Q9Y6L6 (SO1B1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Solute carrier organic anion transporter family member 1B1

Alternative name(s):
Liver-specific organic anion transporter 1
Short name=LST-1
OATP-C
Sodium-independent organic anion-transporting polypeptide 2
Short name=OATP-2
Solute carrier family 21 member 6

Gene names

Name:	SLCO1B1
Synonyms:	LST1, OATP1B1, OATP2, OATPC, SLC21A6

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	691 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Mediates the Na+-independent uptake of organic anions such as pravastatin, taurocholate, methotrexate, dehydroepiandrosterone sulfate, 17-beta-glucuronosyl estradiol, estrone sulfate, prostaglandin E2, thromboxane B2, leukotriene C3, leukotriene E4, thyroxine and triiodothyronine. Involved in the clearance of bile acids and organic anions from the liver. Ref.1 Ref.2 Ref.10 Ref.12
Subcellular location	Basolateral cell membrane; Multi-pass membrane protein. Note: Detected in basolateral membranes of hepatocytes. Ref.12
Tissue specificity	Highly expressed in liver, at the basolateral membranes of centrilobular hepatocytes. Not detected in heart, brain, placenta, lung, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon and leukocyte. Ref.1 Ref.2 Ref.10
Involvement in disease	Hyperbilirubinemia, Rotor type (HBLRR) [MIM:237450]: An autosomal recessive form of primary conjugated hyperbilirubinemia. Affected individuals develop mild jaundice not associated with hemolysis shortly after birth or in childhood. They have delayed plasma clearance of the unconjugated anionic dye bromsulphthalein and prominent urinary excretion of coproporphyrin I. Hepatic pigmentation is normal. Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.10
Sequence similarities	Belongs to the organo anion transporter (TC 2.A.60) family. [View classification] Contains 1 Kazal-like domain.

