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Protein

Testisin

Gene

PRSS21

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Could regulate proteolytic events associated with testicular germ cell maturation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei82Charge relay systemSequence analysis1
Active sitei137Charge relay systemSequence analysis1
Active sitei238Charge relay systemSequence analysis1

GO - Molecular functioni

  • serine-type endopeptidase activity Source: Ensembl
  • serine-type peptidase activity Source: ProtInc

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Serine protease

Enzyme and pathway databases

ReactomeiR-HSA-163125 Post-translational modification: synthesis of GPI-anchored proteins
SIGNORiQ9Y6M0

Protein family/group databases

MEROPSiS01.011

Names & Taxonomyi

Protein namesi
Recommended name:
Testisin (EC:3.4.21.-)
Alternative name(s):
Eosinophil serine protease 1
Short name:
ESP-1
Serine protease 21
Gene namesi
Name:PRSS21
Synonyms:ESP1, TEST1
ORF Names:UNQ266/PRO303
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi

Organism-specific databases

EuPathDBiHostDB:ENSG00000007038.10
HGNCiHGNC:9485 PRSS21
MIMi608159 gene
neXtProtiNX_Q9Y6M0

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi10942
OpenTargetsiENSG00000007038
PharmGKBiPA33834

Polymorphism and mutation databases

BioMutaiPRSS21
DMDMi13633973

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
PropeptideiPRO_000002784920 – 41Sequence analysisAdd BLAST22
ChainiPRO_000002785042 – 288TestisinAdd BLAST247
PropeptideiPRO_0000027851289 – 314Removed in mature formSequence analysisAdd BLAST26

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi33 ↔ 157PROSITE-ProRule annotation
Disulfide bondi67 ↔ 83PROSITE-ProRule annotation
Glycosylationi167N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi171 ↔ 244PROSITE-ProRule annotation
Glycosylationi200N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi204 ↔ 223PROSITE-ProRule annotation
Disulfide bondi234 ↔ 262PROSITE-ProRule annotation
Glycosylationi273N-linked (GlcNAc...) asparagineSequence analysis1
Lipidationi288GPI-anchor amidated serineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein, Zymogen

Proteomic databases

EPDiQ9Y6M0
PaxDbiQ9Y6M0
PeptideAtlasiQ9Y6M0
PRIDEiQ9Y6M0

PTM databases

iPTMnetiQ9Y6M0
PhosphoSitePlusiQ9Y6M0

Expressioni

Tissue specificityi

Expressed predominantly in premeiotic testicular germ cells, mostly late pachytene and diplotene spermatocytes.

Gene expression databases

BgeeiENSG00000007038
CleanExiHS_PRSS21
ExpressionAtlasiQ9Y6M0 baseline and differential
GenevisibleiQ9Y6M0 HS

Organism-specific databases

HPAiHPA008477

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
SERPINB5P369527EBI-7054564,EBI-2371394

Protein-protein interaction databases

BioGridi116142, 26 interactors
IntActiQ9Y6M0, 14 interactors
MINTiQ9Y6M0
STRINGi9606.ENSP00000005995

Structurei

3D structure databases

ProteinModelPortaliQ9Y6M0
SMRiQ9Y6M0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini42 – 286Peptidase S1PROSITE-ProRule annotationAdd BLAST245

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3627 Eukaryota
COG5640 LUCA
GeneTreeiENSGT00900000140825
HOGENOMiHOG000251820
HOVERGENiHBG013304
InParanoidiQ9Y6M0
KOiK09625
OMAiIINNSMC
OrthoDBiEOG091G0AH5
PhylomeDBiQ9Y6M0
TreeFamiTF351676

Family and domain databases

CDDicd00190 Tryp_SPc, 1 hit
InterProiView protein in InterPro
IPR009003 Peptidase_S1_PA
IPR001314 Peptidase_S1A
IPR034380 Testisin
IPR001254 Trypsin_dom
IPR018114 TRYPSIN_HIS
IPR033116 TRYPSIN_SER
PANTHERiPTHR24256:SF173 PTHR24256:SF173, 1 hit
PfamiView protein in Pfam
PF00089 Trypsin, 1 hit
PRINTSiPR00722 CHYMOTRYPSIN
SMARTiView protein in SMART
SM00020 Tryp_SPc, 1 hit
SUPFAMiSSF50494 SSF50494, 1 hit
PROSITEiView protein in PROSITE
PS50240 TRYPSIN_DOM, 1 hit
PS00134 TRYPSIN_HIS, 1 hit
PS00135 TRYPSIN_SER, 1 hit

