Q9Y6M0 (TEST_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Testisin
EC=3.4.21.-

Alternative name(s):
Eosinophil serine protease 1
Short name=ESP-1
Serine protease 21

Gene names

Name:	PRSS21
Synonyms:	ESP1, TEST1
ORF Names:	UNQ266/PRO303

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	314 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Could regulate proteolytic events associated with testicular germ cell maturation.
Subcellular location	Cell membrane; Lipid-anchor › GPI-anchor Potential.
Tissue specificity	Expressed predominantly in premeiotic testicular germ cells, mostly late pachytene and diplotene spermatocytes.
Sequence similarities	Belongs to the peptidase S1 family. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond GPI-anchor Glycoprotein Lipoprotein Zymogen
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	anchored to membrane Inferred from electronic annotation. Source: UniProtKB-KW cytoplasm Inferred from direct assay. Source: HPA plasma membrane Traceable author statement Ref.3. Source: ProtInc
Molecular_function	serine-type endopeptidase activity Inferred from electronic annotation. Source: Compara serine-type peptidase activity Traceable author statement Ref.3. Source: ProtInc
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9Y6M0-1)

Also known as: L;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9Y6M0-2)

Also known as: S;

The sequence of this isoform differs from the canonical sequence as follows:
     87-88: Missing.

Isoform 3 (identifier: Q9Y6M0-3)

The sequence of this isoform differs from the canonical sequence as follows:
     222-235: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Propeptide	20 – 41	22	Potential		PRO_0000027849
	Chain	42 – 288	247	Testisin		PRO_0000027850
	Propeptide	289 – 314	26	Removed in mature form Potential		PRO_0000027851
Regions
	Domain	42 – 286	245	Peptidase S1
Sites
	Active site	82	1	Charge relay system Potential
	Active site	137	1	Charge relay system Potential
	Active site	238	1	Charge relay system Potential
Amino acid modifications
	Lipidation	288	1	GPI-anchor amidated serine Potential
	Glycosylation	167	1	N-linked (GlcNAc...) Potential
	Glycosylation	200	1	N-linked (GlcNAc...) Potential
	Glycosylation	273	1	N-linked (GlcNAc...) Potential
	Disulfide bond	33 ↔ 157		Potential
	Disulfide bond	67 ↔ 83		Potential
	Disulfide bond	171 ↔ 244		Potential
	Disulfide bond	204 ↔ 223		Potential
	Disulfide bond	234 ↔ 262		Potential
Natural variations
	Alternative sequence	87 – 88	2	Missing in isoform 2.		VSP_005389
	Alternative sequence	222 – 235	14	Missing in isoform 3.		VSP_005390
	Natural variant	264	1	R → Q. Corresponds to variant rs2072273 [ dbSNP | Ensembl ].		VAR_051840