Ontologies

Keywords
Biological process	Ion transport Transport
Cellular component	Cell membrane Membrane
Coding sequence diversity	Polymorphism
Domain	Transmembrane Transmembrane helix
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	bile acid and bile salt transport Traceable author statement. Source: Reactome bile acid metabolic process Traceable author statement. Source: Reactome sodium-independent organic anion transport Traceable author statement. Source: Reactome
Cellular_component	basolateral plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to plasma membrane Traceable author statement Ref.1. Source: ProtInc
Molecular_function	sodium-independent organic anion transmembrane transporter activity Traceable author statement Ref.1. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 691	691	Solute carrier organic anion transporter family member 1B1		PRO_0000191050
Regions
	Topological domain	1 – 28	28	Cytoplasmic Potential
	Transmembrane	29 – 48	20	Helical; Name=1; Potential
	Topological domain	49 – 67	19	Extracellular Potential
	Transmembrane	68 – 88	21	Helical; Name=2; Potential
	Topological domain	89 – 94	6	Cytoplasmic Potential
	Transmembrane	95 – 119	25	Helical; Name=3; Potential
	Topological domain	120 – 168	49	Extracellular Potential
	Transmembrane	169 – 197	29	Helical; Name=4; Potential
	Topological domain	198 – 216	19	Cytoplasmic Potential
	Transmembrane	217 – 237	21	Helical; Name=5; Potential
	Topological domain	238 – 255	18	Extracellular Potential
	Transmembrane	256 – 280	25	Helical; Name=6; Potential
	Topological domain	281 – 331	51	Cytoplasmic Potential
	Transmembrane	332 – 353	22	Helical; Name=7; Potential
	Topological domain	354 – 373	20	Extracellular Potential
	Transmembrane	374 – 397	24	Helical; Name=8; Potential
	Topological domain	398 – 401	4	Cytoplasmic Potential
	Transmembrane	402 – 425	24	Helical; Name=9; Potential
	Topological domain	426 – 537	112	Extracellular Potential
	Transmembrane	538 – 560	23	Helical; Name=10; Potential
	Topological domain	561 – 569	9	Cytoplasmic Potential
	Transmembrane	570 – 595	26	Helical; Name=11; Potential
	Topological domain	596 – 629	34	Extracellular Potential
	Transmembrane	630 – 647	18	Helical; Name=12; Potential
	Topological domain	648 – 691	44	Cytoplasmic Potential
	Domain	453 – 508	56	Kazal-like
Amino acid modifications
	Glycosylation	130	1	N-linked (GlcNAc...) Potential
	Glycosylation	134	1	N-linked (GlcNAc...) Potential
	Glycosylation	432	1	N-linked (GlcNAc...) Potential
	Glycosylation	503	1	N-linked (GlcNAc...) Potential
	Glycosylation	516	1	N-linked (GlcNAc...) Potential
	Glycosylation	617	1	N-linked (GlcNAc...) Potential
	Disulfide bond	459 ↔ 485		By similarity
	Disulfide bond	463 ↔ 474		By similarity
	Disulfide bond	465 ↔ 489		By similarity
Natural variations
	Natural variant	73	1	F → L. Ref.11 Corresponds to variant rs56101265 [ dbSNP | Ensembl ].		VAR_015070
	Natural variant	82	1	V → A. Ref.11 Corresponds to variant rs56061388 [ dbSNP | Ensembl ].		VAR_015071
	Natural variant	130	1	N → D. Ref.2 Ref.6 Ref.9 Ref.11 Ref.12 Ref.13 Corresponds to variant rs2306283 [ dbSNP | Ensembl ].		VAR_015072
	Natural variant	151	1	N → S. Corresponds to variant rs2306282 [ dbSNP | Ensembl ].		VAR_057724
	Natural variant	152	1	R → K. Ref.1 Ref.11 Ref.13		VAR_015073
	Natural variant	155	1	P → T Decreased transport activity. Ref.9 Ref.11 Ref.12 Corresponds to variant rs11045819 [ dbSNP | Ensembl ].		VAR_015074
	Natural variant	156	1	E → G Decreased transport activity. Ref.11		VAR_015075
	Natural variant	174	1	V → A Decreased transport activity. Ref.11 Ref.13 Corresponds to variant rs4149056 [ dbSNP | Ensembl ].		VAR_015076
	Natural variant	193	1	L → R Strongly decreases expression at the plasma membrane; abolishes transport activity. Ref.12		VAR_015077
	Natural variant	241	1	D → N. Ref.1 Ref.11 Ref.13		VAR_015078
	Natural variant	245	1	I → V. Corresponds to variant rs11045852 [ dbSNP | Ensembl ].		VAR_060108
	Natural variant	353	1	I → T. Ref.11 Corresponds to variant rs55901008 [ dbSNP | Ensembl ].		VAR_015079
	Natural variant	432	1	N → D. Ref.11 Corresponds to variant rs56387224 [ dbSNP | Ensembl ].		VAR_015080
	Natural variant	462	1	D → G. Ref.11		VAR_015081
	Natural variant	488	1	G → A. Ref.11 Corresponds to variant rs59502379 [ dbSNP | Ensembl ].		VAR_015082
	Natural variant	643	1	L → F. Corresponds to variant rs34671512 [ dbSNP | Ensembl ].		VAR_057725
	Natural variant	655	1	D → G. Ref.11 Corresponds to variant rs56199088 [ dbSNP | Ensembl ].		VAR_015083
	Natural variant	667	1	E → G. Ref.11 Corresponds to variant rs55737008 [ dbSNP | Ensembl ].		VAR_015084

Sequences

	Sequence		Length	Mass (Da)
	Q9Y6L6 [UniParc]. Last modified December 1, 2000. Version 2. Checksum: D13D5130189383A3	FASTA	691	76,449
	10 20 30 40 50 60 MDQNQHLNKT AEAQPSENKK TRYCNGLKMF LAALSLSFIA KTLGAIIMKS SIIHIERRFE 70 80 90 100 110 120 ISSSLVGFID GSFEIGNLLV IVFVSYFGSK LHRPKLIGIG CFIMGIGGVL TALPHFFMGY 130 140 150 160 170 180 YRYSKETNIN SSENSTSTLS TCLINQILSL NRASPEIVGK GCLKESGSYM WIYVFMGNML 190 200 210 220 230 240 RGIGETPIVP LGLSYIDDFA KEGHSSLYLG ILNAIAMIGP IIGFTLGSLF SKMYVDIGYV 250 260 270 280 290 300 DLSTIRITPT DSRWVGAWWL NFLVSGLFSI ISSIPFFFLP QTPNKPQKER KASLSLHVLE 310 320 330 340 350 360 TNDEKDQTAN LTNQGKNITK NVTGFFQSFK SILTNPLYVM FVLLTLLQVS SYIGAFTYVF 370 380 390 400 410 420 KYVEQQYGQP SSKANILLGV ITIPIFASGM FLGGYIIKKF KLNTVGIAKF SCFTAVMSLS 430 440 450 460 470 480 FYLLYFFILC ENKSVAGLTM TYDGNNPVTS HRDVPLSYCN SDCNCDESQW EPVCGNNGIT 490 500 510 520 530 540 YISPCLAGCK SSSGNKKPIV FYNCSCLEVT GLQNRNYSAH LGECPRDDAC TRKFYFFVAI 550 560 570 580 590 600 QVLNLFFSAL GGTSHVMLIV KIVQPELKSL ALGFHSMVIR ALGGILAPIY FGALIDTTCI 610 620 630 640 650 660 KWSTNNCGTR GSCRTYNSTS FSRVYLGLSS MLRVSSLVLY IILIYAMKKK YQEKDINASE 670 680 690 NGSVMDEANL ESLNKNKHFV PSAGADSETH C