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9Y6M0-1) [UniParc]FASTAAdd to basket
Also known as: L

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGARGALLLA LLLARAGLRK PESQEAAPLS GPCGRRVITS RIVGGEDAEL
60 70 80 90 100
GRWPWQGSLR LWDSHVCGVS LLSHRWALTA AHCFETYSDL SDPSGWMVQF
110 120 130 140 150
GQLTSMPSFW SLQAYYTRYF VSNIYLSPRY LGNSPYDIAL VKLSAPVTYT
160 170 180 190 200
KHIQPICLQA STFEFENRTD CWVTGWGYIK EDEALPSPHT LQEVQVAIIN
210 220 230 240 250
NSMCNHLFLK YSFRKDIFGD MVCAGNAQGG KDACFGDSGG PLACNKNGLW
260 270 280 290 300
YQIGVVSWGV GCGRPNRPGV YTNISHHFEW IQKLMAQSGM SQPDPSWPLL
310
FFPLLWALPL LGPV
Length:314
Mass (Da):34,884
Last modified:November 1, 1999 - v1
Checksum:iE738CF73F6B56E98
GO
Isoform 2 (identifier: Q9Y6M0-2) [UniParc]FASTAAdd to basket
Also known as: S

The sequence of this isoform differs from the canonical sequence as follows:
     87-88: Missing.

Show »
Length:312
Mass (Da):34,634
Checksum:i78BAFC2788E6AAF9
GO
Isoform 3 (identifier: Q9Y6M0-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     222-235: Missing.

Show »
Length:300
Mass (Da):33,562
Checksum:i427F0ABCD1B5E258
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_051840264R → Q. Corresponds to variant dbSNP:rs2072273Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_00538987 – 88Missing in isoform 2. Curated2
Alternative sequenceiVSP_005390222 – 235Missing in isoform 3. 1 PublicationAdd BLAST14

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF058300 mRNA Translation: AAD41588.1
AB031329 mRNA Translation: BAA83520.1
AB031330 mRNA Translation: BAA83521.1
AB031331 mRNA Translation: BAA89532.1
AF058301 Genomic DNA Translation: AAF79019.1
AF058301 Genomic DNA Translation: AAF79020.1
AY359034 mRNA Translation: AAQ89393.1
BC074999 mRNA Translation: AAH74999.1
BC075000 mRNA Translation: AAH75000.1
CCDSiCCDS10478.1 [Q9Y6M0-1]
CCDS45388.1 [Q9Y6M0-3]
RefSeqiNP_001257381.1, NM_001270452.1
NP_006790.1, NM_006799.3 [Q9Y6M0-1]
NP_659205.1, NM_144956.2 [Q9Y6M0-2]
NP_659206.1, NM_144957.2 [Q9Y6M0-3]
UniGeneiHs.72026

Genome annotation databases

EnsembliENST00000005995; ENSP00000005995; ENSG00000007038 [Q9Y6M0-1]
ENST00000450020; ENSP00000407741; ENSG00000007038 [Q9Y6M0-3]
ENST00000634351; ENSP00000489315; ENSG00000282838 [Q9Y6M0-1]
ENST00000634454; ENSP00000489081; ENSG00000282838 [Q9Y6M0-3]
GeneIDi10942
KEGGihsa:10942
UCSCiuc002crr.5 human [Q9Y6M0-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Entry informationi

Entry nameiTEST_HUMAN
AccessioniPrimary (citable) accession number: Q9Y6M0
Secondary accession number(s): Q9NS34, Q9P2V6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: November 1, 1999
Last modified: May 23, 2018
This is version 158 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Peptidase families
    Classification of peptidase families and list of entries
  6. SIMILARITY comments
    Index of protein domains and families

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