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Q9Y6M0 (TEST_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 124. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Testisin

EC=3.4.21.-
Alternative name(s):
Eosinophil serine protease 1
Short name=ESP-1
Serine protease 21
Gene names
Name:PRSS21
Synonyms:ESP1, TEST1
ORF Names:UNQ266/PRO303
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length314 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Could regulate proteolytic events associated with testicular germ cell maturation.

Subcellular location

Cell membrane; Lipid-anchorGPI-anchor Potential.

Tissue specificity

Expressed predominantly in premeiotic testicular germ cells, mostly late pachytene and diplotene spermatocytes.

Sequence similarities

Belongs to the peptidase S1 family.

Contains 1 peptidase S1 domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

SERPINB5P369527EBI-7054564,EBI-2371394

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9Y6M0-1)

Also known as: L;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9Y6M0-2)

Also known as: S;

The sequence of this isoform differs from the canonical sequence as follows:
     87-88: Missing.
Isoform 3 (identifier: Q9Y6M0-3)

The sequence of this isoform differs from the canonical sequence as follows:
     222-235: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1919 Potential
Propeptide20 – 4122 Potential
PRO_0000027849
Chain42 – 288247Testisin
PRO_0000027850
Propeptide289 – 31426Removed in mature form Potential
PRO_0000027851

Regions

Domain42 – 286245Peptidase S1

Sites

Active site821Charge relay system Potential
Active site1371Charge relay system Potential
Active site2381Charge relay system Potential

Amino acid modifications

Lipidation2881GPI-anchor amidated serine Potential
Glycosylation1671N-linked (GlcNAc...) Potential
Glycosylation2001N-linked (GlcNAc...) Potential
Glycosylation2731N-linked (GlcNAc...) Potential
Disulfide bond33 ↔ 157 Potential
Disulfide bond67 ↔ 83 Potential
Disulfide bond171 ↔ 244 Potential
Disulfide bond204 ↔ 223 Potential
Disulfide bond234 ↔ 262 Potential

Natural variations

Alternative sequence87 – 882Missing in isoform 2.
VSP_005389
Alternative sequence222 – 23514Missing in isoform 3.
VSP_005390
Natural variant2641R → Q.
Corresponds to variant rs2072273 [ dbSNP | Ensembl ].
VAR_051840

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (L) [UniParc].

Last modified November 1, 1999. Version 1.
Checksum: E738CF73F6B56E98

FASTA31434,884
        10         20         30         40         50         60 
MGARGALLLA LLLARAGLRK PESQEAAPLS GPCGRRVITS RIVGGEDAEL GRWPWQGSLR 

        70         80         90        100        110        120 
LWDSHVCGVS LLSHRWALTA AHCFETYSDL SDPSGWMVQF GQLTSMPSFW SLQAYYTRYF 

       130        140        150        160        170        180 
VSNIYLSPRY LGNSPYDIAL VKLSAPVTYT KHIQPICLQA STFEFENRTD CWVTGWGYIK 

       190        200        210        220        230        240 
EDEALPSPHT LQEVQVAIIN NSMCNHLFLK YSFRKDIFGD MVCAGNAQGG KDACFGDSGG 

       250        260        270        280        290        300 
PLACNKNGLW YQIGVVSWGV GCGRPNRPGV YTNISHHFEW IQKLMAQSGM SQPDPSWPLL 

       310 
FFPLLWALPL LGPV 

« Hide

Isoform 2 (S) [UniParc].

Checksum: 78BAFC2788E6AAF9
Show »

FASTA31234,634
Isoform 3 [UniParc].