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of a novel gene family encoding human liver-specific organic anion transporter LST-1." Abe T., Kakyo M., Tokui T., Nakagomi R., Nishio T., Nakai D., Nomura H., Unno M., Suzuki M., Naitoh T., Matsuno S., Yawo H. J. Biol. Chem. 274:17159-17163(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, VARIANTS LYS-152 AND ASN-241. Tissue: Liver.
[2]	"A novel human hepatic organic anion transporting polypeptide (OATP2). Identification of a liver-specific human organic anion transporting polypeptide and identification of rat and human hydroxymethylglutaryl-CoA reductase inhibitor transporters." Hsiang B.H., Zhu Y., Wang Z., Wu Y., Sasseville V., Yang W.-P., Kirchgessner T.G. J. Biol. Chem. 274:37161-37168(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, VARIANT ASP-130. Tissue: Liver.
[3]	"A novel human organic anion transporting polypeptide localized to the basolateral hepatocyte membrane." Koenig J., Cui Y., Nies A.T., Keppler D. Am. J. Physiol. 278:G156-G164(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[4]	Nezu J. Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[5]	"Localization and genomic organization of a new hepatocellular organic anion transporting polypeptide." Koenig J., Cui Y., Nies A.T., Keppler D. J. Biol. Chem. 275:23161-23168(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[6]	SeattleSNPs variation discovery resource Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT ASP-130.
[7]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Mammary gland.
[8]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[9]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANTS ASP-130 AND THR-155.
[10]	"Complete OATP1B1 and OATP1B3 deficiency causes human Rotor syndrome by interrupting conjugated bilirubin reuptake into the liver." van de Steeg E., Stranecky V., Hartmannova H., Noskova L., Hrebicek M., Wagenaar E., van Esch A., de Waart D.R., Oude Elferink R.P., Kenworthy K.E., Sticova E., al-Edreesi M., Knisely A.S., Kmoch S., Jirsa M., Schinkel A.H. J. Clin. Invest. 122:519-528(2012) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, INVOLVEMENT IN HBLRR.
[11]	"Polymorphisms in OATP-C: identification of multiple allelic variants associated with altered transport activity among European- and African-Americans." Tirona R.G., Leake B.F., Merino G., Kim R.B. J. Biol. Chem. 276:35669-35675(2001) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANTS LEU-73; ALA-82; ASP-130; LYS-152; THR-155; GLY-156; ALA-174; ASN-241; THR-353; ASP-432; GLY-462; ALA-488; GLY-655 AND GLY-667, CHARACTERIZATION.
[12]	"A naturally occurring mutation in the SLC21A6 gene causing impaired membrane localization of the hepatocyte uptake transporter." Michalski C., Cui Y., Nies A.T., Nuessler A.K., Neuhaus P., Zanger U.M., Klein K., Eichelbaum M., Keppler D., Koenig J. J. Biol. Chem. 277:43058-43063(2002) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION OF VARIANTS ASP-130; THR-155 AND ARG-193, FUNCTION, SUBCELLULAR LOCATION.
[13]	"Genetic polymorphisms of human organic anion transporters OATP-C (SLC21A6) and OATP-B (SLC21A9): allele frequencies in the Japanese population and functional analysis." Nozawa T., Nakajima M., Tamai I., Noda K., Nezu J., Sai Y., Tsuji A., Yokoi T. J. Pharmacol. Exp. Ther. 302:804-813(2002) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANTS ASP-130; LYS-152; ALA-174 AND ASN-241.
+	Additional computationally mapped references.

