    PhylomeDBiQ9Y6M0.
    TreeFamiTF351676.

    Family and domain databases

    InterProiIPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view]
    PfamiPF00089. Trypsin. 1 hit.
    [Graphical view]
    PRINTSiPR00722. CHYMOTRYPSIN.
    SMARTiSM00020. Tryp_SPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF50494. SSF50494. 1 hit.
    PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9Y6M0-1) [UniParc]FASTAAdd to Basket

    Also known as: L

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MGARGALLLA LLLARAGLRK PESQEAAPLS GPCGRRVITS RIVGGEDAEL    50
    GRWPWQGSLR LWDSHVCGVS LLSHRWALTA AHCFETYSDL SDPSGWMVQF 100
    GQLTSMPSFW SLQAYYTRYF VSNIYLSPRY LGNSPYDIAL VKLSAPVTYT 150
    KHIQPICLQA STFEFENRTD CWVTGWGYIK EDEALPSPHT LQEVQVAIIN 200
    NSMCNHLFLK YSFRKDIFGD MVCAGNAQGG KDACFGDSGG PLACNKNGLW 250
    YQIGVVSWGV GCGRPNRPGV YTNISHHFEW IQKLMAQSGM SQPDPSWPLL 300
    FFPLLWALPL LGPV 314
    Length:314
    Mass (Da):34,884
    Last modified:November 1, 1999 - v1
    Checksum:iE738CF73F6B56E98
    GO
    Isoform 2 (identifier: Q9Y6M0-2) [UniParc]FASTAAdd to Basket

    Also known as: S

    The sequence of this isoform differs from the canonical sequence as follows:
         87-88: Missing.

    Show »
    Length:312
    Mass (Da):34,634
    Checksum:i78BAFC2788E6AAF9
    GO
    Isoform 3 (identifier: Q9Y6M0-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         222-235: Missing.

    Show »
    Length:300
    Mass (Da):33,562
    Checksum:i427F0ABCD1B5E258
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti264 – 2641R → Q.
    Corresponds to variant rs2072273 [ dbSNP | Ensembl ].
    VAR_051840

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei87 – 882Missing in isoform 2. CuratedVSP_005389
    Alternative sequencei222 – 23514Missing in isoform 3. 1 PublicationVSP_005390Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF058300 mRNA. Translation: AAD41588.1.
    AB031329 mRNA. Translation: BAA83520.1.
    AB031330 mRNA. Translation: BAA83521.1.
    AB031331 mRNA. Translation: BAA89532.1.
    AF058301 Genomic DNA. Translation: AAF79019.1.
    AF058301 Genomic DNA. Translation: AAF79020.1.
    AY359034 mRNA. Translation: AAQ89393.1.
    BC074999 mRNA. Translation: AAH74999.1.
    BC075000 mRNA. Translation: AAH75000.1.
    CCDSiCCDS10478.1. [Q9Y6M0-1]
    CCDS45388.1. [Q9Y6M0-3]
    CCDS45389.1. [Q9Y6M0-2]
    RefSeqiNP_001257381.1. NM_001270452.1.
    NP_006790.1. NM_006799.3. [Q9Y6M0-1]
    NP_659205.1. NM_144956.2. [Q9Y6M0-2]
    NP_659206.1. NM_144957.2. [Q9Y6M0-3]
    UniGeneiHs.72026.

    Genome annotation databases

    EnsembliENST00000005995; ENSP00000005995; ENSG00000007038. [Q9Y6M0-1]
    ENST00000450020; ENSP00000407741; ENSG00000007038. [Q9Y6M0-3]
    GeneIDi10942.
    KEGGihsa:10942.
    UCSCiuc002crr.4. human. [Q9Y6M0-3]
    uc002crs.4. human. [Q9Y6M0-2]
    uc002crt.4. human. [Q9Y6M0-1]