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (L) [UniParc]. Last modified November 1, 1999. Version 1. Checksum: E738CF73F6B56E98	FASTA	314	34,884
	10 20 30 40 50 60 MGARGALLLA LLLARAGLRK PESQEAAPLS GPCGRRVITS RIVGGEDAEL GRWPWQGSLR 70 80 90 100 110 120 LWDSHVCGVS LLSHRWALTA AHCFETYSDL SDPSGWMVQF GQLTSMPSFW SLQAYYTRYF 130 140 150 160 170 180 VSNIYLSPRY LGNSPYDIAL VKLSAPVTYT KHIQPICLQA STFEFENRTD CWVTGWGYIK 190 200 210 220 230 240 EDEALPSPHT LQEVQVAIIN NSMCNHLFLK YSFRKDIFGD MVCAGNAQGG KDACFGDSGG 250 260 270 280 290 300 PLACNKNGLW YQIGVVSWGV GCGRPNRPGV YTNISHHFEW IQKLMAQSGM SQPDPSWPLL 310 FFPLLWALPL LGPV
	Isoform 2 (S) [UniParc]. Checksum: 78BAFC2788E6AAF9	FASTA	312	34,634
	10 20 30 40 50 60 MGARGALLLA LLLARAGLRK PESQEAAPLS GPCGRRVITS RIVGGEDAEL GRWPWQGSLR 70 80 90 100 110 120 LWDSHVCGVS LLSHRWALTA AHCFETDLSD PSGWMVQFGQ LTSMPSFWSL QAYYTRYFVS 130 140 150 160 170 180 NIYLSPRYLG NSPYDIALVK LSAPVTYTKH IQPICLQAST FEFENRTDCW VTGWGYIKED 190 200 210 220 230 240 EALPSPHTLQ EVQVAIINNS MCNHLFLKYS FRKDIFGDMV CAGNAQGGKD ACFGDSGGPL 250 260 270 280 290 300 ACNKNGLWYQ IGVVSWGVGC GRPNRPGVYT NISHHFEWIQ KLMAQSGMSQ PDPSWPLLFF 310 PLLWALPLLG PV
	Isoform 3 [UniParc]. Checksum: 427F0ABCD1B5E258	FASTA	300	33,562
	10 20 30 40 50 60 MGARGALLLA LLLARAGLRK PESQEAAPLS GPCGRRVITS RIVGGEDAEL GRWPWQGSLR 70 80 90 100 110 120 LWDSHVCGVS LLSHRWALTA AHCFETYSDL SDPSGWMVQF GQLTSMPSFW SLQAYYTRYF 130 140 150 160 170 180 VSNIYLSPRY LGNSPYDIAL VKLSAPVTYT KHIQPICLQA STFEFENRTD CWVTGWGYIK 190 200 210 220 230 240 EDEALPSPHT LQEVQVAIIN NSMCNHLFLK YSFRKDIFGD MGDSGGPLAC NKNGLWYQIG 250 260 270 280 290 300 VVSWGVGCGR PNRPGVYTNI SHHFEWIQKL MAQSGMSQPD PSWPLLFFPL LWALPLLGPV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and tissue distribution of a novel serine protease esp-1 from human eosinophils." Inoue M., Kanbe N., Kurosawa M., Kido H. Biochem. Biophys. Res. Commun. 252:307-312(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE (ISOFORM 1). Tissue: Eosinophil.
[2]	"Structural analysis of esp-1 gene (PRSS 21)." Inoue M., Isobe M., Itoyama T., Kido H. Biochem. Biophys. Res. Commun. 266:564-568(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
[3]	"Testisin, a new human serine proteinase expressed by premeiotic testicular germ cells and lost in testicular germ cell tumors." Hooper J.D., Nicol D.L., Dickinson J.L., Eyre H.J., Scarman A.L., Normyle J.F., Stuttgen M.A., Douglas M.L., Loveland K.A., Sutherland G.R., Antalis T.M. Cancer Res. 59:3199-3205(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE (ISOFORMS 1 AND 2). Tissue: Cervix carcinoma.
[4]	"Localization, expression and genomic structure of the gene encoding the human serine protease testisin." Hooper J.D., Bowen N., Marshall H., Cullen L.M., Sood R., Daniels R., Stuttgen M.A., Normyle J.F., Higgs D.R., Kastner D.L., Ogbourne S.M., Pera M.F., Jazwinska E.C., Antalis T.M. Biochim. Biophys. Acta 1492:63-71(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]	"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M. Genome Res. 13:2265-2270(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF058300 mRNA. Translation: AAD41588.1. AB031329 mRNA. Translation: BAA83520.1. AB031330 mRNA. Translation: BAA83521.1. AB031331 mRNA. Translation: BAA89532.1. AF058301 Genomic DNA. Translation: AAF79019.1. AF058301 Genomic DNA. Translation: AAF79020.1. AY359034 mRNA. Translation: AAQ89393.1. BC074999 mRNA. Translation: AAH74999.1. BC075000 mRNA. Translation: AAH75000.1.
IPI	IPI00160581. IPI00218172. IPI00305703.
RefSeq	NP_001257381.1. NM_001270452.1. NP_006790.1. NM_006799.3. NP_659205.1. NM_144956.2. NP_659206.1. NM_144957.2.
UniGene	Hs.72026.
3D structure databases
ProteinModelPortal	Q9Y6M0.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000005995.
Protein family/group databases
MEROPS	S01.011.
PTM databases
PhosphoSite	Q9Y6M0.
Polymorphism databases
DMDM	13633973.
Proteomic databases
PaxDb	Q9Y6M0.
PRIDE	Q9Y6M0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000005995; ENSP00000005995; ENSG00000007038. ENST00000450020; ENSP00000407741; ENSG00000007038. ENST00000455114; ENSP00000400632; ENSG00000007038.
GeneID	10942.
KEGG	hsa:10942.
UCSC	uc002crr.3. human. uc002crs.3. human. uc002crt.3. human.
Organism-specific databases
CTD	10942.
GeneCards	GC16P002867.
HGNC	HGNC:9485. PRSS21.
HPA	HPA008477.
MIM	608159. gene.
neXtProt	NX_Q9Y6M0.
PharmGKB	PA33834.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG5640.
HOGENOM	HOG000251820.
HOVERGEN	HBG013304.
InParanoid	Q9Y6M0.
KO	K09625.
OMA	LTSMPSF.
OrthoDB	EOG47WNPQ.
PhylomeDB	Q9Y6M0.
Gene expression databases
ArrayExpress	Q9Y6M0.
Bgee	Q9Y6M0.
CleanEx	HS_PRSS21.
Genevestigator	Q9Y6M0.
GermOnline	ENSG00000007038. Homo sapiens.
Family and domain databases
InterPro	IPR001254. Peptidase_S1. IPR018114. Peptidase_S1_AS. IPR001314. Peptidase_S1A. IPR009003. Trypsin-like_Pept_dom. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
SUPFAM	SSF50494. Pept_Ser_Cys. 1 hit.
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	10942.
NextBio	41563.
SOURCE	Search...

Entry information

Entry name

TEST_HUMAN

Accession

Primary (citable) accession number: Q9Y6M0
Secondary accession number(s): Q9NS34, Q9P2V6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	November 1, 1999
Last modified:	May 1, 2013
This is version 115 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.