Web resources

SeattleSNPs

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF060500 mRNA. Translation: AAD38323.1. AF205071 mRNA. Translation: AAF20212.1. AJ132573 mRNA. Translation: CAB62577.1. AB026257 mRNA. Translation: BAA78639.1. AJ400749 , AJ400750, AJ400751, AJ400752, AJ400753, AJ400754, AJ400755, AJ400756, AJ400757, AJ400758, AJ400759, AJ400760, AJ400761, AJ400762 Genomic DNA. Translation: CAB97007.1. AY945934 Genomic DNA. Translation: AAX20114.1. AK312970 mRNA. Translation: BAG35809.1. CH471094 Genomic DNA. Translation: EAW96420.1. BC114376 mRNA. Translation: AAI14377.1.
IPI	IPI00295376.
RefSeq	NP_006437.3. NM_006446.4.
UniGene	Hs.449738.
3D structure databases
ProteinModelPortal	Q9Y6L6.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000256958.
Protein family/group databases
TCDB	2.A.60.1.5. organo anion transporter (OAT) family.
PTM databases
PhosphoSite	Q9Y6L6.
Polymorphism databases
DMDM	12643959.
Proteomic databases
PaxDb	Q9Y6L6.
PRIDE	Q9Y6L6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000256958; ENSP00000256958; ENSG00000134538.
GeneID	10599.
KEGG	hsa:10599.
UCSC	uc001req.4. human.
Organism-specific databases
CTD	10599.
GeneCards	GC12P021284.
HGNC	HGNC:10959. SLCO1B1.
HPA	HPA050892.
MIM	237450. phenotype. 604843. gene.
neXtProt	NX_Q9Y6L6.
Orphanet	240851. Atorvastatin toxicity. 240903. Pravastatin toxicity. 240909. Rosuvastatin toxicity. 3111. Rotor syndrome. 240913. Simvastatin toxicity. 240961. Susceptibility to adverse reaction due to atorvastatin treatment. 240981. Susceptibility to adverse reaction due to pravastatin treatment. 240983. Susceptibility to adverse reaction due to rosuvastatin treatment. 240985. Susceptibility to adverse reaction due to simvastatin treatment.
PharmGKB	PA134865839.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG322696.
HOGENOM	HOG000231269.
HOVERGEN	HBG063896.
InParanoid	Q9Y6L6.
KO	K05043.
OMA	SIIHIER.
OrthoDB	EOG4V6ZGC.
PhylomeDB	Q9Y6L6.
Enzyme and pathway databases
Reactome	REACT_111217. Metabolism. REACT_15518. Transmembrane transport of small molecules.
Gene expression databases
Bgee	Q9Y6L6.
CleanEx	HS_LST1. HS_SLCO1B1.
Genevestigator	Q9Y6L6.
Family and domain databases
InterPro	IPR002350. Kazal_dom. IPR020846. MFS_dom. IPR016196. MFS_dom_general_subst_transpt. IPR004156. OA_transporter. [Graphical view]
PANTHER	PTHR11388. PTHR11388. 1 hit.
Pfam	PF07648. Kazal_2. 1 hit. PF03137. OATP. 1 hit. [Graphical view]
SMART	SM00280. KAZAL. 1 hit. [Graphical view]
SUPFAM	SSF103473. MFS_gen_substrate_transporter. 1 hit.
TIGRFAMs	TIGR00805. oat. 1 hit.
PROSITE	PS51465. KAZAL_2. 1 hit. PS50850. MFS. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	Q9Y6L6.
ChEMBL	CHEMBL1697668.
ChiTaRS	SLCO1B1. human.
DrugBank	DB00390. Digoxin. DB01241. Gemfibrozil. DB00175. Pravastatin.
GenomeRNAi	10599.
NextBio	40250.
SOURCE	Search...

Entry information

Entry name

SO1B1_HUMAN

Accession

Primary (citable) accession number: Q9Y6L6
Secondary accession number(s): B2R7G2 , Q29R64, Q9NQ37, Q9UBF3, Q9UH89

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	May 1, 2013
This is version 114 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.