Checksum: 427F0ABCD1B5E258
Show »

FASTA30033,562

References

« Hide 'large scale' references
[1]"Cloning and tissue distribution of a novel serine protease esp-1 from human eosinophils."
Inoue M., Kanbe N., Kurosawa M., Kido H.
Biochem. Biophys. Res. Commun. 252:307-312(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE (ISOFORM 1).
Tissue: Eosinophil.
[2]"Structural analysis of esp-1 gene (PRSS 21)."
Inoue M., Isobe M., Itoyama T., Kido H.
Biochem. Biophys. Res. Commun. 266:564-568(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
[3]"Testisin, a new human serine proteinase expressed by premeiotic testicular germ cells and lost in testicular germ cell tumors."
Hooper J.D., Nicol D.L., Dickinson J.L., Eyre H.J., Scarman A.L., Normyle J.F., Stuttgen M.A., Douglas M.L., Loveland K.A., Sutherland G.R., Antalis T.M.
Cancer Res. 59:3199-3205(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE (ISOFORMS 1 AND 2).
Tissue: Cervix carcinoma.
[4]"Localization, expression and genomic structure of the gene encoding the human serine protease testisin."
Hooper J.D., Bowen N., Marshall H., Cullen L.M., Sood R., Daniels R., Stuttgen M.A., Normyle J.F., Higgs D.R., Kastner D.L., Ogbourne S.M., Pera M.F., Jazwinska E.C., Antalis T.M.
Biochim. Biophys. Acta 1492:63-71(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment."
Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. expand/collapse author list , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.
Genome Res. 13:2265-2270(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF058300 mRNA. Translation: AAD41588.1.
AB031329 mRNA. Translation: BAA83520.1.
AB031330 mRNA. Translation: BAA83521.1.
AB031331 mRNA. Translation: BAA89532.1.
AF058301 Genomic DNA. Translation: AAF79019.1.
AF058301 Genomic DNA. Translation: AAF79020.1.
AY359034 mRNA. Translation: AAQ89393.1.
BC074999 mRNA. Translation: AAH74999.1.
BC075000 mRNA. Translation: AAH75000.1.
RefSeqNP_001257381.1. NM_001270452.1.
NP_006790.1. NM_006799.3.
NP_659205.1. NM_144956.2.
NP_659206.1. NM_144957.2.
UniGeneHs.72026.

3D structure databases

ProteinModelPortalQ9Y6M0.
SMRQ9Y6M0. Positions 42-286.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid116142. 2 interactions.
IntActQ9Y6M0. 2 interactions.
MINTMINT-7712043.
STRING9606.ENSP00000005995.

Protein family/group databases

MEROPSS01.011.

PTM databases

PhosphoSiteQ9Y6M0.

Polymorphism databases

DMDM13633973.

Proteomic databases

PaxDbQ9Y6M0.
PRIDEQ9Y6M0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000005995; ENSP00000005995; ENSG00000007038. [Q9Y6M0-1]
ENST00000450020; ENSP00000407741; ENSG00000007038. [Q9Y6M0-3]
ENST00000455114; ENSP00000400632; ENSG00000007038. [Q9Y6M0-2]
GeneID10942.
KEGGhsa:10942.
UCSCuc002crr.4. human. [Q9Y6M0-3]
uc002crs.4. human. [Q9Y6M0-2]
uc002crt.4. human. [Q9Y6M0-1]

Organism-specific databases

CTD10942.
GeneCardsGC16P002867.
HGNCHGNC:9485. PRSS21.
HPAHPA008477.
MIM608159. gene.
neXtProtNX_Q9Y6M0.
PharmGKBPA33834.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG5640.
HOGENOMHOG000251820.
HOVERGENHBG013304.
InParanoidQ9Y6M0.
KOK09625.
OMALTSMPSF.
OrthoDBEOG75B84T.
PhylomeDBQ9Y6M0.
TreeFamTF351676.

Gene expression databases

ArrayExpressQ9Y6M0.
BgeeQ9Y6M0.
CleanExHS_PRSS21.
GenevestigatorQ9Y6M0.

Family and domain databases

InterProIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSPR00722. CHYMOTRYPSIN.
SMARTSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMSSF50494. SSF50494. 1 hit.
PROSITEPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi10942.
NextBio41563.
PROQ9Y6M0.
SOURCESearch...

Entry information

Entry nameTEST_HUMAN
AccessionPrimary (citable) accession number: Q9Y6M0
Secondary accession number(s): Q9NS34, Q9P2V6
Entry history
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: November 1, 1999
Last modified: April 16, 2014
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 16

Human chromosome 16: entries, gene names and cross-references to MIM