    Polymorphism databases

    DMDMi13633973.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF058300 mRNA. Translation: AAD41588.1 .
    AB031329 mRNA. Translation: BAA83520.1 .
    AB031330 mRNA. Translation: BAA83521.1 .
    AB031331 mRNA. Translation: BAA89532.1 .
    AF058301 Genomic DNA. Translation: AAF79019.1 .
    AF058301 Genomic DNA. Translation: AAF79020.1 .
    AY359034 mRNA. Translation: AAQ89393.1 .
    BC074999 mRNA. Translation: AAH74999.1 .
    BC075000 mRNA. Translation: AAH75000.1 .
    CCDSi CCDS10478.1. [Q9Y6M0-1 ]
    CCDS45388.1. [Q9Y6M0-3 ]
    CCDS45389.1. [Q9Y6M0-2 ]
    RefSeqi NP_001257381.1. NM_001270452.1.
    NP_006790.1. NM_006799.3. [Q9Y6M0-1 ]
    NP_659205.1. NM_144956.2. [Q9Y6M0-2 ]
    NP_659206.1. NM_144957.2. [Q9Y6M0-3 ]
    UniGenei Hs.72026.

    3D structure databases

    ProteinModelPortali Q9Y6M0.
    SMRi Q9Y6M0. Positions 21-286.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 116142. 2 interactions.
    IntActi Q9Y6M0. 2 interactions.
    MINTi MINT-7712043.
    STRINGi 9606.ENSP00000005995.

    Protein family/group databases

    MEROPSi S01.011.

    PTM databases

    PhosphoSitei Q9Y6M0.

    Polymorphism databases

    DMDMi 13633973.

    Proteomic databases

    MaxQBi Q9Y6M0.
    PaxDbi Q9Y6M0.
    PRIDEi Q9Y6M0.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000005995 ; ENSP00000005995 ; ENSG00000007038 . [Q9Y6M0-1 ]
    ENST00000450020 ; ENSP00000407741 ; ENSG00000007038 . [Q9Y6M0-3 ]
    GeneIDi 10942.
    KEGGi hsa:10942.
    UCSCi uc002crr.4. human. [Q9Y6M0-3 ]
    uc002crs.4. human. [Q9Y6M0-2 ]
    uc002crt.4. human. [Q9Y6M0-1 ]

    Organism-specific databases

    CTDi 10942.
    GeneCardsi GC16P002867.
    HGNCi HGNC:9485. PRSS21.
    HPAi HPA008477.
    MIMi 608159. gene.
    neXtProti NX_Q9Y6M0.
    PharmGKBi PA33834.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG5640.
    HOGENOMi HOG000251820.
    HOVERGENi HBG013304.
    InParanoidi Q9Y6M0.
    KOi K09625.
    OMAi NSMCNHL.
    OrthoDBi EOG75B84T.
    PhylomeDBi Q9Y6M0.
    TreeFami TF351676.

    Miscellaneous databases

    GenomeRNAii 10942.
    NextBioi 41563.
    PROi Q9Y6M0.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9Y6M0.
    Bgeei Q9Y6M0.
    CleanExi HS_PRSS21.
    Genevestigatori Q9Y6M0.

    Family and domain databases

    InterProi IPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view ]
    Pfami PF00089. Trypsin. 1 hit.
    [Graphical view ]
    PRINTSi PR00722. CHYMOTRYPSIN.
    SMARTi SM00020. Tryp_SPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50494. SSF50494. 1 hit.
    PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and tissue distribution of a novel serine protease esp-1 from human eosinophils."
      Inoue M., Kanbe N., Kurosawa M., Kido H.
      Biochem. Biophys. Res. Commun. 252:307-312(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE (ISOFORM 1).
      Tissue: Eosinophil.
    2. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
    3. "Testisin, a new human serine proteinase expressed by premeiotic testicular germ cells and lost in testicular germ cell tumors."
      Hooper J.D., Nicol D.L., Dickinson J.L., Eyre H.J., Scarman A.L., Normyle J.F., Stuttgen M.A., Douglas M.L., Loveland K.A., Sutherland G.R., Antalis T.M.
      Cancer Res. 59:3199-3205(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE (ISOFORMS 1 AND 2).
      Tissue: Cervix carcinoma.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).

    Entry informationi

    Entry nameiTEST_HUMAN
    AccessioniPrimary (citable) accession number: Q9Y6M0
    Secondary accession number(s): Q9NS34, Q9P2V6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 27, 2001
    Last sequence update: November 1, 1999
    Last modified: October 1, 2014
    This is version 128 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 16
      Human chromosome 16: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. Peptidase families
      Classification of peptidase families and list of entries
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3